plasmodium berghei

Summary

Summary: A protozoan parasite of rodents transmitted by the mosquito Anopheles dureni.

Top Publications

  1. pmc Metamorphosis of the malaria parasite in the liver is associated with organelle clearance
    Bamini Jayabalasingham
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Cell Res 20:1043-59. 2010
  2. pmc Mosquito immune defenses against Plasmodium infection
    Chris M Cirimotich
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe St, Baltimore, MD 21205, USA
    Dev Comp Immunol 34:387-95. 2010
  3. ncbi CD8+ T effector memory cells protect against liver-stage malaria
    Arturo Reyes-Sandoval
    The Jenner Institute, University of Oxford, Oxford OX3 7DQ, United Kingdom
    J Immunol 187:1347-57. 2011
  4. ncbi Heme oxygenase-1 and carbon monoxide suppress the pathogenesis of experimental cerebral malaria
    Ana Pamplona
    Instituto Gulbenkian de Ciencia, 2780 156 Oeiras, Portugal
    Nat Med 13:703-10. 2007
  5. pmc The systematic functional analysis of Plasmodium protein kinases identifies essential regulators of mosquito transmission
    Rita Tewari
    Institute of Genetics, QMC, University of Nottingham, Nottingham NG7 2UH, UK
    Cell Host Microbe 8:377-87. 2010
  6. pmc CD8+ T cells and IFN-γ mediate the time-dependent accumulation of infected red blood cells in deep organs during experimental cerebral malaria
    Carla Claser
    Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, Singapore, Singapore
    PLoS ONE 6:e18720. 2011
  7. pmc Leucine-rich repeat protein complex activates mosquito complement in defense against Plasmodium parasites
    Michael Povelones
    Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Science 324:258-61. 2009
  8. pmc Plasmepsin 4-deficient Plasmodium berghei are virulence attenuated and induce protective immunity against experimental malaria
    Roberta Spaccapelo
    Department of Experimental Medicine, University of Perugia, Via del Giochetto, 06122 Perugia, Italy
    Am J Pathol 176:205-17. 2010
  9. pmc Efficiency of salivary gland invasion by malaria sporozoites is controlled by rapid sporozoite destruction in the mosquito haemocoel
    JULIAN F HILLYER
    Center for Microbial and Plant Genomics and Department of Microbiology, University of Minnesota, 1500 Gortner Ave, Saint Paul, MN 55108, USA
    Int J Parasitol 37:673-81. 2007
  10. ncbi Transcription factor AP2-Sp and its target genes in malarial sporozoites
    Masao Yuda
    Department of Medical Zoology, Mie University School of Medicine, Mie, Tsu, 514 0001, Japan
    Mol Microbiol 75:854-63. 2010

Detail Information

Publications261 found, 100 shown here

  1. pmc Metamorphosis of the malaria parasite in the liver is associated with organelle clearance
    Bamini Jayabalasingham
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Cell Res 20:1043-59. 2010
    ..These observations lay the groundwork for further investigations on the developmental pathways implicated in the metamorphosis of the malaria parasite...
  2. pmc Mosquito immune defenses against Plasmodium infection
    Chris M Cirimotich
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe St, Baltimore, MD 21205, USA
    Dev Comp Immunol 34:387-95. 2010
    ....
  3. ncbi CD8+ T effector memory cells protect against liver-stage malaria
    Arturo Reyes-Sandoval
    The Jenner Institute, University of Oxford, Oxford OX3 7DQ, United Kingdom
    J Immunol 187:1347-57. 2011
    ..Thus, we identify persistent CD8 T(EM) populations as essential for vaccine-induced pre-erythrocytic protection against malaria, a finding that has important implications for vaccine design...
  4. ncbi Heme oxygenase-1 and carbon monoxide suppress the pathogenesis of experimental cerebral malaria
    Ana Pamplona
    Instituto Gulbenkian de Ciencia, 2780 156 Oeiras, Portugal
    Nat Med 13:703-10. 2007
    ..BALB/c mice infected with Plasmodium berghei ANKA upregulated HO-1 expression and activity and did not develop ECM...
  5. pmc The systematic functional analysis of Plasmodium protein kinases identifies essential regulators of mosquito transmission
    Rita Tewari
    Institute of Genetics, QMC, University of Nottingham, Nottingham NG7 2UH, UK
    Cell Host Microbe 8:377-87. 2010
    ..vector, we undertook a systematic functional analysis of ePKs in the genetically tractable rodent parasite Plasmodium berghei. Modeling domain signatures of conventional ePKs identified 66 putative Plasmodium ePKs...
  6. pmc CD8+ T cells and IFN-γ mediate the time-dependent accumulation of infected red blood cells in deep organs during experimental cerebral malaria
    Carla Claser
    Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, Singapore, Singapore
    PLoS ONE 6:e18720. 2011
    Infection with Plasmodium berghei ANKA (PbA) in susceptible mice induces a syndrome called experimental cerebral malaria (ECM) with severe pathologies occurring in various mouse organs...
  7. pmc Leucine-rich repeat protein complex activates mosquito complement in defense against Plasmodium parasites
    Michael Povelones
    Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Science 324:258-61. 2009
    ..promoting its cleavage or stabilization and its subsequent localization on the surface of midgut-invading Plasmodium berghei parasites, targeting them for destruction...
  8. pmc Plasmepsin 4-deficient Plasmodium berghei are virulence attenuated and induce protective immunity against experimental malaria
    Roberta Spaccapelo
    Department of Experimental Medicine, University of Perugia, Via del Giochetto, 06122 Perugia, Italy
    Am J Pathol 176:205-17. 2010
    ..decrease in the asexual blood-stage growth rate; however, PM4 deficiency in the rodent malaria parasite Plasmodium berghei results in significantly less virulence than that for the parental parasite. P...
  9. pmc Efficiency of salivary gland invasion by malaria sporozoites is controlled by rapid sporozoite destruction in the mosquito haemocoel
    JULIAN F HILLYER
    Center for Microbial and Plant Genomics and Department of Microbiology, University of Minnesota, 1500 Gortner Ave, Saint Paul, MN 55108, USA
    Int J Parasitol 37:673-81. 2007
    ..Only 2% of Anopheles gambiae haemocytes phagocytized Plasmodium berghei sporozoites, a rate insufficient to explain the extent of sporozoite clearance...
  10. ncbi Transcription factor AP2-Sp and its target genes in malarial sporozoites
    Masao Yuda
    Department of Medical Zoology, Mie University School of Medicine, Mie, Tsu, 514 0001, Japan
    Mol Microbiol 75:854-63. 2010
    ..These results indicate that AP2-Sp is a major transcription factor that regulates gene expression in the sporozoite stage...
