moloney murine leukemia virus

Summary

Summary: A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.

Top Publications

  1. pmc Enhanced replication and pathogenesis of Moloney murine leukemia virus in mice defective in the murine APOBEC3 gene
    Audrey Low
    Department of Molecular Biology and Biochemistry, and Cancer Research Institute, University of California, Irvine, California 92697 3905, USA
    Virology 385:455-63. 2009
  2. ncbi High-definition mapping of retroviral integration sites identifies active regulatory elements in human multipotent hematopoietic progenitors
    Claudia Cattoglio
    Istituto Scientifico H San Raffaele, Milan, Italy
    Blood 116:5507-17. 2010
  3. pmc Mutations of the RNase H C helix of the Moloney murine leukemia virus reverse transcriptase reveal defects in polypurine tract recognition
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 76:8360-73. 2002
  4. ncbi Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction
    Y Y Zhu
    CLONTECH Laboratories, Palo Alto, CA, USA
    Biotechniques 30:892-7. 2001
  5. ncbi The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus
    Debanu Das
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202 USA
    Structure 12:819-29. 2004
  6. pmc Interactions of murine APOBEC3 and human APOBEC3G with murine leukemia viruses
    Samuel J Rulli
    HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, MD 12702 1201, USA
    J Virol 82:6566-75. 2008
  7. pmc Crystal structure of the moloney murine leukemia virus RNase H domain
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 80:8379-89. 2006
  8. pmc Host cell cathepsins potentiate Moloney murine leukemia virus infection
    Pankaj Kumar
    Department of Molecular Sciences, University of Tennessee Health Sciences Center, 858 Madison Avenue, Memphis, TN 38163, USA
    J Virol 81:10506-14. 2007
  9. pmc Activation of transcription factor Nrf-2 and its downstream targets in response to moloney murine leukemia virus ts1-induced thiol depletion and oxidative stress in astrocytes
    Wenan Qiang
    University of Texas, M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    J Virol 78:11926-38. 2004
  10. pmc Moloney murine leukemia virus glyco-gag facilitates xenotropic murine leukemia virus-related virus replication through human APOBEC3-independent mechanisms
    Takayuki Nitta
    Department of Molecular Biology and Biochemistry and Cancer Research Institute, University of California, Irvine, CA 92697 3905, USA
    Retrovirology 9:58. 2012

Detail Information

Publications219 found, 100 shown here

  1. pmc Enhanced replication and pathogenesis of Moloney murine leukemia virus in mice defective in the murine APOBEC3 gene
    Audrey Low
    Department of Molecular Biology and Biochemistry, and Cancer Research Institute, University of California, Irvine, California 92697 3905, USA
    Virology 385:455-63. 2009
    ..These studies indicate that mA3 restricts replication and pathogenesis of M-MuLV in vivo...
  2. ncbi High-definition mapping of retroviral integration sites identifies active regulatory elements in human multipotent hematopoietic progenitors
    Claudia Cattoglio
    Istituto Scientifico H San Raffaele, Milan, Italy
    Blood 116:5507-17. 2010
    ..sequencing technology to build genomewide, high-definition maps of > 60 000 integration sites of Moloney murine leukemia virus (MLV)- and HIV-based retroviral vectors in the genome of human CD34(+) multipotent hematopoietic ..
  3. pmc Mutations of the RNase H C helix of the Moloney murine leukemia virus reverse transcriptase reveal defects in polypurine tract recognition
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 76:8360-73. 2002
    Both the RNase H domain of Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (RT) and Escherichia coli RNase H possess a positively charged alpha-helix (C helix) and a loop that are not present in the RNase H domains of human ..
  4. ncbi Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction
    Y Y Zhu
    CLONTECH Laboratories, Palo Alto, CA, USA
    Biotechniques 30:892-7. 2001
    ..This novel procedure uses the template-switching activity of Moloney murine leukemia virus (MMLV) reverse transcriptase to synthesize and anchor first-strand cDNA in one step...
  5. ncbi The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus
    Debanu Das
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202 USA
    Structure 12:819-29. 2004
    ..We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution...
  6. pmc Interactions of murine APOBEC3 and human APOBEC3G with murine leukemia viruses
    Samuel J Rulli
    HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, MD 12702 1201, USA
    J Virol 82:6566-75. 2008
    ..We investigated the effects of mouse APOBEC3 (mA3) and human APOBEC3G (hA3G) upon Moloney murine leukemia virus (MLV). We find that mA3 inactivates MLV but is significantly less effective against MLV than is hA3G...
  7. pmc Crystal structure of the moloney murine leukemia virus RNase H domain
    David Lim
    Integrated Program in Cellular, Molecular and Biophysical Studies, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 80:8379-89. 2006
    A crystallographic study of the Moloney murine leukemia virus (Mo-MLV) RNase H domain was performed to provide information about its structure and mechanism of action...
  8. pmc Host cell cathepsins potentiate Moloney murine leukemia virus infection
    Pankaj Kumar
    Department of Molecular Sciences, University of Tennessee Health Sciences Center, 858 Madison Avenue, Memphis, TN 38163, USA
    J Virol 81:10506-14. 2007
    The roles of cellular proteases in Moloney murine leukemia virus (MLV) infection were investigated using MLV particles pseudotyped with vesicular stomatitis virus (VSV) G glycoprotein as a control for effects on core MLV particles versus ..
  9. pmc Activation of transcription factor Nrf-2 and its downstream targets in response to moloney murine leukemia virus ts1-induced thiol depletion and oxidative stress in astrocytes
    Wenan Qiang
    University of Texas, M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    J Virol 78:11926-38. 2004
    The neuroimmunodegenerative syndrome that develops in mice infected with ts1, a mutant of Moloney murine leukemia virus, resembles human AIDS...
  10. pmc Moloney murine leukemia virus glyco-gag facilitates xenotropic murine leukemia virus-related virus replication through human APOBEC3-independent mechanisms
    Takayuki Nitta
    Department of Molecular Biology and Biochemistry and Cancer Research Institute, University of California, Irvine, CA 92697 3905, USA
    Retrovirology 9:58. 2012
    ..We examined XMRV to determine if its leader sequence contains glyco-gag activity, whether the presence of conventional gPr80Gag affects replication of XMRV, and we describe the evolution of glyco-gag-deficient MuLVs in Mus...
  11. pmc Cytoplasmic tail of Moloney murine leukemia virus envelope protein influences the conformation of the extracellular domain: implications for mechanism of action of the R Peptide
    Hector C Aguilar
    Gene Therapy Laboratories, Keck School of Medicine, University of Southern California, Los Angeles 90033, USA
    J Virol 77:1281-91. 2003
    The envelope (Env) protein of Moloney murine leukemia virus (MoMuLV) is a homotrimeric complex whose monomers consist of linked surface (SU) and transmembrane (TM) proteins cleaved from a precursor protein by a cellular protease...
  12. pmc Self-inactivating retroviral vector-mediated gene transfer induces oncogene activation and immortalization of primary murine bone marrow cells
    Marita Bosticardo
    Genetics and Molecular Biology Branch, National Human Genome Research Institute, Bethesda, Maryland, USA
    Mol Ther 17:1910-8. 2009
    ....
  13. pmc Identification of homeodomain proteins, PBX1 and PREP1, involved in the transcription of murine leukemia virus
    Sheng Hao Chao
    Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
    Mol Cell Biol 23:831-41. 2003
    ..Here we find that three CDKIs, flavopiridol, purvalanol A, and methoxy-roscovitine, block Moloney murine leukemia virus (MLV) transcription events...
