microbiological techniques

Summary

Summary: Techniques used in microbiology.

Top Publications

  1. ncbi [Optimizing microbiological controls of corneal organ culture media]
    G Thuret
    Service d Ophtalmologie, CHRU de Bellevue, Saint Etienne
    J Fr Ophtalmol 26:792-800. 2003
  2. ncbi [Air microbial sampling: the state of the art]
    Cesira Pasquarella
    Dipartimento di Sanita Pubblica, Sezione di Igiene, Universita degli Studi di Parma, Italy
    Ig Sanita Pubbl 64:79-120. 2008
  3. ncbi Diagnostic methods current best practices and guidelines for identification of difficult-to-culture pathogens in infective endocarditis
    Pierre Houpikian
    Unite des Rickettsies, CNRS UPRES A 6020 Faculté de Médecine de Marseille, 27 Boulevard Jean Moulin, 13385 Marseille, France
    Infect Dis Clin North Am 16:377-92, x. 2002
  4. ncbi ATP as a biomarker of viable microorganisms in clean-room facilities
    Kasthuri Venkateswaran
    Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Mail Stop 89 4800, Oak Grove Dr, Pasadena 91109, USA
    J Microbiol Methods 52:367-77. 2003
  5. ncbi Relationship between sampling duration and concentration of culturable airborne mould and bacteria on selected culture media
    D R Godish
    Department of Physiology and Health Science, Ball State University, Muncie, IN 47306, USA
    J Appl Microbiol 102:1479-84. 2007
  6. ncbi Ibis T5000: a universal biosensor approach for microbiology
    David J Ecker
    Ibis Biosciences, 1896 Rutherford Road, Carlsbad, California 92008, USA
    Nat Rev Microbiol 6:553-8. 2008
  7. pmc Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis
    Ephraim L Tsalik
    Department of Medicine, Division of Infectious Diseases, Duke University School of Medicine, Durham, North Carolina, USA
    J Clin Microbiol 48:26-33. 2010
  8. ncbi Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA
    Clin Chem 57:1057-67. 2011
  9. pmc Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia
    F Lamoth
    Infectious Diseases Service, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    J Clin Microbiol 48:3510-6. 2010
  10. pmc Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time
    Philippe R S Lagacé-Wiens
    Department of Medical Microbiology and Infectious Diseases, University of Manitoba Winnipeg, Manitoba, Canada
    J Clin Microbiol 50:3324-8. 2012

Detail Information

Publications256 found, 100 shown here

  1. ncbi [Optimizing microbiological controls of corneal organ culture media]
    G Thuret
    Service d Ophtalmologie, CHRU de Bellevue, Saint Etienne
    J Fr Ophtalmol 26:792-800. 2003
    ..To compare the efficiency of an automated method using blood bottles with conventional microbiological tests for controlling sterility in cornea organ culture media...
  2. ncbi [Air microbial sampling: the state of the art]
    Cesira Pasquarella
    Dipartimento di Sanita Pubblica, Sezione di Igiene, Universita degli Studi di Parma, Italy
    Ig Sanita Pubbl 64:79-120. 2008
    ....
  3. ncbi Diagnostic methods current best practices and guidelines for identification of difficult-to-culture pathogens in infective endocarditis
    Pierre Houpikian
    Unite des Rickettsies, CNRS UPRES A 6020 Faculté de Médecine de Marseille, 27 Boulevard Jean Moulin, 13385 Marseille, France
    Infect Dis Clin North Am 16:377-92, x. 2002
    ..Recently, universal and species-specific primers have been designated to amplify bacterial DNA directly from resected valves, allowing positive identification...
  4. ncbi ATP as a biomarker of viable microorganisms in clean-room facilities
    Kasthuri Venkateswaran
    Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Mail Stop 89 4800, Oak Grove Dr, Pasadena 91109, USA
    J Microbiol Methods 52:367-77. 2003
    ..When the detection of nonviable microbes is of interest, the ATP assay can be supplemented with DNA analysis...
  5. ncbi Relationship between sampling duration and concentration of culturable airborne mould and bacteria on selected culture media
    D R Godish
    Department of Physiology and Health Science, Ball State University, Muncie, IN 47306, USA
    J Appl Microbiol 102:1479-84. 2007
    ..This study was designed to evaluate potential effects of sampling duration on observed concentrations of airborne culturable mould and bacteria on selected media...
  6. ncbi Ibis T5000: a universal biosensor approach for microbiology
    David J Ecker
    Ibis Biosciences, 1896 Rutherford Road, Carlsbad, California 92008, USA
    Nat Rev Microbiol 6:553-8. 2008
    ....
  7. pmc Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis
    Ephraim L Tsalik
    Department of Medicine, Division of Infectious Diseases, Duke University School of Medicine, Durham, North Carolina, USA
    J Clin Microbiol 48:26-33. 2010
    ..Current microbiological techniques are limited in their diagnostic capacities and timeliness...
  8. ncbi Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA
    Clin Chem 57:1057-67. 2011
    ..A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples...
  9. pmc Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia
    F Lamoth
    Infectious Diseases Service, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    J Clin Microbiol 48:3510-6. 2010
    ..Technical adjustments may enhance the efficiency of this new molecular tool in this specific setting...
  10. pmc Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time
    Philippe R S Lagacé-Wiens
    Department of Medical Microbiology and Infectious Diseases, University of Manitoba Winnipeg, Manitoba, Canada
    J Clin Microbiol 50:3324-8. 2012
    ..However, the use of MALDI-TOF for direct identification is accurate and should result in reduced turnaround time to identification...
  11. ncbi Use of fluorescence spectroscopy to differentiate yeast and bacterial cells
    Hemant Bhatta
    Department of Physics, Division of Information and Communication Sciences, Macquarie University, North Ryde, NSW 2109, Australia
    Appl Microbiol Biotechnol 71:121-6. 2006
    ....
  12. pmc Multiplex PCR for rapid and improved diagnosis of bloodstream infections in liver transplant recipients
    Peter Michael Rath
    Institute of Medical Microbiology, University Hospital Essen, University of Duisburg Essen, Essen, Germany
    J Clin Microbiol 50:2069-71. 2012
    ..SF yielded a significantly higher positivity rate in the LTX group (52.3%) than in the non-LTX group (30.5%; P = 0.0009). SF may be a powerful tool for the early diagnosis of bloodstream infections in LTX patients...
  13. pmc Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis
    Barbara Lucignano
    Unit of Microbiology, Children s Hospital and Research Institute Bambino Gesu, Rome, Italy
    J Clin Microbiol 49:2252-8. 2011
    ..8%] versus n = 26 [1.6%]), respectively; P < 0.0001). SeptiFast served as a highly valuable adjunct to conventional blood culture in children, adding diagnostic value and shortening the time to result (TTR) to 6 h...
