enzyme stability

Summary

Summary: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.

Top Publications

  1. pmc Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability
    C Vieille
    Biochemistry Department, Michigan State University, East Lansing, Michigan 48824, USA
    Microbiol Mol Biol Rev 65:1-43. 2001
  2. ncbi Developments in the use of Bacillus species for industrial production
    Marcus Schallmey
    Department of Biology, University of Waterloo, ON, Canada
    Can J Microbiol 50:1-17. 2004
  3. pmc How protein stability and new functions trade off
    Nobuhiko Tokuriki
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    PLoS Comput Biol 4:e1000002. 2008
  4. ncbi Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor
    Daumantas Matulis
    Johnson and Johnson Pharmaceutical Research and Development, LLC, Eagleview Corporate Center, 665 Stockton Drive, Suite 104, Exton, Pennsylvania 19341, USA
    Biochemistry 44:5258-66. 2005
  5. ncbi Self-perpetuating states in signal transduction: positive feedback, double-negative feedback and bistability
    James E Ferrell
    Department of Molecular Pharmacology, CCSR, 269 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305 5174, USA
    Curr Opin Cell Biol 14:140-8. 2002
  6. ncbi Intense neutral drifts yield robust and evolvable consensus proteins
    Shimon Bershtein
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel
    J Mol Biol 379:1029-44. 2008
  7. ncbi Evolution of an antibiotic resistance enzyme constrained by stability and activity trade-offs
    Xiaojun Wang
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611 3008, USA
    J Mol Biol 320:85-95. 2002
  8. ncbi Thermotolerant and thermostable laccases
    Kristiina Hildén
    Department of Applied Chemistry and Microbiology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
    Biotechnol Lett 31:1117-28. 2009
  9. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
  10. ncbi Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp. strain AB1 with high stability in organic solvents
    Bassem Jaouadi
    Laboratoire d Enzymes et de Metabolites des Procaryotes LEMP, Centre de biotechnologie de Sfax CBS, B P 1177, Route de Sidi Mansour Km 6, 3018 Sfax, Tunisia
    Bioresour Technol 101:8361-9. 2010

Detail Information

Publications288 found, 100 shown here

  1. pmc Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability
    C Vieille
    Biochemistry Department, Michigan State University, East Lansing, Michigan 48824, USA
    Microbiol Mol Biol Rev 65:1-43. 2001
    ..Finally, current uses and potential applications of thermophilic and hyperthermophilic enzymes as research reagents and as catalysts for industrial processes are described...
  2. ncbi Developments in the use of Bacillus species for industrial production
    Marcus Schallmey
    Department of Biology, University of Waterloo, ON, Canada
    Can J Microbiol 50:1-17. 2004
    ..subtilis 168 and of some related strains, Bacillus species are poised to become the preferred hosts for the production of many new and improved products as we move through the genomic and proteomic era...
  3. pmc How protein stability and new functions trade off
    Nobuhiko Tokuriki
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    PLoS Comput Biol 4:e1000002. 2008
    ..Thus, the evolution of new enzymatic activities, both in nature and in the laboratory, is dependent on the compensatory, stabilizing effect of apparently "silent" mutations in regions of the protein that are irrelevant to its function...
  4. ncbi Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor
    Daumantas Matulis
    Johnson and Johnson Pharmaceutical Research and Development, LLC, Eagleview Corporate Center, 665 Stockton Drive, Suite 104, Exton, Pennsylvania 19341, USA
    Biochemistry 44:5258-66. 2005
    ..The systematic effect of ligands on stability is shown to be a general tool to measure affinity...
  5. ncbi Self-perpetuating states in signal transduction: positive feedback, double-negative feedback and bistability
    James E Ferrell
    Department of Molecular Pharmacology, CCSR, 269 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305 5174, USA
    Curr Opin Cell Biol 14:140-8. 2002
    ..Here we review the basic properties of bistable circuits, the requirements for construction of a satisfactory bistable switch, and the recent progress towards constructing and analysing bistable signaling systems...
  6. ncbi Intense neutral drifts yield robust and evolvable consensus proteins
    Shimon Bershtein
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel
    J Mol Biol 379:1029-44. 2008
    ..The results also suggest that predictable back-to-consensus/ancestor changes can be used in the laboratory to generate highly diverse and evolvable gene libraries...
  7. ncbi Evolution of an antibiotic resistance enzyme constrained by stability and activity trade-offs
    Xiaojun Wang
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611 3008, USA
    J Mol Biol 320:85-95. 2002
    ..This regained stability conferred an advantage in vivo. This pattern of stability loss and restoration may be common in the evolution of new enzyme activity...
  8. ncbi Thermotolerant and thermostable laccases
    Kristiina Hildén
    Department of Applied Chemistry and Microbiology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
    Biotechnol Lett 31:1117-28. 2009
    ..This review gives a current overview on the sources and characteristics of such laccases, with particular emphasis on the fungal enzymes...
  9. ncbi Human liver peroxisomal alanine:glyoxylate aminotransferase: Different stability under chemical stress of the major allele, the minor allele, and its pathogenic G170R variant
    Barbara Cellini
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, Universita degli Studi di Verona, Strada Le Grazie, 8 37134 Verona, Italy
    Biochimie 92:1801-11. 2010
    ..1 M urea. These biochemical results are discussed in the light of the characteristics of the enzymatic phenotype of PH1 patients bearing G170R mutation in AGT-Mi and the positive response of these patients to pyridoxine treatment...
  10. ncbi Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp. strain AB1 with high stability in organic solvents
    Bassem Jaouadi
    Laboratoire d Enzymes et de Metabolites des Procaryotes LEMP, Centre de biotechnologie de Sfax CBS, B P 1177, Route de Sidi Mansour Km 6, 3018 Sfax, Tunisia
    Bioresour Technol 101:8361-9. 2010
    ..These properties make KERAB a potential candidate for future applications in detergent formulations, dehairing during leather processing, and non-aqueous peptide biocatalysis...
