chick embryo

Summary

Top Publications

  1. ncbi Requirement of vascular integrin alpha v beta 3 for angiogenesis
    P C Brooks
    Department of Immunology, Scripps Research Institute, La Jolla, CA 92037
    Science 264:569-71. 1994
  2. ncbi Vertebrate neurogenesis is counteracted by Sox1-3 activity
    Magdalena Bylund
    Ludwig Institute of Cancer Research, Karolinska Institute, Box 240, SE 171 77 Stockholm, Sweden
    Nat Neurosci 6:1162-8. 2003
  3. ncbi Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity
    S Arber
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
    Neuron 23:659-74. 1999
  4. ncbi The avian Z-linked gene DMRT1 is required for male sex determination in the chicken
    Craig A Smith
    Murdoch Children s Research Institute and Department of Paediatrics, The University of Melbourne, Royal Children s Hospital, Melbourne, Victoria 3052, Australia
    Nature 461:267-71. 2009
  5. ncbi Pax6 is required for the multipotent state of retinal progenitor cells
    T Marquardt
    Max Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Am Fassberg 11, D 37077, Gottingen, Germany
    Cell 105:43-55. 2001
  6. ncbi Opposing FGF and retinoid pathways control ventral neural pattern, neuronal differentiation, and segmentation during body axis extension
    Ruth Diez del Corral
    Division of Cell and Developmental Biology, Faculty of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
    Neuron 40:65-79. 2003
  7. ncbi Sonic hedgehog mediates the polarizing activity of the ZPA
    R D Riddle
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Cell 75:1401-16. 1993
  8. ncbi Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein
    A Vortkamp
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 273:613-22. 1996
  9. ncbi Control of cell behavior during vertebrate development by Slug, a zinc finger gene
    M A Nieto
    Instituto Cajal, Madrid, Spain
    Science 264:835-9. 1994
  10. ncbi Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10021, USA
    Nat Biotechnol 25:1468-75. 2007