  11. pmc Anopheles gambiae immune responses to human and rodent Plasmodium parasite species
    Yuemei Dong
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    PLoS Pathog 2:e52. 2006
    ..Furthermore, our data indicate that the mosquito is capable of sensing infected blood constituents in the absence of invading ookinetes, thereby inducing anti-Plasmodium immune responses...
  12. pmc A cyclic GMP signalling module that regulates gliding motility in a malaria parasite
    Robert W Moon
    Wellcome Trust Sanger Institute, Cambridge Hinxton, United Kingdom
    PLoS Pathog 5:e1000599. 2009
    ..These data demonstrate an important function for signalling through cGMP, and most likely PKG, in dynamically regulating ookinete gliding during the transmission of malaria to the mosquito...
  13. pmc Murine malaria parasite sequestration: CD36 is the major receptor, but cerebral pathology is unlinked to sequestration
    Blandine Franke-Fayard
    Department of Parasitology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 102:11468-73. 2005
    ..imaging of sequestration using transgenic luciferase-expressing parasites of the rodent malaria parasite Plasmodium berghei. These studies revealed that: (i) as expected, lung tissue is a major site, but, unexpectedly, adipose ..
  14. pmc Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development
    Gunnar R Mair
    Leiden Malaria Research Group, Department of Parasitology, Centre for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Pathog 6:e1000767. 2010
    ..purification coupled with mass-spectrometric analysis we identify a messenger ribonucleoprotein (mRNP) from Plasmodium berghei gametocytes defined by DOZI and the Sm-like factor CITH (homolog of worm CAR-I and fly Trailer Hitch)...
  15. pmc Dissecting the genetic basis of resistance to malaria parasites in Anopheles gambiae
    Stephanie A Blandin
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 326:147-50. 2009
    ..The successful application of rasRNAi in Anopheles suggests that it could also be applied to other organisms where RNAi is feasible to dissect complex phenotypes to the level of individual quantitative trait alleles...
  16. pmc Artemether and artesunate show the highest efficacies in rescuing mice with late-stage cerebral malaria and rapidly decrease leukocyte accumulation in the brain
    L Clemmer
    La Jolla Bioengineering Institute, 3535 General Atomics Court, Suite 210, San Diego, CA 92121, USA
    Antimicrob Agents Chemother 55:1383-90. 2011
    The murine model of cerebral malaria (ECM) caused by Plasmodium berghei ANKA (PbA) infection in susceptible mice has been extensively used for studies of pathogenesis and identification of potential targets for human CM therapeutics...
  17. pmc IP-10-mediated T cell homing promotes cerebral inflammation over splenic immunity to malaria infection
    Catherine Q Nie
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    PLoS Pathog 5:e1000369. 2009
    ..Thus, our results demonstrate that modulating homing of cellular immune responses to malaria is critical for reaching a balance between protective immunity and immunopathogenesis...
  18. pmc The murine cerebral malaria phenomenon
    Nicholas J White
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Trends Parasitol 26:11-5. 2010
    ..The value of the mouse model in identifying pathological processes or therapeutic interventions in human cerebral malaria is questionable...
  19. ncbi Coincident parasite and CD8 T cell sequestration is required for development of experimental cerebral malaria
    James A McQuillan
    Molecular Immunopathology Unit, The University of Sydney, Australia
    Int J Parasitol 41:155-63. 2011
    ..The results suggest that the development of the immunopathological response that causes CM depends on a continuous stimulus provided by parasitised red blood cells, either circulating or sequestered in small vessels...
  20. pmc The IFN-γ-inducible GTPase, Irga6, protects mice against Toxoplasma gondii but not against Plasmodium berghei and some other intracellular pathogens
    Oliver Liesenfeld
    Institute of Microbiology and Hygiene, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS ONE 6:e20568. 2011
    ..major, Listeria monocytogenes, Anaplasma phagocytophilum and Plasmodium berghei. Compared with wild-type mice, mice deficient in Irga6 showed increased susceptibility to oral and ..
  21. pmc IFN-γ-producing CD4+ T cells promote experimental cerebral malaria by modulating CD8+ T cell accumulation within the brain
    Ana Villegas-Mendez
    Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, London, UK
    J Immunol 189:968-79. 2012
    ..well established that IFN-γ is required for the development of experimental cerebral malaria (ECM) during Plasmodium berghei ANKA infection of C57BL/6 mice...
  22. pmc A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
    Benoit Malleret
    Laboratory of Malaria Immunobiology, Singapore Immunology Network SIgN, Agency for Science, Technology and Research A STAR, Biopolis, Singapore
    Sci Rep 1:118. 2011
    ..It also allows assessing the proportion of parasites at different developmental stages. Lastly, we demonstrate how this technique can be applied to antimalarial drug testing...
  23. ncbi Single-dose immunogenicity and protective efficacy of simian adenoviral vectors against Plasmodium berghei
    Arturo Reyes-Sandoval
    The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    Eur J Immunol 38:732-41. 2008
    ..C9), 83% (H5 and C7) and 67% (C6) of the mice, providing the first evidence of single-dose protection to Plasmodium berghei. Protection was afforded by the SAd despite high levels of pre-existing immunity to AdH5...
  24. ncbi High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
    Chris J Janse
    Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
    Mol Biochem Parasitol 145:60-70. 2006
    ..a new method of transfection, using non-viral Nucleofector technology, is described for the rodent parasite Plasmodium berghei. The transfection efficiency obtained (episomal and targeted integration into the genome) is in the range of ..
  25. pmc Reduced CD36-dependent tissue sequestration of Plasmodium-infected erythrocytes is detrimental to malaria parasite growth in vivo
    Jannik Fonager
    Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, 2333 ZA Leiden, Netherlands
    J Exp Med 209:93-107. 2012
    ..Irbc of the rodent parasite Plasmodium berghei ANKA sequester in a fashion analogous to P. falciparum by adhering to the host receptor CD36...
  26. pmc The Plasmodium eukaryotic initiation factor-2alpha kinase IK2 controls the latency of sporozoites in the mosquito salivary glands
    Min Zhang
    Michael Heidelberger Division, Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Exp Med 207:1465-74. 2010
    ..Thus, to complete their life cycle, Plasmodium sporozoites exploit the mechanism that regulates stress responses in eukaryotic cells...
  27. pmc Progression of Plasmodium berghei through Anopheles stephensi is density-dependent
    Robert E Sinden
    Division of Cell and Molecular Biology, Faculty of Life Sciences, Imperial College London, London, United Kingdom
    PLoS Pathog 3:e195. 2007
    ..Here we establish infections with defined numbers of the rodent malaria parasite Plasmodium berghei to examine how parasite density at each stage of development (gametocytes; ookinetes; oocysts and ..
