Genomes and Genes
fluorescent antibody technique
Summary: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Publications417 found, 100 shown here
- The small GTP-binding protein rac regulates growth factor-induced membrane rufflingA J Ridley
Institute for Cancer Research, Chester Beatty Laboratories, London, England
Cell 70:401-10. 1992..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
- Identification and characterization of ovarian cancer-initiating cells from primary human tumorsShu Zhang
Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana 47405, USA
Cancer Res 68:4311-20. 2008..Based on these findings, we assert that epithelial ovarian cancers derive from a subpopulation of CD44(+)CD117(+) cells, thus representing a possible therapeutic target for this devastating disease...
- Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopyLaurentiu M Popescu
Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
Cell Tissue Res 345:391-403. 2011..In conclusion, this study shows the presence of TCs in lungs and identifies a TC-SC tandem in subepithelial niches of the bronchiolar tree. In TC-SCNs, the synergy of TCs and SCs may be based on nanocontacts and shed vesicles...
- Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitorNoboru Sato
Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Nat Med 10:55-63. 2004..These results suggest that the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine...
- Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuitsKristina D Micheva
Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
Neuron 55:25-36. 2007..The application of array tomography can reveal important but previously unseen features of brain molecular architecture...
- Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homologD L Pittman
Jackson Laboratory, Bar Harbor, Maine 04609, USA
Mol Cell 1:697-705. 1998..The strong similarity of phenotypes in Dmc1-deficient mice and yeast suggests that meiotic mechanisms have been highly conserved through evolution...
- Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stressK Stamer
Max Planck Unit for Structural Molecular Biology, 22607 Hamburg, Germany
J Cell Biol 156:1051-63. 2002..The data suggests a linkage between tau and APP trafficking, which may be significant in Alzheimer's disease...
- A major role for the Plasmodium falciparum ApiAP2 protein PfSIP2 in chromosome end biologyChristian Flueck
Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, University of Basel, Basel, Switzerland
PLoS Pathog 6:e1000784. 2010..These findings are highly relevant for our understanding of chromosome end biology and variegated expression in P. falciparum and other eukaryotes, and for the future analysis of the role of ApiAP2-DNA interactions in parasite biology...
- The genomic response to retinal disease and injury: evidence for endothelin signaling from photoreceptors to gliaAmir Rattner
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Neurosci 25:4540-9. 2005....
- An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transitionClaude C Warzecha
Renal Division, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
EMBO J 29:3286-300. 2010....
- An essential role for beta-actin mRNA localization and translation in Ca2+-dependent growth cone guidanceJiaqi Yao
Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
Nat Neurosci 9:1265-73. 2006....
- The nuclear protein HMGB1 is secreted by monocytes via a non-classical, vesicle-mediated secretory pathwayStefania Gardella
National Cancer Research Institute, Genoa, Italy
EMBO Rep 3:995-1001. 2002..Thus, in monocytes, non-classical secretion can occur through vescicle compartments that are at least partially distinct...
- Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondiiPeter J Bradley
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
J Biol Chem 280:34245-58. 2005....
- Chikungunya virus-induced autophagy delays caspase-dependent cell deathPierre Emmanuel Joubert
Unité Immunobiologie des Cellules Dendritiques, Department of Immunology, Institut Pasteur, Paris, France
J Exp Med 209:1029-47. 2012..Our study suggests that inducers of autophagy may limit the pathogenesis of acute Chikungunya disease...
- Nuclear localization and regulation of erk- and rsk-encoded protein kinasesR H Chen
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115
Mol Cell Biol 12:915-27. 1992....
- Staphylococcus aureus-derived peptidoglycan induces Cx43 expression and functional gap junction intercellular communication in microgliaSarita Garg
Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
J Neurochem 95:475-83. 2005....
- FGF9 suppresses meiosis and promotes male germ cell fate in miceJosephine Bowles
Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
Dev Cell 19:440-9. 2010....
- Characterization of a cis-Golgi matrix protein, GM130N Nakamura
Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
J Cell Biol 131:1715-26. 1995..Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure...
- Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3H Saitoh
The Picower Institute for Medical Research, Manhasset, New York 11030, USA
J Biol Chem 275:6252-8. 2000..Together, these results support the concept of important distinctions between the SUMO-2/3 and SUMO-1 conjugation pathways and suggest a role for SUMO-2/3 in the cellular responses to environmental stress...
- Transient expression of doublecortin during adult neurogenesisJason P Brown
Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
J Comp Neurol 467:1-10. 2003..This assumption is further supported by our observation that the number of DCX-expressing cells in the dentate gyrus was decreased with age according to the reduction of neurogenesis in the aging dentate gyrus previously reported...
- Directed differentiation of telencephalic precursors from embryonic stem cellsKiichi Watanabe
Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
Nat Neurosci 8:288-96. 2005..Thus, in the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals...
- Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleusX D Fu
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
Nature 343:437-41. 1990..These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus...
- Export of a Toxoplasma gondii rhoptry neck protein complex at the host cell membrane to form the moving junction during invasionSebastien Besteiro
UMR 5235 CNRS, Université de Montpellier 2, Montpelier, France
PLoS Pathog 5:e1000309. 2009..Globally, these results point toward a model of MJ organization where the parasite would be secreting and inserting interacting components on either side of the MJ, both at the host and at its own plasma membranes...
- The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochoresRam Kumar Mishra
Laboratory of Gene Regulation and Development, NICHD NIH, Bethesda, MD 20892, USA
Nat Cell Biol 12:164-9. 2010....
- Aggregation of huntingtin in neuronal intranuclear inclusions and dystrophic neurites in brainM Difiglia
Department of Neurology, Massachusetts General Hospital, Boston, MA 02114, USA
Science 277:1990-3. 1997..Ubiquitin was also found in NIIs and DNs, which suggests that abnormal huntingtin is targeted for proteolysis but is resistant to removal. The aggregation of mutant huntingtin may be part of the pathogenic mechanism in HD...