  11. ncbi P-27/HP endolysin as antibacterial agent for antibiotic resistant Staphylococcus aureus of human infections
    Ragini Gupta
    Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India
    Curr Microbiol 63:39-45. 2011
    ..aureus 27/HP challenge infection. Thus, P-27/HP endolysin offers suitable substitute of antibiotics to control S. aureus infections...
  12. ncbi Directed evolution of enzyme stability
    Vincent G H Eijsink
    Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, P O Box 5003, N 1432 As, Norway
    Biomol Eng 22:21-30. 2005
    ..Thus, engineering enzyme stability is of both commercial and scientific interest...
  13. pmc Computational thermostabilization of an enzyme
    Aaron Korkegian
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center FHCRC, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 308:857-60. 2005
    ..The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required its activity, thereby coupling molecular and metabolic engineering...
  14. ncbi A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase
    Alexandra Binter
    Institute of Biochemistry, Graz University of Technology, Austria
    FEBS J 276:5263-74. 2009
    ..In all protein variants, this glycine-rich loop exhibits a much higher mobility, compared to the wild-type. This appears to be incompatible with NADPH binding and thus leads to abrogation of flavin reduction...
  15. pmc Characterization of a novel thermostable carboxylesterase from Geobacillus kaustophilus HTA426 shows the existence of a new carboxylesterase family
    Silvia Montoro-García
    Department of Biochemistry and Molecular Biology A, Faculty of Biology, University of Murcia, Campus Espinardo, E30011 Murcia, Spain
    J Bacteriol 191:3076-85. 2009
    ..The topological differences suggested the classification of this enzyme and other Geobacillus-related carboxylesterases in a new alpha/beta hydrolase family different from IV and VI...
  16. pmc Alpha4 is an essential regulator of PP2A phosphatase activity
    Mei Kong
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Cell 36:51-60. 2009
    ..These findings demonstrate that alpha4 plays a required role in regulating the assembly and maintenance of adaptive PP2A phosphatase complexes...
  17. ncbi A novel highly acidic beta-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris
    Huiying Luo
    Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No 12 Zhongguancun South Street, Beijing, 100081, People s Republic of China
    Appl Microbiol Biotechnol 82:453-61. 2009
    ..These favorable properties make MAN5A a potential candidate for use in various industrial applications...
  18. ncbi Insights into thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation
    Jie Liu
    Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
    J Mol Graph Model 27:529-35. 2008
    ..Consequently, we presented strategies to improve thermal stability of the industrialized mesophilic NHase by introducing stable salt-bridge interactions into its thermal sensitive regions...
  19. ncbi A novel beta-mannanase with high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and enzymatic characterization
    Peilong Yang
    Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
    Appl Biochem Biotechnol 159:85-94. 2009
    ..After hydrolysis of LBG, oligomannosides accounted for 76% of the hydrolysis products. All these properties collectively make MAN5A a better candidate than current mannanases for use in the food and feed industry...
  20. ncbi Thermal stability of alpha-amylase in aqueous cosolvent systems
    Jay Kant Yadav
    Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore 570020, India
    J Biosci 34:377-87. 2009
    ..Preferential interaction parameters indicated extensive hydration of the enzyme in the presence of cosolvents...
  21. pmc Disulfide-reduced ALS variants of Cu, Zn superoxide dismutase exhibit increased populations of unfolded species
    Can Kayatekin
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Mol Biol 398:320-31. 2010
    ....
  22. ncbi Mutation-induced loop opening and energetics for binding of tamiflu to influenza N8 neuraminidase
    Parimal Kar
    Department of Theory and Bio Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany
    J Phys Chem B 116:6137-49. 2012
    ..Our study underscores the need to consider dynamics in rationalizing the structure-function relationships of various antiviral inhibitor-NA complexes...
  23. ncbi Directed evolution and structural prediction of cellobiohydrolase II from the thermophilic fungus Chaetomium thermophilum
    Xiu Juan Wang
    Department of Environmental Biology, Shandong Agricultural University, Tai an, Shandong 271018, People s Republic of China
    Appl Microbiol Biotechnol 95:1469-78. 2012
    ..numbers between the 227th and 203rd amino acids were increased, which perhaps contributed to the elevated enzyme stability. Therefore, the two CBHII mutants selected for increased enzymatic activities also demonstrated elevated ..
  24. pmc Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis
    Jared R Auclair
    Department of Chemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 107:21394-9. 2010
    ..In addition, chemical cross-linking conferred activity upon G85R, an otherwise inactive mutant. These results demonstrate that targeting these cysteine residues is an important new strategy for development of ALS therapies...
  25. ncbi Structural features of thermozymes
    W F Li
    Zhejiang University, Animal Science College, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310029, Peoples Republic of China
    Biotechnol Adv 23:271-81. 2005
    ..Understanding of the stabilizing features will greatly facilitate reengineering of some of the mesozymes to more stable thermozymes...
  26. ncbi Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus
    Chun Zhang
    Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, Chongqing, China
    Biosci Biotechnol Biochem 74:1298-301. 2010
    ..Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol)...
  27. pmc Accessory mutations maintain stability in drug-resistant HIV-1 protease
    Max W Chang
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Mol Biol 410:756-60. 2011
    ..These findings help us to explain the prevalence of mutations located far from the active site and underscore the importance of protein stability during the evolution of drug resistance in HIV...
  28. pmc Biochemical analyses are instrumental in identifying the impact of mutations on holo and/or apo-forms and on the region(s) of alanine:glyoxylate aminotransferase variants associated with primary hyperoxaluria type I
    Elisa Oppici
    Department of Life Sciences and Reproduction, Section of Biological Chemistry, University of Verona, Strada Le Grazie 8 37134 Verona, Italy
    Mol Genet Metab 105:132-40. 2012
    ..e. active site microenvironment, large and/or small domain) of the protein are affected by each mutation, and (iii) suggest the possible therapeutic approach for patients bearing the examined mutations...