  14. pmc Fidelity of two retroviral reverse transcriptases during DNA-dependent DNA synthesis in vitro
    J D Roberts
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    Mol Cell Biol 9:469-76. 1989
    We determined the fidelity of avian myeloblastosis virus and Moloney murine leukemia virus reverse transcriptases (RTs) during DNA synthesis in vitro using the M13mp2 lacZ alpha gene as a mutational target...
  15. pmc Regulatory mechanisms by which barrier-to-autointegration factor blocks autointegration and stimulates intermolecular integration of Moloney murine leukemia virus preintegration complexes
    Youichi Suzuki
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 76:12376-80. 2002
    ..We have demonstrated that barrier-to-autointegration factor (BAF) blocks the autointegration of Moloney murine leukemia virus (MoMLV) PICs in vitro. In this study, we show that BAF is an authentic component of MoMLV...
  16. ncbi TRIM28 mediates primer binding site-targeted silencing of murine leukemia virus in embryonic cells
    Daniel Wolf
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Cell 131:46-57. 2007
    b>Moloney murine leukemia virus (M-MLV) replication is restricted in embryonic carcinoma (EC) and embryonic stem (ES) cells, likely to protect the germ line from insertional mutagenesis...
  17. pmc Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins
    W Sun
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Virol 69:4941-9. 1995
    The Moloney murine leukemia virus (Mo-MLV) enhancer contains binding sites (LVb and LVc) for the ets gene family of proteins and a core site that binds the polyomavirus enhancer-binding protein 2/core-binding factor (cbf) family of ..
  18. pmc Phenylbutyric acid suppresses protein accumulation-mediated ER stress in retrovirus-infected astrocytes and delays onset of paralysis in infected mice
    Xianghong Kuang
    Department of Carcinogenesis, The University of Texas, MD Anderson Cancer Center, Science Park Research Division, Smithville, TX 78957, USA
    Neurochem Int 57:738-48. 2010
    ..previously reported that accumulation of the precursor envelope protein gPr80(env) of ts1, a mutant of Moloney murine leukemia virus (MoMuLV), in the endoplasmic reticulum (ER) of infected astrocytes, results in ER stress, oxidative ..
  19. pmc Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes
    Yujin Inayoshi
    Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8603, Japan
    J Virol 84:8250-61. 2010
    ..This study demonstrates that transcription factor Yin Yang 1 binds to Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases...
  20. pmc Bipartite signal for genomic RNA dimerization in Moloney murine leukemia virus
    Hinh Ly
    Department of Pathology, University of California, San Francisco, California 94143 0511, USA
    J Virol 76:3135-44. 2002
    ..Previous studies with Moloney murine leukemia virus (Mo-MuLV) identified two alternative DIS loci that can each independently support RNA dimerization in ..
  21. pmc Appearance of mink cell focus-inducing recombinants during in vivo infection by moloney murine leukemia virus (M-MuLV) or the Mo+PyF101 M-MuLV enhancer variant: implications for sites of generation and roles in leukemogenesis
    J K Lander
    Department of Molecular Biology and Biochemistry and Cancer Research Institute, University of California, Irvine, California 92697, USA
    J Virol 73:5671-80. 1999
    ..On the other hand, MCFs were detected in tumors from Mo+PyF101 M-MuLV s. c.-inoculated mice even though they were largely undetectable at preleukemic times. These results support a role for MCF recombinants late in disease induction...
  22. pmc RNase H activity is required for high-frequency repeat deletion during Moloney murine leukemia virus replication
    Jennifer L Brincat
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    J Virol 76:88-95. 2002
    ..To address this hypothesis, Moloney murine leukemia virus-based vectors, which were designed to test the recombination-related property of direct repeat ..
  23. pmc The p12 domain is unstructured in a murine leukemia virus p12-CA(N) Gag construct
    SAMPSON K KYERE
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Maryland, Baltimore, Maryland, United States of America
    PLoS ONE 3:e1902. 2008
    ..Here we show using nuclear magnetic resonance, that p12 is unstructured in a Moloney murine leukemia virus (MMLV) Gag fragment that includes the N-terminal domain of CA (p12-CA(N))...
  24. ncbi Mutational analysis of the N-terminus of Moloney murine leukemia virus integrase
    F Yang
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Virology 291:32-45. 2001
    ..The Moloney murine leukemia virus (M-MuLV) IN encodes a unique 45-amino-acid domain N-terminal to the HHCC motif...
  25. ncbi Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract
    Marie L Coté
    Waksman Institute, Rutgers University, Piscataway, NJ 08854 8020, USA
    J Mol Biol 330:57-74. 2003
    ..On the basis of our analysis, we suggest that the specific topology of long pure A-tracts is remarkably comparable across a wide array of biological environments...
  26. ncbi Functional reconstitution of lost activity of chemically cross-linked or mutant moloney murine leukemia virus nucleocapsid proteins by trans-complementation
    A Heldsinger
    Department of Infectious Diseases, Pfizer Global Research and Development, Ann Arbor Laboratories, Michigan 48105, USA
    Arch Virol 146:2385-400. 2001
    ..Treatment of cell free Moloney murine leukemia virus (MuLV) particles with PD156202 induced crosslinking of gag structural proteins and inhibited reverse ..
  27. ncbi Highly efficient gene transduction into the brain using high-titer retroviral vectors
    Koji Nanmoku
    Laboratory of Neural Information, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    Dev Neurosci 25:152-61. 2003
    ..Moreover, we demonstrated that predominant gene delivery into the neurons of one cortical layer can be achieved by choosing an appropriate date of retroviral infection...
  28. ncbi Differential multimerization of Moloney murine leukemia virus integrase purified under nondenaturing conditions
    Rodrigo A Villanueva
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Virology 316:146-60. 2003
    ..For Moloney murine leukemia virus (M-MuLV) IN, the limited solubility of the recombinant protein has restricted the development of ..
  29. pmc R-Peptide cleavage potentiates fusion-controlling isomerization of the intersubunit disulfide in Moloney murine leukemia virus Env
    Robin Löving
    Department of Biosciences and Nutrition, Karolinska Institute, S 141 57 Huddinge, Sweden
    J Virol 82:2594-7. 2008
    Fusion of the membrane of the Moloney murine leukemia virus (Mo-MLV) Env protein is facilitated by cleavage of the R peptide from the cytoplasmic tail of its TM subunit, but the mechanism for this effect has remained obscure...
  30. ncbi Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy
    Salima Hacein-Bey-Abina
    Laboratoire INSERM, Hopital Necker Enfants Malades, Paris, France
    N Engl J Med 346:1185-93. 2002
    ..We investigated whether infusion of autologous hematopoietic stem cells that had been transduced in vitro with the gamma(c) gene can restore the immune system in patients with severe combined immunodeficiency...
  31. ncbi Transduction of hepatocytes after neonatal delivery of a Moloney murine leukemia virus based retroviral vector results in long-term expression of beta-glucuronidase in mucopolysaccharidosis VII dogs
    Lingfei Xu
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Ther 5:141-53. 2002
    The use of Moloney murine leukemia virus (MLV)-based retroviral vectors (RV) can result in stable in vivo expression in the liver, but these vectors only transduce replicating hepatocytes...
  32. pmc Functional disruption of the moloney murine leukemia virus preintegration complex by vaccinia-related kinases
    Yasutsugu Suzuki
    Laboratory for Host Factors, Center for Emerging Virus Research, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 285:24032-43. 2010
    ..Barrier-to-autointegration factor (BAF), a cellular component of Moloney murine leukemia virus (MMLV) PICs, has been demonstrated to protect viral DNA from autointegration and stimulate the ..