- mDia mediates Rho-regulated formation and orientation of stable microtubulesA F Palazzo
Department of Anatomy and Cell Biology, Columbia University, New York 10032, USA
Nat Cell Biol 3:723-9. 2001..Our results show that mDia is sufficient to generate and orient stable microtubules, and indicate that Dia-related formins are part of a conserved pathway that regulates the dynamics of microtubule ends...
- Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndromeMaria Eriksson
Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314, USA
Nature 423:293-8. 2003..The discovery of the molecular basis of this disease may shed light on the general phenomenon of human ageing...
- Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation methodChristopher J Tonkin
Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Parkville, VIC 3010, Australia
Mol Biochem Parasitol 137:13-21. 2004..falciparum proteins...
- Signal recognition particle components in the nucleolusJ C Politz
Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 97:55-60. 2000..Our findings support the notion that the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis, in addition to ribosomes themselves...
- Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell lineJ V Kilmartin
J Cell Biol 93:576-82. 1982..The purified antibodies could be conjugated to colloidal gold particles and used for direct labeling of yeast microtubules for electron microscopy...
- Focal adhesion disassembly requires clathrin-dependent endocytosis of integrinsWei Ting Chao
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 1 Baylor Plaza, BCM335, RM T419, Houston, TX 77030, USA
FEBS Lett 583:1337-43. 2009..Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway...
- Mandibuloacral dysplasia is caused by a mutation in LMNA-encoding lamin A/CGiuseppe Novelli
Department of Biopathology and Diagnostic Imaging, Faculty for Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133 Romea, Italy
Am J Hum Genet 71:426-31. 2002..Patient skin fibroblasts showed nuclei that presented abnormal lamin A/C distribution and a dysmorphic envelope, thus demonstrating the pathogenic effect of the R527H LMNA mutation...
- Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localizationJ A Diehl
Howard Hughes Medical Institute, St Jude Children s Research Hospital, Memphis, Tennessee 38105 USA
Genes Dev 12:3499-511. 1998..Therefore, phosphorylation and proteolytic turnover of cyclin D1 and its subcellular localization during the cell division cycle are linked through the action of GSK-3beta...
- Neuregulin-1 type III determines the ensheathment fate of axonsCarla Taveggia
Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
Neuron 47:681-94. 2005..These results indicate that levels of NRG1 type III, independent of axon diameter, provide a key instructive signal that determines the ensheathment fate of axons...
- Antigenicity and immunogenicity of a novel chimeric peptide antigen based on the P. vivax circumsporozoite proteinNora Céspedes
Malaria Vaccine and Drug Development Center MVDC, Carrera 37 2Bis No 5E 08, A A 25574 Cali, Colombia Biochemistry Department, University of Lausanne, 155 ch des Boveresses, 1066 Epalinges, Lausanne, Switzerland Faculty of Health, University of Valle, Calle 4B No 36 00 Cali, Colombia
Vaccine 31:4923-30. 2013..PvCS contains a dimorphic central repetitive fragment flanked by conserved regions that contain functional domains...
- Production of melanin by Aspergillus fumigatusSirida Youngchim
Dermatology Department, St Johns Institute of Dermatology, Guy s Hospital, Kings and St Thomas Medical Schools, London, UK
J Med Microbiol 53:175-81. 2004..Melanin-binding mAbs were used to detect the presence of melanized conidia in three patients with nasal aspergilloma, indicating that in vivo melanization may occur during infection...
- CD133, a novel marker for human prostatic epithelial stem cellsGavin D Richardson
Prostate Research Group, Surgical Oncology, Medical School, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
J Cell Sci 117:3539-45. 2004..alpha(2)beta(1)(hi)/CD133(+) cells exhibit two important attributes of epithelial stem cells: they possess a high in vitro proliferative potential and can reconstitute prostatic-like acini in immunocompromised male nude mice...
- Mouse very long-chain Acyl-CoA synthetase 3/fatty acid transport protein 3 catalyzes fatty acid activation but not fatty acid transport in MA-10 cellsZhengtong Pei
Kennedy Krieger Research Institute and Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 279:54454-62. 2004..These studies cast doubt on the designation of ACSVL3 as a fatty acid transport protein...
- The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasisElisabeth Raschperger
Ludwig Institute for Cancer Research, Nobels v 3, SE 171 77 Stockholm, Sweden
Exp Cell Res 312:1566-80. 2006..We conclude that CAR is localized to epithelial tight junctions in vivo where it may play a role in the regulation of epithelial permeability and tissue homeostasis...
- Evidence for transcriptional activity in the syncytiotrophoblast of the human placentaP M Ellery
Centre for Trophoblast Research, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK
Placenta 30:329-34. 2009..Contrary to previous findings, these results confirm that a proportion of syncytiotrophoblast nuclei actively produce mRNA transcripts...
- Early exercise promotes positive hippocampal plasticity and improves spatial memory in the adult life of ratsSergio Gomes da Silva
Department of Physiology, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
Hippocampus 22:347-58. 2012..Our findings demonstrate that physical exercise during postnatal development results in positive changes for the hippocampal formation, both in structure and function...
- mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndromeNoriko Ito
Department of Pediatrics, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Lab Invest 91:1584-95. 2011..In conclusion, mTORC1 activation may perturb the regulatory system of energy metabolism primarily by promoting energy consumption and inducing the UPR, which underlie proteinuria in MCD...
- RNA interference targeting survivin exerts antitumoral effects in vitro and in established glioma xenografts in vivoSandy Hendruschk
Department of Neurosurgery, University Hospital Carl Gustav Carus, Dresden, Germany
Neuro Oncol 13:1074-89. 2011..We conclude that survivin is functionally relevant in gliomas and that PEI-mediated exogenous delivery of siRNA targeting survivin is a promising strategy for glioblastoma therapy...