  29. ncbi Cloning and enzymatic characterization of a xylanase gene from a soil-derived metagenomic library with an efficient approach
    Yong Hu
    The College of Life Sciences, Hubei University, Wuhan 430062, China
    Appl Microbiol Biotechnol 80:823-30. 2008
    ....
  30. ncbi Listeria bacteriophage peptidoglycan hydrolases feature high thermoresistance and reveal increased activity after divalent metal cation substitution
    Mathias Schmelcher
    Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092, Zurich, Switzerland
    Appl Microbiol Biotechnol 93:633-43. 2012
    ..The available data suggest that denaturation at elevated temperatures is reversible and may be followed by rapid refolding into a functional state...
  31. pmc Mutation that blocks ATP binding creates a pseudokinase stabilizing the scaffolding function of kinase suppressor of Ras, CRAF and BRAF
    Jiancheng Hu
    Department of Pathology and Immunology, Howard Hughes Medical Institute, Washington University School of Medicine, 660 South Euclid, Box 8118, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 108:6067-72. 2011
    ..Not only does this work suggest that KSR1 may function as a kinase, we anticipate that the mutation that we generated may be broadly applicable to stabilize the closed conformation of other kinases many of which may also form dimers...
  32. ncbi Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus
    Renda Hawwa
    Department of Medicinal Chemistry and Pharmacognosy and the Center for Pharmaceutical Biotechnology, University of Illinois at Chicago, Chicago, IL 60607, USA
    Arch Biochem Biophys 488:109-20. 2009
    ..Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs...
  33. pmc Structural and chemical profiling of the human cytosolic sulfotransferases
    Abdellah Allali-Hassani
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
    PLoS Biol 5:e97. 2007
    ..The data help explain substrate promiscuity in this family and, at the same time, reveal new similarities between hSULT family members that were previously unrecognized by sequence or structure comparison alone...
  34. ncbi Immobilization of lactase from Kluyveromyces lactis greatly reduces the inhibition promoted by glucose. full hydrolysis of lactose in milk
    Cesar Mateo
    Departamento de Biocatalisis, Instituto de Catalisis, CSIC, Campus Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    Biotechnol Prog 20:1259-62. 2004
    ....
  35. ncbi In vivo molecular evolution reveals biophysical origins of organismal fitness
    Rafael Couñago
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
    Mol Cell 22:441-9. 2006
    ..The changes in organismal fitness were linked directly to incremental increases in enzyme stability and activity maxima and corresponded to the narrow temperature ranges where each mutant enjoyed success ..
  36. ncbi Identifying and engineering ion pairs in adenylate kinases. Insights from molecular dynamics simulations of thermophilic and mesophilic homologues
    Euiyoung Bae
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 280:30943-8. 2005
    ..These results provide specific knowledge about stability in adenylate kinases and more generally suggest that molecular dynamics simulations can provide valuable information for identifying and engineering ion pairs...
  37. pmc Enzymatic analysis of an amylolytic enzyme from the hyperthermophilic archaeon Pyrococcus furiosus reveals its novel catalytic properties as both an alpha-amylase and a cyclodextrin-hydrolyzing enzyme
    Sung Jae Yang
    Department of Food Science and Technology, Seoul National University, Shillim Dong, Kwanak gu, Seoul 151 742, Korea
    Appl Environ Microbiol 70:5988-95. 2004
    ..These results confirm that PFTA possesses novel catalytic properties characteristic of both alpha-amylase and cyclodextrin-hydrolyzing enzyme...
  38. ncbi Properties and applications of starch-converting enzymes of the alpha-amylase family
    Marc J E C van der Maarel
    Microbial Physiology Research Group, Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands
    J Biotechnol 94:137-55. 2002
    ....
  39. ncbi Chemical chaperones improve transport and enhance stability of mutant alpha-glucosidases in glycogen storage disease type II
    Toshika Okumiya
    Department of Clinical Genetics, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Mol Genet Metab 90:49-57. 2007
    ..Chemical chaperone therapy could create new perspectives for therapeutic intervention in GSDII...
  40. ncbi Selection of mutations for increased protein stability
    Bertus van den Burg
    IMEnz Bioengineering BV, Kerklaan 30, PO Box 14, Haren, The Netherlands
    Curr Opin Biotechnol 13:333-7. 2002
    ..Selection is complicated by lack of knowledge of the process that leads to thermal inactivation and by the fact that proteins employ a large variety of structural tricks to achieve stability...
  41. ncbi Characterization and structural modeling of a new type of thermostable esterase from Thermotoga maritima
    Mark Levisson
    Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 GT Wageningen, The Netherlands
    FEBS J 274:2832-42. 2007
    ..A comparison of the active site pentapeptide motifs revealed that EstD should be grouped into a new family of esterases (Family 10). EstD is the first characterized member of this family...
  42. ncbi Cloning, sequencing and expression in Escherichia coli of a thermophilic lipase from Bacillus thermoleovorans ID-1
    A R Cho
    Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, 134 Shinchon dong, Sudaemun Ku, Seoul, South Korea
    FEMS Microbiol Lett 186:235-8. 2000
    ..For expression in Escherichia coli, the lipase gene was subcloned in pET-22b(+) vector with a strong T7 promoter. Lipase activity was approximately 1.4-fold greater than under the native promoter...
  43. ncbi Stabilization against thermal inactivation promoted by sugars on enzyme structure and function: why is trehalose more effective than other sugars?
    M Sola-Penna
    Departamento de Fármacos, Faculdade de Farmacia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21944 910, Brasil
    Arch Biochem Biophys 360:10-4. 1998
    ..Finally, these data drive us to conclude that the higher size exclusion effect is responsible for the difference in efficiency of protection against thermal inactivation of enzymes...