  28. pmc Accumulation of Plasmodium berghei-infected red blood cells in the brain is crucial for the development of cerebral malaria in mice
    Fernanda G Baptista
    Instituto Gulbenkian de Ciencia, Oeiras, Portugal
    Infect Immun 78:4033-9. 2010
    ..It was shown that Plasmodium berghei ANKA-induced cerebral malaria was prevented in 100% of mice depleted of CD8+ T cells 1 day prior to the ..
  29. pmc Anopheles fibrinogen-related proteins provide expanded pattern recognition capacity against bacteria and malaria parasites
    Yuemei Dong
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 284:9835-44. 2009
    ..Our findings indicate that the A. gambiae FREP gene family plays a central role in the mosquito innate immune system and provides an expanded pattern recognition and anti-microbial defense repertoire...
  30. pmc Nitric oxide protection against murine cerebral malaria is associated with improved cerebral microcirculatory physiology
    Pedro Cabrales
    La Jolla Bioengineering Institute, La Jolla, CA 92037, USA
    J Infect Dis 203:1454-63. 2011
    Cerebral malaria (CM) is a leading cause of death in Plasmodium falciparum infections. In the Plasmodium berghei ANKA (PbA) murine model, CM pathogenesis is associated with low nitric oxide (NO) bioavailability and brain microcirculatory ..
  31. ncbi Immune-mediated mechanisms of parasite tissue sequestration during experimental cerebral malaria
    Fiona H Amante
    Australian Centre for Vaccine Development, Brisbane, Queensland, Australia
    J Immunol 185:3632-42. 2010
    ..These findings provide novel insights into immune-mediated mechanisms of ECM pathogenesis and highlight the potential of T cells to both prevent and promote infectious diseases...
  32. pmc Protective CD8 T cells against Plasmodium liver stages: immunobiology of an 'unnatural' immune response
    Michael Glen Overstreet
    Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Immunol Rev 225:272-83. 2008
    ..Finally, we discuss the impact of the immunodominant CS protein on this protection and the implications of these findings for vaccine design...
  33. pmc Plasmodium berghei ANKA infection increases Foxp3, IL-10 and IL-2 in CXCL-10 deficient C57BL/6 mice
    Bismark Y Sarfo
    Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, 720 Westview Drive, South West, Atlanta Georgia, GA 30310, USA
    Malar J 10:69. 2011
    ..Interleukin-2 (IL-2), a pro-inflammatory cytokine implicated in malaria pathogenesis has also been shown to be a key regulator of Foxp3. However the role of Foxp3 in resistant murine CM is not well understood...
  34. ncbi Protection from Plasmodium berghei infection by priming and boosting T cells to a single class I-restricted epitope with recombinant carriers suitable for human use
    M Plebanski
    Institute of Molecular Medicine, Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, GB
    Eur J Immunol 28:4345-55. 1998
    ..Immunization with recombinant Ty and MVA containing a single Plasmodium berghei class I-binding epitope provided 95% sterile protection against malaria in mice...
  35. pmc Visualisation and quantitative analysis of the rodent malaria liver stage by real time imaging
    Ivo H J Ploemen
    Department of Medical Microbiology, Radboud University Nijmegen Medical Centre RUNMC, Nijmegen, The Netherlands
    PLoS ONE 4:e7881. 2009
    ....
  36. ncbi Erythrocyte remodeling in Plasmodium berghei infection: the contribution of SEP family members
    Chiara Currà
    Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Rome, Italy
    Traffic 13:388-99. 2012
    ..We show here that members of the rodent Plasmodium berghei family are components of the parasitophorous vacuole membrane (PVM), which surrounds the parasite throughout ..
  37. pmc Algorithms to predict cerebral malaria in murine models using the SHIRPA protocol
    Yuri C Martins
    Laboratório de Pesquisas em Malária, Instituto Oswaldo Cruz, FIOCRUZ, Brasil, 4365 Manguinhos, Cep 21045 900 Rio de Janeiro RJ, Brasil
    Malar J 9:85. 2010
    b>Plasmodium berghei ANKA infection in C57Bl/6 mice induces cerebral malaria (CM), which reproduces, to a large extent, the pathological features of human CM...
  38. pmc Wolbachia strain wAlbB enhances infection by the rodent malaria parasite Plasmodium berghei in Anopheles gambiae mosquitoes
    Grant L Hughes
    Department of Entomology, The Pennsylvania State University, University Park, PA, USA
    Appl Environ Microbiol 78:1491-5. 2012
    ..and wMelPop from Drosophila melanogaster, on the vector competence of Anopheles gambiae challenged with Plasmodium berghei. We show that the wAlbB strain significantly increases P...
  39. ncbi Perforin-dependent brain-infiltrating cytotoxic CD8+ T lymphocytes mediate experimental cerebral malaria pathogenesis
    Josianne Nitcheu
    Institut National de la Sante et de la Recherche Medicale, Unité 511, Immunobiologie Cellulaire et Moleculaire des Infections Parasitaires, CHU Pitie Salpetriere, Universite Pierre et Marie Curie, 91 Boulevard de l Hopital, 75634 Paris Cedex 13, France
    J Immunol 170:2221-8. 2003
    Experimental cerebral malaria (ECM) resulting from Plasmodium berghei ANKA infection involves T lymphocytes. However, the mechanisms of T cell-mediated pathogenesis remain unknown...
  40. pmc Heparan sulfate proteoglycans provide a signal to Plasmodium sporozoites to stop migrating and productively invade host cells
    Alida Coppi
    Department of Medical Parasitology, 341 East 25th Street, New York University School of Medicine, New York, NY 10010, USA
    Cell Host Microbe 2:316-27. 2007
    ..Here, we show that sporozoites of the rodent malaria parasite Plasmodium berghei use the sulfation level of host heparan sulfate proteoglycans (HSPGs) to navigate within the mammalian host...
  41. pmc Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria
    Gabriele S V Campanella
    Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 105:4814-9. 2008
    ..IP-10 and Mig (CXCL9) were highly induced in the brains of mice with murine cerebral malaria caused by Plasmodium berghei ANKA...
  42. ncbi Pathogenic T cells in cerebral malaria
    Laurent Renia
    Department of Immunology, Institut Cochin, INSERM U567, CNRS UMR 8104, Universite Rene Descartes, Hopital Cochin, Paris, France
    Int J Parasitol 36:547-54. 2006
    ..However, this phenomenon cannot explain all aspects of the pathogenesis of CM. The use of an animal model, Plasmodium berghei ANKA in mice, has allowed the identification of specific pathological components of CM...
  43. ncbi Simple and sensitive antimalarial drug screening in vitro and in vivo using transgenic luciferase expressing Plasmodium berghei parasites
    B Franke-Fayard
    Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, Leiden, The Netherlands
    Int J Parasitol 38:1651-62. 2008
    ..The standard methodology of anti-malarial drug screening and validation, which includes testing in rodent models of malaria, can be improved by the incorporation of such assays...