- Premature osteoblast clustering by enamel matrix proteins induces osteoblast differentiation through up-regulation of connexin 43 and N-cadherinRichard J Miron
Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
PLoS ONE 6:e23375. 2011..These findings provide further support for the clinical evidence that EMD increases the speed and quality of new bone formation in vivo...
- Chk2 deficiency in Myc overexpressing lymphoma cells elicits a synergistic lethal response in combination with PARP inhibitionAndreas Höglund
Department of Molecular Biology, Umea University, Umea, Sweden
Cell Cycle 10:3598-607. 2011..Our data indicates that only certain types of chemotherapy would give rise to a synergistic lethal response in combination with specific Chk2 inhibitors, which will be important if Chk2 inhibitors enter the clinic...
- CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosisH Liao
Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA
J Cell Biol 130:507-18. 1995..The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events...
- An essential cytoskeletal linker protein connecting actin microfilaments to intermediate filamentsY Yang
Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
Cell 86:655-65. 1996..In BPAG1 null mice, axonal architecture is markedly perturbed, consistent with a failure to tether neurofilaments to the actin cytoskeleton and underscoring the physiological relevance of this protein...
- Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the inhibitory function of SufuShu Hsuan C Hsu
Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada
Development 138:3791-801. 2011..Furthermore, Kif7 also inhibits Gli transcriptional activity in the chondrocytes when Sufu function is absent. Therefore, Kif7 regulates the activity of Gli transcription factors through both Sufu-dependent and -independent mechanisms...
- Onecut transcription factors are required for the second phase of development of the A13 dopaminergic nucleus in the mouseAgnès Espana
Universite Catholique de Louvain, Institute of Neuroscience, Laboratory of Neural Differentiation, Brussels, B 1200, Belgium
J Comp Neurol 520:1424-41. 2012..Hence, Onecut factors are markers of the A13 nucleus throughout embryonic development. They are dispensable for the first phase of A13 development but are required for the second phase of development and for maintenance of this nucleus...
- Feeder- and serum-free establishment and expansion of human induced pluripotent stem cellsMehdi Totonchi
Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Int J Dev Biol 54:877-86. 2010..Such hiPSCs could be useful particularly in the context of in vitro disease modeling, pharmaceutical screening and in cellular replacement therapies once the safety issues have been overcome...
- Intracerebroventricular infusion of monoclonal antibody or its derived Fab fragment against misfolded forms of SOD1 mutant delays mortality in a mouse model of ALSFrancois Gros-Louis
Department of Psychiatry and Neuroscience, Research Centre of CHUQ, Universite Laval, Quebec, QC, Canada
J Neurochem 113:1188-99. 2010..From these results, we propose that passive immunization strategies should be considered as potential avenues for treatment of familial amyotrophic lateral sclerosis caused by SOD1 mutations...
- Epigenetic modifications on X chromosomes in marsupial and monotreme mammals and implications for evolution of dosage compensationWillem Rens
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 OES, United Kingdom
Proc Natl Acad Sci U S A 107:17657-62. 2010..In comparison to the marsupial, the monotreme exhibited no epigenetic differences between homologous X chromosomes, suggesting the absence of a dosage compensation process comparable to that in therians...
- AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of RanvierE Kordeli
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710
J Biol Chem 270:2352-9. 1995..The neural-specific ankyrinG polypeptides are candidates to participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments...
- The mago nashi locus encodes an essential product required for germ plasm assembly in DrosophilaP A Newmark
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309
Development 120:1303-13. 1994..These results demonstrate that mago nashi encodes an essential product necessary for the localization of germ plasm components to the posterior pole of the oocyte...
- Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cellsN Zerangue
Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, CA 94143-0725, USA
Proc Natl Acad Sci U S A 98:2431-6. 2001..Finally, using sequences from our screen, we found that the potency of di-lysine, but not di-arginine, mediated endoplasmic reticulum localization was correlated with the strength of interaction with alpha-COP...
- Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM)W Baschong
M E Muller Institute at the Biozentrum, Aarau, Switzerland
J Histochem Cytochem 49:1565-72. 2001..Sudan Black B is suggested to mask fluorescent tissue components...
- Tissue-specific and inducible Cre-mediated recombination in the gut epitheliumFatima el Marjou
Morphogenesis and intracellular signalling, Institut Curie CNRS, Paris, France
Genesis 39:186-93. 2004..The villin-Cre and villin-Cre-ERT2 mice provide valuable tools for studies of cell lineage allocation and gene function in the developing and adult intestine...
- C-kit immunopositive interstitial cells (Cajal-type) in human myometriumSanda M Ciontea
Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, 050474, Romania
J Cell Mol Med 9:407-20. 2005..In conclusion, we describe myometrial Cajal-like interstitial cells that have affinity for methylene blue and Janus green B vital dyes, fulfill (all) TEM criteria, express CD117/c-kit and have spontaneous electric activity...
- The polo-like kinase 1 regulates CDC25B-dependent mitosis entryValerie Lobjois
Universite de Toulouse, LBCMCP, 118 route de Narbonne, CNRS, LBCMCP UMR5088, F 31062 Toulouse, France
Biochim Biophys Acta 1793:462-8. 2009..Our results support a model in which the relocalisation of CDC25B to the nucleus at the G2-M transition by PLK1 regulates its mitotic inducing activity...
- CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoproteinPaul Gutwein
Pharmazentrum Frankfurt, Klinikum der Johann Wolfgang Goethe Universitat Frankfurt, Theodor Stern Kai 7, D 60590 Frankfurt am Main, Germany
Am J Pathol 174:2061-72. 2009..In summary, we presume important roles for CXCL16, ADAM10, and ADAM17 in the development of MN, suggesting these proteins as new therapeutic targets in this glomerular kidney disease...
- Primary cilia disappear in rat podocytes during glomerular developmentKoichiro Ichimura
Department of Anatomy and Life Structure, Juntendo University School of Medicine, Tokyo, Japan
Cell Tissue Res 341:197-209. 2010..Thus, the disappearance of the primary cilia presumably prevents the entry of excessive calcium-ions via the cilium-associated polycystin complexes and the disturbance of intracellular signaling cascades in mature podocytes...
- Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathwayShan Shan Liu
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China
J Biomed Sci 18:58. 2011..In the present study, we investigated the effect of Rab5a on cervical cancer invasion and metastasis and the molecular mechanism underlying the involvement of Rab5a...
- SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membraneJian Huang
Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine SJTU SM, Shanghai 200025, China
Nat Commun 3:911. 2012..Our data demonstrate a molecular mechanism whereby SUMO1 modification is required for PTEN tumour suppressor function by controlling PTEN membrane association and regulation of the phosphatidylinositol-3 kinase/AKT pathway...
- Detection of Leishmania infantum in dogs by PCR with lymph node aspirates and bloodS Reale
Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy
J Clin Microbiol 37:2931-5. 1999..These animals could contribute to the spreading of infection among dogs, and represent a potential risk for human health as well...
- Wiskott-Aldrich syndrome protein regulates podosomes in primary human macrophagesS Linder
Max von Pettenkofer Institut für Medizinische Mikrobiologie, Pettenkoferstrasse 9a, Ludwig Maximilians Universitat, 80336 Munich, Germany
Proc Natl Acad Sci U S A 96:9648-53. 1999..These findings indicate that WASp controls podosome assembly and, in cooperation with CDC42Hs, podosome disassembly in primary human macrophages...
- Nucleolar assembly of the rRNA processing machinery in living cellsT M Savino
Institut Jacques Monod, UMR 7592, 2 place Jussieu, 75251 Paris Cedex 05, France
J Cell Biol 153:1097-110. 2001..This demonstrates the formation of one functional domain by cooperative interactions between different chromosome territories...
- DNA replication licensing and human cell proliferationK Stoeber
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
J Cell Sci 114:2027-41. 2001..We conclude that biomarkers for replication-licensed cells detect, in addition to actively proliferating cells, cells with growth potential, a concept that has major implications for developmental and cancer biology...
- The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependentEva Hannak
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
J Cell Biol 157:591-602. 2002..Thus, although unknown mechanisms can support partial assembly of mitotic centrosomal asters, gamma-tubulin is the kinetically dominant centrosomal MT nucleator...
- An epidemiological study of RSV infection in the GambiaMartin W Weber
Medical Research Council Laboratories, Fajara, The Gambia
Bull World Health Organ 80:562-8. 2002..To describe the epidemiology of respiratory syncytial virus (RSV) infection in a developing country...
- Phase segregation of medial septal GABAergic neurons during hippocampal theta activityZsolt Borhegyi
Department of Cellular and Network Neurobiology, Institute of Experimental Medicine of the Hungarian Academy of Science, Budapest 1083, Hungary
J Neurosci 24:8470-9. 2004....
- EMMPRIN-induced MMP-2 activation cascade in human cervical squamous cell carcinomaCornelis F M Sier
Department of Pathology, Leiden University Medical Center, The Netherlands
Int J Cancer 118:2991-8. 2006....
- TWEAK-Fn14 pathway inhibition protects the integrity of the neurovascular unit during cerebral ischemiaXiaohui Zhang
Department of Neurology and Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA
J Cereb Blood Flow Metab 27:534-44. 2007..These findings show that the cytokine TWEAK plays a role in the disruption of the structure of the NVU during cerebral ischemia and that TWEAK antagonism is a potential therapeutic strategy for acute cerebral ischemia...
- Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophinBrian M Burkel
Department of Zoology, University of Wisconsin Madison, Madison, Wisconsin, USA
Cell Motil Cytoskeleton 64:822-32. 2007....
- Bipolar, Dual Plasmodium falciparum helicase 45 expressed in the intraerythrocytic developmental cycle is required for parasite growthArun Pradhan
Malaria Group, International Centre for Genetic Engineering and Biotechnology, PO Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India
J Mol Biol 373:268-81. 2007..These studies indicate that PfH45 is an indispensable enzyme that is essential for growth, and probably survival, of P. falciparum...
- Gene regulation logic in retinal ganglion cell development: Isl1 defines a critical branch distinct from but overlapping with Pou4f2Xiuqian Mu
Department of Biochemistry and Molecular Biology, M D Anderson Cancer Center and Graduate Training Program in Genes and Development, Graduate School of Biomedical Sciences, University of Texas, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 105:6942-7. 2008..They also reveal that identical RGC expression patterns are achieved by different combinations of divergent inputs from upstream transcription factors...
- Quantitative analysis of changes in ER, PR and HER2 expression in primary breast cancer and paired nodal metastasesS J Aitken
Division of Pathology, University of Edinburgh, Edinburgh, UK
Ann Oncol 21:1254-61. 2010..Since de novo resistance to therapy is common, we quantified differences in receptor expression between primary and nodal disease in order to assess whether this might contribute to therapeutic resistance...
- A bicomponent Plasmodium falciparum investigational vaccine composed of protein-peptide conjugatesJoanna Kubler-Kielb
Program on Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:1172-7. 2010....
- Symplekin promotes tumorigenicity by up-regulating claudin-2 expressionMichael Buchert
Institut de Genomique Fonctionnelle, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5203, Institut National de la Sante et de la Recherche Medicale U661, and Université Montpellier 1 and 2, Montpellier F 34094, France
Proc Natl Acad Sci U S A 107:2628-33. 2010..Our results suggest that nuclear overexpression of symplekin promotes tumorigenesis in the human colon and that the regulation of claudin-2 expression is instrumental in this effect...
- Compartmentalized organization: a common and required feature of stem cell niches?Valentina Greco
Department of Genetics, Yale University School of Medicine and Yale Stem Cell Center, SHM I 141A, New Haven, CT 06510, USA
Development 137:1586-94. 2010....
