ankyrin repeat

Summary

Summary: Protein motif that contains a 33-amino acid long sequence that often occurs in tandem arrays. This repeating sequence of 33-amino acids was discovered in ANKYRIN where it is involved in interaction with the anion exchanger (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE). Ankyrin repeats cooperatively fold into structures that mediate molecular recognition via protein-protein interactions.

Top Publications

  1. ncbi A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein
    Snezana Kojic
    Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, Serbia
    Arch Biochem Biophys 502:60-7. 2010
  2. pmc Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors
    Xiaoxiao Pan
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 320:1651-4. 2008
  3. pmc TolC-dependent secretion of an ankyrin repeat-containing protein of Rickettsia typhi
    Simran J Kaur
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    J Bacteriol 194:4920-32. 2012
  4. pmc Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins
    Matthew E Cockman
    Central Proteomics Facility, University of Oxford, Oxford, United Kingdom
    Mol Cell Proteomics 8:535-46. 2009
  5. ncbi High-affinity binders selected from designed ankyrin repeat protein libraries
    H Kaspar Binz
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Nat Biotechnol 22:575-82. 2004
  6. ncbi Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
    H Kaspar Binz
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Mol Biol 332:489-503. 2003
  7. pmc The diversity and evolution of Wolbachia ankyrin repeat domain genes
    Stefanos Siozios
    Department of Environmental and Natural Resources Management, University of Western Greece, Agrinio, Greece
    PLoS ONE 8:e55390. 2013
  8. pmc Differential regulation of TRPV1, TRPV3, and TRPV4 sensitivity through a conserved binding site on the ankyrin repeat domain
    Christopher B Phelps
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 285:731-40. 2010
  9. pmc The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion
    Daniel E Voth
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 191:4232-42. 2009
  10. ncbi Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size
    Christian Zahnd
    Universitat Zurich, Biochemisches Institut, Winterthurerstrasse, 8057 Zurich, Switzerland
    Cancer Res 70:1595-605. 2010

Detail Information

Publications246 found, 100 shown here

  1. ncbi A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein
    Snezana Kojic
    Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, Serbia
    Arch Biochem Biophys 502:60-7. 2010
    The muscle ankyrin repeat protein (MARP) family member Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation ..
  2. pmc Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors
    Xiaoxiao Pan
    Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 320:1651-4. 2008
    ..IV secretion system to deliver into eukaryotic cells a large number of different bacterial proteins containing ankyrin repeat homology domains called Anks. The L...
  3. pmc TolC-dependent secretion of an ankyrin repeat-containing protein of Rickettsia typhi
    Simran J Kaur
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    J Bacteriol 194:4920-32. 2012
    ..In this study, we characterized a gene (RT0218) encoding a C-terminal ankyrin repeat domain-containing protein, named Rickettsia ankyrin repeat protein 1 (RARP-1), and identified it as a secreted ..
  4. pmc Proteomics-based identification of novel factor inhibiting hypoxia-inducible factor (FIH) substrates indicates widespread asparaginyl hydroxylation of ankyrin repeat domain-containing proteins
    Matthew E Cockman
    Central Proteomics Facility, University of Oxford, Oxford, United Kingdom
    Mol Cell Proteomics 8:535-46. 2009
    ..In support of this certain ankyrin repeat domain (ARD)-containing proteins, including members of the IkappaB and Notch families, have been identified as ..
  5. ncbi High-affinity binders selected from designed ankyrin repeat protein libraries
    H Kaspar Binz
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Nat Biotechnol 22:575-82. 2004
    We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding...
  6. ncbi Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
    H Kaspar Binz
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Mol Biol 332:489-503. 2003
    We describe an efficient way to generate combinatorial libraries of stable, soluble and well-expressed ankyrin repeat (AR) proteins...
  7. pmc The diversity and evolution of Wolbachia ankyrin repeat domain genes
    Stefanos Siozios
    Department of Environmental and Natural Resources Management, University of Western Greece, Agrinio, Greece
    PLoS ONE 8:e55390. 2013
    b>Ankyrin repeat domain-encoding genes are common in the eukaryotic and viral domains of life, but they are rare in bacteria, the exception being a few obligate or facultative intracellular Proteobacteria species...
  8. pmc Differential regulation of TRPV1, TRPV3, and TRPV4 sensitivity through a conserved binding site on the ankyrin repeat domain
    Christopher B Phelps
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 285:731-40. 2010
    ..TRPV1-V4, have large cytoplasmic regions flanking the transmembrane domain, including an N-terminal ankyrin repeat domain...
  9. pmc The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion
    Daniel E Voth
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 191:4232-42. 2009
    ..Genes encoding proteins with eukaryotic-type ankyrin repeat domains (Anks) were identified upon genome sequencing of the C...
  10. ncbi Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size
    Christian Zahnd
    Universitat Zurich, Biochemisches Institut, Winterthurerstrasse, 8057 Zurich, Switzerland
    Cancer Res 70:1595-605. 2010
    ..We show here that with very small high-affinity proteins such as designed ankyrin repeat proteins (DARPins), these limits can be overcome...
  11. ncbi A designed ankyrin repeat protein evolved to picomolar affinity to Her2
    Christian Zahnd
    Biochemisches Institut der Universitat Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    J Mol Biol 369:1015-28. 2007
    Designed ankyrin repeat proteins (DARPins) are a novel class of binding molecules, which can be selected to recognize specifically a wide variety of target proteins...
  12. pmc ankA: an Ehrlichia phagocytophila group gene encoding a cytoplasmic protein antigen with ankyrin repeats
    P Caturegli
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 68:5277-83. 2000
    ..phagocytophila group bacteria and also detected antigen associated with chromatin in infected but not uninfected HL-60 cell cultures. These results suggest that this Ehrlichia protein may influence host cell gene expression...
  13. pmc Consensus-derived structural determinants of the ankyrin repeat motif
    Leila K Mosavi
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Proc Natl Acad Sci U S A 99:16029-34. 2002
    The ankyrin repeat is one of the most common, modular, protein-protein interaction motifs in nature...
  14. pmc Hypoxia-dependent sequestration of an oxygen sensor by a widespread structural motif can shape the hypoxic response--a predictive kinetic model
    Bernhard Schmierer
    Oxford Centre for Integrative Systems Biology OCISB, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    BMC Syst Biol 4:139. 2010
    ..Competition by ARD proteins for FIH is hypothesised to affect FIH activity towards HIFα; however the extent of this competition and its effect on the HIF-dependent hypoxic response are unknown...