  44. ncbi Involvement of IFN-gamma receptor-medicated signaling in pathology and anti-malarial immunity induced by Plasmodium berghei infection
    V Amani
    INSERM U511, CHU Pitie Salpetriere, Universite Paris, France
    Eur J Immunol 30:1646-55. 2000
    ..KO mice infected with 106 parasitized erythrocytes (PE) of Plasmodium berghei ANKA (PbA) did not develop ECM, but they had high parasitemia and died earlier than WT mice which did not ..
  45. pmc The role of metacaspase 1 in Plasmodium berghei development and apoptosis
    Ludovic Le Chat
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Mol Biochem Parasitol 153:41-7. 2007
    ..We have generated Plasmodium berghei parasites in which the PbMC1coding sequence is removed and replaced with a green fluorescent reporter gene ..
  46. pmc RATT: Rapid Annotation Transfer Tool
    Thomas D Otto
    Parasite Genomics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, CB10 1SA, UK
    Nucleic Acids Res 39:e57. 2011
    ..5 Mb chromosome from a malaria parasite can be annotated in less than five minutes with only modest computational resources. RATT is available at http://ratt.sourceforge.net...
  47. ncbi NK cells stimulate recruitment of CXCR3+ T cells to the brain during Plasmodium berghei-mediated cerebral malaria
    Diana S Hansen
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Immunol 178:5779-88. 2007
    ..been shown that the NK complex is a genetic determinant of murine cerebral malaria pathogenesis mediated by Plasmodium berghei ANKA...
  48. pmc The Plasmodium serine-type SERA proteases display distinct expression patterns and non-essential in vivo roles during life cycle progression of the malaria parasite
    Elyzana D Putrianti
    Max Planck Institute for Infection Biology, Berlin, Germany
    Cell Microbiol 12:725-39. 2010
    ....
  49. pmc Kinome-wide RNAi screen implicates at least 5 host hepatocyte kinases in Plasmodium sporozoite infection
    Miguel Prudêncio
    Unidade de Malaria, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal
    PLoS Pathog 4:e1000201. 2008
    ..This study represents the first comprehensive, functional genomics-driven identification of novel host factors involved in Plasmodium sporozoite infection...
  50. ncbi Control of pathogenic CD8+ T cell migration to the brain by IFN-gamma during experimental cerebral malaria
    E Belnoue
    Department of Immunology, Institute Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Parasite Immunol 30:544-53. 2008
    ..studies have shown that IFN-gamma is essential for the pathogenesis of cerebral malaria (CM) induced by Plasmodium berghei ANKA (PbA) in mice...
  51. pmc Severe malarial anemia of low parasite burden in rodent models results from accelerated clearance of uninfected erythrocytes
    Krystal J Evans
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    Blood 107:1192-9. 2006
    ..Anemia was significantly alleviated by depletion of either phagocytic cells or CD4+ T lymphocytes. This study demonstrates that immunologic mechanisms may contribute to SMA by promoting the accelerated turnover of uninfected RBCs...
  52. pmc Paternal effect of the nuclear formin-like protein MISFIT on Plasmodium development in the mosquito vector
    Ellen S C Bushell
    Department of Life Sciences, Imperial College London, London, UK
    PLoS Pathog 5:e1000539. 2009
    ..of MISFIT, the first protein with paternal effect on the development of the rodent malaria parasite Plasmodium berghei in Anopheles mosquitoes...
  53. ncbi Malaria-specific and nonspecific activation of CD8+ T cells during blood stage of Plasmodium berghei infection
    Mana Miyakoda
    Department of Molecular Microbiology and Immunology, Division of Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
    J Immunol 181:1420-8. 2008
    ..Studies using a rodent model of Plasmodium berghei ANKA infection established that CD8(+) T cells are involved in the pathogenesis of cerebral malaria...
  54. ncbi High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei
    Chris J Janse
    Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
    Nat Protoc 1:346-56. 2006
    This protocol describes a method of genetic transformation for the rodent malaria parasite Plasmodium berghei with a high transfection efficiency of 10(-3)-10(-4)...
  55. pmc CD4+ CD25+ regulatory T cells suppress CD4+ T-cell function and inhibit the development of Plasmodium berghei-specific TH1 responses involved in cerebral malaria pathogenesis
    Catherine Q Nie
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Infect Immun 75:2275-82. 2007
    The infection of mice with Plasmodium berghei ANKA constitutes the best available mouse model for human Plasmodium falciparum-mediated cerebral malaria, a devastating neurological syndrome that kills nearly 2.5 million people every year...
  56. pmc Development and application of a positive-negative selectable marker system for use in reverse genetics in Plasmodium
    Joanna A M Braks
    Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, The Netherlands
    Nucleic Acids Res 34:e39. 2006
    ..This is exacerbated for the rodent parasite Plasmodium berghei as selection of mutants is performed in vivo in laboratory rodents...
  57. ncbi An atypical mitogen-activated protein kinase controls cytokinesis and flagellar motility during male gamete formation in a malaria parasite
    Rita Tewari
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Mol Microbiol 58:1253-63. 2005
    ..an essential function for an atypical mitogen-activated protein kinase of the rodent malaria parasite Plasmodium berghei, Pbmap-2, in male sexual differentiation and parasite transmission to the mosquito...
  58. ncbi Independent roles of apical membrane antigen 1 and rhoptry neck proteins during host cell invasion by apicomplexa
    Donatella Giovannini
    Institut Pasteur, Unité de Biologie et Génétique du Paludisme, 75724 Paris, France
    Cell Host Microbe 10:591-602. 2011
    ..Here we find that, in Plasmodium berghei, while both AMA1 and RON4 are important for merozoite invasion of erythrocytes, only RON4 is required for ..
  59. ncbi The Kennedy phospholipid biosynthesis pathways are refractory to genetic disruption in Plasmodium berghei and therefore appear essential in blood stages
    Sandrine Dechamps
    Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235, CNRS Universite Montpellier 2, Place Eugene Bataillon, cc107, Montpellier 34095, Cedex 05, France
    Mol Biochem Parasitol 173:69-80. 2010
    ..Here, we have studied the essential nature of the Kennedy pathways in Plasmodium berghei, a rodent malaria parasite. Sequence analysis of the P...
  60. ncbi Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice
    Blandine Franke-Fayard
    Malaria Research Group, Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
    Nat Protoc 1:476-85. 2006
    ..a methodology for imaging the sequestration of infected erythrocytes of the rodent malaria parasite Plasmodium berghei in the bodies of live mice or in dissected organs, using a transgenic parasite that expresses luciferase...