- Human cytomegalovirus UL29/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNAScott S Terhune
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
PLoS Pathog 6:e1000965. 2010..We propose that pUL29/28 modifies the NuRD complex to stimulate the accumulation of immediate-early RNAs...
- In vivo formation of gamma-H2AX and 53BP1 DNA repair foci in blood cells after radioiodine therapy of differentiated thyroid cancerMichael Lassmann
Department of Nuclear Medicine, University of Wurzburg, Wurzburg, Germany
J Nucl Med 51:1318-25. 2010....
- CIP2A expression and localization in oral carcinoma and dysplasiaJoseph Katz
Department of Oral Maxillofacial Surgery and Diagnostic Sciences, University of Florida, College of Dentistry, FL, USA
Cancer Biol Ther 10:694-9. 2010..This study was designed to investigate the expression of CIP2A in OSCC cell lines and tissues representing human normal, dysplasia and OSCC...
- Synaptic vesicle-like lipidome of human cytomegalovirus virions reveals a role for SNARE machinery in virion egressSean T H Liu
Department of Molecular Biology, Lewis Thomas Laboratory, University, Princeton, NJ 08544, USA
Proc Natl Acad Sci U S A 108:12869-74. 2011..We propose that cytomegalovirus capsids acquire their envelope by budding into vesicles with a lipid composition similar to that of synaptic vesicles, which subsequently fuse with the plasma membrane to release virions from the cell...
- Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermisKaelyn D Sumigray
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
J Cell Biol 194:631-42. 2011..This work reveals essential desmosome-associated components that control cortical microtubule organization and unexpected roles for centrosomal proteins in epidermal function...
- Sequential assembly of the nucleotide excision repair factors in vivoM Volker
Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands
Mol Cell 8:213-24. 2001....
- Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAPShin Ichi Kawaguchi
Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA
Mol Biol Cell 15:37-45. 2004..These findings suggest that the microtubule-nucleating activity of the centrosome requires the function of CP309...
- N-cadherin is dispensable for pancreas development but required for beta-cell granule turnoverJenny K Johansson
Stem Cell Center, Department of Laboratory Medicine, Lund University, Sweden
Genesis 48:374-81. 2010..The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased...
- Increased disease severity of parasite-infected TLR2-/- mice is correlated with decreased central nervous system inflammation and reduced numbers of cells with alternatively activated macrophage phenotypes in a murine model of neurocysticercosisUma Mahesh Gundra
Department of Biology, South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, One UTSA Circle, San Antonio, Texas 78249 1644, USA
Infect Immun 79:2586-96. 2011..Importantly, these results demonstrate that TLR2-associated responses modulate the disease severity of murine NCC...
- The making of a gradient: IcsA (VirG) polarity in Shigella flexneriJ R Robbins
Department of Biochemistry, Stanford University School of Medicine, 279 West Campus Drive, Stanford, CA 94305-5307, USA
Mol Microbiol 41:861-72. 2001....
- Expression and localisation of GLUT1 and GLUT12 glucose transporters in the pregnant and lactating rat mammary glandMaria L Macheda
Department of Medicine, St Vincent s Hospital, University of Melbourne, PO Box 2900, Fitzroy, Victoria 3065, Australia
Cell Tissue Res 311:91-7. 2003..Forced weaning resulted in decreased cytoplasmic GLUT1 staining intensity, but no change in GLUT12 staining. The results suggest a possible role for GLUT12 in the metabolism of mammary epithelial cells during pregnancy and lactation...
- Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneriRenato Morona
Department of Molecular and Biomedical Science, University of Adelaide, SA, Australia
FEMS Microbiol Lett 221:173-80. 2003..These results have implications for the mechanism of IcsA export. They suggest that while IcsA export is predominantly targeted to the old cell pole, it can also occur on the lateral regions of the cell surface...
- Human airway epithelial cell culture to identify new respiratory viruses: coronavirus NL63 as a modelBridget S Banach
Department of Microbiology and Immunology, Loyola University Chicago Stritch School of Medicine, 2160 South First Avenue, Maywood, IL, USA
J Virol Methods 156:19-26. 2009....
- N-WASp is required for Schwann cell cytoskeletal dynamics, normal myelin gene expression and peripheral nerve myelinationFuzi Jin
Department of Haematology, Xijing Hospital, Fourth Military Medical University, Xi an, China
Development 138:1329-37. 2011..Our observations demonstrate that N-WASp plays an essential role in Schwann cell maturation and myelin formation...
- Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray dosesKai Rothkamm
Fachrichtung Biophysik, Universitat des Saarlandes, 66421 Homburg, Germany
Proc Natl Acad Sci U S A 100:5057-62. 2003....
- Implications of cytokeratin 8/18 filament formation in stratified epithelial cells: induction of transformed phenotypeUjwala Raul
Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Cancer Research Institute, Kharghar, Navi Mumbai, India
Int J Cancer 111:662-8. 2004..In both cases, lung metastasis was also seen. Thus, in conclusion, it appears that increased expression of CK8 in some way changes the phenotypic characteristics of stratified epithelial cells, resulting in malignant transformation...
- Angiotensin AT2 receptor protects against cerebral ischemia-induced neuronal injuryJun Li
Center for Cardiovascular Research Institute of Pharmacology and Toxicology, Charite University Medicine Berlin, Germany
FASEB J 19:617-9. 2005..Our data indicate that cerebral AT2 receptors exert neuroprotective actions in response to ischemia-induced neuronal injury, possibly by supporting neuronal survival and neurite outgrowth in peri-ischemic brain areas...
- Expression and modulation of an NADPH oxidase in mammalian astrocytesAndrey Y Abramov
Department of Physiology, University College London, London WC1E 6BT, United Kingdom
J Neurosci 25:9176-84. 2005..The astrocytic NADPH oxidase is likely to play important roles in CNS physiology and pathology...
