photosystem ii protein complex

Summary

Summary: A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to catalyze the splitting of WATER into DIOXYGEN and of reducing equivalents of HYDROGEN.

Top Publications

  1. ncbi Dynamics of photosystem II: a proteomic approach to thylakoid protein complexes
    E M Aro
    Department of Biology, University of Turku, FIN 20014 Turku, Finland
    J Exp Bot 56:347-56. 2005
  2. ncbi Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å
    Yasufumi Umena
    Department of Chemistry, Graduate School of Science, Osaka City University, 3 3 138 Sugimoto, Sumiyoshi, Osaka 558 8585, Japan
    Nature 473:55-60. 2011
  3. pmc Production and scavenging of reactive oxygen species in chloroplasts and their functions
    Kozi Asada
    Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama 729 0292, Japan
    Plant Physiol 141:391-6. 2006
  4. pmc Photoprotective energy dissipation involves the reorganization of photosystem II light-harvesting complexes in the grana membranes of spinach chloroplasts
    Matthew P Johnson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E14NS, United Kingdom
    Plant Cell 23:1468-79. 2011
  5. ncbi A pigment-binding protein essential for regulation of photosynthetic light harvesting
    X P Li
    Department of Plant and Microbial Biology, University of California, Berkeley 94720 3102, USA
    Nature 403:391-5. 2000
  6. ncbi Architecture of the photosynthetic oxygen-evolving center
    Kristina N Ferreira
    Department of Biological Sciences, Imperial College London, London, SW7 2AZ, UK
    Science 303:1831-8. 2004
  7. ncbi The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II
    Peter Jahns
    Plant Biochemistry, Heinrich Heine University Dusseldorf, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Biochim Biophys Acta 1817:182-93. 2012
  8. pmc Recent advances in understanding the assembly and repair of photosystem II
    Peter J Nixon
    Department of Life Sciences, Imperial College London, London SW7 2AZ, UK
    Ann Bot 106:1-16. 2010
  9. ncbi Marine ecosystems: bacterial photosynthesis genes in a virus
    Nicholas H Mann
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Nature 424:741. 2003
  10. ncbi Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride
    Albert Guskov
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Nat Struct Mol Biol 16:334-42. 2009

Detail Information

Publications396 found, 100 shown here

  1. ncbi Dynamics of photosystem II: a proteomic approach to thylakoid protein complexes
    E M Aro
    Department of Biology, University of Turku, FIN 20014 Turku, Finland
    J Exp Bot 56:347-56. 2005
    ..Moreover, a two-dimensional Blue-native gel map of thylakoid membrane protein complexes, and their modification in the grana and stroma lamellae during a high-light treatment, is presented...
  2. ncbi Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å
    Yasufumi Umena
    Department of Chemistry, Graduate School of Science, Osaka City University, 3 3 138 Sugimoto, Sumiyoshi, Osaka 558 8585, Japan
    Nature 473:55-60. 2011
    ..The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail...
  3. pmc Production and scavenging of reactive oxygen species in chloroplasts and their functions
    Kozi Asada
    Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama 729 0292, Japan
    Plant Physiol 141:391-6. 2006
  4. pmc Photoprotective energy dissipation involves the reorganization of photosystem II light-harvesting complexes in the grana membranes of spinach chloroplasts
    Matthew P Johnson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E14NS, United Kingdom
    Plant Cell 23:1468-79. 2011
    ..It is demonstrated that these changes occur rapidly and reversibly within 5 min of illumination and dark relaxation, are dependent on ΔpH, and are enhanced by the deepoxidation of violaxanthin to zeaxanthin...
  5. ncbi A pigment-binding protein essential for regulation of photosynthetic light harvesting
    X P Li
    Department of Plant and Microbial Biology, University of California, Berkeley 94720 3102, USA
    Nature 403:391-5. 2000
    ..These results indicate that PsbS may be the site for nonphotochemical quenching, a finding that has implications for the functional evolution of pigment-binding proteins...
  6. ncbi Architecture of the photosynthetic oxygen-evolving center
    Kristina N Ferreira
    Department of Biological Sciences, Imperial College London, London, SW7 2AZ, UK
    Science 303:1831-8. 2004
    ..The details of the surrounding coordination sphere of the metal cluster and the implications for a possible oxygen-evolving mechanism are discussed...
  7. ncbi The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II
    Peter Jahns
    Plant Biochemistry, Heinrich Heine University Dusseldorf, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Biochim Biophys Acta 1817:182-93. 2012
    ..This article is part of a Special Issue entitled: Photosystem II...
  8. pmc Recent advances in understanding the assembly and repair of photosystem II
    Peter J Nixon
    Department of Life Sciences, Imperial College London, London SW7 2AZ, UK
    Ann Bot 106:1-16. 2010
    ..Understanding these processes might lead to the development of plants with improved growth characteristics especially under conditions of abiotic stress...
  9. ncbi Marine ecosystems: bacterial photosynthesis genes in a virus
    Nicholas H Mann
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Nature 424:741. 2003
  10. ncbi Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride
    Albert Guskov
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195 Berlin, Germany
    Nat Struct Mol Biol 16:334-42. 2009
    ..The chloride position suggests a role in proton-transfer reactions because it is bound through a putative water molecule to the Mn(4)Ca cluster at a distance of 6.5 A and is close to two possible proton channels...
  11. ncbi Singlet oxygen in plants: production, detoxification and signaling
    Christian Triantaphylidès
    CEA, IBEB, SBVME, Laboratoire d Ecophysiologie Moléculaire des Plantes, 13108 Saint Paul Lez Durance, France
    Trends Plant Sci 14:219-28. 2009
    ..Compared with other ROS, (1)O(2) has received relatively little attention, but recent developments indicate that it has a crucial role in the responses of plants to light...
  12. pmc Prevalence and evolution of core photosystem II genes in marine cyanobacterial viruses and their hosts
    Matthew B Sullivan
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 4:e234. 2006
    ..This generates genetic diversity among the phage, which serves as a reservoir for their hosts, and in turn influences photosystem evolution...
  13. ncbi Chlorophyll fluorescence: a probe of photosynthesis in vivo
    Neil R Baker
    Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, United Kingdom
    Annu Rev Plant Biol 59:89-113. 2008
    ..Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered...
  14. ncbi How do environmental stresses accelerate photoinhibition?