  14. pmc Species divergence and the measurement of microbial diversity
    Catherine A Lozupone
    Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
    FEMS Microbiol Rev 32:557-78. 2008
    ..Consequently, divergence-based methods are providing new insights into microbial community structure and function...
  15. pmc Comparative analysis of human gut microbiota by barcoded pyrosequencing
    Anders F Andersson
    Swedish Institute for Infectious Disease Control, Solna, Sweden
    PLoS ONE 3:e2836. 2008
    ..Here we applied the technique to analyze microbial communities in throat, stomach and fecal samples. Our results demonstrate the applicability of barcoded pyrosequencing as a high-throughput method for comparative microbial ecology...
  16. ncbi Comparative evaluation of the BACTEC MGIT 960 system with solid medium for isolation of mycobacteria
    J J Lee
    Department of Internal Medicine, Buddhist Tzu Chi General Hospital, Hualien, Hualien County, Taiwan
    Int J Tuberc Lung Dis 7:569-74. 2003
    ..The utilisation of new, rapid methods of diagnosis of tuberculosis is currently of great interest for tuberculosis control. This study was carried out in a teaching hospital in the eastern region of Taiwan...
  17. ncbi Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood
    F Wallet
    Pole de Microbiologie, Centre Hospitalier Universitaire de Lille, Faculte de Medicine de Lille, France
    Clin Microbiol Infect 16:774-9. 2010
    ..Our results suggest that the LC-SF test may be a valuable complementary tool in the management of patients with clinically suspected sepsis...
  18. ncbi Determination of folate vitamers in human serum by stable-isotope-dilution tandem mass spectrometry and comparison with radioassay and microbiologic assay
    Christine M Pfeiffer
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Clin Chem 50:423-32. 2004
    ....
  19. ncbi Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis
    Danièle Maubon
    Infectious Agent Department, Parasitology Mycology Laboratory, Albert Michallon Teaching Hospital, 38043 Grenoble Cedex 9, France
    J Infect 61:335-42. 2010
    ..We evaluated the therapeutic usefulness of multiplex PCR testing in patients with cancer and sepsis...
  20. ncbi Reducing the global burden of tuberculosis: the contribution of improved diagnostics
    Emmett Keeler
    RAND Corporation, 1776 Main Street, PO Box 2138, Santa Monica, California 90407 2138, USA
    Nature 444:49-57. 2006
  21. pmc Determination of unmetabolized folic acid in human plasma using affinity HPLC
    Renee Kalmbach
    Vitamin Metabolism and Aging Laboratory, Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
    Am J Clin Nutr 94:343S-347S. 2011
    ..Folic acid (FA) fortification of food created the need to determine whether fortification elevated concentrations of unmetabolized FA in plasma and whether this form of the vitamin in blood is associated with adverse health outcomes...
  22. pmc Folate status assessment history: implications for measurement of biomarkers in NHANES
    Barry Shane
    Department of Nutritional Science and Toxicology, University of California, Berkeley, CA 94720 3104, USA
    Am J Clin Nutr 94:337S-342S. 2011
    ..Past experience with microbiological and competitive protein binding assays indicates some of the technical problems that research will need to address before this promise becomes reality...
  23. ncbi Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices
    Karin Martin
    Hans Knöll Institute for Natural Products Research, Jena, Germany
    Lab Chip 3:202-7. 2003
    ..Rapid growth of the microcultures was observed. Cell densities were found to be as high as in conventional shake flask cultures...
  24. ncbi Combination of analytical and microbiological techniques to study the antimicrobial activity of a new active food packaging containing cinnamon or oregano against E. coli and S. aureus
    R Becerril
    Lab Microbiología, Facultad de Medicina, Universidad de Zaragoza, C Domingo Miral s n, Zaragoza, Spain
    Anal Bioanal Chem 388:1003-11. 2007
    The aim of this work is the optimization and application of a group of analytical and microbiological techniques in the study of the activity of essential oils (EOs) incorporated in a new antimicrobial packaging material and the research ..
  25. ncbi The future of single-cell environmental microbiology
    Marcel M M Kuypers
    Max Planck Institute for Marine Microbiology, Bremen, Germany
    Environ Microbiol 9:6-7. 2007
  26. ncbi Influence of 5,10-methylenetetrahydrofolate reductase polymorphism on whole-blood folate concentrations measured by LC-MS/MS, microbiologic assay, and bio-rad radioassay
    Zia Fazili
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30345, USA
    Clin Chem 54:197-201. 2008
    ....
  27. ncbi Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts
    Tadayuki Tsukatani
    Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume 839 0861, Japan
    J Microbiol Methods 75:109-16. 2008
    ..Linear relationships between the absorbance and viable microbial cell density were obtained in all microorganisms, suggesting that the absorbance change reflected the microbial cell proliferation...
  28. ncbi Evaluation of impression smear in the diagnosis of fungal keratitis
    Arun K Jain
    Department of Ophthalmology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
    Indian J Ophthalmol 55:33-6. 2007
    ..To evaluate and compare impression smear with the conventional mechanical corneal scraping by potassium hydroxide (KOH) method in the diagnosis of fungal keratitis...
  29. ncbi Xanthan gum: an economical partial substitute for agar in microbial culture media
    Shashi B Babbar
    Department of Botany, University of Delhi, Delhi, India
    Curr Microbiol 52:287-92. 2006
    ..Likewise, differential medium, CRMA (Congo red mannitol agar) gelled with xanthan-agar combination could differentiate between Agrobacterium tumefaciens and Rhizobium sp...
  30. pmc Controlled clinical comparison of plastic and glass bottles of BacT/ALERT FA medium for culturing organisms from blood of adult patients
    Cathy A Petti
    Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina, USA
    J Clin Microbiol 43:1960-2. 2005
    ..We conclude that the BacT/ALERT plastic and glass FA bottles are comparable for recovery of microorganisms and that the safety advantage of plastic bottles can be achieved without compromising performance...
  31. ncbi Guar gum: a cheap substitute for agar in microbial culture media
    R Jain
    Department of Botany, University of Delhi, Delhi, India
    Lett Appl Microbiol 41:345-9. 2005
    ..To determine the possibility of using guar gum, a colloidal polysaccharide, as a cheap alternative to agar for gelling microbial culture media...
  32. pmc Comparative recovery of microorganisms from BacT/ALERT plastic and glass FA and FN blood culture bottles
    J A Riley
    Division of Laboratory Medicine, Geisinger Medical Center, Danville, PA 17822 0131, USA
    J Clin Microbiol 43:3244-6. 2005
    ..02). These data suggest that recovery of microorganisms with plastic FA/FN bottles is at least equal to that with glass FA/FN bottles while offering greater safety for users...