- Myt1 and Ngn3 form a feed-forward expression loop to promote endocrine islet cell differentiationSui Wang
Program in Developmental Biology, Department of Cell and Developmental Biology, 465 21st Avenue South, Rm 4128, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Dev Biol 317:531-40. 2008....
- Spatiotemporal regulation of early lipolytic signaling in adipocytesSally Martin
Division of Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia
J Biol Chem 284:32097-107. 2009..These results describe the remarkably rapid, sequential phosphorylation of specific serine residues in HSL at spatially distinct intracellular locales, providing new insight into the complex regulation of lipolysis...
- Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in the transition from colitis to cancerJoseph E Carpentino
McKnight Brain Institute, University of Florida, Gainesville, Florida 32609, USA
Cancer Res 69:8208-15. 2009..These data link colitis and cancer identifying potential tumor-initiating cells from colitic patients, suggesting that sphere and/or xenograft formation will be useful to survey colitic patients at risk of developing cancer...
- Ubiquitin ligase cullin 7 induces epithelial-mesenchymal transition in human choriocarcinoma cellsJiejun Fu
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
J Biol Chem 285:10870-9. 2010..These results suggest that the CUL7 E3 ligase is a key regulator in trophoblast cell epithelial-mesenchymal transition and placental development...
- Inhibition of human neutrophil function by Francisella tularensisLee Ann H Allen; Fiscal Year: 2012..The results of this study may lead to new treatments to combat this severe and often fatal lung infection. ..
- Bradley P Dixon; Fiscal Year: 2014..This may lead to cancer of the bladder, especially in children who have their bladders surgically reconstructed. ..
- Rac GTPase signaling during embryonic salivary gland branching morphogenesisSharon Sequeira; Fiscal Year: 2012....
- Samuel D Crish; Fiscal Year: 2016..This proposal is designed with the goal of identifying new therapeutic targets for this disease as well as identifying the potential role that function-restoring therapies could play in glaucoma and other neurodegenerative diseases. ..
- Novel Peptide MPO Inhibitors for Treating AtherosclerosisKirkwood A Pritchard; Fiscal Year: 2012..As these disease states represent a major focus of the NIH, findings from our proposal will advance the research and mission of several different institutes. ..
- ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2014..The work is clinically relevant because it will resolve questions about iron in the formal pathogenesis and natural history of FRDA, and the potential value of iron chelation. ..
- Cell signaling as a leukemia biomarkerJAMES WILLIAM JACOBBERGER; Fiscal Year: 2013....
- Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule CellDIANA LYNNE GOLDEN; Fiscal Year: 2012..Extensive examination of the mechanisms and potential therapeutic targets will advance the understanding and potential cure for AKI in proximal tubular regeneration. ..
- Phillip Darwin Bell; Fiscal Year: 2016..Thus there is a compelling reason to focus on this disease and its effective treatment or cure, which would greatly benefit the VA patient population. ..
- RSK-2 regulates integrin-mediated adhesion and migrationJOE WILLIAM RAMOS; Fiscal Year: 2013..This will determine if these proteins are suitable targets for the development of new adhesion specific disease therapies. In particular we will test if RSK2 can serve as a potential new therapeutic target in glioma. ..
- Role of murine induced pluripotent stem cells on the correction of cardiac and skDiego Fraidenraich; Fiscal Year: 2010..These experiments will help elucidate the molecular mechanisms that the iPS cells may utilize to effect corrections in cardiac and skeletal muscle and will broaden the therapeutic applicability of the iPS cells. ..
- AKAP Regulation of PKA Targeting in the HeartMeredith Bond; Fiscal Year: 2009..Our project investigates a novel nuclear AKAP protein, Chd8 that may participate in regulation of cardiac remodeling during ischemic heart failure. ..
- Connexins in Neuronal and Glial Gap Junctions in the Central Nervous SystemJohn E Rash; Fiscal Year: 2012..normal human cerebral cortex and hippocampus. These data will serve as a prelude to measuring changes in these structures in human disease such as epilepsy, schizophrenia, Parkinson's disease, and autism spectral disorders. ..
- Regulation of the Endothelial Citrulline-Nitric Oxide CycleDuane C Eichler; Fiscal Year: 2010..enzyme that is required to maintain vascular wall function is essential to this understanding, and will potentially distinguish new therapeutic strategies for the treatment of diabetes, hypertension and related cardiovascular disease ..
- Jessica B Kendrick; Fiscal Year: 2015..Furthermore, clinical and in vitro methods will be used to identify the mechanisms by which vitamin D affects vascular endothelial function, which will enhance our understanding of the non-calcemic effects of vitamin D. ..
- Thiol Isomerases During Thrombus FormationBarbara C Furie; Fiscal Year: 2013..abstract_text> ..
- Is symmetrical dimethylation of Arginine 2 of Histone H3 used as an epigenetic siMarjorie A Oettinger; Fiscal Year: 2010..The experiments proposed here should define the roles of this modification and lead to insights into its possible role in both normal development and human disease. ..
- SIGNALING THROUGH RHO GTP/GDP EXCHANGE FACTORSPhilip B Wedegaertner; Fiscal Year: 2013..The research in this application will provide new knowledge about the functions of RhoGEFs and thus better define how the RhoGEFs can be therapeutically inhibited in disease without affecting their critical normal functions. ..
- Role of Lipoproteins in Chagasic heart diseaseJYOTHI FALGUNI NAGAJYOTHI; Fiscal Year: 2013..This is especially important as the epidemic of obesity, diabetes and dyslipidemia in Chagas endemic regions has the potential for significant interactions with this pathogen, thus altering th normal pathogenic process. ..
- Control of Renal Glutaminase Expression during AcidosisNorman P Curthoys; Fiscal Year: 2012..This knowledge will constitute the basis for biochemical and pharmacological studies that may improve the treatment of patients who present with chronic forms of metabolic acidosis. ..
- HORMONAL REGULATION OF MESSENGER RNA STABILITYDaniel R Schoenberg; Fiscal Year: 2013....