  44. ncbi Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design
    Oliver Spadiut
    Department of Food Sciences and Technology, BOKU University of Natural Resources and Applied Life Sciences, Vienna, Austria
    FEBS J 276:776-92. 2009
    ..Distinct differences, however, are observed for the P2Ox Leu537 variants at the interfaces between the subunits, which results in tighter association...
  45. pmc Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum
    Renate Reiss
    Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials, Lerchenfeldstr, 5, 9014 St, Gallen, Switzerland
    BMC Biotechnol 11:9. 2011
    ..Owing to their broad substrate range laccases are considered to be versatile biocatalysts which are capable of oxidizing natural and non-natural industrial compounds, with water as sole by-product...
  46. ncbi Electrostatic interactions across the dimer-dimer interface contribute to the pH-dependent stability of a tetrameric malate dehydrogenase
    Alexandra Bjørk
    Department of Biology, University of Oslo, Box 1031 Blindern, N 0316 Oslo, Norway
    FEBS Lett 553:423-6. 2003
    ..8 degrees C, and depended strongly on pH. Gel-filtration experiments indicated that the 26.8 degrees C loss in stability observed for the D56K mutant at low pH was accompanied by a shift towards a lower oligomerization state...
  47. ncbi The refined crystal structure of Bacillus cereus oligo-1,6-glucosidase at 2.0 A resolution: structural characterization of proline-substitution sites for protein thermostabilization
    K Watanabe
    Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Japan
    J Mol Biol 269:142-53. 1997
    ..This well supports the previous finding that the replacement at the appropriate positions in beta-turns or alpha-helices is the most effective for protein thermostabilization by proline substitution...
  48. ncbi Thermozymes and their applications: a review of recent literature and patents
    M E Bruins
    Department of Food Technology and Nutritional Sciences, Wageningen University, The Netherlands
    Appl Biochem Biotechnol 90:155-86. 2001
    ..With wider availability and lower cost, thermophilic enzymes will see more application in industry...
  49. pmc How active-site protonation state influences the reactivity and ligation of the heme in chlorite dismutase
    Bennett R Streit
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Am Chem Soc 132:5711-24. 2010
    ..0(+/-0.6) x 10(5) s(-1), k(cat)/K(M) = 3.2(+/-0.4) x 10(7) M(-1) s(-1), pH 5.2, 4 degrees C) and to a somewhat lesser extent when it acts as a H-bond donor to the axial hydroxide ligand under alkaline conditions...
  50. pmc Identification of small molecule inhibitors of the mitotic kinase haspin by high-throughput screening using a homogeneous time-resolved fluorescence resonance energy transfer assay
    Debasis Patnaik
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Biomol Screen 13:1025-34. 2008
    ..These leads form the basis for the development of specific inhibitors of haspin that will have clear utility in basic research and possible use as starting points for development of antimitotic anticancer therapeutics...
  51. ncbi Characterization of proline-serine-rich carboxyl terminus in human sulfotransferase 2B1b: immunogenicity, subcellular localization, kinetic properties, and phosphorylation
    Dongning He
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 1670 University Blvd, Volker Hall, Room G133M, Birmingham, AL 35294, USA
    Drug Metab Dispos 34:1749-55. 2006
    ..This study suggests that the PSC extension of SULT2B1b is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of this SULT isoform...
  52. ncbi Consequences of missense mutations for dimerization and turnover of alanine:glyoxylate aminotransferase: study of a spectrum of mutations
    M B Coulter-Mackie
    Department of Pediatrics, University of British Columbia, Children s and Women s Health Centre, Vancouver, BC, Canada
    Mol Genet Metab 89:349-59. 2006
    ..The minor allele AGT product and many of the mutants were subject to a limited non-proteasomal proteolytic cleavage when ATP was depleted...
  53. pmc The diversity of the catalytic properties of class A beta-lactamases
    A Matagne
    Universite de Liege, Institute de Chimie, Belgium
    Biochem J 265:131-46. 1990
    ..values than class C enzymes, thus belying the usual distinction between 'penicillinases' and 'cephalosporinases'. The problem of the repartition of class A beta-lactamases into sub-classes is discussed...
  54. ncbi Biochemical properties and substrate specificities of alkaline and histidine acid phytases
    B C Oh
    Microbial Genomics Laboratory, Korea Research Institute of Bioscience and Biotechnology, P O Box 115, 305 600 Yusong, Taejon, Korea
    Appl Microbiol Biotechnol 63:362-72. 2004
    ..This review describes recent findings that present novel viewpoints concerning the molecular basis of phytase classification...
  55. ncbi Deduced amino-acid sequence of a calcium-free alpha-amylase from a strain of Bacillus: implications from molecular modeling of high oxidation stability and chelator resistance of the enzyme
    H Hagihara
    Tochigi Research Laboratories of Kao Corporation, Ichikai, Haga, Japan
    Eur J Biochem 268:3974-82. 2001
    ..By building a molecular model, we showed the calcium-independent, oxidatively stable active-site topology and structural integrity of AmyK38...
  56. ncbi A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme
    I Lascu
    Unité de Biochimie Cellulaire URA 1129, Institut Pasteur, Paris, France
    J Biol Chem 267:12775-81. 1992
    ..This suggests that the mutation affects the folding/assembly pathway without affecting the function of the mature protein once folded and assembled into the mature hexameric structure...
  57. ncbi Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil
    H Noureddini
    Department of Chemical Engineering, 207h Othmer Hall, University of Nebraska Lincoln, Lincoln, NE 68588 0643, USA
    Bioresour Technol 96:769-77. 2005
    ..The immobilized lipase also proved to be stable and lost little activity when was subjected to repeated uses...
  58. pmc Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3
    Christian Ottmann
    Chemical Genomics Centre, Otto Hahn Strasse 15, 44227 Dortmund, Germany
    Proc Natl Acad Sci U S A 106:17223-8. 2009
    ..Although Ca(2+)-binding sites are conserved and critical for stability in other subtilases, SBT3 was found to be Ca(2+)-free and its thermo stability is Ca(2+)-independent...