    Shunichi Takahashi
    Molecular Plant Physiology Group and Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biological Sciences, Australian National University, PO Box 475, Canberra, ACT 0200, Australia
    Trends Plant Sci 13:178-82. 2008
    ..The latter results in the creation of reactive oxygen species (ROS), which in turn block the synthesis of PSII proteins in chloroplasts...
  15. ncbi Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium
    I Berman-Frank
    Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ 08901, USA
    Science 294:1534-7. 2001
    ....
  16. ncbi Water-splitting chemistry of photosystem II
    James P McEvoy
    Department of Chemistry, Yale University, P O Box 208107, New Haven, Connecticut 06520 8107, USA
    Chem Rev 106:4455-83. 2006
  17. pmc Zeaxanthin has enhanced antioxidant capacity with respect to all other xanthophylls in Arabidopsis leaves and functions independent of binding to PSII antennae
    Michel Havaux
    CEA Cadarache, Institut de Biologie Environnementale et Biotechnologie, Laboratoire d Ecophysiologie Moléculaire des Plantes, F 13108 Saint Paul lez Durance, France
    Plant Physiol 145:1506-20. 2007
    ..The capacity of zeaxanthin to protect thylakoid membrane lipids is comparable to that of vitamin E but noticeably higher than that of all other xanthophylls of Arabidopsis leaves...
  18. ncbi State transitions and light adaptation require chloroplast thylakoid protein kinase STN7
    Stephane Bellafiore
    Department of Molecular Biology, University of Geneva, 30, Quai Ernest Ansermet, 1211 Geneva, Switzerland
    Nature 433:892-5. 2005
    ..In stn7 mutant plants the plastoquinone pool is more reduced and growth is impaired under changing light conditions, indicating that STN7, and probably state transitions, have an important role in response to environmental changes...
  19. ncbi Photoprotection in plants: a new light on photosystem II damage
    Shunichi Takahashi
    Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Canberra, ACT 2601, Australia
    Trends Plant Sci 16:53-60. 2011
    ....
  20. ncbi Photoinhibition of photosystem II under environmental stress
    Norio Murata
    National Institute for Basic Biology, Okazaki 444 8585, Japan
    Biochim Biophys Acta 1767:414-21. 2007
    ..We postulate that most types of environmental stress inhibit the fixation of CO(2) with the resultant generation of ROS, which, in turn, inhibit protein synthesis...
  21. ncbi Heat stress: an overview of molecular responses in photosynthesis
    Suleyman I Allakhverdiev
    Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    Photosynth Res 98:541-50. 2008
    ..In this review we summarize the recent progress in the studies of molecular mechanisms involved during moderate heat stress on the photosynthetic machinery, especially in PSII...
  22. ncbi Architecture of a charge-transfer state regulating light harvesting in a plant antenna protein
    Tae Kyu Ahn
    Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Science 320:794-7. 2008
    ....
  23. ncbi Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis
    Reto J Strasser
    Weed Research Laboratory, Nanjing Agricultural University, Nanjing 210095, China
    Biochim Biophys Acta 1797:1313-26. 2010
    ..rhodopensis is mainly based on mechanism(s) that lead to inactivation of photosystem II reaction centres (transformation to heat sinks), triggered already by a small RWC decrease...
  24. ncbi The cost of photoinhibition
    John A Raven
    Division of Plant Sciences, University of Dundee at SCRI, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK
    Physiol Plant 142:87-104. 2011
    ..e. the competitive inhibition of the carboxylase activity of ribulose bisphosphate carboxylase-oxygenase by oxygen via the oxygenase activity, and oxygen damage to nitrogenase in diazotrophic organisms...
  25. ncbi The PsbS protein controls the macro-organisation of photosystem II complexes in the grana membranes of higher plant chloroplasts
    Sami Kereïche
    Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
    FEBS Lett 584:759-64. 2010
    ..It is proposed that PsbS controls the macro-organisation of the grana membrane, providing an explanation of its role in NPQ...
  26. ncbi Heterogeneous behavior of PSII in soybean (Glycine max) leaves with identical PSII photochemistry efficiency under different high temperature treatments
    Pengmin Li
    State Key Lab of Crop Biology College of Life Sciences, Shandong Agricultural University, Tai an, Shandong 271018, China
    J Plant Physiol 166:1607-15. 2009
    ....
  27. pmc Investigating the early stages of photosystem II assembly in Synechocystis sp. PCC 6803: isolation of CP47 and CP43 complexes
    Marko Boehm
    Department of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Biol Chem 286:14812-9. 2011
    ..Overall, our data support the view that CP47 and CP43 form preassembled pigment-protein complexes in vivo before their incorporation into the PSII complex...
  28. ncbi Light-harvesting antenna composition controls the macrostructure and dynamics of thylakoid membranes in Arabidopsis
    Tomasz K Goral
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E14NS, UK
    Plant J 69:289-301. 2012
    ..Thus the presence of PsbS leads to an increase in the fluidity of the membrane, accelerating the re-organization of the photosystem II macrostructure that is necessary for induction of non-photochemical quenching...
  29. pmc Cooperative D1 degradation in the photosystem II repair mediated by chloroplastic proteases in Arabidopsis
    Yusuke Kato
    Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710 0046, Japan
    Plant Physiol 159:1428-39. 2012
    ..Together, our data demonstrate in vivo cooperative degradation of D1, in which Deg cleavage assists FtsH processive degradation under photoinhibitory conditions...
  30. ncbi Assembling and maintaining the Photosystem II complex in chloroplasts and cyanobacteria
    Josef Komenda
    Institute of Microbiology, Laboratory of Photosynthesis, Opatovicky mlyn, Trebon, Czech Republic
    Curr Opin Plant Biol 15:245-51. 2012
    ..The chloroplastic Deg proteases play a supplementary role in PSII repair...
  31. pmc Structural basis of cyanobacterial photosystem II Inhibition by the herbicide terbutryn
    Matthias Broser
    Institut für Chemie Max Volmer Laboratorium für Biophysikalische Chemie, Technische Universitat Berlin, Berlin, Germany
    J Biol Chem 286:15964-72. 2011
    ..Kern, J., Gabdulkhakov, A., Broser, M., Zouni, A., and Saenger, W. (2009) Nat. Struct. Mol. Biol. 16, 334-342). This discovery is discussed in the context of proton transfer to the lumen...