  33. pmc Persistence of pigment production by yeast isolates grown on CHROMagar Candida medium
    Duane R Hospenthal
    Department of Medicine, Brooke Army Medical Center, Fort Sam Houston, Texas, USA
    J Clin Microbiol 40:4768-70. 2002
    ..Candida tropicalis produced colonies similar to those of rare Cryptococcus and Trichosporon species, and mixed cultures were often difficult to identify as such...
  34. pmc Controlled clinical comparison of the BacT/ALERT FN and the standard anaerobic SN blood culture medium
    S Mirrett
    Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Microbiol 42:4581-5. 2004
    ..We conclude that the companion anaerobic FN bottle detects more microorganisms than does the anaerobic SN bottle when used in conjunction with the nonvented aerobic FA bottle in the BacT/ALERT blood culture system...
  35. ncbi Methods of studying soil microbial diversity
    Jennifer L Kirk
    Department of Environmental Biology, University of Guelph, Ontario Agricultural College, Guelph, Ontario, Canada
    J Microbiol Methods 58:169-88. 2004
    ..Soil microorganisms, such as bacteria and fungi, play central roles in soil fertility and promoting plant health. This review examines and compares the various methods used to study microbial diversity in soil...
  36. ncbi Enumeration of airborne bacteria and fungi using solid phase cytometry
    Lies M E Vanhee
    Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, Ghent, Belgium
    J Microbiol Methods 72:12-9. 2008
    ..Results show that SPC can be considered superior to the culture-based method because of its much higher dynamic range, its speed and its ability to enumerate not only culturable but all viable micro-organisms...
  37. ncbi Ultrafiltration-based techniques for rapid and simultaneous concentration of multiple microbe classes from 100-L tap water samples
    Amy L Polaczyk
    University of North Carolina at Charlotte, USA
    J Microbiol Methods 73:92-9. 2008
    ....
  38. ncbi Factors affecting the outcome of the acidification power test of yeast quality: critical reappraisal
    K Sigler
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia
    Folia Microbiol (Praha) 51:525-34. 2006
    ..Since an exact evaluation of acidification characteristics depends strongly on the kinetics of the process, the AP test should include monitoring the time course of the acidification...
  39. ncbi Evaluation of combined deactivators-supplemented agar medium (CDSAM) for recovery of dermatophytes from patients with tinea pedis
    Makoto Adachi
    Department of Dermatology, Teikyo University School of Medicine, Itabashi, Tokyo, Japan
    Med Mycol 45:347-9. 2007
    ....
  40. ncbi New ethacridine derivatives as the potential antifungal and antibacterial preparations
    Vilma Petrikaite
    Department of Pharmaceutical Chemistry and Pharmacognosy, Kaunas University of Medicine, Kaunas, Lithuania
    Medicina (Kaunas) 43:657-63. 2007
    ..The obtained results gave the opportunity to separate the perspective group of potential antiinfective compounds...
  41. ncbi Culture clash: challenging the dogma of microbial diversity
    Stuart P Donachie
    Department of Microbiology, University of Hawai i, Honolulu, HI, USA
    ISME J 1:97-9. 2007
  42. ncbi An efficient method to obtain axenic cultures of Alexandrium tamarense--a PSP-producing dinoflagellate
    Jianqiang Su
    School of Life Sciences, MOE of Key Laboratory for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen, Fujian 361005, China
    J Microbiol Methods 69:425-30. 2007
    ..This method is more efficient and less time-consuming than other methods and the resultant axenic A. tamarense cultures would provide a simpler system for further study of bacteria-alga interactions...
  43. ncbi Microbiologic findings of sinusitis by a novel method for obtaining culture
    Murat Yariktas
    Department of Otorhinolaryngology, Suleyman Demirel University School of Medicine, Isparta, 32260 Turkey
    Diagn Microbiol Infect Dis 58:49-52. 2007
    ..0296). During ESS, the identification of the pathogen in sinusitis with endoscopically guided narrow-bore sinus culture applicator is a reliable diagnostic method compared with the classic culture techniques...
  44. ncbi A "bucket of light" for viewing bacterial colonies in soft agar
    John S Parkinson
    Department of Biology, University of Utah, Salt Lake City, UT, USA
    Methods Enzymol 423:432-5. 2007
    ..This brief chapter describes a simple and relatively inexpensive illumination device for viewing and photographing bacterial colonies in soft agar...
  45. ncbi Diagnostic methods current best practices and guidelines for isolation of bacteria and fungi in infective endocarditis
    Michael L Towns
    BD Diagnostic Systems, 7 Loveton Circle, Sparks, MD 21152, USA
    Infect Dis Clin North Am 16:363-76, ix-x. 2002
    ..Finally, "best practices" recommendations are presented to optimize the likelihood of reaching a correct etiologic diagnosis while minimizing the number of "culture-negative" cases...
  46. ncbi Rapid differentiation between fluconazole-sensitive and -resistant species of Candida directly from positive blood-culture bottles by real-time PCR
    L Metwally
    Department of Medical Microbiology, Royal Victoria Hospital, Belfast, Northern Ireland
    J Med Microbiol 56:964-70. 2007
    ..glabrata and C. krusei in comparison with a conventional phenotypic method, from approximately 72 to <3 h, and consequently allows optimization of the antifungal regimen at an earlier stage...
  47. ncbi Evaluation of peptone glucose fluconazole agar as a selective medium for rapid and enhanced isolation of Aspergillus fumigatus from the respiratory tract of bronchopulmonary aspergillosis patients colonized by Candida albicans
    H S Randhawa
    Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
    Med Mycol 44:343-8. 2006
    ..fumigatus from sputum of patients clinically suspected of aspergillosis with C. albicans colonization in their respiratory tract...
  48. ncbi Comparison of M4 and M4RT media for transporting cervical swab samples for PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae
    Jaber Aslanzadeh
    Department of Laboratory Medicine, University of Connecticut Health Center, Farmington 06030, USA
    Ann Clin Lab Sci 32:61-4. 2002
    ..M4RT medium is more convenient to use, as it did not require refrigeration until it was inoculated with the clinical sample...
  49. ncbi Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances
    Hisatoshi Kaneko
    Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, 960 1295, Japan
    J Biochem Biophys Methods 70:499-501. 2007
    ..The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted...