  33. ncbi The conserved His8 of the Moloney murine leukemia virus Env SU subunit directs the activity of the SU-TM disulphide bond isomerase
    Kejun Li
    Department of Biosciences and Nutrition, Karolinska Institute, S 141 57 Huddinge, Sweden
    Virology 361:149-60. 2007
    ..We found that wtEnv isomerized optimally at slightly basic pH whereas the partially active mutant required higher and the inactive mutants still higher pH. This suggests that His8 directs the ionization of the CXXC thiol...
  34. ncbi Mechanisms underlying glycosylation-mediated loss of ecotropic receptor function in murine MDTF cells and implications for receptor evolution
    Hiroaki Yoshii
    Department of AIDS Research, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
    J Gen Virol 89:297-305. 2008
    A Mus dunni tail fibroblast (MDTF) cell line is highly resistant to infection by ecotropic Moloney murine leukemia virus (Mo-MLV)...
  35. ncbi Characterization of R peptide of murine leukemia virus envelope glycoproteins in syncytium formation and entry
    Y Kubo
    Department of AIDS Research, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
    Arch Virol 152:2169-82. 2007
    ..These results provide valuable information for construction of efficient MLV vectors and for understanding the retroviral entry mechanism...
  36. pmc A Moloney murine leukemia virus-based retrovirus with 4070A long terminal repeat sequences induces a high incidence of myeloid as well as lymphoid neoplasms
    Linda Wolff
    Laboratory of Cellular Oncology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    J Virol 77:4965-71. 2003
    ..This agent is a Moloney murine leukemia virus (Mo-MuLV)-based virus that has most of the U3 region of the long terminal repeat (LTR) replaced with ..
  37. pmc A mechanism misregulating p27 in tumors discovered in a functional genomic screen
    Carrie M Garrett-Engele
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    PLoS Genet 3:e219. 2007
    ..Thus, we have identified and characterized transcriptional repression of p27 by Id3 as a new mechanism decreasing p27 protein in tumors...
  38. pmc Provirus insertion in Tpl-1, an Ets-1-related oncogene, is associated with tumor progression in Moloney murine leukemia virus-induced rat thymic lymphomas
    S E Bear
    Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111
    Proc Natl Acad Sci U S A 86:7495-9. 1989
    T-cell lymphomas induced in rats by Moloney murine leukemia virus acquire increasing numbers of proviruses in their genome during tumor progression in vivo and passage of tumor cells in vitro...
  39. pmc Methylation-mediated proviral silencing is associated with MeCP2 recruitment and localized histone H3 deacetylation
    M C Lorincz
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 21:7913-22. 2001
    ..Taken together, our results suggest that MeCP2 recruitment to a methylated provirus is sufficient for transcriptional silencing, despite the presence of a remodeled enhancer...
  40. pmc An RNA structural switch regulates diploid genome packaging by Moloney murine leukemia virus
    Yasuyuki Miyazaki
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    J Mol Biol 396:141-52. 2010
    ..Recent studies with small fragments of the Moloney murine leukemia virus (MoMuLV) genome suggested that selection may be mediated by an RNA switch mechanism, in which ..
  41. pmc Phosphorylated serine residues and an arginine-rich domain of the moloney murine leukemia virus p12 protein are required for early events of viral infection
    Andrew Yueh
    Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 77:1820-9. 2003
    Mutational analyses of the p12 Gag phosphoprotein of Moloney murine leukemia virus have demonstrated its participation in both virus assembly and the early stages of infection...
  42. ncbi Structural basis of inhibitor specificity of the human protooncogene proviral insertion site in moloney murine leukemia virus (PIM-1) kinase
    Alex N Bullock
    Structural Genomics Consortium SGC, Botnar Research Centre, Oxford University, Oxford OX3 7LD, UK
    J Med Chem 48:7604-14. 2005
    ....
  43. pmc Functional characterization of a portion of the Moloney murine leukemia virus gag gene by genetic footprinting
    Marcy R Auerbach
    Department of Pathology, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 100:11678-83. 2003
    ..perform a near-saturation mutagenesis and functional analysis of 639 nucleotides in the gag region of Moloney murine leukemia virus. We report here the resulting functional map defined by eight footprints representing regions of ..
  44. ncbi Atomic resolution structure of Moloney murine leukemia virus matrix protein and its relationship to other retroviral matrix proteins
    Nico Riffel
    Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, United Kingdom
    Structure 10:1627-36. 2002
    ..The 1.0 A crystal structure of the ecotropic Gammaretrovirus Moloney murine leukemia virus (M-MuLV) matrix protein reveals the conserved topology of other retroviral matrix proteins, despite ..
  45. ncbi The single-finger nucleocapsid protein of moloney murine leukemia virus binds and destabilizes the TAR sequences of HIV-1 but does not promote efficiently their annealing
    Caroline Egelé
    Département de Pharmacologie et Physico Chimie des Interactions Cellulaires et Moléculaires, UMR 7175 CNRS, Faculte de Pharmacie, Universite Louis Pasteur, Strasbourg 1, 74, route du Rhin, 67401 Illkirch Cedex, France
    Biochemistry 46:14650-62. 2007
    ..Since NCs with a unique zinc finger are encoded by gammaretroviruses such as the canonical Moloney murine leukemia virus (MoMuLV), our objective was to characterize, by fluorescence techniques, the binding and chaperone ..
  46. pmc Role of the non-homologous DNA end joining pathway in the early steps of retroviral infection
    L Li
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 20:3272-81. 2001
    ..We propose that the double-strand ends present in unintegrated cDNA promote apoptosis, as is known to be the case for chromosomal double-strand breaks, and cDNA circularization removes the pro-apoptotic signal...
  47. ncbi A colorimetric reverse transcriptase assay optimized for Moloney murine leukemia virus, and its use for characterization of reverse transcriptases of unknown identity
    A Malmsten
    Department of Genetics and Pathology, Uppsala University, BMC, Sweden
    J Virol Methods 75:9-20. 1998
    ..transcriptase (RT) assay, reported as useful for lentivirus RTs, was optimized for the measurement of Moloney murine leukemia virus (MMuLV) RT. The optimized assay could detect 0.3 microU of MMuLV RT...
  48. pmc Identification of an H-2 Kb-presented Moloney murine leukemia virus cytotoxic T-lymphocyte epitope that displays enhanced recognition in H-2 Db mutant bm13 mice
    A J Sijts
    Department of Immunohematology and Blood Bank, University Hospital Leiden, The Netherlands
    J Virol 68:6038-46. 1994
    ..In contrast, the mink cell focus-forming virus MuLV-encoded epitope equivalent, lacking a Kb anchor residue, was not presented for CTL recognition and hence can be excluded as an important CTL epitope for mink cell focus-forming viruses...
  49. pmc Substitution of feline leukemia virus long terminal repeat sequences into murine leukemia virus alters the pattern of insertional activation and identifies new common insertion sites
    Chassidy Johnson
    Department of Microbiology and Immunology and Tulane Cancer Center, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Virol 79:57-66. 2005
    The recombinant retrovirus, MoFe2-MuLV (MoFe2), was constructed by replacing the U3 region of Moloney murine leukemia virus (M-MuLV) with homologous sequences from the FeLV-945 LTR...