  32. pmc A nuclear-encoded protein of prokaryotic origin is essential for the stability of photosystem II in Arabidopsis thaliana
    J Meurer
    Heinrich Heine Universitat, Universitatsstrasse 1, 40225 Dusseldorf, Germany
    EMBO J 17:5286-97. 1998
    ..We conclude that HCF136 encodes a stability and/or assembly factor of PSII which dates back to the cyanobacterial-like endosymbiont that led to the plastids of the present photosynthetic eukaryotes...
  33. pmc Antisense inhibition of the photosynthetic antenna proteins CP29 and CP26: implications for the mechanism of protective energy dissipation
    J Andersson
    Umea Plant Science Centre, Department of Plant Physiology, Umea University, S 901 87 Umea, Sweden
    Plant Cell 13:1193-204. 2001
    ..High-light-induced violaxanthin deepoxidation to zeaxanthin was not affected, although the pool size of these pigments was decreased slightly. We conclude that CP29 and CP26 are unlikely to be sites for nonphotochemical quenching...
  34. pmc The solar action spectrum of photosystem II damage
    Shunichi Takahashi
    Australian Research Council Centre of Excellence in Plant Energy Biology, Australian National University, Canberra, Australian Capital Territory 2601, Australia
    Plant Physiol 153:988-93. 2010
    ..Our results suggest that photodamage to PSII under sunlight is primarily associated with UV rather than photosynthetically active light wavelengths...
  35. ncbi Singlet oxygen production in photosystem II and related protection mechanism
    Anja Krieger-Liszkay
    CEA, Institut de Biologie et Technologies de Saclay, CNRS URA 2096, Service de Bioénergétique Biologie Structurale et Mécanisme, 91191 Gif sur Yvette Cedex, France
    Photosynth Res 98:551-64. 2008
    ..If not quenched, it can trigger the up-regulation of genes, which are involved in the molecular defence response of photosynthetic organisms against photo-oxidative stress...
  36. ncbi Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration
    L Cournac
    Commissariat à l Energie Atomique CEA Cadarache, Départment d Ecophysiologie Végétale et de Microbiologie DEVM, laboratoire d écophysiologie de la photosynthèse, 13108 Saint Paul Lez Durance, France
    J Biol Chem 275:17256-62. 2000
    ..Clues to the identity of the protein implied in this process are given by pharmacological and immunological similarities with a protein (IMMUTANS) identified in Arabidopsis chloroplasts...
  37. ncbi The extrinsic proteins of Photosystem II
    Johnna L Roose
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Photosynth Res 92:369-87. 2007
    ..Additionally, the role that these proteins play in the dynamic life cycle of PSII will be discussed...
  38. ncbi D1-protein dynamics in photosystem II: the lingering enigma
    Marvin Edelman
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel
    Photosynth Res 98:609-20. 2008
    ..This review will touch on some treaded areas of D1 research that have, so far, defied clear resolution, as well as cutting edge research on mechanisms and consequences of D1 protein degradation...
  39. pmc Light-induced dissociation of an antenna hetero-oligomer is needed for non-photochemical quenching induction
    Nico Betterle
    Dipartimento Scientifico e Tecnologico, Universita di Verona, Strada Le Grazie 15, I 37134 Verona, Italy
    J Biol Chem 284:15255-66. 2009
    ..These changes are reversible and do not require protein synthesis/degradation, thus allowing for changes in PSII antenna size and adaptation to rapidly changing environmental conditions...
  40. pmc The nucleus-encoded HCF107 gene of Arabidopsis provides a link between intercistronic RNA processing and the accumulation of translation-competent psbH transcripts in chloroplasts
    S Felder
    Institut für Entwicklungs und Molekularbiologie der Pflanzen, Heinrich Heine Universitat, Universitatstrasse 1, 40225 Dusseldorf, Germany
    Plant Cell 13:2127-41. 2001
    ....
  41. ncbi Crystal structure of photosystem II from Synechococcus elongatus at 3.8 A resolution
    A Zouni
    Max Volmer Institut für Biophysikalische Chemie und Biochemie, Technische Universitat Berlin, Germany
    Nature 409:739-43. 2001
    ..The larger subunits are assigned and the locations and orientations of the cofactors are defined. We also provide new information on the position, size and shape of the manganese cluster, which catalyzes water oxidation...
  42. ncbi The nuclear gene HCF107 encodes a membrane-associated R-TPR (RNA tetratricopeptide repeat)-containing protein involved in expression of the plastidial psbH gene in Arabidopsis
    Aniruddha P Sane
    Institut fuer Entwicklungs und Molekular Biologie der Pflanzen, Heinrich Heine Universitaet, D 40225 Duesseldorf, Germany
    Plant J 42:720-30. 2005
    ..HCF107 thus represents a new member of the growing helical repeat family of proteins that seem to play a gene-specific role in regulating plastidial gene expression and biogenesis...
  43. pmc The violaxanthin cycle protects plants from photooxidative damage by more than one mechanism
    M Havaux
    Commissariat à l Energie Atomique Cadarache, Département d Ecophysiologie Végétale et de Microbiologie, F 13108 Saint Paul lez Durance, France
    Proc Natl Acad Sci U S A 96:8762-7. 1999
    ..Our results demonstrate that the violaxanthin cycle specifically protects thylakoid membrane lipids against photooxidation. Part of this protection involves a mechanism other than quenching of singlet excited chlorophylls...
  44. pmc Functional architecture of higher plant photosystem II supercomplexes
    Stefano Caffarri
    Faculte des Sciences Luminy, Laboratoire de Genetique et Biophysique des Plantes, Universite Aix Marseille, Marseille, France
    EMBO J 28:3052-63. 2009
    ..The functional implications of these findings are discussed and allow redefining previous suggestions on PSII energy transfer, assembly, photoinhibition, state transition and non-photochemical quenching...
  45. ncbi Regulation of photosynthetic light harvesting involves intrathylakoid lumen pH sensing by the PsbS protein
    Xiao Ping Li
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
    J Biol Chem 279:22866-74. 2004
    ..Furthermore, based on the extent of qE inhibition in the mutants, photochemical and nonphotochemical quenching processes of photosystem II were associated with distinct chlorophyll fluorescence life-time distribution components...
  46. ncbi Identification of a mechanism of photoprotective energy dissipation in higher plants
    Alexander V Ruban
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK
    Nature 450:575-8. 2007
    ..We suggest that this is the principal mechanism of photoprotection...