  50. ncbi Applicability of a rapid duplex real-time PCR assay for speciation of Campylobacter jejuni and Campylobacter coli directly from culture plates
    Emma L Best
    Campylobacter Reference Unit, Laboratory of Enteric Pathogens, Specialist and Reference Microbiology Division, Health Protection Agency, 61, London, NW9 5HT, UK
    FEMS Microbiol Lett 229:237-41. 2003
    ..6% being identified as either C. jejuni or C. coli by this technique. Rapidity, combined with specificity and sensitivity, makes this method for routine campylobacter speciation attractive to any laboratory with a Taqman system...
  51. ncbi Genomic DNA isolation from different biological materials
    Duckchul Park
    Ecological Genetics Laboratory, Landcare Research Ltd, Auckland, New Zealand
    Methods Mol Biol 353:3-13. 2007
    ..g., PCR or genomic DNA library-cloning quality)...
  52. ncbi A preliminary evaluation of a new selective agar supplemented with desferrioxamine for detection of methicillin-resistant Staphylococcus aureus
    T Monsen
    Department of Clinical Bacteriology, University Hospital of Umea, S 901 85 Umea, Sweden
    J Hosp Infect 54:216-21. 2003
    ..The detection rate was higher for agars supplemented with methicillin than for agars supplemented with oxacillin...
  53. ncbi Collection of preputial material by scraping and aspiration for the diagnosis of Tritrichomonas foetus in bulls
    P C Irons
    Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa
    J S Afr Vet Assoc 73:66-9. 2002
    ..It is concluded that preputial scraping is as effective as washing and represents a suitable alternative for the collection of material for direct examination and culture of Tritrichomonas foetus...
  54. ncbi Debaryomyces hansenii growth in nonsterile seawater ClO2-peptone-containing medium
    M Ramírez-Orozco
    Centre for Biological Research, Centro de Investigaciones Biologicas del Noroeste, La Paz B C S, Mexico
    Can J Microbiol 47:676-9. 2001
    ..Because the concentration of ClO2 decreases during the fermentation, we ascribe to D. hansenii cells a "biocontrol" action that contributes to the collection of a contaminant-free yeast cell biomass...
  55. ncbi Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body fluids
    Emel Sesli Cetin
    Department of Microbiology and Clinical Microbiology, Suleyman Demirel University Medical Faculty, Isparta, Turkey
    Adv Ther 24:1271-7. 2007
    ....
  56. ncbi What's the 'meta' with metagenomics?
    Grieg F Steward
    Department of Oceanography, University of Hawaii at Manoa, Honolulu, HI, USA
    ISME J 1:100-2. 2007
  57. ncbi Evaluation of redox indicators and the use of digital scanners and spectrophotometer for quantification of microbial growth in microplates
    Jenny Gabrielson
    Microbiology and Tumorbiology Center, Karolinska Institutet, Box 280, 171 77 Stockholm, Sweden
    J Microbiol Methods 50:63-73. 2002
    ..Both the XTT and INT showed lower correlations. It is concluded that bacterial growth/growth inhibition can be easily and reproducibly measured from microplate cultivations with a flatbed scanner or with a microplate spectrophotometer...
  58. ncbi Effect of culture media temperature on bacterial recovery from corneal scrapings
    Kirk R Wilhelmus
    Insight 28:82-3. 2003
  59. ncbi The application of chromogenic media in clinical microbiology
    J D Perry
    Microbiology Department, Freeman Hospital, Newcastle upon Tyne, UK
    J Appl Microbiol 103:2046-55. 2007
    ..The purpose of this review is to provide some insight into how such media work and appraise their utility in routine clinical diagnostics, in comparison with conventional media...
  60. ncbi INDISIM, an individual-based discrete simulation model to study bacterial cultures
    Marta Ginovart
    Escola Superior d Agricultura de Barcelona EUETAB CEIB, Universitat Politecnica de Catalunya, Urgell 187, 08036 Barcelona, Spain
    J Theor Biol 214:305-19. 2002
    ..The simulation results are found to be in very good qualitative agreement with available experimental data, and provide useful insights into the mechanisms involved in each case...
  61. ncbi A simple agar plate assay for screening siderophore producer yeasts
    V Calvente
    , , , , Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina
    J Microbiol Methods 47:273-9. 2001
    ..The methodology was also a useful tool for making a preliminary assessment of the influence of different factors on the siderophore production...
  62. ncbi The Moore's Law of microbiology - towards bacterial culture miniaturization with the micro-Petri chip
    Orit Gefen
    Racah Institute of Physics and the Center for Nanoscience and Nanotechnology, Hebrew University, Givat Ram, Jerusalem 91904, Israel
    Trends Biotechnol 26:345-7. 2008
    ....
  63. ncbi Comparison of direct selective versus nonselective agar media plus LIM broth enrichment for determination of group B streptococcus colonization status in pregnant women
    Sameer Elsayed
    Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta
    Arch Pathol Lab Med 127:718-20. 2003
    ....
  64. pmc Clinical benefit of recovering dermatophytes from skin swabs sent for bacterial culture
    Gillian Mabbott
    Department of Microbiology, Diagnostic Medlab, Panmure, Auckland, New Zealand
    J Clin Microbiol 42:4838-9. 2004
    ..3%) of 22,613 cultures. Twenty-one patients received specific antifungal treatment when their dermatophyte was reported. Most clinicians thought recovering and reporting the dermatophyte contributed to patient management...
  65. ncbi Automated agar plate streaker: a linear plater on Society for Biomolecular Sciences standard plates
    Gregory W King
    Department of Research Operations, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biomol Screen 11:704-11. 2006
    ..This novel instrument is capable of dispensing discrete volumes of sample in a linear pattern. It can be programmed to dispense a sample suspense at a uniform application rate or across a decreasing concentration gradient...
  66. ncbi Microbial gene expression in soil: methods, applications and challenges
    Saleema Saleh-Lakha
    Department of Environmental Biology, University of Guelph, Guelph, ON, Canada N1G 2W1
    J Microbiol Methods 63:1-19. 2005
    ..This review will also address information gaps in bacterial gene expression in soil and possible future research to develop an understanding of microbial activities in soil environments...
  67. ncbi Genotypic microbial community profiling: a critical technical review
    Andreas Nocker
    Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717 3980, USA
    Microb Ecol 54:276-89. 2007
    ..In this document, we present and critically compare these methods commonly used for the study of microbial diversity...
  68. ncbi New insights into polylactide biodegradation from molecular ecological techniques
    Parveen Sangwan
    CSIRO Materials Science and Engineering, Victoria 3168, Australia
    Macromol Biosci 8:304-15. 2008
    ....
  69. ncbi [Molecular biology-based methods for pathogen detection in endophthalmitis]
    S Zimmermann
    Abteilung für Hygiene und Medizinische Mikrobiologie, Hygiene Institut des Universitätsklinikums Heidelberg, Heidelberg, Deutschland
    Ophthalmologe 104:940-6. 2007
    ..In immunocompromised patients molecular techniques show more accurate results than serological ones. However, molecular diagnostic tools also bear limitations that have to be considered...