  50. ncbi Reverse transcriptase of Moloney murine leukemia virus binds to eukaryotic release factor 1 to modulate suppression of translational termination
    Marianna Orlova
    Department of Biochemistry and Molecular Biophysics, Integrated Program in Cell and Molecular Biology, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Cell 115:319-31. 2003
    ..These results suggest that RT enhances suppression of termination and that the interaction of RT with eRF1 is required for an appropriate level of translational readthrough...
  51. ncbi Escherichia coli DNA polymerase III epsilon subunit increases Moloney murine leukemia virus reverse transcriptase fidelity and accuracy of RT-PCR procedures
    Bahram Arezi
    Stratagene Cloning Systems, La Jolla, CA 92037, USA
    Anal Biochem 360:84-91. 2007
    In an effort to improve reverse transcriptase (RT) fidelity, we measured the error rate of Moloney murine leukemia virus (MMLV) RT in the presence of several autonomous and DNA polymerase-associated 3'-5' exonucleases using a lacZ ..
  52. ncbi Transmission of Moloney murine leukemia virus (ts-1) by breast milk
    Joan Duggan
    Department of Medicine, Medical University of Ohio, 3000 Arlington Avenue, Toledo, OH 43614, USA
    J Gen Virol 87:2679-84. 2006
    A murine model has been developed to study maternal transmission of the temperature-sensitive Moloney murine leukemia virus (ts-1)...
  53. pmc Truncation of the human immunodeficiency virus type 1 envelope glycoprotein allows efficient pseudotyping of Moloney murine leukemia virus particles and gene transfer into CD4+ cells
    F Mammano
    Institute of Oncology, Interuniversity Center for Cancer Research, University of Padua, Italy
    J Virol 71:3341-5. 1997
    ..of HIV-1 Env proteins into viral particles formed by the Gag proteins of the widely divergent Moloney murine leukemia virus (Mo-MLV)...
  54. pmc Stabilization of TM trimer interactions during activation of moloney murine leukemia virus Env
    Mathilda Sjöberg
    Department of Biosciences and Nutrition, Karolinska Institute, S 141 57 Huddinge, Sweden
    J Virol 82:2358-66. 2008
    ..have studied the oligomer composition and stability of an intermediate and the fully activated form of Moloney murine leukemia virus (Mo-MLV) Env. Activation of Mo-MLV Env is controlled by isomerization of its intersubunit disulfide...
  55. pmc Mutational analysis of the N-terminal domain of Moloney murine leukemia virus capsid protein
    Marcy R Auerbach
    Department of Pathology, Columbia University Medical Center, New York, NY 10032, USA
    J Virol 81:12337-47. 2007
    ..To examine the role of this domain in Moloney murine leukemia virus (MoMLV) replication, we analyzed 18 insertional mutations in this region...
  56. pmc YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro
    P L Boyer
    HIV Drug Resistance Program, National Cancer Institute-FCRDC, Building 539, Frederick, MD 21702-1201, USA
    J Virol 75:6321-8. 2001
    ..However, the mutant enzymes were impaired relative to their wild-type counterparts; there appears to be steric hindrance for both 3TCTP and normal dNTPs...
  57. ncbi Mutant of Moloney murine leukemia virus reverse transcriptase exhibits higher resistance to common RT-qPCR inhibitors
    Bahram Arezi
    Agilent Technologies, Stratagene Products Division, La Jolla, CA 92037, USA
    Anal Biochem 400:301-3. 2010
    Inhibitor resistance of several commercial Moloney murine leukemia virus reverse transcriptase (MMLV RT) enzymes was investigated...
  58. ncbi Atomic force microscopy investigation of wild-type Moloney murine leukemia virus particles and virus particles lacking the envelope protein
    Y G Kuznetsov
    Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697 3900, USA
    Virology 323:189-96. 2004
    b>Moloney murine leukemia virus (M-MuLV) lacking the gene for the envelope glycoprotein (env(-)) was produced in NIH 3T3 cells and investigated using atomic force microscopy (AFM)...
  59. pmc Retroviral integration process in the human genome: is it really non-random? A new statistical approach
    Alessandro Ambrosi
    University Centre for Statistics in the Biomedical Sciences, Universita Vita Salute San Raffaele, Milan Italy
    PLoS Comput Biol 4:e1000144. 2008
    ..The statistical goodness of fit test shows the suitability of this distribution to the observed data. Our statistical analysis confirms the preference of MLV-based vectors to integrate in promoter-proximal regions...
  60. ncbi Mutational analysis of the R peptide cleavage site of Moloney murine leukaemia virus envelope protein
    Yoshinao Kubo
    Molecular Cell Science Laboratory, RIKEN The Institute of Physical and Chemical Research, Hirosawa 2 1, Wako, Saitama 351 0198, Japan
    J Gen Virol 84:2253-7. 2003
    ..This result strongly supports that R peptide cleavage is required for virus entry...
  61. pmc Secondary structure of the mature ex virio Moloney murine leukemia virus genomic RNA dimerization domain
    Cristina Gherghe
    Department of Chemistry, University of North Carolina, A008 Kenan Labs, CB 3290, Chapel Hill, NC 27599 3290, USA
    J Virol 84:898-906. 2010
    ..These experiments support well-defined structural models for an authentic dimerization domain and also emphasize that many features of mature genomic RNA dimers can be reproduced in vitro using properly designed, simplified RNAs...
  62. pmc Moloney murine leukemia virus-induced myeloid tumors in adult BALB/c mice: requirement of c-myb activation but lack of v-abl involvement
    G L Shen-Ong
    Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892
    J Virol 61:3721-5. 1987
    ..Myelomonocytic tumors with helper Moloney murine leukemia virus clonally inserted into the c-myb locus were observed in about 10% of pristane-primed BALB/c mice ..
  63. pmc Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production
    Margaret Q Wang
    Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
    J Biol 3:4. 2003
    ..Recent evidence suggests that Gag may co-opt the host cell's endocytic machinery to facilitate retroviral assembly and release...
  64. ncbi Moloney murine leukemia virus decay mediated by retroviral reverse transcriptase degradation of genomic RNA
    Monica Casali
    Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Shriners Burns Hospital, Harvard Medical School, Boston, MA 02114, USA
    Virology 380:91-8. 2008
    ....
  65. pmc Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus
    A D Miller
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    J Virol 65:2220-4. 1991
    ..hybrid retrovirus packaging cell lines that express the gibbon ape leukemia virus env and the Moloney murine leukemia virus gag-pol proteins...
  66. pmc Infectivity of Moloney murine leukemia virus defective in late assembly events is restored by late assembly domains of other retroviruses
    B Yuan
    Integrated Program in Cellular, Molecular and Biophysical Studies, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Virol 74:7250-60. 2000
    The p12 region of the Moloney murine leukemia virus (M-MuLV) Gag protein contains a PPPY motif important for efficient virion assembly and release...
  67. pmc Dynamic analysis of proviral induction and De Novo methylation: implications for a histone deacetylase-independent, methylation density-dependent mechanism of transcriptional repression
    M C Lorincz
    Fred Hutchinson Cancer Research Center, University of Washington School of Medicine, Seattle, Washington, USA
    Mol Cell Biol 20:842-50. 2000
    ..MEL cells were infected with a Moloney murine leukemia virus encoding the green fluorescent protein (GFP), and single-copy, silent proviral clones were treated ..
  68. ncbi T lymphocytes as targets of gene transfer with Moloney-type retroviral vectors
    F A Ayuk
    University Hospital Eppendorf, Martinistr. 52, 20251 Hamburg, Germany
    Curr Gene Ther 1:325-37. 2001
    ..More attention is thus being focused on the ability of gene-modified cells to carry out innate as well as conferred functions in vivo and the influence of culture conditions, retroviral vector and host response thereon...