  47. pmc Identification of cis-acting RNA leader elements required for chloroplast psbD gene expression in Chlamydomonas
    J Nickelsen
    Lehrstuhl für Allgemeine Botanik, Ruhr Universitat Bochum, 44780 Bochum, Germany
    Plant Cell 11:957-70. 1999
    ..The singular organization of the psbD 5' untranslated region could play an important role in the control of initiation of psbD mRNA translation...
  48. ncbi A new paradigm for the action of reactive oxygen species in the photoinhibition of photosystem II
    Yoshitaka Nishiyama
    Cell Free Science and Technology Research Center and Satellite Venture Business Laboratory, Ehime University, Bunkyo cho, Matsuyama, Japan
    Biochim Biophys Acta 1757:742-9. 2006
    ..Inhibition of the repair of PSII by ROS is accelerated by the deceleration of the Calvin cycle that occurs when the availability of CO(2) is limited. In this review, we present a new paradigm for the action of ROS in photoinhibition...
  49. pmc Arabidopsis mutants deleted in the light-harvesting protein Lhcb4 have a disrupted photosystem II macrostructure and are defective in photoprotection
    Silvia de Bianchi
    Dipartimento di Biotecnologie, Universita di Verona, 37134 Verona, Italy
    Plant Cell 23:2659-79. 2011
    ..This suggests that Lhcb4 is unique among photosystem II antenna proteins and determinant for photosystem II macro-organization and photoprotection...
  50. ncbi Mitochondrial alternative oxidase pathway protects plants against photoinhibition by alleviating inhibition of the repair of photodamaged PSII through preventing formation of reactive oxygen species in Rumex K-1 leaves
    Li Tao Zhang
    State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai an 271018, Shandong, China
    Physiol Plant 143:396-407. 2011
    ..All these results suggest that the AOX pathway plays an important role in the protection of plants against photoinhibition by minimizing the inhibition of the repair of the photodamaged PSII through preventing the over-production of ROS...
  51. ncbi Genetic engineering of the biosynthesis of glycinebetaine enhances thermotolerance of photosystem II in tobacco plants
    Xinghong Yang
    Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People s Republic of China
    Planta 225:719-33. 2007
    ....
  52. ncbi Rapid regulation of light harvesting and plant fitness in the field
    Carsten Külheim
    Umea Plant Science Center, Department of Plant Physiology, Umea University, SE 901 87 Umea, Sweden
    Science 297:91-3. 2002
    ....
  53. pmc HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs
    Karin Meierhoff
    Heinrich Heine Universitat, Institut für Entwicklungs und Molekularbiologie der Pflanzen, 40225 Dusseldorf, Germany
    Plant Cell 15:1480-95. 2003
    ..The protein possibly exists in the chloroplast as a homodimer and is not associated with other proteins to form a high molecular mass complex...
  54. ncbi Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance
    Abdallah Oukarroum
    Bioenergetics and Microbiology laboratory, University of Geneva, Jussy Geneva, Switzerland
    Physiol Plant 137:188-99. 2009
    ..Lannaceur, under well-watered conditions, was more heat tolerant than Aït Baha. However, this difference was lost following drought stress. Drought stress considerably increased the thermostability of PS II of both varieties...
  55. ncbi Photosystem II core phosphorylation and photosynthetic acclimation require two different protein kinases
    Vera Bonardi
    Botanisches Institut, Department Biologie I, Ludwig Maximilians Universitat, Menzinger Strasse 67, 80638 Munchen, Germany
    Nature 437:1179-82. 2005
    ..Therefore the phosphorylation of LHCII, or of an unknown substrate of STN7, is also crucial for the control of photosynthetic gene expression...
  56. ncbi State transitions--the molecular remodeling of photosynthetic supercomplexes that controls energy flow in the chloroplast
    Jun Minagawa
    Nattional Institute for Basic Biology, Okazaki, Japan
    Biochim Biophys Acta 1807:897-905. 2011
    ..This article is part of a Special Issue entitled: Regulation of Electron Transport in Chloroplasts...
  57. pmc Kinetics and mechanism of electron transfer in intact photosystem II and in the isolated reaction center: pheophytin is the primary electron acceptor
    A R Holzwarth
    Max Planck Institut fur Bioanorganische Chemie, Stiftstrasse 34 36, D 45470 Mulheim a d Ruhr, Germany
    Proc Natl Acad Sci U S A 103:6895-900. 2006
    ..The effective rate constant of primary electron transfer from the equilibrated RC* excited state is 170-180 ns(-1), and the rate constant of secondary electron transfer is 120-130 ns(-1)...
  58. pmc Excitation energy transfer and trapping in higher plant Photosystem II complexes with different antenna sizes
    Stefano Caffarri
    Aix Marseille Universite, Laboratoire de Genetique et Biophysique des Plantes, Marseille, France
    Biophys J 100:2094-103. 2011
    ..We conclude that the efficiency of the nonphotochemical quenching process is hardly dependent on the exact location of a quencher within the supercomplexes...
  59. ncbi Experimental in vivo measurements of light emission in plants: a perspective dedicated to David Walker
    Hazem M Kalaji
    Department of Plant Physiology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences SGGW, Nowoursynowska 159, 02 776 Warsaw, Poland
    Photosynth Res 114:69-96. 2012
    ..A review concerning the history of fluorometers is also presented...
  60. pmc Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii
    Hiroko Takahashi
    Department of Biology, Faculty of Science, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Proc Natl Acad Sci U S A 103:477-82. 2006
    ....
  61. ncbi Primary photophysical processes in photosystem II: bridging the gap between crystal structure and optical spectra
    Thomas Renger
    Institut fur Theoretische Physik, Johannes Kepler Universitat, Altenberger Strasse 69, 4040 Linz, Austria
    Chemphyschem 11:1141-53. 2010
    ..This system does not suffer from the spectral congestion problem and, therefore, represents an interesting model system. The final part of this Minireview provides a discussion of challenging problems to be solved in the future...
  62. ncbi Charge separation is virtually irreversible in photosystem II core complexes with oxidized primary quinone acceptor
    C D van der Weij-de Wit
    Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    J Phys Chem A 115:3947-56. 2011
    ..We conclude that the energy transfer from the CP43/CP47 antenna to the RC is the dominant factor in the total charge separation kinetics in intact PSII cores...