  70. ncbi PNA FISH: an intelligent stain for rapid diagnosis of infectious diseases
    Henrik Stender
    AdvanDx, Inc, 25K Olympia Avenue, Woburn, MA 01801, USA
    Expert Rev Mol Diagn 3:649-55. 2003
    ..Having transitioned from an academic curiosity into an advanced diagnostic tool, PNA probes are now debuting on the infectious disease stage, representing the new generation of therapy-directing diagnostics...
  71. ncbi Cell differentiation and colony alteration of an edible terrestrial cyanobacterium Nostoc flagelliforme, in liquid suspension cultures
    X J Liu
    Department of Botany, University of Hong Kong, Pokfulam Road, Hong Kong, China
    Folia Microbiol (Praha) 48:619-26. 2003
    ..The new information on the growth and morphology of N. flagelliforme could be potentially used for the scale-up or field cultivation...
  72. ncbi Using agar plates to teach the importance of hand hygiene
    Michael Bauer
    School of Nursing and Midwifery, La Trobe University, Bundoora, Victoria, Australia
    Nurse Educ 32:236-7. 2007
  73. ncbi Effect of sampling time on the culturability of airborne fungi and bacteria sampled by filtration
    Kate T H Durand
    University of Washington, School of Public Health and Community Medicine, Department of Environmental Health, Seattle 98195, USA
    Ann Occup Hyg 46:113-8. 2002
    ..This suggests that increased sampling time does not affect the viability of the organisms commonly found in the air at composting facilities...
  74. ncbi Rapid diagnosis in pediatric infectious diseases: the past, the present and the future
    Michael D Nissen
    Department of Infectious Diseases, Royal Children s Hospital Brisbane, Herston, Australia
    Pediatr Infect Dis J 21:605-12; discussion 613-4. 2002
    ..The completion of the genome sequencing projects for significant microbial pathogens, in combination with PCR and DNA chip technology, will revolutionize the diagnosis and management of infectious diseases...
  75. ncbi Implications of new technology for infectious diseases practice
    Ellen Jo Baron
    Department of Pathology, Division of Infectious Diseases and Geographic Medicine, Stanford University Medical Center, Stanford, CA 94305, USA
    Clin Infect Dis 43:1318-23. 2006
    ....
  76. ncbi High-throughput culturing of fungi from plant litter by a dilution-to-extinction technique
    Javier Collado
    Centro de Investigación Básica, Merck Sharp and Dohme de Espana, S A, Madrid, Spain
    FEMS Microbiol Ecol 60:521-33. 2007
    ..Intercolony interactions are reduced, permitting longer incubation times, and colony initiation and recovery improved. Effort to evaluate and recover colonies from fungal isolation plates was substantially reduced...
  77. ncbi Application of elements of microbiological risk assessment in the food industry via a tiered approach
    Suzanne J C van Gerwen
    Unilever Research and Development, Foods Research Centre, 3130 AC, Vlaardingen, The Netherlands
    J Food Prot 67:2033-40. 2004
    ..The tiered prototype is a tool to be used by experienced microbiologists as a basis for advice to product developers and can help to make safety assurance for new food concepts transparent to food inspection services...
  78. ncbi Detection of airborne microbes in a composting facility by cultivation based and cultivation-independent methods
    Andreas Albrecht
    Federal Institut for Work Safety and Health, Berlin, Germany
    Ann Agric Environ Med 14:81-5. 2007
    ..Consequently, a risk assessment based only on measuring colony forming units may, in some cases, not be sufficient...
  79. ncbi Characterisation of prototype Nurmi cultures using culture-based microbiological techniques and PCR-DGGE
    Sinead M Waters
    Alltech Ireland, Sarney, Summerhill Road, Dunboyne, Co Meath, Ireland
    Int J Food Microbiol 110:268-77. 2006
    ..Traditional microbiological techniques and a molecular culture-independent methodology, polymerase chain reaction combined with denaturing ..
  80. ncbi Mechanical transmission of pathogenic organisms: the role of cockroaches
    Y M Tatfeng
    Lahor Public Health and Research Centre, Benin City, Edo State, Nigeria
    J Vector Borne Dis 42:129-34. 2005
    ..Cockroaches (Diploptera punctata) are basically tropical insects and will do their best to find a home that is both warm and moist. Their involvement in the transmission of tropical diseases is poorly investigated in Africa...
  81. pmc High incidence of antimicrobial resistant organisms including extended spectrum beta-lactamase producing Enterobacteriaceae and methicillin-resistant Staphylococcus aureus in nasopharyngeal and blood isolates of HIV-infected children from Cape Town, South
    Mark F Cotton
    Department of Paediatrics, Faculty of Health Sciences, Stellenbosch University, Cape Town South Africa
    BMC Infect Dis 8:40. 2008
    ....
  82. ncbi Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases
    Helena Tlaskalova-Hogenova
    Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Immunol Lett 93:97-108. 2004
    ..More than 50% of intestinal bacteria cannot be cultured by conventional microbiological techniques. Molecular biological methods help in analysing the structural and functional complexity of the ..
  83. ncbi Development and validation of a mathematical model to describe the growth of Pseudomonas spp. in raw poultry stored under aerobic conditions
    Silvia A Dominguez
    Food Risk Analysis Initiative, Food Science Building, 65 Dudley Road, Rutgers University, New Brunswick, NJ 08901 8520, USA
    Int J Food Microbiol 120:287-95. 2007
    ..growth under aerobic conditions can provide a fast and cost-effective alternative to traditional microbiological techniques to estimate the effects of storage temperature on product shelf-life...
  84. ncbi Production, characterization, and antimicrobial activity of a bacteriocin from newly isolated Enterococcus faecium IJ-31
    Imran Javed
    Department of Microbiology, Quaid i Azam University, Islamabad 45320, Pakistan
    J Food Prot 73:44-52. 2010
    ..from indigenous dairy products in Islamabad, Pakistan. By classical microbiological techniques, one strain from a butter sample was identified to be Enterococcus faecium, and we designated it E...
  85. pmc Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis
    E Smit
    Microbiological Laboratory for Health Protection, National Institute of Public Health and the Environment RIVM, NL 3720 BA Bilthoven, The Netherlands
    Appl Environ Microbiol 65:2614-21. 1999
    ..As only a small fraction of the fungi present in soil can be cultured, conventional microbiological techniques yield only limited information on the composition and dynamics of fungal communities in soil...