  69. pmc The role of template-primer in protection of reverse transcriptase from thermal inactivation
    Gary F Gerard
    Invitrogen Corporation, 7335 Executive Way, Frederick, MD 21704, USA
    Nucleic Acids Res 30:3118-29. 2002
    We compared the thermal stabilities of wild-type recombinant avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) with those of mutants of the recombinant enzymes lacking RNase H activity...
  70. pmc Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase itch
    Joshua A Jadwin
    Laboratory of Molecular Microbiology, NIAID, NIH, 4 Center Dr, Bethesda, MD 20892, USA
    J Virol 84:704-15. 2010
    b>Moloney murine leukemia virus (MoMLV) Gag utilizes its late (L) domain motif PPPY to bind members of the Nedd4-like ubiquitin ligase family...
  71. ncbi Genome-wide retroviral insertional tagging of genes involved in cancer in Cdkn2a-deficient mice
    Anders H Lund
    Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Nat Genet 32:160-5. 2002
    ..We infected Cdkn2a(-/-) mice with Moloney murine leukemia virus (MoMuLV) to screen for loci that can participate in tumorigenesis in collaboration with loss of the ..
  72. ncbi Transduction of Schistosoma mansoni by vesicular stomatitis virus glycoprotein-pseudotyped Moloney murine leukemia retrovirus
    Kristine J Kines
    Department of Tropical Medicine and Center for Infectious Diseases, Tulane University, Health Sciences Center, New Orleans, LA 70112, USA
    Exp Parasitol 112:209-20. 2006
    ..Together these results indicated productive transduction by VSVG pseudotyped MMLV of cultured schistosomes, and suggest a tractable route forward towards heritable schistosome transgenesis...
  73. ncbi A glycosylation-defective variant of the ecotropic murine retrovirus receptor is expressed in rat XC cells
    Yoshinao Kubo
    Molecular Cell Science Laboratory, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, Japan
    Virology 303:338-44. 2002
    ..However, xcCAT1-expressing CHO cells did not fuse with 293T cells expressing R+ Env, indicating that xcCAT1 expression is not sufficient to induce the XC cell-specific characteristic of forming syncytia in response to R+ Env...
  74. ncbi NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus
    Victoria D'Souza
    Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    J Mol Biol 337:427-42. 2004
    The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly...
  75. pmc Human immunodeficiency virus type 1 genetic recombination is more frequent than that of Moloney murine leukemia virus despite similar template switching rates
    Adewunmi Onafuwa
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    J Virol 77:4577-87. 2003
    ....
  76. ncbi Complex determinants within the Moloney murine leukemia virus capsid modulate susceptibility of the virus to Fv1 and Ref1-mediated restriction
    J Wesley Ulm
    Department of Genetics, Harvard Medical School, Children s Hospital, Harvard Gene Therapy Initiative, 4 Blackfan Circle, Boston, MA 02115, USA
    Virology 363:245-55. 2007
    ..These studies shed further light on the complex determinants within the viral CA gene product which control the susceptibility of murine and human cells to retroviral infection...
  77. pmc Interaction of Moloney murine leukemia virus matrix protein with IQGAP
    Juliana Leung
    Integrated Program in Cellular, Molecular, and Biophysical Studies, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    EMBO J 25:2155-66. 2006
    The matrix protein (MA) of the Moloney murine leukemia virus (M-MuLV) was found to interact with IQGAP1, a prominent regulator of the cytoskeleton...
  78. pmc Kinetic analyses of the surface-transmembrane disulfide bond isomerization-controlled fusion activation pathway in Moloney murine leukemia virus
    Michael Wallin
    Department of Biosciences at Novum, Karolinska Institute, S 141 57 Huddinge, Sweden
    J Virol 79:13856-64. 2005
    The surface (SU) and transmembrane (TM) subunits of Moloney murine leukemia virus (Mo-MLV) Env are disulfide linked. The linking cysteine in SU is part of a conserved CXXC motif in which the other cysteine carries a free thiol...
  79. pmc Two distinct Moloney murine leukemia virus RNAs produced from a single locus dimerize at random
    Jessica A Flynn
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    Virology 344:391-400. 2006
    ..For Moloney murine leukemia virus (MLV), co-packaged RNAs are not randomly selected from among all packaging-competent RNAs, but instead ..
  80. ncbi A Moloney murine leukemia virus driven by the Jaagsiekte sheep retrovirus enhancers shows enhanced specificity for infectivity in lung epithelial cells
    Kathleen McGee-Estrada
    Department of Molecular Biology and Biochemistry and Cancer Research Institute, University of California, Irvine, CA, 92697 3905, USA
    Virus Genes 31:257-63. 2005
    ..the tissue specificity, we inserted the JSRV enhancer sequences from the U3 region of the LTR into a Moloney murine leukemia virus (M-MuLV) LTR lacking its own enhancer sequences, to give the chimeric LTR DeltaMo + JS...
  81. ncbi Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and Moloney murine leukaemia virus
    Kiyoshi Yasukawa
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto, Japan
    J Biochem 143:261-8. 2008
    ..Thermodynamic analysis indicates that thermal inactivation of AMV RT and MMLV RT is due to the large entropy change of activation for thermal inactivation...
  82. pmc A host-guest approach for determining drug-DNA interactions: an example using netropsin
    Kristie D Goodwin
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, IN 46202, USA
    Nucleic Acids Res 33:4106-16. 2005
    ..novel host-guest approach to study drug-DNA interactions in which the host, the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) is co-crystallized with a DNA oligonucleotide guest in the presence ..
  83. pmc Evidence for preferential copackaging of Moloney murine leukemia virus genomic RNAs transcribed in the same chromosomal site
    Sergey A Kharytonchyk
    Laboratory of Cellular and Molecular Immunology, Institute of Hematology and Blood Transfusion, 223059 Minsk, Republic of Belarus
    Retrovirology 2:3. 2005
    ..The reason for these differences is not clear. One possibility is that these retroviruses may differ in their ability to copackage genomic RNAs produced at different chromosomal loci...
  84. ncbi Activation of c-myb by 5' retrovirus promoter insertion in myeloid neoplasms is dependent upon an intact alternative splice donor site (SD') in gag
    Jean Marie Ramirez
    Laboratoire Infections Retrovirales et Signalisation Cellulaire, CNRS UMR5121, UMI, IFR122, Montpellier, France
    Virology 330:398-407. 2004
    ..Importantly, there was a complete lack of myeloid tumors associated with 5' insertional mutagenic activation of c-myb, suggesting the specific requirement of the SD' site in this mechanism...
  85. pmc LAP2alpha and BAF collaborate to organize the Moloney murine leukemia virus preintegration complex
    Youichi Suzuki
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    EMBO J 23:4670-8. 2004
    ..Depletion of LAP2alpha significantly inhibited viral replication. Our results demonstrate a critical contribution of LAP2alpha to the nucleoprotein organization of the PIC and to viral replication...
  86. pmc Nonrandom dimerization of murine leukemia virus genomic RNAs
    Jessica A Flynn
    Department of Microbiology and Immunology, 1150 W Medical Center Drive, Room 5641, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    J Virol 78:12129-39. 2004
    ..Here, virion RNA homo- and heterodimerization were examined for Moloney murine leukemia virus (MLV) using nondenaturing Northern blotting and a novel RNA dimer capture assay...