  63. ncbi Revealing the structure of the photosystem II chlorophyll binding proteins, CP43 and CP47
    J Barber
    Biochemistry Department, Wolfson Laboratories, Imperial College of Science, Technology and Medicine, SW7 2AY, London, UK
    Biochim Biophys Acta 1459:239-47. 2000
    ..Improved structural resolution and reconsiderations of the orientation of the porphyrin rings will allow further refinement of the model...
  64. ncbi Photosystem II photochemistry, photoinhibition, and the xanthophyll cycle in heat-stressed rice leaves
    Yan Yin
    Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, PR China
    J Plant Physiol 167:959-66. 2010
    ..Our results suggest that the xanthophyll cycle plays an important role in protecting PSII against heat-induced photoinhibition by an increase in the ascorbate pool in the chloroplast...
  65. pmc Live-cell imaging of photosystem II antenna dissociation during state transitions
    Masakazu Iwai
    Institute of Low Temperature Science, Hokkaido University, N19 W8, Sapporo, Hokkaido 060 0819, Japan
    Proc Natl Acad Sci U S A 107:2337-42. 2010
    ..Possible implications of the unbound phospho-LHCII on energy dissipation are discussed...
  66. ncbi Optical biosensors for environmental monitoring based on computational and biotechnological tools for engineering the photosynthetic D1 protein of Chlamydomonas reinhardtii
    Maria Teresa Giardi
    Institute of Crystallography, Area of Research of Rome, Department of Agrofood, CNR, Via Salaria km 29 300, 00015, Monterotondo Scalo, Rome, Italy
    Biosens Bioelectron 25:294-300. 2009
    ..8 x 10(-11) and 3.0 x 10(-9), depending on the target analyte. In addition, biomediator regeneration without obvious deterioration in performance was demonstrated...
  67. pmc Chemical quenching of singlet oxygen by carotenoids in plants
    Fanny Ramel
    Commissariat à l Énergie Atomique et aux Énergies Alternatives, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, F 13108 Saint Paul lez Durance, France
    Plant Physiol 158:1267-78. 2012
    ..This carotenoid metabolite provides an early index of (1)O(2) production in leaves, the occurrence of which precedes the accumulation of fatty acid oxidation products...
  68. ncbi Principles, efficiency, and blueprint character of solar-energy conversion in photosynthetic water oxidation
    Holger Dau
    Freie Universitat Berlin, FB Physik, Arnimallee 14, D 14195 Berlin, Germany
    Acc Chem Res 42:1861-70. 2009
    ..In conclusion, photosynthetic water oxidation works at high efficiency and thus can serve as both an inspiring model and a benchmark in the development of future technologies for production of solar fuels...
  69. pmc Spectroscopic properties of reaction center pigments in photosystem II core complexes: revision of the multimer model
    Grzegorz Raszewski
    Institut fur Chemie und Biochemie Kristallographie, Freie Universitat Berlin, Berlin, Germany
    Biophys J 95:105-19. 2008
    ....
  70. ncbi The PsbS protein controls the organization of the photosystem II antenna in higher plant thylakoid membranes
    Anett Z Kiss
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 283:3972-8. 2008
    ..It is proposed that PsbS regulates the interaction between LHCII and PSII in the grana membranes, explaining how it acts as a pH-dependent trigger of the conformational changes within the PSII light harvesting system that result in NPQ...
  71. ncbi Arabidopsis plants lacking PsbS protein possess photoprotective energy dissipation
    Matthew P Johnson
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End, Bancroft Road, London E1 4NS, UK
    Plant J 61:283-9. 2010
    ....
  72. pmc On the regulation of photosynthesis by excitonic interactions between carotenoids and chlorophylls
    Stefan Bode
    Technische Universitat Braunschweig, Institute for Physical and Theoretical Chemistry, Department for Biophysical Chemistry, Hans Sommer Strasse 10, 38106 Braunschweig, Germany
    Proc Natl Acad Sci U S A 106:12311-6. 2009
    ..These findings support a photophysical mechanism in which short-living, low excitonic carotenoid-chlorophyll states serve as traps and dissipation valves for excess excitation energy...
  73. ncbi LHCII is an antenna of both photosystems after long-term acclimation
    Emilie Wientjes
    Department of Physics and Astronomy, VU University Amsterdam, 1081 HV Amsterdam, The Netherlands
    Biochim Biophys Acta 1827:420-6. 2013
    ..Finally, time-resolved fluorescence measurements on the photosynthetic thylakoid membranes show that LHCII is even a more efficient light harvester when associated with Photosystem I than with Photosystem II...
  74. pmc Operon flv4-flv2 provides cyanobacterial photosystem II with flexibility of electron transfer
    Pengpeng Zhang
    Department of Biochemistry and Food Chemistry, Molecular Plant Biology, University of Turku, 20520 Turku, Finland
    Plant Cell 24:1952-71. 2012
    ..The function of the flv4-flv2 operon provides many β-cyanobacteria with a so far unknown photoprotection mechanism that evolved in parallel with oxygen-evolving PSII...
  75. ncbi Biochemistry. Water photolysis in biology
    A W Rutherford
    Service of Bioenergetics, CNRS URA 2096, Departement de Biologie Joliot Curie, CEA Saclay, 91191 Gif sur Yvette, France
    Science 303:1782-4. 2004
  76. ncbi Thylakoid protein phosphorylation in dynamic regulation of photosystem II in higher plants
    Mikko Tikkanen
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    Biochim Biophys Acta 1817:232-8. 2012
    ..This article is part of a Special Issue entitled: Photosystem II...
  77. ncbi Molecular basis of photoprotection and control of photosynthetic light-harvesting
    Andrew A Pascal
    Service de Biophysique des Fonctions Membranaires, URA2096 CNRS and DBJC CEA, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Nature 436:134-7. 2005
    ..This provides a molecular basis for understanding the control of photosynthetic light-harvesting...
  78. ncbi Quality control of photosystem II: impact of light and heat stresses
    Yasusi Yamamoto
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Photosynth Res 98:589-608. 2008
    ..We suggest that phosphorylation and dephosphorylation of the D1 protein, as well as the binding of the extrinsic PsbO protein to Photosystem II, play regulatory roles in directing the damaged D1 protein to the two alternative pathways...
  79. pmc Photosynthetic responses to heat treatments at different temperatures and following recovery in grapevine (Vitis amurensis L.) leaves
    Hai Bo Luo
    Beijing Key Laboratory of Viticulture and Enology, Institute of Botany, Chinese Academy of Sciences, Beijing, People s Republic of China
    PLoS ONE 6:e23033. 2011
    ..Little is known about their combined responses to heat treatment at different temperatures and following recovery in grapevines (Vitis spp.) which are often grown in climates with high temperatures...