  86. pmc A review of the clinical presentation of dientamoebiasis
    Damien Stark
    Department of Microbiology, St Vincent s Hospital, Darlinghurst 2010, NSW, Australia
    Am J Trop Med Hyg 82:614-9. 2010
    ..Bacterial and viral causes of infection were excluded by routine microbiological techniques. Treatment of D...
  87. pmc Phylogenetic diversity of nitrogen fixation genes in the symbiotic microbial community in the gut of diverse termites
    M Ohkuma
    The Institute of Physical and Chemical Research RIKEN and Japan Science and Technology Corporation JST, Wako, Saitama 351 0198, Japan
    Appl Environ Microbiol 65:4926-34. 1999
    ..from the termites formed lineages distinct from those previously recognized in studies using classical microbiological techniques, and several sequence clusters unique to termites were found...
  88. ncbi Inflammatory response in patients with malignant obstructive jaundice
    Mikael Ljungdahl
    Department of Surgery, University Hospital, Uppsala, Sweden
    Scand J Gastroenterol 42:94-102. 2007
    ..The aim of this study was to investigate the inflammatory and the local cellular immune response in patients accepted for surgery because of tumours in the hepatic-pancreatic-biliary (HPB) tract...
  89. ncbi Bacterial pathogens in ambulances: results of unannounced sample collection
    Donald W Alves
    Department of Emergency Medicine, Division of Special Operations, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21224, USA
    Prehosp Emerg Care 12:218-24. 2008
    ..Additionally, the nature of emergency medical services creates pressures on prehospital care providers...
  90. ncbi Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review
    Spinello Antinori
    Dipartimento di Scienze Cliniche L Sacco, Sezione di Malattie Infettive e Tropicali, Universita di Milano, Milan, Italy
    Clin Infect Dis 44:1602-10. 2007
    ..To overcome some of the limitations of conventional microbiologic techniques, polymerase chain reaction (PCR)-based assays are proposed as useful tools for the diagnosis of visceral leishmaniasis...
  91. ncbi The antimicrobial efficacy of commercial dentifrices
    Violet I Haraszthy
    Restorative Dentistry, University at Buffalo, School of Dental Medicine, New York, USA
    Gen Dent 58:50-5; quiz 56-7, 79-80. 2010
    ..b>Microbiological techniques determined the minimum inhibitory concentrations (MICs) of each dentifrice necessary to inhibit the ..
  92. ncbi Effects of prophylactic antibiotics on endometrial flora in women with postcesarean endometritis
    E R Newton
    Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio, USA
    Obstet Gynecol 92:262-8. 1998
    ..To determine the effect of prophylactic antibiotics on endometrial and endocervical microflora upon diagnosis of postcesarean endometritis...
  93. ncbi Microbiology of diabetic foot infections in a teaching hospital in Malaysia: a retrospective study of 194 cases
    Nadeem Sajjad Raja
    Department of Medical Microbiology, University of Malaya Medical Centre, Kuala Lumpur, Malaysia
    J Microbiol Immunol Infect 40:39-44. 2007
    ..This study investigated the microbiology of diabetic foot infections and their resistance to antibiotics in patients with diabetic foot infections treated at University of Malaya Medical Centre in Kuala Lumpur, Malaysia...
  94. ncbi Prevalence of Aeromonas in different clinical and water samples with special reference to gastroenteritis
    Bharat Mani Pokhrel
    Department of Microbiology, Institute of Medicine, T U Teaching Hospital, Maharajgunj, P O Box 3578, Kathmandu, Nepal
    Nepal Med Coll J 6:139-43. 2004
    ..The samples were collected and processed by standard microbiological techniques in order to isolate Aeromonas...
  95. ncbi Characteristics of brain abscess with isolation of anaerobic bacteria
    Gwenael Le Moal
    Service de Medecine Interne et Maladies Infectieuses, CHU La Miletrie, Poitiers, France
    Scand J Infect Dis 35:318-21. 2003
    ..Using appropriate microbiological techniques, 41 anaerobic bacteria strains were isolated in 22 of 42 patients (52.4%) with BA...
  96. ncbi A randomized controlled trial to assess the impact of dietary energy sources, feed supplements, and the presence of super-shedders on the detection of Escherichia coli O157:H7 in feedlot cattle using different diagnostic procedures
    Natalia Cernicchiaro
    Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
    Foodborne Pathog Dis 7:1071-81. 2010
    ..rectoanal mucosal swab (RAMS) and fecal grab samples using immunomagnetic separation (IMS) and standard microbiological techniques. On the basis of multivariable multilevel logistic regression models, we found a statistically ..
  97. ncbi Microbes in tree swallow semen
    M P Lombardo
    Department of Biology, Grand Valley State University, Allendale, Michigan 49401 9403, USA
    J Wildl Dis 36:460-8. 2000
    ..was collected directly from males after applying gentle pressure to the cloaca and we used standard microbiological techniques to identify microbes. We found that 19 of 30 samples contained one or more types of microbes...
  98. ncbi An optimized nested polymerase chain reaction (PCR) approach allows detection and characterization of human immunodeficiency virus type 1 (HIV-1) env and gag genes from clinical samples
    Dayse Locateli
    Laboratório de Imunologia Aplicada, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Florianopolis, SC, Brazil
    J Clin Lab Anal 22:106-13. 2008
    ..reaction (PCR) are of high interest due their sensitivity and specificity when compared to traditional microbiological techniques. Considering the worldwide importance of human immunodeficiency virus type 1 (HIV-1) infection, it is ..
  99. ncbi Diagnostic procedures in tularaemia with special focus on molecular and immunological techniques
    W D Splettstoesser
    Department of Medical Microbiology and Hospital Hygiene, Institute of Medical Microbiology, Virology and Hygiene, University Hospital Rostock, Schillingallee 70, 18057 Rostock, Germany
    J Vet Med B Infect Dis Vet Public Health 52:249-61. 2005
    ..Besides classical microbiological techniques (cultivation, biochemical profiling, susceptibility testing) several new immunological and molecular ..
  100. ncbi Prostatitis: what is the role of infection
    John N Krieger
    Department of Urology, School of Medicine, University of Washington, 112 UR, VA Puget Sound Health Care System, 1660 South Columbian Way, Seattle, WA 98108 1597, USA
    Int J Antimicrob Agents 19:475-9. 2002
    ..colonization/invasion of the prostate gland might occur more commonly than is appreciated by standard microbiological techniques. Specific polymerase chain reaction (PCR) assays were used for each of the pathogens previously ..
  101. ncbi Multidrug-resistant E. coli and enterobacter extraintestinal infection in 37 dogs
    J S Gibson
    School of Veterinary Science, The University of Queensland, Brisbane, QLD, Australia
    J Vet Intern Med 22:844-50. 2008
    ..Extraintestinal infections caused by multidrug-resistant (MDR) Escherichia coli and Enterobacter are becoming more common in veterinary medicine...