  61. ncbi Cell- rather than antibody-mediated immunity leads to the development of profound thrombocytopenia during experimental Plasmodium berghei malaria
    Irene Gramaglia
    La Jolla Bioengineering Institute, La Jolla, CA 92037, USA
    J Immunol 175:7699-707. 2005
    ..However, we report that Abs account for at most 15% of platelet destruction as Plasmodium berghei-infected B cell-deficient mice exhibited profound thrombocytopenia (83%) as did C57BL/6 controls (98%)...
  62. ncbi Plasmodium berghei-infected primary hepatocytes process and present the circumsporozoite protein to specific CD8+ T cells in vitro
    Silayuv E Bongfen
    Department of Biochemistry, University of Lausanne, Epalinges, Switzerland
    J Immunol 178:7054-63. 2007
    ..We investigated how primary hepatocytes from BALB/c mice process the CSP of Plasmodium berghei after live sporozoite infection and present CSP-derived peptides to specific H-2K(d)-restricted CD8(+) T ..
  63. ncbi Antimalarial activity of Bidens pilosa L. (Asteraceae) ethanol extracts from wild plants collected in various localities or plants cultivated in humus soil
    Valter F Andrade-Neto
    Laboratory of Malaria, Centro de Pesquisas Rene Rachou Fiocruz, Av Augusto de Lima 1715, Belo Horizonte, Minas Gerais CEP 30190 002, Brazil
    Phytother Res 18:634-9. 2004
    ....
  64. ncbi A critical role of Fc receptor-mediated antibody-dependent phagocytosis in the host resistance to blood-stage Plasmodium berghei XAT infection
    T Yoneto
    Department of Allergology and Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    J Immunol 166:6236-41. 2001
    b>Plasmodium berghei XAT is an irradiation-induced attenuated variant derived from the lethal strain P. berghei NK65, and its blood-stage parasites are spontaneously cleared in immune competent mice...
  65. pmc The generation and evaluation of recombinant human IgA specific for Plasmodium falciparum merozoite surface protein 1-19 (PfMSP1 19)
    Jianguo Shi
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, NG7 2UH, UK
    BMC Biotechnol 11:77. 2011
    ..Although human serum immunoglobulin A (IgA) is the second most abundant class of antibody in the circulation, its contribution, if any, to protective responses against malaria is not clear...
  66. pmc CNS hypoxia is more pronounced in murine cerebral than noncerebral malaria and is reversed by erythropoietin
    Casper Hempel
    Centre for Medical Parasitology, Department of Clinical Microbiology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
    Am J Pathol 179:1939-50. 2011
    ..To assess the role of cerebral hypoxia, we used two experimental models of CM, Plasmodium berghei ANKA in CBA and C57BL/6 mice, and two models of malaria without neurologic signs, P...
  67. pmc Energy metabolism affects susceptibility of Anopheles gambiae mosquitoes to Plasmodium infection
    Jose Henrique M Oliveira
    Laboratório de Bioquímica de Artrópodes Hematófagos, Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
    Insect Biochem Mol Biol 41:349-55. 2011
    ..and the mRNA levels of lactate dehydrogenase increased in the midgut, while the prevalence and intensity of Plasmodium berghei infection were significantly reduced...
  68. pmc Protein kinase C θ deficiency increases resistance of C57BL/6J mice to Plasmodium berghei infection-induced cerebral malaria
    Ariel Ohayon
    The Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
    Infect Immun 78:4195-205. 2010
    ..We tested the consequences of PKCθ ablation on the host response to infection by Plasmodium berghei ANKA (PbA). We found that both PKCθ(+/+) and PKCθ(-/-) C57BL/6J mice are susceptible to infection with PbA...
  69. ncbi Identification of a novel cerebral malaria susceptibility locus (Berr5) on mouse chromosome 19
    J Berghout
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Genes Immun 11:310-8. 2010
    ..component to CM can be investigated in mouse models that vary in degree of susceptibility to infection with Plasmodium berghei ANKA...
  70. pmc Experimentally controlled downregulation of the histone chaperone FACT in Plasmodium berghei reveals that it is critical to male gamete fertility
    Eliane C Laurentino
    Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
    Cell Microbiol 13:1956-74. 2011
    ..We identified a Plasmodium berghei protein, termed FACT-L, with homology to the SPT16 subunit of FACT...
  71. ncbi PbGEST mediates malaria transmission to both mosquito and vertebrate host
    Arthur M Talman
    Department of Life Sciences, Imperial College London, SW72AZ London, UK
    Mol Microbiol 82:462-74. 2011
    ..We identified a Plasmodium berghei secreted protein (PBANKA_131270) that plays distinct roles in both the mammal-to-mosquito and the mosquito-..
  72. ncbi Blood-stage Plasmodium berghei infection generates a potent, specific CD8+ T-cell response despite residence largely in cells lacking MHC I processing machinery
    Lei Shong Lau
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    J Infect Dis 204:1989-96. 2011
    Murine cerebral malaria is a complex disease caused by Plasmodium berghei ANKA infection. Several cell types, including CD8(+) T cells, are essential effectors of disease...
  73. pmc Inhaled nitric oxide reduces endothelial activation and parasite accumulation in the brain, and enhances survival in experimental cerebral malaria
    Lena Serghides
    SA Rotman Laboratories, McLaughlin Rotman Centre for Global Health, Tropical Disease Unit, Division of Infectious Diseases, Department of Medicine, University Health Network Toronto General Hospital, and University of Toronto, Toronto, Canada
    PLoS ONE 6:e27714. 2011
    ..100% survival in the artesunate plus iNO group, p = 0.03). These data support the clinical investigation of inhaled nitric oxide as a novel adjunctive therapy in patients with severe malaria...
  74. pmc Efficacy of different nitric oxide-based strategies in preventing experimental cerebral malaria by Plasmodium berghei ANKA
    Yuri C Martins
    Center for Malaria Research, La Jolla Bioengineering Institute, San Diego, California, United States of America
    PLoS ONE 7:e32048. 2012
    ..plays a role in the pathogenesis of human as well as of experimental cerebral malaria (ECM) caused by Plasmodium berghei ANKA (PbA)...
  75. pmc Fighting malaria with engineered symbiotic bacteria from vector mosquitoes
    Sibao Wang
    Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 109:12734-9. 2012
    ..inhibited development of the human malaria parasite Plasmodium falciparum and rodent malaria parasite Plasmodium berghei by up to 98%...
  76. pmc A Plasmodium calcium-dependent protein kinase controls zygote development and transmission by translationally activating repressed mRNAs
    Sarah Sebastian
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
    Cell Host Microbe 12:9-19. 2012
    ..Using the rodent malaria parasite Plasmodium berghei, we show that CDPK1, which is known to be essential in the asexual blood stage of the parasite, is expressed ..
  77. pmc Beta interferon suppresses the development of experimental cerebral malaria
    Craig N Morrell
    Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Infect Immun 79:1750-8. 2011
    ..b>Plasmodium berghei-infected mice treated with IFN-β died later and showed increased survival, with improved blood-brain ..