  80. pmc Dual role for tomato heat shock protein 21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation
    Inbal Neta-Sharir
    Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
    Plant Cell 17:1829-38. 2005
    ..This study demonstrates that a sHSP plays a role in plant development under normal growth conditions, in addition to its protective effect under stress conditions...
  81. pmc N-formylkynurenine as a marker of high light stress in photosynthesis
    Tina M Dreaden
    School of Chemistry and Biochemistry and Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    J Biol Chem 286:22632-41. 2011
    ..Reactive oxygen species reactions lead to oxidative damage of the reaction center, D1 protein turnover, and inhibition of electron transfer. Our results are consistent with a role for the CP43 NFK modification in photoinhibition...
  82. pmc LOW PSII ACCUMULATION1 is involved in efficient assembly of photosystem II in Arabidopsis thaliana
    Lianwei Peng
    Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    Plant Cell 18:955-69. 2006
    ..Thus, LPA1 appears to be an integral membrane chaperone that is required for efficient PSII assembly, probably through direct interaction with the PSII reaction center protein D1...
  83. ncbi Role of charge recombination processes in photodamage and photoprotection of the photosystem II complex
    Imre Vass
    Institute of Plant Biology, Biological Research Center, Szeged, Hungary
    Physiol Plant 142:6-16. 2011
    ..This mechanism is an important, but not exclusive, pathway of photodamage, and light-induced inactivation of the Mn cluster of water oxidation may occur in parallel with the singlet oxygen-dependent pathway...
  84. pmc Distinctive photosystem II photoinactivation and protein dynamics in marine diatoms
    Hongyan Wu
    Biology Department, Mount Allison University, Sackville, New Brunswick E4L1G7, Canada
    Plant Physiol 156:2184-95. 2011
    ....
  85. ncbi Carotenoid cation formation and the regulation of photosynthetic light harvesting
    Nancy E Holt
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Science 307:433-6. 2005
    ..These results indicate that energy transfer from chlorophyll molecules to a chlorophyllzeaxanthin heterodimer, which then undergoes charge separation, is the mechanism for excess energy dissipation during feedback deexcitation...
  86. ncbi The long-term response to fluctuating light quality is an important and distinct light acclimation mechanism that supports survival of Arabidopsis thaliana under low light conditions
    Raik Wagner
    Junior Research Group, Institute for General Botany and Plant Physiology, Friedrich Schiller University Jena, Dornburger Str 159, 07743 Jena, Germany
    Planta 228:573-87. 2008
    ..Thus, the LTR represents a distinct acclimation response in addition to other already known responses that clearly improves plant physiology under low light conditions resulting in a pronounced positive effect on plant fitness...
  87. pmc Different thermal sensitivity of the repair of photodamaged photosynthetic machinery in cultured Symbiodinium species
    Shunichi Takahashi
    Australian Research Council Center of Excellence in Plant Energy Biology, Research School of Biological Sciences, The Australian National University, Canberra, Australian Capital Territory, 2601 Australia
    Proc Natl Acad Sci U S A 106:3237-42. 2009
    ....
  88. ncbi In diatoms, the transthylakoid proton gradient regulates the photoprotective non-photochemical fluorescence quenching beyond its control on the xanthophyll cycle
    Johann Lavaud
    Group of Plant Ecophysiology, Biology Department, University of Konstanz, Germany
    Plant Cell Physiol 47:1010-6. 2006
    ..It confirms the NPQ model previously proposed for diatoms...
  89. pmc Hydrogen production in Chlamydomonas: photosystem II-dependent and -independent pathways differ in their requirement for starch metabolism
    Vincent Chochois
    Commissariat a l Energie Atomique, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, F 13108 Saint Paul lez Durance, France
    Plant Physiol 151:631-40. 2009
    ....
  90. pmc Control of protein life-span by N-terminal methionine excision
    Carmela Giglione
    Protein Maturation, Trafficking and Signaling, UPR2355, Centre National de la Recherche Scientifique, Institut des Sciences du Vegetal, Gif sur Yvette, France
    EMBO J 22:13-23. 2003
    ..Thus, plastid NME is a critical mechanism specifically influencing the life-span of photosystem II polypeptides. A general role of NME in modulating the half-life of key subsets of proteins is suggested...
  91. ncbi Coregulation of light-harvesting complex II phosphorylation and lhcb mRNA accumulation in winter rye
    S Pursiheimo
    Department of Biology, University of Turku, FIN-20014 Turku, Finland
    Plant J 26:317-27. 2001
    ..Instead (5) the redox status of the electron acceptors of photosystem I in the stromal compartment seems to be highly regulated and crucial for the regulation of lhcb gene expression in the nucleus...
  92. ncbi The importance of a highly active and DeltapH-regulated diatoxanthin epoxidase for the regulation of the PS II antenna function in diadinoxanthin cycle containing algae
    Reimund Goss
    Institut für Biologie I, Pflanzenphysiologie, Universitat Leipzig, Johannisallee 21 23, 04103 Leipzig, Germany
    J Plant Physiol 163:1008-21. 2006
    ..Due to the inability of Zx to quench fluorescence in the absence of the DeltapH a fast epoxidation of Zx to Vx in LL is not needed and is missing in Chlorella vulgaris...
  93. ncbi Protein synthesis is the primary target of reactive oxygen species in the photoinhibition of photosystem II
    Yoshitaka Nishiyama
    Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering and Institute for Environmental Science and Technology, Saitama University, Shimo okubo, Sakura ku, Saitama 338 8570, Japan
    Physiol Plant 142:35-46. 2011
    ..In this review, we present a newly established model of the mechanism and the physiological significance of repair in the regulation of the photoinhibition of PSII...
  94. ncbi Dynamics of reversible protein phosphorylation in thylakoids of flowering plants: the roles of STN7, STN8 and TAP38
    Paolo Pesaresi
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, I 20133 Milano, Italy
    Biochim Biophys Acta 1807:887-96. 2011
    ..This article is part of a Special Issue entitled: Regulation of Electron Transport in Chloroplasts...
  95. ncbi Localization of the small CAB-like proteins in photosystem II
    Danny Yao
    Department of Biochemistry, Umea University, Sweden
    J Biol Chem 282:267-76. 2007
    ..On the basis of these and other data presented, we suggest that members of the SCP family can associate with damaged PSII and can serve as a temporary pigment reservoir while PSII components are being replaced...