Research Grants54

  1. Sinusitis in Children and the Nasopharyngeal Microbiome
    Ellen Wald; Fiscal Year: 2013
    ..Finally, defining the mechanisms by which respiratory viruses cause changes in the nasopharyngeal microbiome will lead to new preventative and therapeutic measures which may be instituted long before the bacterial infection evolves. ..
  2. Control of intestinal microflora and inflammation by immune sensing of flagellin
    Janelle S Ayres; Fiscal Year: 2012
    ..bowel disease using an in vivo mouse model of colitis, mouse genetics and conventional immunological and microbiological techniques. In preliminary experiments, mouse mutants deficient for the function of two flagellin-sensing proteins ..
  3. Novel Risk Factors For Vancomycin Resistant Enterococci In Persons With Cancer
    Mini Kamboj; Fiscal Year: 2013
    ....
  4. Indicators of Recreational Water Contamination and Illness
    John M Colford; Fiscal Year: 2010
    ..Decisions about beach closures have enormous economic implications. If successful, we will quantify the relationship between health and novel rapid methods for traditional indicators as well as viral indicators. ..
  5. Human Cathepsin G: Expression, C-Terminal Processing and Dual Specificity
    David Johnson; Fiscal Year: 2007
    ..This AREA project provides students with experience in DNA cloning, microbiological techniques, cell biology, fermentation methods, protein purification, structure/function studies, enzyme/inhibitor ..
  6. Diagnostic Tool for the Point-of-Care Detection of Infection in Chronic Wounds
    MITCHELL SANDERS; Fiscal Year: 2009
    ..bacteria in chronic wounds with excellent diagnostic sensitivity and specificity relative to standard microbiological techniques. Our Phase II specific aims are focused on improvement and validation of the diagnostic test...
  7. Immunity in Early Syphillis: Pathway to HIV Coinfection
    Juan Salazar; Fiscal Year: 2007
    ..In Aim 2 we will study the effect of secondary syphilis on viral load (VL) in HIV co-infected individuals. In this aim we will compare VL between HIV patients with and without syphilis and determine the effect of penicillin on VL. ..
  8. Infection-Resistant Prosthetic Valve Sewing Cuffs
    DONALD DEMPSEY; Fiscal Year: 2004
    ..heart valve infection model, 5) assess control and Cipro-dyed Dacron sewing cuffs via histological/microbiological techniques and 6) determine physical properties of Cipro-dyed sewing cuffs post-explantation...
  9. INFECTION RESISTANT POLYURETHANE MATERIALS
    Michael Szycher; Fiscal Year: 1999
    ..The release of the antibiotic will be examined spectrophotometrically and using standard microbiological techniques. PROPOSED COMMERCIAL APPLICATION: Development of a polyurethane biomaterial with long-term infection ..
  10. Bacterial Pathogen Amplification & Real-Time Detection
    JACK U REN; Fiscal Year: 2005
    ..A single integrated system capable of isothermal amplifying and detecting all of these organisms in a closed device is proposed. ..
  11. Design/Advanced Electrostatic Sampler for Total Bioaerosols
    Gediminas Mainelis; Fiscal Year: 2007
    ..The liquid droplets containing removed microorganisms will then be available for analysis by modern microbiological techniques, such as Real-Time Polymerase Chain Reaction (RT-PCR)...
  12. PHOSPHAZENE POLYMERS FOR DENTAL & BIOMEDICAL APPLICATION
    Lawrence Gettleman; Fiscal Year: 1991
    ..This AREA grant proposal will establish a collaboration between UofL's schools of dentistry and engineering, making use of student workers at both. ..
  13. Diarrhea in Baltimore: Surveillance & Pathogen Detection
    Jon Mark Hirshon; Fiscal Year: 2006
    ..Through this work, we expect to not only better define the causes of diarrheal illnesses in the Baltimore Metropolitan area, but to identify other pathogens that contribute to foodborne illnesses. ..
  14. NURSING STUDY OF SLEEP LOSS AND IMMUNE ACTIVITY
    Carol Landis; Fiscal Year: 1999
    ..Bacteria will be quantified by standard microbiological techniques. Hormones will be measured by HPLC or RIA assays...
  15. Detection of Biofilm Bacteria using GloBac
    Robin Patel; Fiscal Year: 2008
    ..The goal of the proposed studies is to develop a rapid test for detection of bacteria on artificial joints that can be used in the operating room to diagnose infected artificial joints. [unreadable] [unreadable] [unreadable]..
  16. Enhancement of antimicrobics by electrical current
    Robin Patel; Fiscal Year: 2007
    ..This novel but simple resistance reversing strategy may generalize to a wide variety of antimicrobics and microorganisms and a wide variety of biofilm-associated human infections. [unreadable] [unreadable] [unreadable] [unreadable]..
  17. HUMAN RETROVIRUS-5 AND ARTHRITIS
    Robin Patel; Fiscal Year: 2002
    ..Identification of a specific infectious agent associated with these arthritides would potentially allo for the development of preventive strategies such as vaccination and novel therapeutic approaches. ..
  18. RAPID AFFORDABLE TB DRUG SUSCEPTIBILITY TESTING
    Howard Shapiro; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  19. HIGH-PRECISION FLOW CYTOMETRY OF BACTERIA AND VIRUSES
    Howard Shapiro; Fiscal Year: 2004
    ..The relationship between staining intensity and nucleic acid type and content in viruses, will be investigated, with a view toward refining cytometric virus detection and identification. ..
  20. AFFORDABLE RAPID BACTERIAL DETECTION IN STORED PLATELETS
    Howard Shapiro; Fiscal Year: 2005
    ..abstract_text> ..
  21. CYTOMETRIC CD4 COUNTING - A MINIMALIST APPROACH
    Howard Shapiro; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  22. CYTOMETRIC TUBERCULOSIS DETECTION AND SENSITIVITY TESTS
    Howard Shapiro; Fiscal Year: 2005
    ..bovis, M. smegmatis, and M. tuberculosis H37Ra, and evaluate the suitability of fluorescent nitrate reductase substrates for detection and quantification of mycobacterial strains expressing that enzyme. ..
  23. Virological Surveillance of influenza A in Asian lakes
    Scott Rogers; Fiscal Year: 2006
    ..If the lake water and ice are significant reservoirs of influenza, then continued surveillance of arctic lakes may lead to another tool in the battle against this disease. [unreadable] [unreadable]..
  24. Development of Noninvasive Bioluminescence Imaging for Cancer Diagnosis and Thera
    Gary Sayler; Fiscal Year: 2008
    ..This technology will provide the capability for imaging in vivo gene expression in early diagnosis, therapeutic efficacy and disease re-occurrence in whole animal models. [unreadable] [unreadable] [unreadable]..