  59. pmc Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta
    F Boudsocq
    Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, MD 20892 2725, USA
    Nucleic Acids Res 29:4607-16. 2001
    ..Thus, although phylogenetically related to DinB polymerases, our studies suggest that the archaeal Dpo4 enzyme exhibits lesion-bypass properties that are, in fact, more akin to those of eukaryotic poleta...
  60. ncbi Copper incorporation into recombinant CotA laccase from Bacillus subtilis: characterization of fully copper loaded enzymes
    Paulo Durão
    Universidade Nova de Lisboa, Av da Republica, 2781 901, Oeiras, Portugal
    J Biol Inorg Chem 13:183-93. 2008
    ..EPR and resonance Raman data indicate that, presumably, folding in the presence of copper is indispensable for the correct structure of the trinuclear copper-containing site...
  61. ncbi A thermoalkaliphilic lipase of Geobacillus sp. T1
    Thean Chor Leow
    Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia
    Extremophiles 11:527-35. 2007
    ..Serine and aspartate residues were involved in catalysis, as its activity was strongly inhibited by 5 mM PMSF and 1 mM Pepstatin. The T(m) for T1 lipase was around 72.2 degrees C, as revealed by denatured protein analysis of CD spectra...
  62. ncbi Thermostable enzymes as biocatalysts in the biofuel industry
    Carl J Yeoman
    Institute for Genomic Biology, University of Illinois, Urbana, Illinois, USA
    Adv Appl Microbiol 70:1-55. 2010
    ..Furthermore, we discuss the protein structure, mechanisms of thermostability, and specific strategies that can be used to improve the thermal stability of lignocellulosic biocatalysts...
  63. ncbi Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina
    Gui Min Zhang
    College of Life Science, Hubei University, Wuhan 430062, People s Republic of China
    Appl Microbiol Biotechnol 74:339-46. 2007
    ..It is interesting to note that Cu2+ ion, which strongly inhibits most other xylanases studied, reduces enzyme activity by only 40%. Furthermore, enzyme activity is unaffected by EDTA even at a concentration of 5 mM...
  64. ncbi Immobilised enzymes: science or art?
    Linqiu Cao
    DMV International, P O Box 13, 5460 BA, Veghel, The Netherlands
    Curr Opin Chem Biol 9:217-26. 2005
    ..e. non-covalent adsorption, covalent binding, entrapment and encapsulation) or with other relevant technologies. The objective is to solve specific problems that cannot be solved by one of these basic immobilisation techniques...
  65. ncbi SNPs, protein structure, and disease
    Z Wang
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Hum Mutat 17:263-70. 2001
    ..In sharp contrast, over 70% of the population set are found to be neutral. The remaining 30% are potentially involved in polygenic disease...
  66. pmc Amino acid residues in the N-terminal region of the PA subunit of influenza A virus RNA polymerase play a critical role in protein stability, endonuclease activity, cap binding, and virion RNA promoter binding
    Koyu Hara
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    J Virol 80:7789-98. 2006
    ..These results suggest that the N-terminal region of PA is involved in multiple functions of the polymerase, including protein stability, endonuclease activity, cap binding, and promoter binding...
  67. ncbi Catalytic versatility, stability, and evolution of the (betaalpha)8-barrel enzyme fold
    Reinhard Sterner
    Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
    Chem Rev 105:4038-55. 2005
  68. pmc Isolation and characterization of metalloproteases with a novel domain structure by construction and screening of metagenomic libraries
    Tanja Waschkowitz
    Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg August Universität, Grisebachstr 8, 37077 Gottingen, Germany
    Appl Environ Microbiol 75:2506-16. 2009
    ..The optimum pH and temperature of both proteases were 8.0 and 65 degrees C, respectively, when casein was used as substrate...
  69. ncbi Stability of biocatalysts
    Karen M Polizzi
    School of Chemical and Biomolecular Engineering, Parker H Petit Institute of Bioengineering and Bioscience, USA
    Curr Opin Chem Biol 11:220-5. 2007
    ..Clearly, more effort is needed to study how solution conditions impact protein kinetic stability...
  70. pmc A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro
    Yan Wang
    Department of Research and Development, MJ Bioworks Inc, 7000 Shoreline Court, South San Francisco, CA 94080, USA
    Nucleic Acids Res 32:1197-207. 2004
    ..dsDNA binding protein to a polymerase can increase processivity without compromising catalytic activity and enzyme stability. Second, polymerase processivity is limiting for the efficiency of PCR, such that the fusion enzymes exhibit ..
  71. pmc Mannose 6-phosphate receptor-mediated transport of sulfamidase across the blood-brain barrier in the newborn mouse
    Akihiko Urayama
    Department of Neurology, Laboratory of Protein Misfolding Disorders, University of Texas Medical Branch, Galveston, Texas, USA
    Mol Ther 16:1261-6. 2008
    ..That is, the transport of sulfamidase into the brain parenchyma in early postnatal life is mediated by the M6P receptor, which is shared with P-GUS and is likely accessible to other M6P-containing lysosomal enzymes...
  72. ncbi Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii
    Patrick Murray
    Molecular Glycobiotechnology Group, Department of Biochemistry, National University of Ireland, Galway, Ireland
    Protein Expr Purif 38:248-57. 2004
    ..254 mM). Cel3a was also active against natural cellooligosacharides with glucose being the product of hydrolysis. It displayed transferase activity producing mainly cellobiose from glucose and cellotetrose from cellobiose...
  73. ncbi Functional synergism between the most common polymorphism in human alanine:glyoxylate aminotransferase and four of the most common disease-causing mutations
    M J Lumb
    Medical Research Council Laboratory for Molecular Cell Biology and the Department of Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 275:36415-22. 2000
    ..These results are discussed in the light of the high allelic frequency ( approximately 20%) of the P11L polymorphism and its importance in determining the phenotypic manifestations of mutations in PH1...