  87. ncbi Yeast system as a model to study Moloney murine leukemia virus integrase: expression, mutagenesis and search for eukaryotic partners
    Jorge Vera
    Programa de Virologia, ICBM, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile
    J Gen Virol 86:2481-8. 2005
    b>Moloney murine leukemia virus (M-MuLV) integrase (IN) catalyses the insertion of the viral genome into the host chromosomal DNA...
  88. ncbi Genistein, a protein tyrosine kinase inhibitor, suppresses the fusogenicity of Moloney murine leukemia virus envelope protein in XC cells
    Y Kubo
    Molecular Cell Science Laboratory, RIKEN, Saitama, Japan
    Arch Virol 148:1899-914. 2003
    ..This result indicates that protein tyrosine kinase activity is associated with the high sensitivity of XC cells to ecotropic Env-induced syncytium formation...
  89. pmc Host proteins interacting with the Moloney murine leukemia virus integrase: multiple transcriptional regulators and chromatin binding factors
    Barbara Studamire
    Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, Hammer Health Sciences Center, Room 1310c, New York 10032, USA
    Retrovirology 5:48. 2008
    ..Significant questions remain regarding the influence of host proteins on the selection of target sites, on the repair of integration intermediates, and on the efficiency of integration...
  90. ncbi Retroviral insertional mutagenesis: tagging cancer pathways
    Harald Mikkers
    Division of Molecular Genetics and Centre of Biomedical Genetics, Netherlands Cancer Institute 1066 CX, Amsterdam, The Netherlands
    Adv Cancer Res 88:53-99. 2003
    Slow transforming retroviruses, such as the Moloney murine leukemia virus (M-MuLV), induce tumors upon infection of a host after a relatively long latency period...
  91. ncbi An essential role for the His-8 residue of the SDF-1alpha-chimeric, tropism-redirected Env protein of the Moloney murine leukemia virus in regulating postbinding fusion events
    Masumi Katane
    Molecular Cell Science Laboratory, Discovery Research Institute, RIKEN, Wako, Saitama 351 0198, Japan
    J Gene Med 6:260-7. 2004
    ..Previously, we reported that a Moloney murine leukemia virus (MLV) retroviral vector containing a human stromal-derived factor-1alpha (SDF-1alpha)-chimeric ..
  92. ncbi Ligand presentation on a synthetic flexible hinge in Moloney murine leukemia virus SU supports entry via a heterologous receptor
    Shervin Bahrami
    Department of Molecular Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
    Virology 363:303-9. 2007
    The envelope protein of Moloney murine leukemia virus mediates entry into mCAT-expressing cells. Attempts to change its receptor usage through the insertion of ligands at various sites have been met with varying success...
  93. ncbi Low efficiency of the Moloney murine leukemia virus reverse transcriptase during reverse transcription of rare t(8;21) fusion gene transcripts
    John Curry
    Department of Immunology, Molecular and Cellular Biology, University of California, Berkeley 94720 3200, USA
    Biotechniques 32:768, 770, 772, 754-5. 2002
    ..The efficiency is shown to be directly proportional to RNA template levels. The Moloney murine leukemia virus (MMLV) reverse transcriptase enzyme's conversion efficiency was calculated to be approximately 20%...
  94. ncbi Localization of actin in Moloney murine leukemia virus by immunoelectron microscopy
    M V Nermut
    National Institute for Biological Standards and Control, South Mimms, Hertfordshire, EN6 3QG, United Kingdom
    Virology 260:23-34. 1999
    Immunoelectron microscopy was used to detect actin in wild-type (wt) Moloney murine leukemia virus (MoMuLV) and in virus-like particles (VLP) produced by recombinant Semliki Forest virus expressing only the MoMuLV gag polyprotein...
  95. ncbi Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging
    M van Lohuizen
    Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam
    Cell 65:737-52. 1991
    ....
  96. pmc Combined infection by Moloney murine leukemia virus and a mink cell focus-forming virus recombinant induces cytopathic effects in fibroblasts or in long-term bone marrow cultures from preleukemic mice
    Q X Li
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92717
    J Virol 64:3701-11. 1990
    We described previously a preleukemic state in mice inoculated with Moloney murine leukemia virus (M-MuLV) characterized by generalized hematopoietic hyperplasia in the spleen...
  97. pmc Characterization of pal-1, a common proviral insertion site in murine leukemia virus-induced lymphomas of c-myc and Pim-1 transgenic mice
    B Scheijen
    Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam
    J Virol 71:9-16. 1997
    Insertional mutagenesis with Moloney murine leukemia virus (MoMLV) in c-myc and Pim-1 transgenic mice permits the identification of oncogenes that collaborate with the transgenes in lymphomagenesis...
  98. pmc Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: implications for retroviral assembly mechanisms
    M Yeager
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 95:7299-304. 1998
    ..to examine the native structure of immature, protease-deficient (PR-) and mature, wild-type (WT) Moloney murine leukemia virus (MuLV)...
  99. ncbi Frequency of direct repeat deletion in a human immunodeficiency virus type 1 vector during reverse transcription in human cells
    W An
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0620, USA
    Virology 286:475-82. 2001
    ..The results indicated that the error rate and the frequency of repeat deletion of HIV-1 were similar to those of MuLV...
  100. pmc Effects of limiting homology at the site of intermolecular recombinogenic template switching during Moloney murine leukemia virus replication
    J K Pfeiffer
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0620, USA
    J Virol 75:11263-74. 2001
    A Moloney murine leukemia virus-based single-replication-cycle assay was developed to study the effects of limiting the extent of template and primer strand complementarity on recombinogenic template switching...
  101. pmc Assembly and catalysis of concerted two-end integration events by Moloney murine leukemia virus integrase
    F Yang
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey-- Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Virol 75:9561-70. 2001
    ..An in vitro concerted two-end integration reaction catalyzed by the Moloney murine leukemia virus (M-MuLV) integrase (IN) was used to investigate the binding and coordination of the two viral DNA ends...

Research Grants62

  1. The role of XMRV, a newly discovered human retrovirus, in cancer pathogenesis
    Ila R Singh; Fiscal Year: 2013
    ..with protein composition and particle ultrastructure highly similar to another gammaretrovirus, Moloney murine leukemia virus (MoMLV)...
  2. MOUSE MODELS FOR UNDERSTANDING HOST RESPONES TO INTRACELLULAR PATHOGENS
    Ellen A Robey; Fiscal Year: 2010
    ..virus) and other pathogens (Murine &Human cytomegalovirus, Murine herpes virus 68, Moloney Murine Leukemia Virus, Kaposi's Sarcoma Herpes Virus, Mouse Hepatitis Virus, Mouse Adeno Virus, Polyomavirus)...
  3. Directed Cell Fusion for the Treatment of Myocardial Infarction
    Brenda M Ogle; Fiscal Year: 2010
    ..We propose to co-opt the unique fusion capabilities of the Moloney murine leukemia virus (MuLV) to develop a method for increasing the frequency of fusion between stem cells and ..
  4. Integration of Murine Retroviral Vectors
    Monica J Roth; Fiscal Year: 2013
    ..proposal develops and extends preliminary structural analysis of the N-terminal domain (NTD) of the Moloney Murine Leukemia Virus Integrase (M-MuLV IN) protein to define the function of this domain within the preintegrative complex...
  5. GENETICS OF MYELODYSPLASTIC SYNDROMES
    Matthew J Walter; Fiscal Year: 2013
    ..to leukemia development, we will infect Hspa9+/- or wild-type littermate mice with a modified Moloney murine leukemia virus (MOL4070LTR retrovirus, a well established AML model) and monitor mice for the development of AML...