  78. ncbi The role of cerebral oedema in the pathogenesis of cerebral malaria
    L A Sanni
    Division of Parasitology, National Institute for Medical Research, London, UK
    Redox Rep 6:137-42. 2001
    ....
  79. pmc Evidence that mutant PfCRT facilitates the transmission to mosquitoes of chloroquine-treated Plasmodium gametocytes
    Andrea Ecker
    Department of Microbiology and Immunology, Columbia University Medical Center, New York, New York 10032, USA
    J Infect Dis 203:228-36. 2011
    ..drug pressure, we have introduced a mutant or wild-type pfcrt allele into the rodent model malarial parasite Plasmodium berghei. Our results show that mutant PfCRT from the chloroquine-resistant 7G8 strain has no effect on asexual blood ..
  80. pmc VEGF promotes malaria-associated acute lung injury in mice
    Sabrina Epiphanio
    Unidade de Malaria, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal
    PLoS Pathog 6:e1000916. 2010
    ....
  81. pmc Multiple roles for Plasmodium berghei phosphoinositide-specific phospholipase C in regulating gametocyte activation and differentiation
    Andreas C Raabe
    UMR5235, CNRS Universite Montpellier 2, Place Eugene Bataillon, Montpellier Cedex 5, France
    Cell Microbiol 13:955-66. 2011
    ..The synchronized development of Plasmodium berghei gametocytes relies on rapid calcium release from internal stores within 10 s of gametocytes being exposed ..
  82. ncbi Plasmodium berghei: parasite clearance after treatment with dihydroartemisinin in an asplenic murine malaria model
    Brioni R Moore
    School of Pharmacy, Curtin University of Technology, P O Box U1987, Perth, WA 6845, Australia
    Exp Parasitol 118:458-67. 2008
    ..Mice were inoculated with Plasmodium berghei parasitised erythrocytes and received a single dose of dihydroartemisinin 56 h later, at 2-5% parasitaemia...
  83. pmc Imaging of Plasmodium liver stages to drive next-generation antimalarial drug discovery
    Stephan Meister
    Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 334:1372-7. 2011
    ..The open-source chemical tools resulting from our effort provide starting points for future drug discovery programs, as well as opportunities for researchers to investigate the biology of exo-erythrocytic forms...
  84. ncbi Involvement of actin and myosins in Plasmodium berghei ookinete motility
    Inga Siden-Kiamos
    Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Vassilika Vouton, P O Box 1385, 71110 Heraklion, Crete, Greece
    Mol Biochem Parasitol 150:308-17. 2006
    ..Following treatment with jasplakinolide, the apparent level of detectable actin appears to substantially increase and becomes concentrated in a discrete area in the basal pole of the ookinete...
  85. ncbi Localisation and timing of expression of putative Plasmodium berghei rhoptry proteins in merozoites and sporozoites
    Marta Tufet-Bayona
    The Malaria Centre, and the Division of Cell and Molecular Biology, Imperial College London, London, UK
    Mol Biochem Parasitol 166:22-31. 2009
    ....
  86. pmc Novel surface display system for proteins on non-genetically modified gram-positive bacteria
    Tjibbe Bosma
    BiOMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Appl Environ Microbiol 72:880-9. 2006
    ..The versatility and flexibility of the display and delivery technology were demonstrated by investigating enzyme immobilization and nasal vaccine applications...
  87. pmc Blood-stage Plasmodium infection induces CD8+ T lymphocytes to parasite-expressed antigens, largely regulated by CD8alpha+ dendritic cells
    Rachel J Lundie
    Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Proc Natl Acad Sci U S A 105:14509-14. 2008
    ..Finally, using three different experimental approaches, we provide evidence that CTL specific for parasite-expressed antigens contribute to ECM...
  88. ncbi Magnetic resonance spectroscopy reveals an impaired brain metabolic profile in mice resistant to cerebral malaria infected with Plasmodium berghei ANKA
    Marie France Penet
    Centre de Resonance Magnetique Biologique et Medicale, Unité Mixte de Recherche UMR CNRS 6612, Facultéde Médecine la Timone, UniversitédelaMéditerranée, Marseille, France
    J Biol Chem 282:14505-14. 2007
    ..a combined in vivo/in vitro metabolic-based approach, we investigated the putative pathogenic effects of Plasmodium berghei ANKA on brain, in a mouse strain developing malaria but resistant to cerebral malaria...
  89. pmc A mitogen-activated protein kinase regulates male gametogenesis and transmission of the malaria parasite Plasmodium berghei
    Radha Rangarajan
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115, USA
    EMBO Rep 6:464-9. 2005
    ..Here, we show that a Plasmodium berghei homologue of Plasmodium falciparum mitogen-activated kinase-2 (Pfmap-2), a gametocyte-specific mitogen-..
  90. ncbi Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites
    Stefanie Graewe
    Department of Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Biotechnol J 4:895-902. 2009
    ..We observed that a red fluorescent transgenic malaria parasite of rodents, Plasmodium berghei-RedStar, is suitable for in vitro live imaging experiments but bleaches rapidly upon illumination in ..
  91. pmc Three members of the 6-cys protein family of Plasmodium play a role in gamete fertility
    Melissa R van Dijk
    Laboratory for Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
    PLoS Pathog 6:e1000853. 2010
    ....
  92. pmc ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology
    Valery Combes
    Laboratory of Immunopathology, Faculty of Medicine, IFR48, Universite de la Mediterranee, 27, Bd Jean Moulin, F 13385 Marseille cedex 05, France
    Am J Pathol 166:295-302. 2005
    ..On analysis of histological and systemic parameters we evidenced an impairment of cellular responses to Plasmodium berghei ANKA infection in ABCA1(-/-) mice, as shown by lower plasma tumor necrosis factor levels, a weaker up-..
  93. ncbi Egress of Plasmodium berghei gametes from their host erythrocyte is mediated by the MDV-1/PEG3 protein
    Marta Ponzi
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Rome, Italy
    Cell Microbiol 11:1272-88. 2009
    ..We here report the characterization of a Plasmodium berghei protein that is involved in egress of both male and female gametes from the host erythrocyte...
  94. pmc Hemocyte differentiation mediates innate immune memory in Anopheles gambiae mosquitoes
    Janneth Rodrigues
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20892, USA
    Science 329:1353-5. 2010
    ..In mosquitoes, differentiation of hemocytes was necessary and sufficient to confer innate immune memory...
  95. pmc Spiroindolones, a potent compound class for the treatment of malaria
    Matthias Rottmann
    Swiss Tropical and Public Health Institute, Parasite Chemotherapy, CH 4002 Basel, Switzerland
    Science 329:1175-80. 2010
    ..The optimized spiroindolone NITD609 shows pharmacokinetic properties compatible with once-daily oral dosing and has single-dose efficacy in a rodent malaria model...