- Guo Zhang Zhu; Fiscal Year: 2014..Furthermore, collected information may offer insights into unexplained (or idiopathic) infertility in men and may lead to new strategies for male contraception and assisted reproduction. ..
- Jeffrey K Griffiths; Fiscal Year: 2016..We wish to see if it can be spread from, or to, the lungs, like influenza or tuberculosis. If it can spread in this way, then we can prevent people from getting infected by actions such as wearing masks and washing hands after coughing. ..
- Meiosis, SUMOylation and the ZIP3 Protein: Parallel Studies in Mouse and Yeast.Neil Hunter; Fiscal Year: 2012..Defects in these processes are linked to human infertility, miscarriage and genetic diseases, particularly cancer. A greater understanding of their mechanism and regulation will help us better understand the etiology of these diseases. ..
- Frank A Anania; Fiscal Year: 2016....
- Molecular Mechanism of cell-cell spread of Listeria in polarized epithelial cellsKEITH PATRICK IRETON; Fiscal Year: 2013..The goal of this research is to identify bacterial and human components that mediate Listeria spreading in an enterocyte cell line. These bacterial and host proteins may be suitable as targets for drugs that limit Listeria infection. ..
- GABA chaperoning of epilepsy-associated GABAA receptor mutants.Nancy J Leidenheimer; Fiscal Year: 2012..The proposed research investigates a possible mechanism for aiding the correct synthesis of these mutant receptors. Thus, these studies have relevance to the treatment of epilepsy. ..
- Regulation and Function of Urocortins and their ReceptorsAditi Bhargava; Fiscal Year: 2013..abstract_text> ..
- Autophagy in epidermal melanocyte: a protective or a destructive role?Vijayasaradhi Setaluri; Fiscal Year: 2013....
- Release of FGF1 and Pathology of AngiogenesisIgor Prudovsky; Fiscal Year: 2010..FGF1 is delivered to target cells through a specific stress-induced non-classical release pathway. The goal of this proposal is to understand the mechanism of FGF1 export and the role of this process in cardiovascular pathologies. ..
- Cellular Mechanisms of Lymphatic Muscle ContractilityMICHAEL JOHN DAVIS; Fiscal Year: 2012..These studies will investigate which proteins allow these vessels to contract so that specific therapeutic agents can be developed to correct lymphatic pump dysfunction and drainage of edematous tissues. ..
- Regulation of IL4 signal transduciton by poly (ADP-ribose) polymerase-1.Rahul Datta; Fiscal Year: 2011..Given the data demonstrating that PARP-1 inhibition abates of pulmonary inflammation in the mouse model of asthma, it is important to study the role of PARP-1 in inflammation in order to develop strategies in dealing with the disease. ..
- Analysis of the Spatiotemporal Regulation of LipolysisLisa M DiPilato; Fiscal Year: 2012..The information generated by this study will aid in the development of new therapeutics as well as our understanding of the mechanisms behind the spatiotemporal regulation of cellular signaling. ..
- IGF-II regulation of FABPs in the breast cancer survival disparity among AA womenTELEKA CASSANDRA CALDERON; Fiscal Year: 2012..Our studies will provide much needed information about the mechanisms involved in the protective effect lactation induces in the breast and will potentially offer new tools for preventive breast cancer treatment. ..
- Nemo-Like Kinase and the Pathogenesis of Spinal and Bulbar Muscular AtrophyTIFFANY WERLYNE TODD; Fiscal Year: 2013....
- Hongxin Dong; Fiscal Year: 2014..By advancing our understanding of the epigenetic mechanisms of drug efficacy, it will be possible to develop new psychotropic treatment strategies that maximize benefits while minimizing side effects. ..
- Role of complement in alpha-synuclein based models of Parkinson diseaseHEATHER ELIZABETH ALLEN; Fiscal Year: 2013..This proposal aims to provide evidence for a role for the complement system in PD pathology. This study would serve as rationale for the complement system as an innovative target for the development of PD therapies and biomarkers. ..
- Human Aging, Exercise &FMD: Translational PhysiologyDouglas R Seals; Fiscal Year: 2013.._OVIDED. University of Colorado-Boulder, Boulder, Colorado ..
- JESSICA OTIS; Fiscal Year: 2014....
- Alicia A McDonough; Fiscal Year: 2016....
- Irving H Zucker; Fiscal Year: 2016..Furthermore, w believe that these data will uncover new targets for therapy in CHF. ..
- Oxidative stress in regional cerebral blood flow alterations after cardiac arrestMioara D Manole; Fiscal Year: 2012....
- BONNIE ELYSSA GOULDROTHBERG; Fiscal Year: 2014..Thomas Lynch as Physician-in-Chief of the new cancer hospital. ..
- Lipoic Acid Therapy for Experimental Autoimmune EncephalomyelitisDENNIS NEIL BOURDETTE; Fiscal Year: 2013..Current FDA approved therapies for MS cost up to $35,000 a year and are only partially effective. Finding new, cost effective therapies that can slow the progression of MS is of obvious relevance to the health care mission of VHA. ..
- Regulation and Function of Germline RNP GranulesJENNIFER SCHISA; Fiscal Year: 2010..This research tests the hypothesis that large RNP granules play a protective role in maintaining oocyte viability and fertility and may be relevant to understanding the basis for infertility that occurs as women age. ..
- Soy isoflavones to augment radiotherapy of lung carcinomaGilda G Hillman; Fiscal Year: 2012..The results of this investigation will have high impact on the management of patients diagnosed with lung cancer. ..
- INTERACTIONS BETWEEN INTERMEDIATE FILAMENTS AND NUCLEUSRobert D Goldman; Fiscal Year: 2013..The role of lamins in the expression and elimination of abnormal chromosomes in breast cancer and prostate cancer cells will also be examined using our newly developed tools for lamin function. ..