  74. pmc Hot spots in cold adaptation: localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes
    P A Fields
    Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950 3094, USA
    Proc Natl Acad Sci U S A 95:11476-81. 1998
    ..However, at a common temperature of measurement, the higher configurational entropy of a cold-adapted enzyme may foster conformations that bind ligands poorly, leading to high Km values relative to warm-adapted orthologs...
  75. ncbi Missense polymorphism in the human carboxypeptidase E gene alters enzymatic activity
    H Chen
    Millennium Pharmaceuticals, Inc, Cambridge, Massachusetts 02139, USA
    Hum Mutat 18:120-31. 2001
    ..We predict that this mutant could cause hyperproinsulinism and diabetes in the homozygous state...
  76. pmc Decreased stability and increased formation of soluble aggregates by immature superoxide dismutase do not account for disease severity in ALS
    Kenrick A Vassall
    Department of Chemistry, Guelph Waterloo Centre for Graduate Studies in Chemistry and Biochemistry, Waterloo, ON, Canada N2L 3G1
    Proc Natl Acad Sci U S A 108:2210-5. 2011
    ..Rather, additional and/or multiple forms of SOD1 and additional biophysical and biological factors are needed to account for the toxicity of mutant SOD1 in ALS...
  77. ncbi The (1)H, (13)C, (15)N resonance assignment, solution structure, and residue level stability of eosinophil cationic protein/RNase 3 determined by NMR spectroscopy
    Douglas V Laurents
    Instituto de Quimica Fisica Rocasolano, CSIC, Madrid, Spain
    Biopolymers 91:1018-28. 2009
    ..Analysis of their exchange rates reveals a strikingly high global stability of 11.8 kcal/mol. This value and other stability measurements are used to better quantify ECP's unfolding thermodynamics...
  78. pmc Conformational change in the C-terminal domain is responsible for the initiation of creatine kinase thermal aggregation
    Hua Wei He
    Department of Biological Sciences and Biotechnology, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    Biophys J 89:2650-8. 2005
    ..Our results also suggested that the changes in domain-domain interactions, but not the dissociation of the dimer, might play a crucial role in the thermal denaturation and aggregation of this dimeric two-domain protein...
  79. ncbi Cloning and functional expression of an acidophilic β-mannanase gene (Anman5A) from Aspergillus niger LW-1 in Pichia pastoris
    Jian Fang Li
    School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People s Republic of China
    J Agric Food Chem 60:765-73. 2012
    ..Its activity was not significantly affected by an array of metal ions and ethylenediaminetetraacetic acid (EDTA). The K(m) and V(max) of the reAnMan5A, toward locust bean gum, were 1.10 mg/mL and 266.7 units/mg, respectively...
  80. ncbi An alkaline-active and alkali-stable pectate lyase from Streptomyces sp. S27 with potential in textile industry
    Peng Yuan
    Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No 12 Zhongguancun South Street, Beijing 100081, People s Republic of China
    J Ind Microbiol Biotechnol 39:909-15. 2012
    ..49.7%). When combined with cellulase and α-amylase, r-PL-STR had comparable performance in bioscouring of jute fabric (22.39 vs. 22.99%). Thus, r-PL-STR might represent a good candidate for use in alkaline industries such as textile...
  81. ncbi Conformational and thermal stability of mature dimeric human myeloperoxidase and a recombinant monomeric form from CHO cells
    Srijib Banerjee
    Division of Biochemistry, Department of Chemistry, Vienna Institute of BioTechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria
    Biochim Biophys Acta 1814:375-87. 2011
    ..Spectroscopic and thermodynamic data of unfolding are discussed with respect to the known three-dimensional structure of leukocyte MPO as well as to known physiological roles...
  82. ncbi Differential roles of Arg97, Asp293, and Arg108 in enzyme stability and substrate specificity of CYP2C9
    Leslie J Dickmann
    Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA
    Mol Pharmacol 65:842-50. 2004
    ..These data identify critical roles for Arg97 and Asp293 in the structural stability of the enzyme and demonstrate a selective role for Arg108 in the binding and metabolism of negatively charged substrates of CYP2C9...
  83. pmc Engineering of Clostridium phytofermentans Endoglucanase Cel5A for improved thermostability
    Wenjin Liu
    Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Appl Environ Microbiol 76:4914-7. 2010
    ..The best mutant had 92%, 36%, and 46% longer half-lives at 60 degrees C on carboxymethyl cellulose, regenerated amorphous cellulose, and Avicel, respectively...
  84. ncbi Accurate stabilities of laccase mutants predicted with a modified FoldX protocol
    Niels J Christensen
    Technical University of Denmark, DTU Chemistry, Kemitorvet 206, Kongens Lyngby, DK 2800 Denmark
    J Chem Inf Model 52:3028-42. 2012
    ..The developed model provides insight into the physical forces governing the high stability of fungal laccases, most notably the hydrophobic and van der Waals interactions in the folded state, which provide most of the predictive power...
  85. pmc Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast
    Eva García-Ruiz
    Department of Biocatalysis, Institute of Catalysis, Madrid, Spain
    Microb Cell Fact 9:17. 2010
    ..The current work describes how to achieve this goal by engineering ligninolytic oxidoreductases (a high-redox potential laccase -HRPL- and a versatile peroxidase, -VP-) functionally expressed in Saccharomyces cerevisiae...
  86. ncbi Enhancement of the thermostability and the catalytic efficiency of Bacillus pumilus CBS protease by site-directed mutagenesis
    Bassem Jaouadi
    Laboratoire d Enzymes et de Métabolites des Procaryotes, Centre de biotechnologie de Sfax CBS, Route de Sidi Mansour Km 6, Sfax, Tunisia
    Biochimie 92:360-9. 2010
    ....