Detail Information

Publications333 found, 100 shown here

  1. ncbi Requirement of vascular integrin alpha v beta 3 for angiogenesis
    P C Brooks
    Department of Immunology, Scripps Research Institute, La Jolla, CA 92037
    Science 264:569-71. 1994
    ..These findings suggest that alpha v beta 3 may be a useful therapeutic target for diseases characterized by neovascularization...
  2. ncbi Vertebrate neurogenesis is counteracted by Sox1-3 activity
    Magdalena Bylund
    Ludwig Institute of Cancer Research, Karolinska Institute, Box 240, SE 171 77 Stockholm, Sweden
    Nat Neurosci 6:1162-8. 2003
    ..These data suggest that the generation of neurons from stem cells depends on the inhibition of Sox1-3 expression by proneural proteins...
  3. ncbi Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity
    S Arber
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
    Neuron 23:659-74. 1999
    ..These findings show that HB9 has an essential function in consolidating the identity of postmitotic MNs...
  4. ncbi The avian Z-linked gene DMRT1 is required for male sex determination in the chicken
    Craig A Smith
    Murdoch Children s Research Institute and Department of Paediatrics, The University of Melbourne, Royal Children s Hospital, Melbourne, Victoria 3052, Australia
    Nature 461:267-71. 2009
    ..These results indicate that DMRT1 is required for testis determination in the chicken. Our data support the Z dosage hypothesis for avian sex determination...
  5. ncbi Pax6 is required for the multipotent state of retinal progenitor cells
    T Marquardt
    Max Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Am Fassberg 11, D 37077, Gottingen, Germany
    Cell 105:43-55. 2001
    ....
  6. ncbi Opposing FGF and retinoid pathways control ventral neural pattern, neuronal differentiation, and segmentation during body axis extension
    Ruth Diez del Corral
    Division of Cell and Developmental Biology, Faculty of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
    Neuron 40:65-79. 2003
    ..Our data demonstrate that FGF and RA pathways are mutually inhibitory and suggest that their opposing actions provide a global mechanism that controls differentiation during axis extension...
  7. ncbi Sonic hedgehog mediates the polarizing activity of the ZPA
    R D Riddle
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Cell 75:1401-16. 1993
    ..Like the ZPA, Sonic hedgehog expression leads to the activation of Hox genes. Sonic hedgehog thus appears to function as the signal for antero-posterior patterning in the limb...
  8. ncbi Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein
    A Vortkamp
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 273:613-22. 1996
    ....
  9. ncbi Control of cell behavior during vertebrate development by Slug, a zinc finger gene
    M A Nieto
    Instituto Cajal, Madrid, Spain
    Science 264:835-9. 1994
    ..This change, which is the transition from epithelial to mesenchymal character, occurs at the formation of mesoderm during gastrulation and on emigration of the neutral crest from the neural tube...
  10. ncbi Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10021, USA
    Nat Biotechnol 25:1468-75. 2007
    ..Transplantation of human NCS cells into the developing chick embryo and adult mouse hosts demonstrates survival, migration and differentiation compatible with neural crest ..
  11. ncbi Hedgehog signaling from the ZLI regulates diencephalic regional identity
    Clemens Kiecker
    MRC Centre for Developmental Neurobiology, 4th Floor, New Hunt s House, Guy s Hospital Campus, King s College, London SE1 1UL, UK
    Nat Neurosci 7:1242-9. 2004
    ..We show that, through the release of Shh, the ZLI functions as a local signaling center that regulates the acquisition of identity for these important diencephalic regions...
  12. ncbi Sonic hedgehog regulates the growth and patterning of the cerebellum
    N Dahmane
    The Skirball Institute, Developmental Genetics Program and Department of Cell Biology, NYU School of Medicine, New York, NY 10016, USA
    Development 126:3089-100. 1999
    ..In addition, they provide a cellular context for medulloblastomas, childhood cancers of the cerebellum...
  13. ncbi Directed differentiation of embryonic stem cells into motor neurons
    Hynek Wichterle
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    Cell 110:385-97. 2002
    ..Thus, inductive signals involved in normal pathways of neurogenesis can direct ES cells to form specific classes of CNS neurons...
  14. pmc Characterization of a beta-actin mRNA zipcode-binding protein
    A F Ross
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655, USA
    Mol Cell Biol 17:2158-65. 1997
    ..These data suggest that the 68-kDa ZBP-1 we have isolated and cloned is an RNA-binding protein that functions within a complex to localize beta-actin mRNA...
  15. ncbi Control of planar divisions by the G-protein regulator LGN maintains progenitors in the chick neuroepithelium
    Xavier Morin
    Institute of Developmental Biology of Marseille Luminy, Centre National de la Recherche Scientifique UMR 6216, Case 907, Campus de Luminy, 13288 Marseille Cedex9, France
    Nat Neurosci 10:1440-8. 2007
    ..Gpsm2) in spindle orientation and cell-fate determination in the spinal cord neuroepithelium of the developing chick embryo. We show that LGN is located at the cell cortex and spindle poles of neural progenitors, and that it regulates ..
  16. pmc Innate immune sensing of modified vaccinia virus Ankara (MVA) is mediated by TLR2-TLR6, MDA-5 and the NALP3 inflammasome
    Julie Delaloye
    Department of Medicine, Infectious Diseases Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    PLoS Pathog 5:e1000480. 2009
    ..Delineation of the host response induced by MVA is critical for improving our understanding of poxvirus antiviral escape mechanisms and for designing new MVA vaccine vectors with improved immunogenicity...
  17. pmc Prox1 regulates the notch1-mediated inhibition of neurogenesis
    Valeria Kaltezioti
    Center for Basic Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
    PLoS Biol 8:e1000565. 2010
    ..Together these data suggest that Prox1 inhibits Notch1 gene expression to control the balance between NPC self-renewal and neuronal differentiation...
  18. ncbi Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2
    B G Novitch