  15. pmc Structural basis of competition between PINCH1 and PINCH2 for binding to the ankyrin repeat domain of integrin-linked kinase
    Brian P Chiswell
    Department of Pharmacology, 333 Cedar Street, Yale University School of Medicine, New Haven, CT 06520, USA
    J Struct Biol 170:157-63. 2010
    ..Here we report that the LIM1 domains of PINCH1 and PINCH2 directly compete for the same binding site on the ankyrin repeat domain (ARD) of ILK. We determined the 1...
  16. ncbi Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism
    Andreas Schweizer
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Structure 15:625-36. 2007
    ..We selected a designed ankyrin repeat protein (DARPin) from a combinatorial library that inhibits caspase-2 in vitro with a subnanomolar inhibition ..
  17. pmc A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end
    Ludovic Pecqueur
    Laboratoire d Enzymologie et Biochimie Structurales, Centre de Recherche de Gif, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 109:12011-6. 2012
    ..Here, we identified a designed ankyrin repeat protein (DARPin) that interferes with microtubule assembly in a unique manner...
  18. ncbi Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins
    Svava K Wetzel
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Mol Biol 376:241-57. 2008
    Full-consensus designed ankyrin repeat proteins were designed with one to six identical repeats flanked by capping repeats. These proteins express well in Escherichia coli as soluble monomers...
  19. pmc Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains
    Ming Yang
    Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, UK
    FEBS J 278:1086-97. 2011
    ..C-terminal transcriptional activation domain (HIF-CAD), FIH also catalyses asparaginyl hydroxylation of many ankyrin repeat domain-containing proteins, revealing a broad sequence selectivity...
  20. pmc Structural analyses of the ankyrin repeat domain of TRPV6 and related TRPV ion channels
    Christopher B Phelps
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 01238, USA
    Biochemistry 47:2476-84. 2008
    ..All members of the vanilloid family of TRP channels (TRPV) possess an N-terminal ankyrin repeat domain (ARD)...
  21. pmc A primer on ankyrin repeat function in TRP channels and beyond
    Rachelle Gaudet
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 01238, USA
    Mol Biosyst 4:372-9. 2008
    ..Recent progress in the field has provided new insights into the structure and function of the ankyrin repeat motifs present in the N-terminal cytosolic domain of many TRP channels...
  22. ncbi Investigating the dependence of the hypoxia-inducible factor hydroxylases (factor inhibiting HIF and prolyl hydroxylase domain 2) on ascorbate and other reducing agents
    Emily Flashman
    Chemistry Research Laboratory and the Oxford Centre for Integrative Systems Biology, University of Oxford, Mansfield Road, Oxford OX13TA, U K
    Biochem J 427:135-42. 2010
    ..and of FIH-catalysed hydroxylation of asparaginyl hydroxylation sites in HIF-1alpha and in a consensus ankyrin repeat domain peptide...
  23. ncbi Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle
    Ilona A Barash
    Dept of Orthopaedics, University of California, Department of Veterans Affairs Medical Center, San Diego, CA 92161, USA
    Am J Physiol Cell Physiol 293:C218-27. 2007
    ..However, the cellular basis for this response remains unclear. Previous studies identified the muscle ankyrin repeat protein (MARP) family, consisting of cardiac ankyrin repeat protein (CARP), ankyrin repeat domain 2/ankyrin ..
  24. ncbi Chaperone-assisted crystallography with DARPins
    Gaby Sennhauser
    Department of Biochemistry, University of Zurich, CH 8057 Zurich, Switzerland
    Structure 16:1443-53. 2008
    ..A valuable addition to the present repertoire of binding proteins is the recently developed designed ankyrin repeat protein (DARPin) technology...
  25. ncbi The role of cell signaling in poxvirus tropism: the case of the M-T5 host range protein of myxoma virus
    Steven J Werden
    Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Biochim Biophys Acta 1784:228-37. 2008
    ....
  26. ncbi ASB proteins interact with Cullin5 and Rbx2 to form E3 ubiquitin ligase complexes
    Junya Kohroki
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, Noda shi, Chiba 278 8510, Japan
    FEBS Lett 579:6796-802. 2005
    The ankyrin repeat and SOCS box (ASB) family is composed of 18 proteins from ASB1 to ASB18 and belongs to the suppressor of cytokine signaling (SOCS) box protein superfamily...
  27. ncbi Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor
    Mathew L Coleman
    Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, United Kingdom
    J Biol Chem 282:24027-38. 2007
    ..factor inhibiting HIF (FIH), also catalyzes hydroxylation of highly conserved asparaginyl residues within ankyrin repeat (AR) domains (ARDs) of endogenous Notch receptors...
  28. ncbi Biophysical characterisation of the small ankyrin repeat protein myotrophin
    Alan R Lowe
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge, CB2 2XZ, UK
    J Mol Biol 365:1245-55. 2007
    ..Myotrophin is the first example of a naturally occurring ankyrin repeat protein that conforms to an apparent two-state mechanism at equilibrium and under kinetic conditions, making ..
  29. ncbi The enigmatic role of the ankyrin repeat domain 1 gene in heart development and disease
    Alexander T Mikhailov
    Developmental Biology Group, Institute of Health Sciences, University of La Coruna, La Coruna, Spain
    Int J Dev Biol 52:811-21. 2008
    It has been proposed that the ankyrin repeat domain 1 (ANKRD1) factor (also known as CARP) plays a critical role in transcriptional regulation, myofibrillar assembly and stretch sensing during heart development and cardiac insults...
  30. ncbi A role for the Ankyrin repeat containing protein Ankrd17 in Nod1- and Nod2-mediated inflammatory responses
    Maureen Menning
    Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Germany
    FEBS Lett 587:2137-42. 2013
    ..In conclusion, this reveals a novel function for Ankrd17 in anti-bacterial innate immune pathways...
  31. pmc CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue
    Yubin Shi
    Department of Pathology C3321 MCN, Vanderbilt University School of Medicine, Nashville, TN 37232 2562, USA
    Am J Pathol 166:303-12. 2005
    Cardiac ankyrin repeat protein (CARP) was identified by subtractive hybridization as one of a group of genes that are rapidly modulated by acute wounding of mouse skin...
  32. ncbi CARP, a cardiac ankyrin repeat protein, is downstream in the Nkx2-5 homeobox gene pathway
    Y Zou
    American Heart Association Bugher Foundation Center for Molecular Biology, Department of Medicine, University of California, San Diego, La Jolla 92093, USA
    Development 124:793-804. 1997
    ..In a search for interacting co-factors, a nuclear ankyrin-like repeat protein CARP (cardiac ankyrin repeat protein) was isolated from a rat neonatal heart cDNA library by yeast two-hybrid screening, using YB-1 as the ..