  6. NMR STUDIES OF RETROVIRAL NUCLEIC ACID BINDING PROTEINS
    MICHAEL FINLEY SUMMERS; Fiscal Year: 2013
    ..During the current funding period, we obtained strong in vivo and in vitro evidence that the Moloney Murine Leukemia Virus (MLV) uses an RNA structural switch mechanism, in which high affinity nucleocapsid (NC - the protein ..
  7. Modeling HTLV-1 infection and cellular immune response in HLA-A2-transgenic mice
    Zafar K Khan; Fiscal Year: 2012
    ..Additionally the results of these studies will shed light on the dynamics of immune cell interactions during chronic viral infections such as HTLV-1, HIV-1, hepatitis virus and herpes simplex virus. ..
  8. CONSTRUCTION AND ANALYSIS OF RETROVIRUS MUTANTS
    STEPHEN PAINE GOFF; Fiscal Year: 2013
    This proposal describes the genetic analysis of the Gag and Pol proteins of the Moloney murine leukemia virus and of the cellular host proteins with which they interact...
  9. Retroviral Integration &HDAC inhibitors
    Monica J Roth; Fiscal Year: 2012
    ..This new area of investigation is based on the observation that mutations within the Moloney murine leukemia virus integrase (M-MuLV IN) proteins can be compensated by the presence of histone deacetylase (HDAC) ..
  10. Defining genetic pathways of plasma-cell neoplasia
    Siegfried Janz; Fiscal Year: 2013
    ..The studies in Aim 1 will rely on a modified Moloney murine leukemia virus, MOL4070LTR...
  11. Stem cell gene therapy for canine hemophilia
    Friedrich Schuening; Fiscal Year: 2002
    ..Transduction efficiency into hematopoietic stem cells of large animals and humans using Moloney murine leukemia virus (MoMLV) vectors has been too low so far to achieve a therapeutic effect...
  12. The Enzymology of M-MuLV and HIV Replication
    JAMES CHAMPOUX; Fiscal Year: 2009
    ..of hypotheses relating to the molecular details of human immunodeficiency virus type 1 (HIV-1) and Moloney murine leukemia virus (M-MuLV) replication...
  13. ANALYSIS OF CELLULAR ONCOGENES IN VIRUS-INDUCED TUMORS
    David Steffen; Fiscal Year: 1992
    ..Using thymic lymphomas induced in rats by Moloney murine leukemia virus (MoMLV), new oncogenes will be identified and characterized and the mechanism by which normal proto-..
  14. ALPHA-1-PROTEASE INHIBITOR DEFICIENCY--GENE THERAPY
    Jerry Brown; Fiscal Year: 1991
    ..Three approaches will be tested to modify the Moloney murine leukemia virus (MoMLV) envelope protein to contain appropriate ligands for interaction with the ASGP receptor: 1) ..
  15. IDURONIDASE GENE TRANSFER INTO HEMATOPOIETIC CELLS
    Friedrich Schuening; Fiscal Year: 2004
    ..strategies with low toxicity to increase engraftment of hematopoietic stem cells transduced with Moloney murine leukemia virus (MoMLV) vectors transduced marrow cells in the absence of conditioning or infusions after partial ..
  16. TRANSDUCTION OF HUMAN HEMATOPOIETIC STEM CELLS
    Gay Crooks; Fiscal Year: 2001
    ..The low efficiency of transduction using Moloney Murine Leukemia Virus (MoMuLV) based vectors is assumed to be secondary to non- integration of provirus into the genomes of ..
  17. MECHANISMS OF RETROVIRUS INDUCED T AND NEURAL CELL DEATH
    Paul Wong; Fiscal Year: 2003
    ..is to understand the pathogenic mechanism of neuroimmunodegeneration (NID) induced by a mutant of the Moloney murine leukemia virus, ts1...
  18. Control of retrovirus CNS disease by redox modulation
    Paul K Wong; Fiscal Year: 2010
    ..In our model of HAD, FVB/N mice are infected with a mutant of the Moloney murine leukemia virus, called te1, which causes progressive neuroimmunodegeneration that is in many ways similar to that of ..
  19. PATHOGENESIS OF RETROVIRAL INFECTION OF THE CNS
    Clayton Wiley; Fiscal Year: 1993
    ..Nevertheless, with the proliferation of models and development temperature sensitive mutants of Moloney murine leukemia virus, there has been a profusion of theories regarding retroviral mediated neurologic disease...
  20. FAT NUCLEOSIDES AND NUCLEOTIDES
    RAMACHANDRA HOSMANE; Fiscal Year: 1990
    ..and the resulting homopolymer templates will be evaluated for substrate/inhibitory activity against Moloney murine leukemia virus (M-MuLV) reverse transcriptase...
  21. GENOMIC DETERMINANTS OF MURINE LEUKEMIA VIRUS
    Alice Telesnitsky; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): The proposed research examines Moloney murine leukemia virus as a model system for testing determinants and outcomes of general and specific features of retroviral genome structure...
  22. Transcriptional repression in leukemia
    H Grimes; Fiscal Year: 2007
    ..GFI1 was originally identified as a frequent target of Moloney murine leukemia virus insertion in rat and mouse genomes...
  23. ETIOLOGIC ROLE OF TNF/FAS ON AN AIDS-LIKE CNS DISEASE
    Paul Wong; Fiscal Year: 2000
    ..design relies on the use of a murine model of neurodegeneration in which infection of a mutant form of Moloney Murine Leukemia Virus (MoMuLV) induces a progressive neuroimmunodegenerative disease (NID) that has some clinical and ..
  24. ENZYMOLOGY OF M-MULV AND HIV REPLICATION
    JAMES CHAMPOUX; Fiscal Year: 1999
    ..is to understand in molecular detail all of the steps in the process of reverse transcription by both Moloney murine leukemia virus (M- MuLV) and human immunodeficiency virus type 1 (HIV-1)...
  25. INTRAMARROW GENE TRANSFER IN NEONATES
    Bruce Bunnell; Fiscal Year: 2003
    ..The investigators will explore the most effective vector system (Moloney murine leukemia virus {MLV} versus HIV-1-derived lentivirus), the effects of cytokines on the in vivo transduction ..
  26. SPECIALIZED CANCER CENTER (CORE) GRANT
    Maurice Green; Fiscal Year: 1980
    ..Interrelationships between the Moloney murine leukemia virus RNase H-I and -III will be further established, and the possible role of RNase H in proviral DNA ..
  27. GENOMIC DETERMINANTS OF MURINE LEUKEMIA VIRUS
    Alice Telesnitsky; Fiscal Year: 2000
    ..proposal examines general and specific parameters that influence retroviral genome structure, using Moloney murine leukemia virus as a model system...
  28. Genetic targeting of proviral integration in stem cells
    Peter Kurre; Fiscal Year: 2009
    ..preferences for transcriptionally active genomic sites and reports of insertional mutagenesis after Moloney murine leukemia virus vector transduction of stem cells have highlighted the need to more fully investigate the biology of ..
  29. MODIFIED RETROVIRAL VECTORS FOR HSC GENE THERAPY
    Donald Kohn; Fiscal Year: 2000
    ..retroviral LTRs, MND and MTD, showed significantly improved expression, compared to the standard Moloney Murine Leukemia virus (MLV) LTR, in murine ES cells and in murine hematopoietic and lymphoid cells...