  96. ncbi Molecular recognition in thylakoid structure and function
    J F Allen
    Plant Biochemistry, Lund University, Box 117, SE 221 00, Lund, Sweden
    Trends Plant Sci 6:317-26. 2001
    ..High-resolution structural description of membrane protein-protein interactions is now required to understand thylakoid structure and regulation of photosynthesis...
  97. ncbi Xanthophyll biosynthetic mutants of Arabidopsis thaliana: altered nonphotochemical quenching of chlorophyll fluorescence is due to changes in Photosystem II antenna size and stability
    Heiko Lokstein
    Institut für Biologie Pflanzenphysiologie, Humboldt Universitat zu Berlin, Unter den Linden 6 Sitz Philippstr 13, D 10099, Berlin, Germany
    Biochim Biophys Acta 1553:309-19. 2002
    ....
  98. pmc The PPH1 phosphatase is specifically involved in LHCII dephosphorylation and state transitions in Arabidopsis
    Alexey Shapiguzov
    Department of Plant Biology, University of Geneva, 1211 Geneve 4, Switzerland
    Proc Natl Acad Sci U S A 107:4782-7. 2010
    ..These two proteins emerge as key players in the adaptation of the photosynthetic apparatus to changes in light quality and quantity...
  99. pmc Influence of the diadinoxanthin pool size on photoprotection in the marine planktonic diatom Phaeodactylum tricornutum
    Johann Lavaud
    Laboratoire Organismes Photosynthetiques et Environnement, Unité Mixte de Recherche Centre National de la Recherche Scientifique 8543, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Plant Physiol 129:1398-406. 2002
    ..The unusually rapid and effective photoprotection by the xanthophyll cycle in diatoms may help to explain their dominance in turbulent waters...
  100. ncbi Chloroplast translation regulation
    Julia Marin-Navarro
    Department of Cell Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Photosynth Res 94:359-74. 2007
    ..Finally, the localization of all these molecular events among the different chloroplast subcompartments appear to be a crucial component of the regulatory mechanisms of chloroplast gene expression...
  101. ncbi Photosynthetic acclimation: does the dynamic structure and macro-organisation of photosystem II in higher plant grana membranes regulate light harvesting states?
    Peter Horton
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, UK
    FEBS J 275:1069-79. 2008
    ....

Research Grants46

  1. Simultaneous Time-Resolved X-ray Spectroscopy and Crystallography: A Mechanistic
    Rosalie Tran; Fiscal Year: 2013
    ..abstract_text> ..
  2. Studies of redox-active sites in Photosystem II
    Victor S Batista; Fiscal Year: 2010
    ..abstract_text> ..
  3. Structure and Function of the Mn Oxygen-Evolving Complex
    Vittal Yachandra; Fiscal Year: 2013
    ..This proposal is directed towards the determination of the structure and mechanism of this critically important manganese enzyme. ..
  4. BIOLOGICAL METAL CLUSTERS--BIOPHYSICAL AND MODEL STUDIES
    Michael Maroney; Fiscal Year: 1999
    ..These goals will be met by using a combination of physical techniques applied to hydrogenases and to model systems that are designed to model specific aspects of the structure or function of the enzyme active site. ..
  5. Structure and Function of the Mn Oxygen-Evolving Complex
    Vittal Yachandra; Fiscal Year: 2009
    ..The oxidation states and electronic structure of the Mn complex are determined by Mn K- and L-edge, K[unreadable] emission, and X-ray resonant Raman spectroscopies. ..
  6. Synthetic Models of the Oxygen Evolving Complex of Photosystem II
    Theodor Agapie; Fiscal Year: 2013
    ....
  7. ASSEMBLY OF CAB POLYPEPTIDES
    Neil Hoffman; Fiscal Year: 1993
    ..The aforementioned approaches should help us understand how to account for the nonrandom distribution of proteins within physically continuous membranes...
  8. CHARACTERIZATION OF MEMBRANE PROTEIN COMPLEXES
    L Staehelin; Fiscal Year: 1993
    ..Preliminary experiments show that this gel system has great potential for analyzing photosystem I-defective mutants in Chlamydomonas...
  9. International Conference on Biological Inorganic Chemistry
    Dimitri Coucouvanis; Fiscal Year: 2005
    ..A major use of the support sought in this application will assist mainly the junior participants of the meeting and particularly women and minorities among them. ..
  10. ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER
    James McCusker; Fiscal Year: 1999
    ..We believe that this investment on the part of the Institute will greatly enhance not only the research of the major user group, but of the Department of Chemistry at UC Berkeley as a whole. ..
  11. MOLECULAR ORGANIZATION OF A MEMBRANE PROTEIN COMPLEX
    Richard Sayre; Fiscal Year: 1990
    ..We propose that this research will have broad application in many membrane protein systems...
  12. GENETICS AND BIOCHEMISTRY OF A MEMBRANE PROTEIN COMPLEX
    HIMADRI PAKRASI; Fiscal Year: 1999
    ..These studies will also provide valuable insights about proteolytic degradation and subsequent repair of membrane bound proteins, key processes related to a number of human diseases as well as aging. ..
  13. APPLICATIONS OF A RESONANCE RAMAN SPECTROMETER
    Russ Hille; Fiscal Year: 1991
    ..The proposed research constitutes essentially complete utilization in terms of both time and capabilities of a modern, versatile resonance Raman spectrometer...
  14. High-Valent Metal Oxos to Model OEC in Photosystem II
    THEODORE BETLEY; Fiscal Year: 2007
    ..The models described herein are unique in that they will facilitate both acid-base and radical- coupling O-O bond forming reaction mechanisms to be investigated. [unreadable] [unreadable]..
  15. METAL-DIOXYGEN CHEMISTRY OF BIOLOGICAL INTEREST
    William Armstrong; Fiscal Year: 1991
    ....
  16. MEMBRANE PROTEIN STRUCTURE : USE OF LIPIDIC MESOPHASES
    Martin Caffrey; Fiscal Year: 2004
    ..The results of these studies, together with an analysis in detail of the crystal/lipid interface will then be used in attempts at rational crystallization of the previously uncrystallized photosystem II from Chlamydomonas. ..