  96. pmc A sporozoite asparagine-rich protein controls initiation of Plasmodium liver stage development
    Olivier Silvie
    Department of Parasitology, Heidelberg University School of Medicine, Heidelberg, Germany
    PLoS Pathog 4:e1000086. 2008
    ..Gene disruption in Plasmodium berghei results in complete loss of sporozoite infectivity to rodents, due to early developmental arrest after ..
  97. ncbi Erythrocyte G protein-coupled receptor signaling in malarial infection
    Travis Harrison
    Department of Pathology, Feinberg School of Medicine, Northwestern University, 303 Chicago Avenue, Chicago, IL 60611, USA
    Science 301:1734-6. 2003
    ..berghei infections in an in vivo mouse model. Thus, signaling via the erythrocyte beta2-adrenergic receptor and Galphas may regulate malarial infection across parasite species...
  98. pmc Superior antimalarial immunity after vaccination with late liver stage-arresting genetically attenuated parasites
    Noah S Butler
    Department of Microbiology, University of Iowa, 3 512 Bowen Science Building, Iowa City, IA 52242, USA
    Cell Host Microbe 9:451-62. 2011
    ....
  99. ncbi Antimalarial activity of phenazines from lapachol, beta-lapachone and its derivatives against Plasmodium falciparum in vitro and Plasmodium berghei in vivo
    Valter F de Andrade-Neto
    Centro de Pesquisas Rene Rachou, Fiocruz and Departamento de Parasitologia Universidade Federal de Minas Gerais UFMG, Belo Horizonte, MG, Brazil
    Bioorg Med Chem Lett 14:1145-9. 2004
    ..67 to 9.44 microM. The two most active ones were also tested in vivo against Plasmodium berghei in mice, in parallel with lapachol and beta-lapachone...
  100. ncbi Cytokine and chemokine mRNA expression in neutrophils from CBA/NSlc mice infected with Plasmodium berghei ANKA that induces experimental cerebral malaria
    L Chen
    Department of Biomedical Science, College of Medicine at Rockford, University of Illinois, 1601 Parkview Avenue, Rockford, IL 61107, USA
    Parasitol Int 50:139-43. 2001
    ..Therefore, this study confirms our hypothesis that neutrophils may play a role in the pathogenesis of ECM via their expression of cytokines or chemokines...
  101. pmc A constitutive pan-hexose permease for the Plasmodium life cycle and transgenic models for screening of antimalarial sugar analogs
    Martin Blume
    Department of Molecular Parasitology, Humboldt University, Berlin, Germany
    FASEB J 25:1218-29. 2011
    ..Here, we reveal that hexose transporter 1 (HT1) of Plasmodium berghei can transport glucose (K(m)~87 μM), mannose (K(i)~93 μM), fructose (K(i)~0...

Research Grants40

  1. LIVER MEMORY CD8 T CELLS IN PROTECTION TO MALARIA
    Urszula Krzych; Fiscal Year: 2009
    ..The mouse model of sterile and lasting protection induced by radiation-attenuated Plasmodium berghei sporozoites is an excellent system for addressing the issues surrounding memory CDS T cells...
  2. Nitric Oxide Restoration &Heme Detoxification as Adjunctive Therapies for Cerebr
    LEONARDO JOSE DE MOURA CARVALHO; Fiscal Year: 2012
    ..In the murine model of CM by Plasmodium berghei ANKA (PbA), CM has been associated to low nitric oxide (NO) bioavailability, and treatment with exogenous NO ..
  3. Cerebral Malaria: Mechanisms of disease and neurological salvage
    Mahalia S Desruisseaux; Fiscal Year: 2013
    ..In the mouse model of cerebral malaria, mice are infected with the ANKA strain of Plasmodium berghei and compared to a non cerebral malaria model- mice infected with the NK65 strain of P. berghei...
  4. The role of the terminal complement pathway in experimental cerebral malaria.
    Theresa N Ramos; Fiscal Year: 2012
    ..the complement system in the development and pathogenesis of experimental cerebral malaria (ECM) using the Plasmodium berghei ANKA (PbA) model system [3, 5, 6]...
  5. Mechanisms of effect of iron status &interventions on malaria &other infections
    Steven L Spitalnik; Fiscal Year: 2012
    ..For these studies, we selected rodent malarial species, Plasmodium berghei and P...
  6. Efficacy evaluation of traditionally used antimalarial plants
    ROCKY T GRAZIOSE; Fiscal Year: 2012
    ..These substances will be tested in animal model of malaria using C57Bl mice infected with the Plasmodium berghei strain. This model will also allow synergistic and pharmacodynamic investigations of promising extracts...
  7. Effects of Blood Meal on Cellular Immunity in Mosquitoes
    William B Bryant; Fiscal Year: 2013
    ..To determine what role hemocyte homeostasis has on immunity, Plasmodium berghei infection intensities will be determined in reverse genetic backgrounds of altered signaling in hemocytes ..
  8. Investigating the Role of Malaria Rhoptry-Associated Protein 2 in Severe Anemia
    NOE DAVID GOMEZ; Fiscal Year: 2010
    ..to determine the in vivo role of RAP2, our studies will be conducted using the malaria model rodent parasite Plasmodium berghei. The specific aims have been designed to characterize the P. berghei orthologue of the P...
  9. Investigating the molecular basis of primaquine resistance
    Elizabeth A Winzeler; Fiscal Year: 2011
    ..I will develop methods for identifying single base changes in evolved drug resistant lines of Plasmodium berghei, a rodent model of human malaria...
  10. Chemical Methods for Ferrous Iron Dependent Drug Delivery
    ADAM ROBERT RENSLO; Fiscal Year: 2013
    ..systems and demonstrated the iron(II)-dependent and parasite-selective delivery of a cysteine inhibitor to Plasmodium berghei parasites in infected mice...
  11. Plasmodium Sporozoites Motility and Cell Invasion
    Dyann Wirth; Fiscal Year: 2006
    ..cell biological and genetic approaches to elucidate the functional role of TRAP in host cell invasion by Plasmodium berghei. Our working hypothesis is that TRAP acts as a molecular link between the parasite cortical microfilaments ..
  12. TRAP AS A CANDIDATE MALARIA VACCINE
    Victor Nussenzweig; Fiscal Year: 2000
    ..The specific aims are 1) to obtain by gene targeting Plasmodium berghei lines with subtle mutations in TRAP of amino acids that are presumed to coordinate metal ion, and P...
  13. BIOLOGY OF MALARIA SPOROZOITES IN THE MOSQUITO VECTOR
    KENNETH VERNICK; Fiscal Year: 2004
    ..We have made a line of Plasmodium berghei genetically transformed to produce fluorescent sporozoites...