  87. ncbi Conformational stability and integrity of alpha-amylase from mung beans: evidence of kinetic intermediate in GdmCl-induced unfolding
    Pallavi Tripathi
    School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
    Biophys Chem 137:95-9. 2008
    ..These results suggest that the first (reversible) step of unfolding is slower than the second (irreversible) step at low GdmCl concentrations, whereas the rates of these two steps are opposite at high GdmCl concentrations...
  88. ncbi A chloride tolerant laccase from the plant pathogen ascomycete Botrytis aclada expressed at high levels in Pichia pastoris
    Roman Kittl
    Food Biotechnology Laboratory, Department of Food Sciences and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biotechnol 157:304-14. 2012
    ..The high expression yield permits fast and easy production for further basic and applied research...
  89. pmc Comparative hydrogen-deuterium exchange for a mesophilic vs thermophilic dihydrofolate reductase at 25 °C: identification of a single active site region with enhanced flexibility in the mesophilic protein
    Olayinka A Oyeyemi
    Department of Chemistry, California Institute for Quantitative Biosciences QB3, University of California, Berkeley, Berkeley, California 94720, USA
    Biochemistry 50:8251-60. 2011
    ..The region with increased flexibility in Ec-DHFR corresponds to one of two regions previously proposed to control the enthalpic barrier for hydride transfer in Bs-DHFR [Oyeyemi et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 10074]...
  90. ncbi GH11 xylanases: Structure/function/properties relationships and applications
    Gabriel Paës
    INRA, UMR614 FARE, 2 Esplanade Roland Garros, F 51686 Reims, France
    Biotechnol Adv 30:564-92. 2012
    ..GH11 xylanases have for a long time been used as biotechnological tools in various industrial applications and represent in addition promising candidates for future other uses...
  91. pmc Comparative analyses of two thermophilic enzymes exhibiting both beta-1,4 mannosidic and beta-1,4 glucosidic cleavage activities from Caldanaerobius polysaccharolyticus
    Yejun Han
    Energy Biosciences Institute, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 192:4111-21. 2010
    ..Furthermore, on the basis of the biochemical and genomic data, a molecular mechanism for utilization of mannan-containing nutrients by C. polysaccharolyticus is proposed...
  92. ncbi Truncation of the cellulose binding domain improved thermal stability of endo-beta-1,4-glucanase from Bacillus subtilis JA18
    Yujuan Wang
    Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, PR China
    Bioresour Technol 100:345-9. 2009
    ..UV spectrometry analysis showed Egl330 and Egl499 differed in refolding efficiency after heat treatment...
  93. ncbi Laccases for removal of recalcitrant and emerging pollutants
    Josée Anne Majeau
    INRS ETE, Universite du Quebec, 490 de la Couronne, Quebec, Canada G1K 9A9
    Bioresour Technol 101:2331-50. 2010
    ..Hence, a global overview of parameters affecting the biocatalysis of pollutants by laccases, particularly with regard to the economical production of these enzymes using synthetic media and waste materials, is timely...
  94. ncbi The conserved N-terminal helix of acylpeptide hydrolase from archaeon Aeropyrum pernix K1 is important for its hyperthermophilic activity
    Zuoming Zhang
    Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, PR China
    Biochim Biophys Acta 1784:1176-83. 2008
    ..Since the N-terminal helix is conserved in this family of enzymes, our results suggest that the N-terminal helix is likely to play an important role for stabilizing all other enzymes in this family...
  95. ncbi New isolate of Streptomyces sp. with novel thermoalkalotolerant cellulases
    Faiez Alani
    Biochemical Engineering Group, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada N2L 3G1
    Biotechnol Lett 30:123-6. 2008
    ..After incubation at 40 degrees C for 1 h with commercial detergent (Tide) at pH 11, 95% activity was retained. The enzyme mixture produced glucose from crystalline cellulose...
  96. pmc Structural changes to monomeric CuZn superoxide dismutase caused by the familial amyotrophic lateral sclerosis-associated mutation A4V
    Tom Schmidlin
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Biophys J 97:1709-18. 2009
    ....
  97. ncbi Exploring the dihydrodipicolinate synthase tetramer: how resilient is the dimer-dimer interface?
    Michael D W Griffin
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia
    Arch Biochem Biophys 494:58-63. 2010
    ..Despite only weak destabilisation of the tetramer by some mutations, catalytic activity was reduced to approximately 10-15% of the wild-type in all cases, suggesting that the dimer-dimer interface is finely tuned to optimise function...
  98. pmc Identification and characterization of ambroxol as an enzyme enhancement agent for Gaucher disease
    Gustavo H B Maegawa
    Division of Clinical and Metabolic Genetics, Department of Paediatrics, The Hospital for Sick Children, 555 University Ave, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 284:23502-16. 2009
    ..Thus, ABX has the biochemical characteristics of a safe and effective enzyme enhancement therapy agent for the treatment of patients with the most common GD genotypes...
  99. ncbi Isolation and properties of fungal beta-glucosidases
    O G Korotkova
    Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia
    Biochemistry (Mosc) 74:569-77. 2009
    ..verruculosum and T. reesei exhibiting relatively high activity to polysaccharide substrates and lower activity to low molecular weight substrates (activity to cellobiose was lower than to pNPG)...
  100. ncbi Characterization of a novel acylaminoacyl peptidase with hexameric structure and endopeptidase activity
    Zoltan Szeltner
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary
    Biochim Biophys Acta 1794:1204-10. 2009
    ....
  101. ncbi Characterization of D-tagatose-3-epimerase from Rhodobacter sphaeroides that converts D-fructose into D-psicose
    Longtao Zhang
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, People s Republic of China
    Biotechnol Lett 31:857-62. 2009
    ..Its activity was maximal at pH 9 and 40 degrees C while being enhanced by Mn(2+). At pH 9 and 40 degrees C, 118 g D-psicose l(-1) was produced from 700 g D-fructose l(-1) after 3 h...