  33. ncbi Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway
    R J Diederich
    Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06536 0812
    Development 120:473-81. 1994
    ..addition, we present a new Notch allele, Nsu42c, that is associated with a missense mutation within the fifth ankyrin repeat. In addition to representing a new class of viable Notch allele, this mutation behaves similarly to mutations ..
  34. ncbi BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
    Chan Man Ha
    Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Plant Cell Physiol 45:1361-70. 2004
    ..This is the first report on a plant protein that plays a key role in morphogenesis with the distinctive combinatorial architecture of the BTB/POZ and ankyrin repeat domains.
  35. ncbi The ankyrin repeat gene family in rice: genome-wide identification, classification and expression profiling
    Jianyan Huang
    National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research Wuhan, Huazhong Agricultural University, 430070 Wuhan, China
    Plant Mol Biol 71:207-26. 2009
    b>Ankyrin repeat (ANK) containing proteins comprise a large protein family. Although many members of this family have been implicated in plant growth, development and signal transduction, only a few ANK genes have been reported in rice...
  36. ncbi Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module
    Michaela A Kramer
    Department of Biochemistry, University of Zurich, Winterthurerstr 190, 8057 Zurich, Switzerland
    J Mol Biol 404:381-91. 2010
    Designed ankyrin repeat proteins (DARPins) that specifically bind to almost any target can be obtained by ribosome display or phage display from combinatorial libraries...
  37. ncbi Directionality of temperature activation in mouse TRPA1 ion channel can be inverted by single-point mutations in ankyrin repeat six
    Sairam Jabba
    Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 82:1017-31. 2014
    ..All mutations are located in ankyrin repeat six, which identifies this domain as a sensitive modulator of thermal activation...
  38. ncbi Designed ankyrin repeat proteins: a novel tool for testing epidermal growth factor receptor 2 expression in breast cancer
    Jean Philippe Theurillat
    Department Pathology, Institute of Surgical Pathology, University Hospital of Zurich, Zurich, Switzerland
    Mod Pathol 23:1289-97. 2010
    Designed ankyrin repeat proteins are a novel class of specific binding molecules, which display increased thermodynamic stability, smaller size and at least equal target affinity compared to immunoglobulins, making them potentially ..
  39. pmc The ankyrin repeat domain of the TRPA protein painless is important for thermal nociception but not mechanical nociception
    Richard Y Hwang
    Department of Neurobiology, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 7:e30090. 2012
    ..Thus, we propose that in the case of Painless, ankryin repeats are important for thermal nociception but not for mechanical nociception...
  40. pmc Folding kinetics of the cooperatively folded subdomain of the IκBα ankyrin repeat domain
    Ingrid Devries
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0378, USA
    J Mol Biol 408:163-76. 2011
    The ankyrin repeat (AR) domain of IκBα consists of a cooperative folding unit of roughly four ARs (AR1-AR4) and of two weakly folded repeats (AR5 and AR6)...
  41. ncbi Designed ankyrin repeat protein binders for the crystallization of AcrB: plasticity of the dominant interface
    Nicole Monroe
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Struct Biol 174:269-81. 2011
    ..We observe that small variations in the interface can lead to differing behavior of the DARPins with regards to affinity, stoichiometry of the complexes and specificity for their target...
  42. ncbi F-box-like domains are present in most poxvirus ankyrin repeat proteins
    Andrew A Mercer
    Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin, New Zealand
    Virus Genes 31:127-33. 2005
    ..The large number of different poxviral ANK-F-box proteins suggests a wide range of cellular proteins might be subjected to ubiquitin-mediated degradation, thereby modulating diverse cellular responses to viral infection...
  43. pmc The ankyrin-repeat transmembrane protein BDA1 functions downstream of the receptor-like protein SNC2 to regulate plant immunity
    Yuanai Yang
    State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, People s Republic of China 102206
    Plant Physiol 159:1857-65. 2012
    ..These data suggest that BDA1 is a critical signaling component that functions downstream of SNC2 to regulate plant immunity...
  44. pmc Visualization of the nanospring dynamics of the IkappaBalpha ankyrin repeat domain in real time
    Jorge A Lamboy
    Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92092 0378, USA
    Proc Natl Acad Sci U S A 108:10178-83. 2011
    ..IκBα has a weakly folded ankyrin repeat 5-6 (AR5-6) region that is critical in establishing its short intracellular half-life...
  45. pmc Structural and biochemical consequences of disease-causing mutations in the ankyrin repeat domain of the human TRPV4 channel
    Hitoshi Inada
    Department of Molecular and Cellular Biology, Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA
    Biochemistry 51:6195-206. 2012
    ..Recent studies reported that mutations in TRPV4, including some in its ankyrin repeat domain (ARD), are associated with human inherited diseases, including neuropathies and skeletal dysplasias, ..
  46. ncbi DARPins as bispecific receptor antagonists analyzed for immunoglobulin E receptor blockage
    Alexander Eggel
    Institute of Immunology, University of Bern, Inselspital, Sahlihaus 2, Bern, Switzerland
    J Mol Biol 393:598-607. 2009
    ..Here, we propose an alternative and simple way to generate bispecific binding molecules using designed ankyrin repeat proteins (DARPins)...
  47. pmc Crystal structure of the human TRPV2 channel ankyrin repeat domain
    Clare J McCleverty
    Genomics Institute of the Novartis Research Foundation, CA 92121, USA
    Protein Sci 15:2201-6. 2006
    ..An ankyrin repeat domain (ARD), which is a common protein-protein recognition domain, is conserved in the N-terminal ..
  48. pmc Folding landscapes of ankyrin repeat proteins: experiments meet theory
    Doug Barrick
    T C Jenkins Department of Biophysics, The Johns Hopkins University, 3400N, Charles St Baltimore, MD 21218, USA
    Curr Opin Struct Biol 18:27-34. 2008
    Nearly 6% of eukaryotic protein sequences contain ankyrin repeat (AR) domains, which consist of several repeats and often function in binding. AR proteins show highly cooperative folding despite a lack of long-range contacts...
  49. pmc Full reconstruction of a vectorial protein folding pathway by atomic force microscopy and molecular dynamics simulations
    Whasil Lee
    Center for Biologically Inspired Materials and Material Systems and Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, USA
    J Biol Chem 285:38167-72. 2010
    ..to the full reconstruction of the vectorial folding pathway of a large polypeptide, the 253-residue consensus ankyrin repeat protein, NI6C...
  50. pmc Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling
    Ykelien L Boersma
    Department of Biochemistry, University of Zurich, 8057 Zurich, Switzerland
    J Biol Chem 286:41273-85. 2011
    ..Designed ankyrin repeat proteins (DARPins) may provide the targeting component for such novel reagents...