- Function of the chromosomal kinase haspin in mitosisJONATHAN M HIGGINS; Fiscal Year: 2010..A greater knowledge of the role of human haspin in this process will help us understand the defects that underlie transformation and may lead to new approaches to block the division of cancer cells. ..
- TYPE IV SECRETION &SIGNAL TRANSDUCTION IN EHRLICHIOSISYasuko Rikihisa; Fiscal Year: 2013..abstract_text> ..
- Lali K Medina-Kauwe; Fiscal Year: 2016..As HER2+ breast cancer does not respond well to conventional therapies, this alternative therapy could provide a significant contribution to breast cancer treatment, and could be modified to target therapy to other types of cancer. ..
- MECHANISM OF LACRIMAL GLAND SECRETIONDarlene A Dartt; Fiscal Year: 2012....
- Using shared mechanisms of morphogenesis to study neural tube defectsJESSICA LEE SULLIVAN-BROWN; Fiscal Year: 2012..Therefore, I will study gene mutations that cause neural tube defects by using a tiny soil-dwelling worm, C. elegans, and the frog, Xenopus laevis, as simple systems to understand complex human diseases of the neural tube. ..
- Biology of desmosome-gap junctions in the testisDolores D Mruk; Fiscal Year: 2010..The study of desmosome-gap junctions in the testis will yield new and important insights into the biology of spermatogenesis. ..
- Role of Epithelial Chemokines in Kidney Injury-Molecule-1 Induced Renal FibrosisBenjamin D Humphreys; Fiscal Year: 2010..The proposed studies are therefore of great public health interest. ..
- Kaylen Lott; Fiscal Year: 2015..Overall, the proposed experiments will provide insight into the functions of an essential RBP in T. brucei and elucidate how arg methylation can modulate these functions. ..
- Denson G Fujikawa; Fiscal Year: 2016..Our finding that METH-induced subclinical RESDs in mice are associated with widespread neuronal damage could have important translational applicability. ..
- Acetaldehyde and Epithelial Junctions: Role of PP2A and Occludin PhosphorylationMITZI LEE DUNAGAN; Fiscal Year: 2011..Disruption of the barrier increases the risk of liver disease, pancreatitis, and cancer, so I am studying how acetaldehyde breaks connections between tight junction proteins and how to keep these connections intact. ..
- Endothelial Dysfunction in Older Adult Humans with the Metabolic SyndromeDEMETRA CHRISTOU; Fiscal Year: 2009....
- Klaus F Ley; Fiscal Year: 2016..PF4 or other molecules investigated here may emerge as targets for prevention or therapy of atherosclerosis and its complications. ..
- Modulation of Autoimmune Diabetes by N-acetyl-D-glucosamine Specific B cellsBRIAN L DIZON; Fiscal Year: 2010..Understanding the mechanisms involved in this link may provide insight on new strategies for earlier diagnosis, treatments, and prevention of T1D. ..
- Axial Flow Effects in Proximal TubuleTONG WANG WANG; Fiscal Year: 2012..Possible benefits include identification of target molecules, which may be blocked or modified in order to modulate sodium reabsorption by the kidney. ..
- CORNEAL LIPID METABOLISM AND RESPONSE TO INFLAMMATIONHaydee E P Bazan; Fiscal Year: 2010..If our hypothesis and its corollaries are correct, then PAF antagonists can become effective therapeutic tools for maintaining the transparency and integrity of the cornea. ..
- GENETIC ANALYSIS OF LUPUS AUTOIMMUNITYJudith James; Fiscal Year: 2000..This specific aim will depend upon a large genetic linkage study in human SLE currently being performed by Dr. Harley, and which already contains 30 pedigrees with a second cohort of an additional 30 pedigrees planned. ..
- NUCLEAR ACIDIC PROTEIN ANTIGENS IN RHEUMATIC DISEASESGORDON SHARP; Fiscal Year: 1990..The product of autoimmune response, the immune complex, will be biochemically characterized and its distribution studied for specific autoantibody-antigen reactivities...
- Human Zinc Transporter Zip 13 and Cellular Zinc HomeostasisJoel Walker; Fiscal Year: 2007..This proposal will begin to identify the genetic mechanisms controlling human Zip13 in response to low cellular zinc providing clues to how human cells regulate zinc metabolism. [unreadable] [unreadable] [unreadable]..
- BRAIN GLUTATHIONE S-TRANSFERASES AND NEUROTOXICITYJeffrey Johnson; Fiscal Year: 2000..The data generated from these studies will significantly increase our understanding of how GSTs are regulated in brain and further our knowledge of the role of these enzymes in modulating neurotoxicity. ..
- Development of a 3D cell culture model of the glomerular filtration barrierLESLIE BRUGGEMAN; Fiscal Year: 2009..These studies will allow us to understand not only how individual components of the filtration barrier work, but also how they interact with regard to both normal kidney function and how it is injured in disease. ..
- G2/M DNA DAMAGE CHECKPOINT IN BUDDING YEASTTed Weinert; Fiscal Year: 2000..He will then examine interactions with other mutants. A double mutant with a mec1 constitutive and a mutant in a gene that functions downstream of MEC1 should fail to show arrest at the restrictive temperature. ..
- NEUROIMMUNOLOGY AND PATHOLOGY OF NEUROCYSTICERCOSISJUDY TEALE; Fiscal Year: 2009..In vitro studies and inhibitors of MMPs will elucidate mechanisms. The studies outlined will provide a deeper understanding of the immunopathology of NCC and should provide important insights for developing therapeutic interventions. ..
- AUTOANTIBODIES TO CELLULAR MATRIX ANTIGENS IN CFSENG TAN; Fiscal Year: 2000..Overall, these studies are aimed at rigorously defining autoantibody reactivities in CFS and may have etiologic implications. ..
- THE SECRETORY IGA SYSTEM OF THE EYEDavid Sullivan; Fiscal Year: 1993....