Research Grants24

  1. Elucidating a novel molecular biomarker for castration-resistant prostate cancer
    Nima Sharifi; Fiscal Year: 2013
    ..mutation occurs in 3[unreadable]HSD1 in the development of a subset of CRPC cases, increases mutant enzyme stability, augments DHT synthesis and confers resistance to abiraterone acetate...
  2. DNA Sequencing and Nanotechnology
    Nyoté J Oliver; Fiscal Year: 2012
    ..for bioreactors, including the facile separation of enzyme from products and in some cases, increased enzyme stability and activity...
  3. A Micromachined, Multielectrode Microprobe for Monitoring Glutamate and Dopamine
    HAROLD GEORGE MONBOUQUETTE; Fiscal Year: 2010
    ..A novel technology to encapsulate glutamate oxidase in porous polymer nanoparticles will be used to improve enzyme stability and to enable its selective electrodeposition on microelectrodes...
  4. Inhibition of Resistant Variants of HIV Protease
    Irene T Weber; Fiscal Year: 2013
    ..for resistance including mutations that: 1) decrease protease interactions with inhibitors;2) decrease the enzyme stability;or 3) increase the flap mobility...
  5. PEG-MODIFIED ENZYMES FOR IN-VIVO DECON OF OP TOXINS
    KEITH LEJEUNE; Fiscal Year: 2003
    ..conducive to use as an in-vivo medical treatment for a variety of reasons including acute antigenicity, poor enzyme stability, and brief in-vivo residence times...
  6. IMPROVING BIOAVAILABILITY OF RGD PEPTIDOMIMETICS
    Teruna Siahaan; Fiscal Year: 2000
    ..is due to their physicochemical properties , including size, charge, solubility, hydrogen-bonding potential, enzyme stability and conformation...
  7. GLUCOSE SENSITIVE MEMBRANES IN THE DELIVERY OF INSULIN
    THOMAS HORBETT; Fiscal Year: 1992
    ..Both of these device configurations are specifically proposed as methods to overcome potential limitations (due to enzyme stability and biocompatibility) of previous monolithic, long term implant designs.
  8. PEG-MODIFIED ENZYMES FOR IN-VIVO DECON OF OP TOXINS
    KEITH LEJEUNE; Fiscal Year: 2001
    ..such as sarin and soman as well as many commercially available pesticides including acute antigenicity, poor enzyme stability, and brief in-vivo residence times...
  9. FORMATION AND SURVIVAL OF RED BLOOD CELLS
    Ernest Beutler; Fiscal Year: 2001
    ..Wild type and mutant enzymes will then be compared with respect to Km for substrate and enzyme stability. Where possible, reference to the crystal structure of the enzyme (or, related structure) will be made...
  10. RELATIONSHIP BETWEEN ENZYME STABILITY & ENZYME FUNCTION
    CARLOS PACE; Fiscal Year: 2001
    ..This can have future potential for redesign of enzymes to generate the desired catalytic properties and stability. ..
  11. HTS Assay to Profile Glucuronidation of Small Molecules
    Vladimir Trubetskoy; Fiscal Year: 2005
    ..HTS UGT assays have failed, due to the challenges of working with dilute membrane enzyme preparations [low enzyme stability, significant light scattering, low signal-to-noise ratios, and low assay variability (Z'-factor)]...
  12. Phosphomimics and Serotonin Acetyltransferase Regulation
    LAWRENCE SZEWCZUK; Fiscal Year: 2007
    ..Phosphorylation of AANAT promotes binding to 14-3-3 gamma which increases enzyme stability (e.g. resistance to proteases) and activity thus promoting melatonin synthesis...
  13. Protein Dynamics and Flexibility in Enzyme Catalysis
    Katherine Henzler Wildman; Fiscal Year: 2006
    ..These experiments will provide quantitative evidence to test the hypothesis that high enzyme activity results from a delicate balance of enzyme stability and flexibility.
  14. Conference--Neuronal Ceroid Lipofuscinosis
    Glyn Dawson; Fiscal Year: 2003
    ..abstract_text> ..
  15. Influence of Heme on Hepatic Cytochrome P450 Synthesis and Degradation
    Maria Correia; Fiscal Year: 2007
    ..These studies are expected to elucidate how heme can affect the birth and death of these P450 proteins and thus modulate the effects and elimination of ingested drugs and environmental agents in man. ..
  16. Structure of signal peptide peptidase
    RAQUEL LIEBERMAN; Fiscal Year: 2008
    ..This first structure of an intramembrane protease will provide critical insight into the biochemistry of intramembrane proteolysis and enable structure- based AD drug development and screening. ..
  17. Structure/Function Studies on Flavoproteins
    Irina Sevrioukova; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  18. Single Molecule Studies of DNA Helicases
    PIERO BIANCO; Fiscal Year: 2007
    ..abstract_text> ..
  19. Activated Sugars for Drug Discovery
    Ryan Woodyer; Fiscal Year: 2006
    ..These sugars can be used to make derivatives of natural products with new therapeutic properties, for example, antibiotics that are effective against antibiotic-resistant bacteria. [unreadable] [unreadable] [unreadable]..
  20. STRUCTURAL & ENERGETIC PRINCIPLES OF MEMBRANE PROTEINS
    KAREN FLEMING; Fiscal Year: 2002
    ....
  21. Tenth International Congress on Ceroid Lipofuscinoses
    Glyn Dawson; Fiscal Year: 2005
    ....
  22. MECHANISMS OF PYRIDOXAL-5'-PHOSPHATE DEPENDENT ENZYMES
    Robert Phillips; Fiscal Year: 2004
    ..Thus the results of this research are likely to make a significant contribution to the goal of improving health care for Americans in the future. ..
  23. Hexameric PBGS as a Bioterrorism Defense
    Eileen Jaffe; Fiscal Year: 2006
    ..Success of the proposed studies will establish a much needed new target for species selective antibiotic development that can be applied to bio-defense against NIAID priority pathogens. ..
  24. Bioinformatics for Protein Microarrays
    Richard Zangar; Fiscal Year: 2009
    ....