  51. ncbi Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments
    Gianluca Interlandi
    Department of Biochemistry, University of Zurich, CH 8057 Zurich, Switzerland
    J Mol Biol 375:837-54. 2008
    ..simulations with explicit solvent at room temperature and at 400 K were carried out to characterize designed ankyrin repeat (AR) proteins with full-consensus repeats...
  52. ncbi Molecular characterization of rice OsBIANK1, encoding a plasma membrane-anchored ankyrin repeat protein, and its inducible expression in defense responses
    Xinchun Zhang
    State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310029, Hangzhou, Zhejiang, People s Republic of China
    Mol Biol Rep 37:653-60. 2010
    A rice gene, OsBIANK1, encoding a protein containing a typical ankyrin repeat domain, was cloned and identified...
  53. pmc Anchoring skeletal muscle development and disease: the role of ankyrin repeat domain containing proteins in muscle physiology
    Jin Ming Tee
    Hubrecht Institute for Developmental Biology and Stem Cell Research University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Crit Rev Biochem Mol Biol 45:318-30. 2010
    The ankyrin repeat is a protein module with high affinity for other ankyrin repeats based on strong Van der Waals forces...
  54. ncbi DARPins against a functional IgE epitope
    Michael J Baumann
    Institute of Immunology, University of Bern, Inselspital, Sahlihaus 2, 3010 Bern, Switzerland
    Immunol Lett 133:78-84. 2010
    ..For this purpose, DARPins™ (designed ankyrin repeat proteins) against the constant heavy chain region of IgE have been isolated...
  55. pmc Characterization of an ankyrin repeat-containing Shank2 isoform (Shank2E) in liver epithelial cells
    Ryan R McWilliams
    Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80439, USA
    Biochem J 380:181-91. 2004
    ..binding protein 1) or Spank-3] has been described as a truncated family member without an N-terminal ankyrin repeat domain...
  56. pmc Ankyrin Repeat-rich Membrane Spanning/Kidins220 protein regulates dendritic branching and spine stability in vivo
    Synphen H Wu
    Molecular Neurobiology Program, Skirball Institute of Biomolecular Medicine, Department of Physiology and Neuroscience, New York University School of Medicine, New York, New York 10016, USA
    Dev Neurobiol 69:547-57. 2009
    ..Here we report that a substrate of BDNF signaling, the Ankyrin Repeat-rich Membrane Spanning (ARMS) protein or Kidins220, plays a critical role in the branching of cortical and ..
  57. pmc Hierarchical phosphorylation within the ankyrin repeat domain defines a phosphoregulatory loop that regulates Notch transcriptional activity
    Prathibha Ranganathan
    Molecular Oncology Program, Division of Surgical Oncology, Dewitt Daughtry Family of Surgery and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami Florida 33136, USA
    J Biol Chem 286:28844-57. 2011
    ..This study identifies a new component involved in regulation of Notch(ICD) transcriptional activity, reinforcing the notion that a precise and tight regulation is required for this essential signaling pathway...
  58. pmc MNF, an ankyrin repeat protein of myxoma virus, is part of a native cellular SCF complex during viral infection
    Sophie Blanié
    INRA, UMR 1225 Interactions Hôtes Agents Pathogènes F 31076 Toulouse, France
    Virol J 7:56. 2010
    ..For the first time, endogenous components of SCF (Cullin-1 and Skp1) were co-precipitated with an ANK myxoma virus protein, expressed in an infectious context, and without over-expression of any protein...
  59. ncbi Asparaginyl beta-hydroxylation of proteins containing ankyrin repeat domains influences their stability and function
    Adam P Hardy
    Department of Chemistry and the Oxford Centre for Integrative Systems Biology, The Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK
    J Mol Biol 392:994-1006. 2009
    Recent reports have provided evidence that the beta-hydroxylation of conserved asparaginyl residues in ankyrin repeat domain (ARD) proteins is a common posttranslational modification in animal cells...
  60. ncbi The muscle ankyrin repeat proteins are hypoxia-sensitive: in vivo mRNA expression in the hypoxia-tolerant blind subterranean mole rat, Spalax ehrenbergi
    Mark Band
    WM Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, USA
    J Mol Evol 70:1-12. 2010
    The muscle ankyrin repeat proteins (MARPs), also known as muscle stretch proteins, are members of a conserved family of genes known to be induced under stress conditions...
  61. pmc The ankyrin repeat as molecular architecture for protein recognition
    Leila K Mosavi
    MC3305, Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA
    Protein Sci 13:1435-48. 2004
    The ankyrin repeat is one of the most frequently observed amino acid motifs in protein databases...
  62. pmc ANKYRIN REPEAT-CONTAINING PROTEIN 2A is an essential molecular chaperone for peroxisomal membrane-bound ASCORBATE PEROXIDASE3 in Arabidopsis
    Guoxin Shen
    Department of Biological Sciences, Texas Tech University, Lubbock, Texas 79409, USA
    Plant Cell 22:811-31. 2010
    Arabidopsis thaliana ANKYRIN REPEAT-CONTAINING PROTEIN 2A (AKR2A) interacts with peroxisomal membrane-bound ASCORBATE PEROXIDASE3 (APX3). This interaction involves the C-terminal sequence of APX3 (i.e...
  63. ncbi Ankyrin repeat: a unique motif mediating protein-protein interactions
    Junan Li
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 45:15168-78. 2006
    b>Ankyrin repeat, one of the most widely existing protein motifs in nature, consists of 30-34 amino acid residues and exclusively functions to mediate protein-protein interactions, some of which are directly involved in the development of ..
  64. pmc Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance
    Yong Sheng Wang
    Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA
    Plant Cell 18:3635-46. 2006
    ..XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for ..
  65. ncbi Cardiac ankyrin repeat protein is a novel marker of cardiac hypertrophy: role of M-CAT element within the promoter
    Y Aihara
    Second Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan
    Hypertension 36:48-53. 2000
    ..Moreover, given that activation of these pathways is associated with cardiac hypertrophy, we propose that CARP represents a novel genetic marker of cardiac hypertrophy...
  66. ncbi Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4
    B T Kile
    The Walter and Eliza Hall Institute for Medical Research and The Cooperative Research Centre for Cellular Growth Factors, Post Office, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
    Gene 258:31-41. 2000
    ..We report here the cloning, characterization, mapping and expression analysis of four members of the ankyrin repeat and SOCS box-containing (Asb) protein family.
  67. ncbi Characterization of human skeletal muscle Ankrd2
    A Pallavicini
    CRIBI Biotechnology Centre, Universita degli Studi di Padova, Via Ugo Bassi 58B, I 35121 Padua, Italy
    Biochem Biophys Res Commun 285:378-86. 2001
    ..This is particularly evident in dystrophic muscles, where the expression of Ankrd2 in slow fibers seems to be severely reduced...