  25. Pathogen Discovery in Chronic Lung Disease by Mass Tag PCR and Microarray
    W Ian Lipkin; Fiscal Year: 2009
    ....
  26. Behavior Change and Maintenance Intervention for HIV+ MSM Methamphetamine Users
    Thomas L Patterson; Fiscal Year: 2010
    ..g., binge use, injection, polydrug use) and key psychosocial factors (e.g., sexual compulsivity, experiences of abuse) will be explored. ..
  27. Intervention for Female Workers in Mexico
    Thomas Patterson; Fiscal Year: 2008
    ..Subgroup differences in the effectiveness of the intervention based on social, contextual, and interpersonal factors will be explored. ..
  28. CCR5 and CXCR4 Binding Chemokines and Oral HIV Infection
    Grace John Stewart; Fiscal Year: 2005
    ..abstract_text> ..
  29. A Staged Strategy for Virus Identification and Discovery
    W Ian Lipkin; Fiscal Year: 2005
    ..Our initial focus will be two chronic diseases where specific viral taxa are associated and potentially implicated in pathogenesis: bronchioalveolar cell carcinoma and amyotrophic lateral sclerosis. ..
  30. BORNA DISEASE VIRUS AND NEUROPSYCHIATRIC DISEASE
    WALTER LIPKIN; Fiscal Year: 2002
    ..This study will be the first to collect and process human sera and PBMC samples in clinical laboratories with no history of BDV research and dispense aliquots to a central laboratory for randomized, blinded analysis. ..
  31. Phage Binding for Continuous Anthrax Spore Detection
    VALERY PETRENKO; Fiscal Year: 2004
    ..Parallel experiments will also be conducted with antibody-derived probes for comparison evaluation of specificity, selectivity and longevity of the sensors. ..
  32. MOLECULAR ANALYSIS OF A NEUROTROPIC AGENT--BORNA VIRUS
    WALTER LIPKIN; Fiscal Year: 2002
    ....
  33. FOLIC ACID REQUIREMENTS AND ONE CARBON METABOLISM
    Barry Shane; Fiscal Year: 2004
    ....
  34. HIV-1 Specific Cytotoxic T Lymphocytes in Breastmilk
    Grace John Stewart; Fiscal Year: 2003
    ..The resulting findings will be relevant to development of vaccines for prevention of mother-to-child transmission of HIV-1. ..
  35. CONTROL OF PERIODONTAL INFECTIONS
    Sigmund Socransky; Fiscal Year: 2003
    ..Project D will examine the ecological relationships of oral species and their habitat. ..
  36. Characterization of the DNA replication protein Sid2p
    Elizabeth Vallen; Fiscal Year: 2002
    ..Finally, the requirement for various checkpoint and repair pathways will be analyzed to determine the molecular basis of the defect in sid2 mutant cells. ..
  37. MALE GENITAL TRACT RESERVOIRS AND VIRAL SHEDDING
    JOHN KRIEGER; Fiscal Year: 2004
    ..Thus, controlling one genital tract virus may limit viral-viral interactions, reduce the viral burden in semen, and decrease the sexual transmission of other viruses. ..
  38. Functional Skills Training For Late Life Schizophrenia
    Thomas Patterson; Fiscal Year: 2005
    ..Data will allow us to determine short-term effects of our intervention, effectiveness of maintenance sessions, and post-intervention duration of treatment effects. ..
  39. Intra-Oral Biofilm Formation
    Sigmund Socransky; Fiscal Year: 2004
    ..abstract_text> ..
  40. Mass Tag PCR Detection of Respiratory Pathogens
    W Ian Lipkin; Fiscal Year: 2004
    ..abstract_text> ..
  41. Urological Studies of Ideopathic Lower Tract Syndrome
    JOHN KRIEGER; Fiscal Year: 2005
    ..These studies will determine if EPS or SFA can be used to identify prostatic bacteria and may result in clinical methods for non-invasive diagnosis of prostatic infection. ..
  42. UW/USM Chronic Prostatitis CRC
    JOHN KRIEGER; Fiscal Year: 2007
    ..Potential outcomes include: symptom indexes (e.g., the NIH-CPSI), urodynamics and inflammation. ..
  43. Automated Simultaneous Detection of Bioterrorism Agents
    David Ecker; Fiscal Year: 2005
    ..abstract_text> ..
  44. A Staged Strategy for Virus Identification and Discovery
    WALTER LIPKIN; Fiscal Year: 2004
    ..Consistent with aims proposed in AI51292, our initial clinical application will be to analyze enteroviral serotypes implicated in human disease in North America. ..
  45. A DEVELOPMENTAL MODEL FOR AUTISM BASED ON CNS INFECTION
    WALTER LIPKIN; Fiscal Year: 2004
    ..4) Determine how opoptosis contributes to the neuropathology observed after neonatal infection. (5) Analyze regional changes in neurotransmitter systems following neonatal infection. ..
  46. Characterizing Tissue Development Under Biaxial Loading
    Alvin Yeh; Fiscal Year: 2006
    ..This novel experimental platform will form the basis for novel and innovative studies in mechanobiology. ..
  47. Effectiveness of Alcohol Interventions Among TB Patients in Tomsk, Russia
    Sonya Shin; Fiscal Year: 2008
    ..Finally, we have a diverse interdisciplinary team that has been able to formulate an integrated biosocial and culturally-appropriate approach to treating AUDs in this population. [unreadable] [unreadable] [unreadable]..
  48. Human Metapneumovirus in High Risk Populations
    Gregory Gray; Fiscal Year: 2005
    ..These data will facilitate the development of more comprehensive epidemiological studies, and guide future intervention studies in reducing HMPV morbidity. ..
  49. Evaluation of MDR-TB Treatment Strategies in Lima, Peru
    Sonya Shin; Fiscal Year: 2006
    ..abstract_text> ..
  50. Population-based Surveillance for Zoonotic Influenza A
    Gregory Gray; Fiscal Year: 2005
    ..We are particularly well suited to conduct this work as we have extensive experience conducting both epidemiological and laboratory-based studies of respiratory pathogens. ..
  51. VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBC
    Stephen Wagner; Fiscal Year: 2004
    ..abstract_text> ..
  52. ANTHRAX MOLECULAR EVOLUTION, DIVERSITY AND EPIDEMIOLOGY
    Paul Keim; Fiscal Year: 2003
    ..3) Using Monte Carlo simulations, they will ascertain how the distribution of these markers within and between populations of Bacillus anthracis will be affected by geographic, genetic and ecological processes. ..