  14. ROLE OF RF-LIKE IGM IN RESISTANCE TO MALARIA
    REUEL HOOK; Fiscal Year: 1991
    ..parasite-specific IgG subisotype, and RF-like IgM subisotypes specificity in augmentation of immunity to Plasmodium berghei in Balb/c mice...
  15. NEW TOOLS TO DEVELOP MALARIA VACCINE
    Victor Nussenzweig; Fiscal Year: 2004
    ..They have constructed rodent malaria parasites (Plasmodium berghei) expressing GFP, the green fluorescent protein...
  16. TRIOXANES FOR CHEMOTHERAPY OF MALARIA AND CANCER
    Gary Posner; Fiscal Year: 2005
    ....
  17. Chemoprevention/Therapy Using Non-Calcemic Vitamin D
    Gary Posner; Fiscal Year: 2006
    ..Collectively, these studies will provide important structure- activity insights into the cancer chemoprotective and chemotherapeutic actions and molecular biology of vitamin D3 analogs. ..
  18. Flow Cytometric Method to Analyze Micronuclei
    Dorothea Torous; Fiscal Year: 2002
    ..Similar studies are planned for analysis kits to measure MN in mouse and rat bone marrow, rat peripheral blood and also human peripheral blood. Enormous commercial opportunity exists from the sale of these kits. ..
  19. Assessing toxicity with an ex vivo erythropoiesis system
    Dorothea Torous; Fiscal Year: 2003
    ..2. MEASURE the frequency of micronuclei in ex vivo derived reticulocytes. 3. DETERMINE the responsiveness of the system to known clastogens and a model aneugen. ..
  20. REGULATION OF THE ERYTHROPOIETIN GENE
    H Bunn; Fiscal Year: 2003
    ..These studies should provide unique and highly relevant information about common mechanisms of oxygen sensing, signal transduction and gene regulation that enable organisms to adapt to hypoxia. ..
  21. A CHEMICALLY SYNTHETIC ANTI-TOXIC VACCINE AGAINST MALARI
    Louis Schofield; Fiscal Year: 2002
    ..Clinical immunity to malaria is acquired considerably earlier than anti-parasite immunity, and it is possible that this is mediated by anti-toxin mechanisms. ..
  22. Potency of cryopreserved, irradiated sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2005
    ..The project will also provide a method for cryopreserving unirradiated sporozoites that could be used to challenge volunteers by injection with P. falciparum in vaccine studies worldwide. ..
  23. STERIC-STABILIZED LIPOSOMES FOR SYSTEMIC GENE DELIVERY
    KENNETH LONGMUIR; Fiscal Year: 2002
    ..2) Gene expression, pharmokinetics, and biodistribution will be assessed for prolonged circulation times. 3) Organ tissues will be examined histologically to assess gene expression in cell types outside the vasculature. ..
  24. IMMUNOBIOLOGY OF MALARIA GLYCOSYLPHOSPHATIDYLINOSITOL
    Louis Schofield; Fiscal Year: 2001
    ..These areas are central to understanding the host/parasite interaction and the development of effective vaccines to several protozoal pathogens. ..
  25. DEVELOPMENT OF NOVEL ANTIFUNGAL ANTIPC NATURAL PRODUCTS
    Mitchell Avery; Fiscal Year: 2001
    ....
  26. Global GAS Vaccine Based On The M-Protein
    Michael Good; Fiscal Year: 2008
    ..abstract_text> ..
  27. Ca2+-Pumps in Plants: Pollen Growth and Fertilization
    Jeffrey Harper; Fiscal Year: 2008
    ..In addition, aca9 is gene level that disrupts pollen-tube/ovule and yeast provide an innovative approach calmodulin-regulated calcium pump. Our pump, PMCA4, which has been implicated ..
  28. Plasmodium carbohydrate receptors in Anopheles gambiae
    RHOEL DAVID DINGLASAN; Fiscal Year: 2008
    ..This line of experimentation will open up new avenues of research through the basic, glycobiological analysis of the mosquito midgut biology. [unreadable] [unreadable] [unreadable]..
  29. Novel Tetracycyclines as Antimalarial Drugs
    Michael Draper; Fiscal Year: 2005
    ..We anticipate that at the end of the Phase II period we will have identified several candidate molecules that are suitable for pre-clinical development. ..
  30. Dev & Testing of New Medications for Treatment of Emerging Infectious Diseases
    Mitchell Avery; Fiscal Year: 2006
    ..This allows LADDS infrastructure to inch forward now to the last stages of drug development that are achievable with academic economics. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  31. Plasmodium signaling of innate immunity in Anopheles
    Shirley Luckhart; Fiscal Year: 2007
    ....
  32. OXYGEN SENSING AND SIGNAL TRANSDUCTION
    H Bunn; Fiscal Year: 2003
    ..abstract_text> ..
  33. New Topical Therapies for Psoriasis
    Mitchell Avery; Fiscal Year: 2007
    ..The goal of this project is to develop a safe and effective new drug for topical treatment of psoriasis. [unreadable] [unreadable] [unreadable]..
  34. Cross-talk among mosquito and mammalian immune factors
    Shirley Luckhart; Fiscal Year: 2008
    ..At a more applied level, the proposed research will likely have implications for the control of malaria. [unreadable] [unreadable]..
  35. SURFACE ANTIGENS OF TREPONEMA PALLIDUM
    Wesley Van Voorhis; Fiscal Year: 2008
    ..Finally, these studies will help define the protective immune response that results after immunization with Tp92 as well as the immune response to Tp92 that occurs during infection. [unreadable] [unreadable]..
  36. Genetically Attenuated Malaria Sporozote Vaccine
    Stephen Hoffman; Fiscal Year: 2008
    ..Success in this project has the potential to lead to development of a safe, effective, attenuated malaria vaccine. [unreadable] [unreadable] [unreadable]..
  37. Lyophilization of Plasmodium falciparum sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2007
    ..Additionally, a dried vaccine may accelerate a concerted world-wide effort to eradicate this devastating disease. [unreadable] [unreadable] [unreadable]..
  38. Mosquito injection of Plasmodium sporozoites into skin
    Jerome Vanderberg; Fiscal Year: 2009
    ....
  39. Bioengineering for In Vivo-Viral Gene Delivery
    KENNETH LONGMUIR; Fiscal Year: 2007
    ..The development of this technology will have broad implications for the controlled self-assembly of a wide range of nanoparticle systems, particularly those intended for in vivo drug delivery. [unreadable] [unreadable]..
  40. Adherence of malaria-infected red cells
    Alan Cowman; Fiscal Year: 2009
    ..These studies will contribute to and increased understanding of the pathophysiology of falciparum malaria. ..