  68. ncbi Notch-regulated ankyrin-repeat protein inhibits Notch1 signaling: multiple Notch1 signaling pathways involved in T cell development
    Theodore J Yun
    IDEC Pharmaceuticals, San Diego, CA 92121, USA
    J Immunol 170:5834-41. 2003
    ..The different effects of Deltex-1 and Nrarp overexpression suggest that alternate Notch signaling pathways mediate T vs B lineage commitment and thymocyte maturation...
  69. ncbi Hypothesis: a helix of ankyrin repeats of the NOMPC-TRP ion channel is the gating spring of mechanoreceptors
    Jonathon Howard
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Curr Biol 14:R224-6. 2004
  70. pmc A novel ankyrin-repeat membrane protein, IGN1, is required for persistence of nitrogen-fixing symbiosis in root nodules of Lotus japonicus
    Hirotaka Kumagai
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8602, Japan
    Plant Physiol 143:1293-305. 2007
    ....
  71. ncbi Regulation of Wolbachia ankyrin domain encoding genes in Drosophila gonads
    George Papafotiou
    Institute of Cellular and Developmental Biology, Biomedical Sciences Research Center Alexander Fleming, Vari 16672, Greece
    Res Microbiol 162:764-72. 2011
    ..Intriguingly, the Wolbachia genome codes for an unusually high number of ankyrin repeat (ANK) proteins. ANKs mediate protein-protein interactions in many different contexts...
  72. pmc Mechanical unfolding of an ankyrin repeat protein
    David Serquera
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge, United Kingdom
    Biophys J 98:1294-301. 2010
    ..Here we use atomic force microscopy and molecular-dynamics simulations to investigate the natural 7-ankyrin repeat protein gankyrin. We find that gankyrin unfolds under force via multiple distinct pathways...
  73. pmc Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy
    Lewyn Li
    Biophys J 90:L30-2. 2006
    Using single-molecule atomic force microscopy, we find that a protein consisting of six identical ankyrin repeat units flanked by N- and C-terminal modules (N6C) unfolds in a stepwise, unit-by-unit fashion under a mechanical force...
  74. ncbi Signalling cross talk of the HIF system: involvement of the FIH protein
    M L Coleman
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Curr Pharm Des 15:3904-7. 2009
    ..are targeted for hydroxylation at conserved Asn residues within a protein interaction domain known as the Ankyrin Repeat Domain (ARD). Many ARD-containing proteins bind to FIH more tightly than does HIF...
  75. pmc Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase
    Yanwu Yang
    Department of Molecular Cardiology, Lerner Research Institute, The Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 284:5836-44. 2009
    ..PINCH complex (28 kDa, K(D) approximately 68 nm) involving the N-terminal ankyrin repeat domain (ARD) of ILK and the first LIM domain (LIM1) of PINCH...
  76. ncbi Stabilizing ionic interactions in a full-consensus ankyrin repeat protein
    Tobias Merz
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Mol Biol 376:232-40. 2008
    Full-consensus designed ankyrin repeat proteins (DARPins), in which randomized positions of the previously described DARPin library have been fixed, are characterized...
  77. pmc CD4-specific designed ankyrin repeat proteins are novel potent HIV entry inhibitors with unique characteristics
    Andreas Schweizer
    Division of Infectious Diseases, University Hospital Zurich, Zurich, Switzerland
    PLoS Pathog 4:e1000109. 2008
    Here, we describe the generation of a novel type of HIV entry inhibitor using the recently developed Designed Ankyrin Repeat Protein (DARPin) technology...
  78. ncbi FIH-dependent asparaginyl hydroxylation of ankyrin repeat domain-containing proteins
    Matthew E Cockman
    Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, United Kingdom
    Ann N Y Acad Sci 1177:9-18. 2009
    ..Proteins containing a common interaction motif known as the ankyrin repeat domain (ARD) have been shown to be efficiently hydroxylated by FIH...
  79. ncbi MYPT1, the targeting subunit of smooth-muscle myosin phosphatase, is a substrate for the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH)
    James D Webb
    Nuffield Department of Clinical Medicine, University of Oxford, Headington, UK
    Biochem J 420:327-33. 2009
    ..More recently, several ARD [AR (ankyrin repeat) domain]-containing proteins were identified as FIH substrates using FIH interaction assays...
  80. pmc The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules
    Robert E Collins
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Nat Struct Mol Biol 15:245-50. 2008
    ..Here we demonstrate that the ankyrin repeat domains of G9a and GLP bind with strong preference to N-terminal H3 peptides containing mono- or dimethyl K9...
  81. ncbi Characterization and transcriptional analysis of protein tyrosine phosphatase genes and an ankyrin repeat gene of the parasitoid Glyptapanteles indiensis polydnavirus in the parasitized host
    D E Gundersen-Rindal
    US Department of Agriculture, Agricultural Research Service, Insect Biocontrol Laboratory, Bldg 011A, Room 214, BARC West, Beltsville, MD 20705, USA
    J Gen Virol 87:311-22. 2006
    ..6 kb segment shown to encode nine protein tyrosine phosphatase (PTP) genes and a single ankyrin repeat gene (ank), is analysed...
  82. pmc Expression of Anaplasma marginale ankyrin repeat-containing proteins during infection of the mammalian host and tick vector
    Solomon S Ramabu
    Department of Veterinary Microbiology and Pathology, and The Paul G Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 79:2847-55. 2011
    ..Selection for divergence in Ank function among Anaplasma and Ehrlichia spp. is supported by both locus and allelic analyses of genes encoding orthologous proteins and their ankyrin motif compositions...
  83. pmc The Notch-regulated ankyrin repeat protein is required for proper anterior-posterior somite patterning in mice
    Luke T Krebs
    Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074, USA
    Genesis 50:366-74. 2012
    The Notch-regulated ankyrin repeat protein (Nrarp) is a component of a negative feedback system that attenuates Notch pathway-mediated signaling...
  84. ncbi Treatment of diabetic macular edema with a designed ankyrin repeat protein that binds vascular endothelial growth factor: a phase I/II study
    Peter A Campochiaro
    The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 9277, USA
    Am J Ophthalmol 155:697-704, 704.e1-2. 2013
    To evaluate the safety and bioactivity of MP0112, a designed ankyrin repeat protein (DARPin) that specifically binds vascular endothelial growth factor (VEGF) in patients with diabetic macular edema (DME)...
  85. ncbi Designed ankyrin repeat proteins (DARPins) from research to therapy
    Rastislav Tamaskovic
    Department of Biochemistry, University of Zurich, Winterthurerstrasse, Zurich, Switzerland
    Methods Enzymol 503:101-34. 2012
    Designed ankyrin repeat proteins (DARPins) have been developed into a robust and versatile scaffold for binding proteins. High-affinity binders are routinely selected by ribosome display and phage display...