  17. SPECTROSCOPIC STUDIES OF LIGHT-DRIVEN ELECTRON TRANSFER
    BRIDGETTE ANNE BARRY; Fiscal Year: 2010
    ..This specific aim will serve to elucidate the role of primary, secondary, and tertiary interactions in a structurally defined, tractable model system. ..
  18. PULSED EPR STUDIES OF BIOLOGICAL MANGANESE CLUSTERS
    R David Britt; Fiscal Year: 2010
    ....
  19. FTIR Studies of Photosynthetic Oxygen Evolution
    RICHARD DEBUS; Fiscal Year: 2009
    ..Its advantages derive from its ability to be stepped through its catalytic cycle with single flashes of light, thereby facilitating kinetic studies of reaction cycle intermediates with high time resolution. ..
  20. Biophysical and Bioinorganic Studies of Oxygen Evolution
    Gary W Brudvig; Fiscal Year: 2010
    ....
  21. DISTANCE MEASUREMENTS IN METALLOPROTEINS
    GARY BRUDVIG; Fiscal Year: 2002
    ....
  22. EPR & Optical Studies of Photosynthetic Water Oxidation
    RICHARD DEBUS; Fiscal Year: 2005
    ..abstract_text> ..
  23. EPR Studies of Biological PCET Elements
    R Britt; Fiscal Year: 2008
    ....
  24. EPR STUDIES OF METAL/NUCLEI ACID INTERACTIONS
    R Britt; Fiscal Year: 2003
    ..abstract_text> ..
  25. Structure of the Survival Motor Neuron Protein (SMN)
    James Allen; Fiscal Year: 2006
    ..Together, these results should enhance our understanding of the molecular reasons for SMA and potentially lead to new strategies for the development of therapies. ..
  26. STRUCTURE AND FUNCTION OF CAROTENOIDS
    Harry Frank; Fiscal Year: 2008
    ..Several preliminary studies have already been carried out by us in support of the methods we propose to employ. ..
  27. Reverse genetics of Chlamydomonas using TILLING
    Krishna Niyogi; Fiscal Year: 2008
    ..This project will generate a resource that will enable the determination of gene function for many genes that have been discovered by sequencing the Chlamydomonas genome. [unreadable] [unreadable] [unreadable]..
  28. Minimized Streptokinase
    William Coleman; Fiscal Year: 2002
    ..Successful modification of streptokinase will also provide a model for engineering other therapeutic proteins. ..
  29. Assembly and Functions of Photosynthetic Water Oxidases
    G Dismukes; Fiscal Year: 2006
    ..abstract_text> ..
  30. Nutritional Copper Signaling and Homeostasis
    Sabeeha Merchant; Fiscal Year: 2010
    ..Cu cells and err I vs. wild-type cells, in parallel with sub-proteomic gel- and multi-dimensional chromatography-based analyses of copper-replete vs. copper-deficient cells. ..
  31. STRUCTURAL INVESTIGATIONS OF METALLOPROTEIN METAL SITES
    James Penner Hahn; Fiscal Year: 2008
    ....
  32. ANTIOXIDANT METABOLISM IN CHLAMYDOMONAS
    Krishna Niyogi; Fiscal Year: 2008
    ..abstract_text> ..
  33. FREE RADICALS IN BIOLOGICAL REDOX PROCESSES
    John McCracken; Fiscal Year: 2002
    ....
  34. MECHANISM OF OXIDATIVE DNA DAMAGE IN MODEL ORGANISMS
    James Imlay; Fiscal Year: 2002
    ..Further, by understanding how metabolic perturbations affect DNA oxidation, we may be able to anticipate the affects of drug therapies upon mutagenesis and cell death. ..
  35. STRUCTURE AND REACTIVITY STUDIES OF MANGANESE ENZYMES
    James Penner Hahn; Fiscal Year: 2003
    ..The proposed experiments will allow more complete comparison between Fe and Mn sites in these proteins. ..
  36. Inhibitor-Resistant Thermostable DNA Polymerases for PCR
    William Coleman; Fiscal Year: 2004
    ..By creating application-specific polymerase variants engineered to resist inhibitors, this technology will provide PCR users with cost-effective and time-saving reagents that will improve the reliability of their assays. ..
  37. HemeCu Oxidase: Calculated Electron and Proton Transfers
    MARILYN GUNNER; Fiscal Year: 2005
    ..The effects of mutation will be predicted. Protein structural motifs that stabilize charge transfers deep inside membrane proteins and couple these electron transfers to transmembrane proton transfer will be identified. ..
  38. MATURATION OF CHLOROPLAST CYTOCHROMES
    Sabeeha Merchant; Fiscal Year: 2001
    ....
  39. ADVANCED EPR STUDIES OF RADICALS IN BIOLOGICAL CATALYSIS
    John McCracken; Fiscal Year: 2006
    ..The Fet3p enzyme is a multicopper oxidase that shows ferroxidase activity and thus, shares a common reactivity with ceruloplasrnin, a mammalian rnulticopper ferroxidase that is vital to metal ion homeostasis. ..
  40. 94 GHZ/9 GHZ CONTINUOUS WAVE AND PULSED EPR SPECTROMETER
    John McCracken; Fiscal Year: 2001
    ..Purchase of an E-680 spectrometer will shift our development efforts away from hardware building and towards solving biomedical problems. ..
  41. The Physiology of Oxidative Stress in Escherichia coli
    James Imlay; Fiscal Year: 2006
    ..coli will provide a blueprint for efforts to solve key issues in oxidative stress: obligate anaerobiosis, the killing mechanism of) hagocytes, and endogenous oxidative stress in higher organisms. ..
  42. Proton-coupled electron translocation in metalloenzymes
    ROBERT CUKIER; Fiscal Year: 2006
    ..Contemporaneously, we will explore PTR by simulation methodologies that we are developing, which combine quantum mechanics and molecular dynamics to quantitatively describe proton translocation. ..
  43. HIGH THROUGHPUT CHARACTERIZATION OF THE YEAST METALLOME
    James Penner Hahn; Fiscal Year: 2007
    ..abstract_text> ..
  44. Singlet Oxygen Signaling
    Krishna Niyogi; Fiscal Year: 2007
    ..This investigation will help illuminate studies of singlet oxygen biology in other organisms and provide fundamental knowledge about how eukaryotic cells sense and respond to singlet oxygen. ..
  45. Understanding coupled transfers of electrons and protons relevant to biological c
    James Mayer; Fiscal Year: 2007
    ..abstract_text> ..