  30. MECHANISM OF RETROVIRAL INTEGRATION
    MONICA ROTH; Fiscal Year: 2000
    ..Through the insertion of nuclear localization sequences into targeted viral proteins, the ability of the murine based retro viruses to infect non-replicating cells will be examined. ..
  31. CELL BIOLOGY OF SURFACTANT PROTEIN B (SP B)
    Daphne Demello; Fiscal Year: 2000
    ..an SP-B deficient cell line by infection with our SP-B anti-sense retroviral vector derived from Moloney murine leukemia virus; 2) compare in normal and SP-B deficient Type II cells (SP-B+, & SP-B-) the synthesis, assembly, ..
  32. GENE TRANSFER FOR SUPPRESSION OF THE TUMOR PHENOTYPE
    Theodore Friedmann; Fiscal Year: 1993
    ..Our specific aims are: 1. to modify and improve Moloney murine leukemia virus (MoMLV)-based vectors, 2...
  33. GENETICALLY ALTERED RTS PREPARE BETTER CDNA LIBRARIES
    Vinayaka Prasad; Fiscal Year: 1999
    ..We propose to utilize mutants of human immunodeficiency virus type 1 (HIV-1) and Moloney murine leukemia virus (MoMuLV) RT that display improved processivity and/or better polymerase fidelity in the construction ..
  34. FUNCTIONS OF THE BROTHER/CBFB PROTO-ONCOPROTEIN FAMILY
    John Gergen; Fiscal Year: 2001
    ..This factor was first studied in its capacity as a regulator of Moloney murine leukemia virus and Polyoma DNA tumor virus function...
  35. Identification of Host Genes for Retroviral Replication
    Suzanne Sandmeyer; Fiscal Year: 2006
    ..to survey a subset of nineteen genes for effects on replication of a simple mammalian retrovirus, Moloney murine leukemia virus. The goals of this application are: 1) Target gene function will be disrupted by shRNA...
  36. RECOMBINANT RETROVIRUS FOR SCREENING OF ANTI-AIDS DRUGS
    CHAMER WEI; Fiscal Year: 1990
    ..This proposed research is to construct a recombinant Moloney murine leukemia virus carrying HIV reverse transcriptase...
  37. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  38. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  39. Mechanism of Redox Regulation by Ape1/Ref-1
    Millie M Georgiadis; Fiscal Year: 2010
    ..We propose here to elucidate the mechanism of the redox function of Ape1 and explore its role in tumor cell response following treatment with DNA damaging agents. ..
  40. Structural studies of protein-nucleic acid interactions
    Millie Georgiadis; Fiscal Year: 2005
    ..relevant and novel nucleic acid complexes with three proteins including the human protein Tap, Moloney murine leukemia virus reverse transcriptase (MMLV RI), and an N-terminal fragment of MMLV RT...
  41. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  42. ANTI VIRAL THERAPEUTIC FOR EBV MALIGNANCIES
    DOUGLAS FALLER; Fiscal Year: 2002
    ..abstract_text> ..
  43. Regulatable Gene Transfer for Spinal Cord Injury
    Armin Blesch; Fiscal Year: 2010
    ..If successful, these studies will establish practical strategies for growth factor delivery to the injured spinal cord that can lead to the development of novel therapies for spinal cord injury. ..
  44. Chemokines in pathogenesis of Experimental Arthritis
    SEEMA SINGH AHUJA; Fiscal Year: 2010
    ..Thus information obtained from proposed studies which explore how CCR2 modulates arthritis will have significant impact on potential therapeutic strategies to treat this chronic deblitating disease. ..
  45. Development of a Ras-Targeted Cancer Therapeutic
    DOUGLAS FALLER; Fiscal Year: 2004
    ..Finally, we will employ, and continuously refine, a new molecular strategy (a pharmacophore model) in the search for more active and more specific inducers of Ras-mediated apoptosis. ..
  46. CANCER RESEARCH CENTER
    DOUGLAS FALLER; Fiscal Year: 2002
    ..abstract_text> ..
  47. GENE THERAPY FOR BLOOD PROTEIN DEFICIENCIES
    KATHERINE PONDER; Fiscal Year: 2005
    ..Success in this project might lead to a safe, effective, and permanent therapy for Hemophilia B. ..
  48. Arsenic Trioxide and Vitamin C in AML
    Dan Douer; Fiscal Year: 2005
    ..In summary, this combination may have higher benefit/risk ratio in non-APL AML patients resulting in an overall better outcome with less toxicity than conventional chemotherapy. ..
  49. Chemokine System in DC and Lymphocyte Function
    SEEMA AHUJA; Fiscal Year: 2005
    ....
  50. CONTROL OF CELL GROWTH BY SHORT-CHAIN FATTY ACIDS
    DOUGLAS FALLER; Fiscal Year: 2004
    ..abstract_text> ..
  51. Oral Therapeutic for Inducing Fetal Hemoglobin
    DOUGLAS FALLER; Fiscal Year: 2003
    ..Upon completion of preclinical studies, Investigational New Drug status will be obtained and the new therapeutic agent will be tested in human clinical trials. ..
  52. Center for Fetal Gene Transfer for HLB Diseases
    Alice Tarantal; Fiscal Year: 2005
    ..abstract_text> ..
  53. Antitumor Activity of Inducible Nitric Oxide Synthase
    Keping Xie; Fiscal Year: 2005
    ..Collectively, all of these insightful studies will be crucial to effectively targeting the iNOS/NO pathway in designing novel therapeutic strategies or improving current therapeutic modalities against cancer. ..
  54. Gene Therapy for HPV-Associated Malignancies
    Andre Lieber; Fiscal Year: 2006
    ..Finally, even if the rate of tumor transduction with the viral vector is limited, an anti-tumor immune response will lead to lysis of tumor cells that were originally not transduced. ..
  55. Mesenchymal Stem Cells for Fetal Gene Delivery
    Alice Tarantal; Fiscal Year: 2006
    ..abstract_text> ..
  56. Structure Analysis of Aquaporin Membrane Channels
    Mark Yeager; Fiscal Year: 2006
    ..In addition, this project will provide insight into the molecular basis for channel regulation and will contribute to the general principles that govern conformational changes of membrane proteins. ..
  57. IMPROVED METHODS FOR IN VIVO HEPATIC GENE THERAPY
    KATHERINE PONDER; Fiscal Year: 2006
    ..hepatocytes and result in long-term and therapeutic levels of expression in rodents, both the older Moloney murine leukemia virus (MLV) vectors as well as the newer lentiviral vectors only efficiently transduce dividing hepatocytes...
  58. IL-12-Faciliated Hematopoietic Recovery Following Myeloablative Therapy
    Dan Douer; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  59. MOLECULAR BIOLOGY OF JAAGSIEKTE SHEEP RETROVIRUS
    Hung Fan; Fiscal Year: 2003
    ..These experiments will provide insight into JSRV-induced lung cancer in sheep, and possibly into human BAC. ..
  60. Evaluation of vectors based on group B adenoviruses
    Andre Lieber; Fiscal Year: 2009
    ..These studies will be performed in transgenic mice that express B-group Ad receptors. Based on this, we will select representative vectors and parameters and repeat biodistribution and safety studies in baboons. ..
  61. Phase I study of 8-Cl-adenosine in CLL (IND 68,229)
    Varsha Gandhi; Fiscal Year: 2010
    ..The investigators state that these Aims will establish utility of 8-Cl-Ado in CLL, provide a dose for Phase 2 study, and knowledge for optimal use of this agent alone and in combination. ..