  86. pmc Regulation of nucleocytoplasmic shuttling of Bruton's tyrosine kinase (Btk) through a novel SH3-dependent interaction with ankyrin repeat domain 54 (ANKRD54)
    Manuela O Gustafsson
    Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden
    Mol Cell Biol 32:2440-53. 2012
    ..characterize the interaction with ankryin repeat domain 54 protein (ANKRD54), also known as Lyn-interacting ankyrin repeat protein (Liar)...
  87. pmc Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-α (HIF-α) versus ankyrin repeat substrates
    Sarah E Wilkins
    School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 287:8769-81. 2012
    ..HIF (FIH) catalyzes the β-hydroxylation of asparagine residues in HIF-α transcription factors as well as ankyrin repeat domain (ARD) proteins such as Notch and Gankyrin...
  88. ncbi Single-molecule experiments in vitro and in silico
    Marcos Sotomayor
    Department of Physics, University of Illinois at Urbana Champaign, and Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, IL 61801, USA
    Science 316:1144-8. 2007
    ....
  89. ncbi XNAP, a conserved ankyrin repeat-containing protein with a role in the Notch pathway during Xenopus primary neurogenesis
    Katia Lahaye
    Universite Libre de Bruxelles, IBMM, Rue des Professeurs Jeener et Brachet 12, B 6041 Gosselies, Belgium
    Mech Dev 110:113-24. 2002
    ..Together, these data indicate that XNAP is a novel target of the Notch pathway that may, in a feedback loop, modulate its activity...
  90. ncbi Designed ankyrin repeat proteins as anti-idiotypic-binding molecules
    Monique Vogel
    Institute of Immunology, Inselspital, 3010 Bern, Switzerland
    Ann N Y Acad Sci 1109:9-18. 2007
    ..Designed ankyrin repeat proteins (DARPins) are a new class of binding proteins and may serve as an alternative to antibodies...
  91. ncbi An ankyrin repeat protein is involved in anthocyanin biosynthesis in Arabidopsis
    Jihye Yoo
    Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin 446 701, Korea
    Physiol Plant 142:314-25. 2011
    ..A novel putative phytochrome-interacting ankyrin repeat protein 2 (PIA2) containing three repeated ankyrin domains was identified in Arabidopsis...
  92. ncbi Assignment of ankyrin repeat, family A (RFXANK-like) 2 (ANKRA2) to human chromosome 5q12-->q13 by radiation hybrid mapping and somatic cell hybrid PCR
    K Rader
    Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla CA, USA
    Cytogenet Cell Genet 89:164-5. 2000
  93. pmc Is AKR2A an essential molecular chaperone for a class of membrane-bound proteins in plants?
    Hong Zhang
    Department of Biological Sciences, Texas Tech University, Lubbock, Texas, USA
    Plant Signal Behav 5:1520-2. 2010
    ..at least 500 genes encoding proteins that contain sequences similar to ABS, which raises interesting questions: are these proteins AKR2A's ligand proteins and does AKR2A play a critical role in the biogenesis of these proteins in plants?..
  94. ncbi In silico structural and functional analysis of fragments of the ankyrin repeat protein p18(INK4c)
    P Sklenovsý
    Department of Physical Chemistry and Center for Biomolecules and Complex Molecular Systems, Palacký University at Olomouc tr 17 listopadub 12, 771 46 Olomouc Czech Republic
    J Biomol Struct Dyn 27:521-40. 2010
    b>Ankyrin repeat proteins (ARPs) are ubiquitous proteins that play critical regulatory roles in organisms and consist of repeating motifs (ankyrin repeats) stacked in non-globular, almost linear, "quasi one-dimensional" configurations...
  95. ncbi PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62
    Changgen Xie
    College of Life Sciences, Peking University, Beijing, China
    J Genet Genomics 37:359-69. 2010
    ..Our results suggest that PKS5 functions at the upstream of NPR1 and might mediate expression of WRKY38 and WRKY62 possibly by interacting with and phosphorylating NPR1...
  96. ncbi Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR
    Svava K Wetzel
    Institute of Biochemistry, University of Zurich, CH 8057 Zurich, Switzerland
    J Mol Biol 402:241-58. 2010
    We investigated the stability determinants and the unfolding characteristics of full-consensus designed ankyrin repeat proteins (DARPins) by NMR...
  97. ncbi A novel ankyrin repeat-rich gene in potato, Star, involved in response to late blight
    Tian Wu
    Key Laboratory of Horticulture Plant Biology, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan, China
    Biochem Genet 47:439-50. 2009
    The Solanum tuberosum ankyrin repeat gene (Star) is a novel gene from potato leaves challenged by Phytophthora infestans, a pathogen causing late blight disease...
  98. ncbi The first ankyrin-like repeat is the minimum indispensable key structure for functional assembly of homo- and heteromeric TRPC4/TRPC5 channels
    Rainer Schindl
    Institute for Biophysics, University of Linz, A 4040 Linz, Austria
    Cell Calcium 43:260-9. 2008
    ..Moreover a TRPC5 mutant that lacked the first ankyrin-like repeat was unable to homo-multimerise, failed to interact with wild-type TRPC5 and resulted in non-functional channels...
  99. pmc Folding of a designed simple ankyrin repeat protein
    V Sathya Devi
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Protein Sci 13:2864-70. 2004
    ..This work demonstrates that a designed minimal ankyrin repeat protein has the thermodynamic and kinetic properties of a compactly folded protein, and explains the favorable ..
  100. ncbi Differential inhibition of BmRelish1-dependent transcription in lepidopteran cells by bracovirus ankyrin-repeat proteins
    Christiana Magkrioti
    Insect Molecular Genetics and Biotechnology Group, Institute of Biology, National Centre for Scientific Research Demokritos, Neapoleos and Patr Grigoriou, 153 10 Aghia Paraskevi Attikis, Athens, Greece
    Insect Biochem Mol Biol 41:993-1002. 2011
    ..Thus, our results suggest that functional and spatial differences among the various CcBV Ank family members may be responsible for the observed differential inhibition of R1d2 activity...
  101. ncbi Cactus-independent nuclear translocation of Drosophila RELISH
    W D Cornwell
    Department of Gene Expression Sciences, GlaxoSmithKline Pharmaceuticals, King of Prussia, Pennsylvania, USA
    J Cell Biochem 82:22-37. 2001
    ....

Research Grants54

  1. Distinct roles of CARP target genes in wound healing
    Karinna Almodóvar; Fiscal Year: 2013
    ..Our lab has discovered a novel role of the nuclear transcription co-factor, cardiac ankyrin repeat protein (CARP), in the formation and abundance of new blood vessels during wound healing...
  2. MURINE TYPHUS: VECTOR BIOLOGY AND TRANSMISSION
    Abdu F Azad; Fiscal Year: 2013
    ..Based on our published work and preliminary data, we will target R. typhi surface cell antigens, ankyrin repeat proteins and patatin-like phospholipases...
  3. Orientia tsutsugamushi modulation of host cell ubiquitination machinery
    Jason A Carlyon; Fiscal Year: 2013
    ..The ankyrin repeat is the most common protein-protein interaction motif in nature...
  4. Asb2 in CD4+ T cell lineage differentiation and its plasticity
    Xiao Hong Sun; Fiscal Year: 2013
    ..Fresh ideas will likely emerge from these studies that will be o therapeutic value in the contest of immunity and autoimmunity. ..
  5. Obesity is a Surgical Problem
    Michael W Mulholland; Fiscal Year: 2013
    ..A continuing analysis of hypothalamic gene expression changes has identified ankyrin repeat and SOCS box containing protein 4 (Asb-4) as a very promising investigative target...
  6. Functional characterization of Orientia tsutsugamushi ankryin repeat proteins
    Jason A Carlyon; Fiscal Year: 2013
    ..Because Ank1, Ank5, and Ank17 carry both ankyrin repeat and coiled-coil motifs, we will include them in our functional studies...
  7. Molecular basis of ILK-mediated cell adhesion
    Jun Qin; Fiscal Year: 2013
    ..therapy? In preliminary studies, we found that ILK utilizes two distinct interfaces, one at its N-terminal ankyrin repeat domain (ARD) and the other at the pseudoactive site of the C-terminal kinase-like domain (KLD), to bind ..
  8. Cellular Physiology of Sensory Ion Channels
    David Julius; Fiscal Year: 2013
    ..Together, these studies will provide a rational basis for the development and design of novel analgesic agents for controlling chronic pain and neurogenic inflammatory syndromes. ..
  9. Filamin interactions in differentiation, invasion and disease
    David A Calderwood; Fiscal Year: 2013
    ..How filamin controls ECM degradation and invasion is unknown. In addition, we have shown that ASB2 (ankyrin repeat-containing protein with a suppressor of cytokine signaling box 2), part of an E3 ubiquitin ligase complex, ..
  10. Technology Development for Recombinant Affinity Reagents
    BRIAN KENNETH KAY; Fiscal Year: 2013
    ..types of recombinant affinity reagents: human single-chain Fragments of variable regions (scFv), Designed Ankyrin Repeat Proteins (DARPins), and fibronectin type III (FN3) monobodies. Dr...
  11. Enzyme-delivery scaffold technology for targeted cancer killing.
    Arnon Lavie; Fiscal Year: 2013
    ..modules will be based on the molecular scaffolds of single chain variable fragments (scFvs;~25 kDa), designed ankyrin repeat proteins (DARPins;~15 kDa), and affibodies (~ 9 kDa)...
  12. Inherited Dysfunction of Stretch Based Signaling in Dilated Cardiomyopathy
    Mousumi Moulik; Fiscal Year: 2012
    ..The PI has identified ANKRD1, as a novel DCM associated gene. ANKRD1 encodes for Cardiac Ankyrin Repeat Protein, a transcription-co-inhibitor which is a member of the Titin-N2A mechanosensory complex and is ..
  13. TRPV4: Mechanosensitivity and Skeletal Dysplasia
    Ching Kung; Fiscal Year: 2013
    ..This work aims towards understanding how ARD mutations can lead to two disparate diseases. In collaboration, we will also use trpV4-/- knock-out mice to test the role of TRPV4 in fracture healing. ..
  14. De novo engineering of protein agonists and antagonists
    CASIM SARKAR; Fiscal Year: 2013
    ..Here, we propose to exploit the modular fold of an alternative scaffold - the designed ankyrin repeat protein - to develop novel directed evolution approaches for isolating new agonists and antagonists...
  15. Molecular Basis of Pathogen-Induced Cell Death in Plants
    JEAN GREENBERG; Fiscal Year: 2009
    ..ACD6 is a novel integral plasma membrane protein with an N- terminal ankyrin repeat domain that controls defense and PCD in plants...
  16. HIV-envelope-specific DARPin-based microbicide strategies
    Alexandra Trkola; Fiscal Year: 2012
    ..collaboration, we identified a novel, highly specific approach to inhibit HIV infection using the Designed Ankyrin Repeat Protein (DARPin) technology...
  17. Modulation of Host Cell Functions by Coxiella burnetii
    Craig R Roy; Fiscal Year: 2010
    ..These studies will elucidate pathogenic determinants that allow host cell infection by Coxiella and reveal intracellular infection strategies employed by this important pathogen. ..
  18. NMR Studies or I-kappa B alpha and its interaction with NF-kappa B
    Carla Cervantes; Fiscal Year: 2009
    ..Mutations that restore the consensus sequence for stable ankyrin repeat proteins increase stability by 5 kcal/mol over wild type...
  19. Oncogenic Notch Signaling: Structural Studies
    STEPHEN BLACKLOW; Fiscal Year: 2006
    ..Solve the structure of a Notch transcriptional activation complex or a key subcomplex containing the Notch ankyrin repeat domain. [unreadable] [unreadable] 3B...
  20. Biacore T100
    John Carson; Fiscal Year: 2006
    ..Four major users have projects studying: molecular intractions in RNA trafficking, ankyrin repeat protein interactions, protein-protein interactions in secretory granule formation and molecular interactions ..
  21. ROLE OF TPL-2, CYCLIN D1 AND TOUVLO IN BREAST CANCER
    Christos Patriotis; Fiscal Year: 2002
    ..Both Tpl-2 and cyclin D1 interact with a novel ankyrin repeat protein, Touvlo. Touvlo interacts with the N-terminus of Tpl-2 and appears to regulate its kinase activity...
  22. SHARK KINASE SIGNALING IN THE ECOTDERMAL EPITHELIUM
    E Stanley; Fiscal Year: 2000
    ..We have termed this kinase Shark (SH2 domain ankyrin repeat kinase)...
  23. Folding and Assembly of Ankyrin Repeat Proteins
    Jeffrey Hoch; Fiscal Year: 2006
    ..Among all internal repeat proteins, those containing the ankyrin repeat, a 33-residue sequence motif, form one of the best-characterized and largest families...
  24. ROLE OF TVI 1 IN APOPTOSIS
    Philip Tsichlis; Fiscal Year: 2004
    DESCRIPTION: (Adapted from the investigator's abstract) Tvl-1, a 269 amino acid ankyrin repeat protein, was identified by screening a murine T-cell two hybrid cDNA library for proteins that associate with Raf-1...
  25. Structure-Function Relationship of Tumor Suppressors
    Junan Li; Fiscal Year: 2009
    ..abstract_text> ..
  26. Characterization of mutants affected in innate immunity
    Katrina Ramonell; Fiscal Year: 2005
    ..This data will ultimately shed more light on the mechanisms behind innate immunity in both animal and plant systems. ..
  27. Strategies for Selective Covalent Conjugation of Proteins with Small Molecules
    Fujie Tanaka; Fiscal Year: 2007
    ..compounds will be generated using reaction-based selections from combinatorial libraries of antibodies, ankyrin repeat proteins, and other proteins and peptides...
  28. Engineering membrane proteins for biophysical studies
    CASIM SARKAR; Fiscal Year: 2005
    ..This work will have a wide-reaching impact, ranging from fundamental studies on receptor biology and signal transduction to clinical applications in structure-based drug design. ..
  29. CONTROL OF BUD EMERGENCE IN YEAST
    Alan Bender; Fiscal Year: 1999
    ..IV. Immunolocalize Boi1, Bem4, and Rho3, and investigate the mechanisms by which Bem1, Boi1, and Rho3 become properly localized. V. Screen for genes whose products link the actions of Boi1/Boi2 and Rho3. ..
  30. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  31. Ehrilchiacidal Mechanisms In Leukocytes
    Yasuko Rikihisa; Fiscal Year: 2009
    ..The results may point to a potential target for new treatment and prevention of HME and HGE. ..
  32. GENE EXPRESSION BY HUMAN GRANULOCYTIC EHRLICHIOSIS AG
    Yasuko Rikihisa; Fiscal Year: 2004
    ..These efforts are expected to reveal an important role for the p44-multigene family in strategies of adaptive surface variation and avoidance of host immune responses. ..
  33. Acquisition of an MALDI TOF-TOF Mass Spectrometer
    ELIZABETH KOMIVES; Fiscal Year: 2005
    ..For this project, the MALDI-TOF-TOF will enable measurements of amide deuteration down to the single amino acid level. ..
  34. 2D Nano-flow Mass Spectrometry System
    ELIZABETH KOMIVES; Fiscal Year: 2006
    ..Hook and D. T. O'Connor will analyze the biosynthesis of bioactive peptides. Professor R. Tsien will explore the possibilities of using ReAsH reagents to discover proteins in close proximity in the cell. [unreadable] [unreadable]..
  35. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  36. BIOPHYSICAL STUDIES OF LRP LIGANDS INTERACTIONS
    ELIZABETH KOMIVES; Fiscal Year: 2003
    ..In particular, we will probe the role of hepara sulfate proteoglycans in the reaction. (5) Determine the structures of minimal fragments of LRP ligands bound to minimal fragments of the LRP extracellular domain. ..
  37. Directed Proteomics of Alzheimer's Related Proteins
    ELIZABETH KOMIVES; Fiscal Year: 2008
    ..4) Quantitative proteomics (ICAT) will be applied to age-correlated, AD and healthy brain lysates to develop a correlation between amounts of APP and LRP 1-protein interaction complexes and Abeta production. ..
  38. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY YW BOURGUIGNON; Fiscal Year: 2010
    ....
  39. ID OF THE MOUSE DEAFNESS (DN) GENE ON CHROMOSOME 19
    Bronya Keats; Fiscal Year: 2002
    ..The deafness mouse is a model for nonsyndromic profound hearing impairment, and identifying the defective gene will be a valuable contribution to our understanding of the genes needed for normal cochlear function. ..
  40. The Johns Hopkins Folding Meeting
    DOUGLAS BARRICK; Fiscal Year: 2005
    ..Participants will also be selected to provide a balance in gender and career level. ..
  41. Microfilaments in Giardia Attachment and Virulence
    HEIDI ELMENDORF; Fiscal Year: 2006
    ..This work will advance our understanding of parasite pathogenesis and develop our knowledge of microfilaments as a target for drug design. ..
  42. MOLECULAR MECHANISMS OF NEURONAL CELL MIGRATION
    Richard Cameron; Fiscal Year: 2005
    ....
  43. Plasticity and learning in a corticostriatal network
    Ann Kelley; Fiscal Year: 2006
    ..Using anatomical, molecular, and behavioral approaches, we will test the hypothesis that NMDA- and Dl-receptor mediated plasticity within multiple nodes of a neural network controls new adaptive motor learning. ..
  44. TRPM4 Channels in the Control of Cerebral Artery Tone
    Scott Earley; Fiscal Year: 2005
    ..It is anticipated that these studies will generate significant new information regarding the role of mechanosensitive cation channels in the regulation of VSM membrane potential and the regulation of cerebral blood flow. ..
  45. Transcriptional Regulation in Giardia lamblia
    HEIDI ELMENDORF; Fiscal Year: 2006
    ..These studies will build an understanding of transcription in Giardia lamblia and lay foundations for study of the role of the regulation of gene expression in disease. ..
  46. T CELL ADJUVANTS FOR BIODEFENSE
    Thomas Mitchell; Fiscal Year: 2008
    ..External salary support will lessen the load of non-research duties, such that conceptual advances in vaccine design for biodefense can be made. ..
  47. Bcl-3 Oncoprotein in T Cell Survival
    Thomas Mitchell; Fiscal Year: 2006
    ..Experiments with these mice will identify those adjuvant effects on T cells for which Bcl-3 expression is required and which could therefore be targeted by drug inhibition. ..
  48. PKC-EHN-PKD signal complex in cardiac remodeling
    Masahiko Hoshijima; Fiscal Year: 2009
    ..abstract_text> ..
  49. Plasticity and learning in a corticostriatal network
    Matthew Andrzejewski; Fiscal Year: 2007
    ..Using anatomical, molecular, and behavioral approaches, we will test the hypothesis that NMDA- and Dl-receptor mediated plasticity within multiple nodes of a neural network controls new adaptive motor learning. ..
  50. Pathogenesis of Notch Leukemia
    Jon Aster; Fiscal Year: 2007
    ..1. To identify domains of MAML1 that are essential for function in normal and transformed lymphoid cells 3.2. To identify and characterize small molecule inhibitors of CSL/ICN/MAML1 function ..
  51. Cryptosporidium parvum Histone Deacetylases
    STANLEY RIDER; Fiscal Year: 2007
    ..There are currently no highly effective treatments for Cryptosporidium parvum. This research is a beginning step toward identifying novel methods to control this parasite. [unreadable] [unreadable] [unreadable]..
  52. Gene-Environment Interplay in Adolescent Alcohol Use
    Danielle M Dick; Fiscal Year: 2010
    ..abstract_text> ..
  53. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..