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Neuron 31:773-89. 2001
    ..Together, these studies show that Olig2 has a critical role in integrating diverse features of motor neuron differentiation in the developing spinal cord...
  19. ncbi The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells
    Fabrice Lavial
    Institut de Génomique Fonctionnelle de Lyon, Universite de Lyon, Universite Lyon 1, CNRS, INRA, Ecole Normale Superieure de Lyon, France
    Development 134:3549-63. 2007
    ..These findings show that the mechanisms by which Oct4 and Nanog regulate pluripotency and self-renewal are not exclusive to mammals...
  20. ncbi Stem cells, signals and vertebrate body axis extension
    Valerie Wilson
    University of Edinburgh, Edinburgh EH9 3JQ, UK
    Development 136:1591-604. 2009
    ..We also review the location and molecular characteristics of these putative stem cells, along with their evolutionary conservation in vertebrates and the signalling mechanisms that regulate and arrest axis extension...
  21. ncbi Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments
    R Schweitzer
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Development 128:3855-66. 2001
    ..Finally, we show that the endogenous expression of noggin within the condensing digit cartilage contributes to the induction of distal tendons...
  22. ncbi Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb
    B Wang
    Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cell 100:423-34. 2000
    ..The high relative abundance and potency of Gli3 repressor suggest specialization of Gli3 and its products for negative Hedgehog pathway regulation...
  23. ncbi Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication
    Alexander Karlas
    Molecular Biology Department, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
    Nature 463:818-22. 2010
    ..Thus, our results highlight the potency of genome-wide RNAi screening for the dissection of virus-host interactions and the identification of drug targets for a broad range of influenza viruses...
  24. pmc Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes
    Erica Spackman
    Southeast Poultry Research Laboratory, USDA Agricultural Research Service, Athens, Georgia 30605, USA
    J Clin Microbiol 40:3256-60. 2002
    ..The remaining samples were positive with only one detection method. Overall the sensitivity and specificity of the H7- and H5-specific RRT-PCR were similar to those of VI and HI...
  25. ncbi Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling
    J Ericson
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Cell 90:169-80. 1997
    ..2. Pax6 establishes distinct ventral progenitor cell populations and controls the identity of motor neurons and ventral interneurons, mediating graded Shh signaling in the ventral spinal cord and hindbrain...
  26. ncbi Essential role of Gata transcription factors in sympathetic neuron development
    Konstantina Tsarovina
    Max Planck Institut für Hirnforschung, Abteilung Neurochemie, Deutschordenstr 46, 60528 Frankfurt Main, Germany
    Development 131:4775-86. 2004
    ..Gata2 expression is affected in the chick embryo by Bmp gain- and loss-of-function experiments, and by overexpression of Phox2b, Phox2a, Hand2 and Mash1...
  27. ncbi Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol
    A F Moorman
    Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
    J Histochem Cytochem 49:1-8. 2001
    ..The radioactive ISH procedure scarcely permitted detection of these sequences, underscoring the value of this novel method...
  28. pmc Embryonated eggs as an alternative infection model to investigate Aspergillus fumigatus virulence
    Ilse D Jacobsen
    Department for Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany
    Infect Immun 78:2995-3006. 2010
    ..Our results suggest that embryonated eggs can be a very useful alternative infection model to study A. fumigatus virulence and pathogenicity...
  29. ncbi A positive feedback loop coordinates growth and patterning in the vertebrate limb
    L Niswander
    Department of Anatomy, School of Medicine, University of California at San Francisco 94143 0452
    Nature 371:609-12. 1994
    ..Moreover, Shh expression in mesenchyme can be activated by FGF4 in combination with retinoic acid. Once induced, Shh expression can be maintained by FGF4 alone, thus establishing a positive feedback loop between ZPA and ridge...
  30. ncbi The netrins define a family of axon outgrowth-promoting proteins homologous to C. elegans UNC-6
    T Serafini
    Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143 0452
    Cell 78:409-24. 1994
    ..elegans. This homology suggests that growth cones in the vertebrate spinal cord and the nematode are responsive to similar molecular cues...
  31. ncbi Hox repertoires for motor neuron diversity and connectivity gated by a single accessory factor, FoxP1
    Jeremy S Dasen
    Smilow Neuroscience Program, Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY 10016, USA
    Cell 134:304-16. 2008
    ..Our findings show that FoxP1 is a crucial determinant of motor neuron diversification and connectivity, and clarify how this Hox regulatory network controls the formation of a topographic neural map...
  32. ncbi DMRT1 is upregulated in the gonads during female-to-male sex reversal in ZW chicken embryos
    Craig A Smith
    Department of Paediatrics, The University of Melbourne, Royal Children s Hospital, Melbourne, Victoria 3052, Australia
    Biol Reprod 68:560-70. 2003
    ..Higher levels of DMRT1 expression during testis differentiation therefore do not simply reflect a gene dosage difference between the two sexes but imply active involvement in male development...
  33. ncbi Initiation of neural induction by FGF signalling before gastrulation
    A Streit
    Department of Genetics and Development, Columbia University, New York, New York 10032, USA
    Nature 406:74-8. 2000
    ..We show that the organizer and some of its precursor cells produce a fibroblast growth factor signal, which can initiate, and is required for, neural induction...
  34. ncbi Sox10 overexpression induces neural crest-like cells from all dorsoventral levels of the neural tube but inhibits differentiation
    Sonja J McKeown
    Embryology Laboratory, Murdoch Children s Research Institute, Royal Children s Hospital, and Department of Paediatrics, University of Melbourne, Parkville, Victoria, Australia
    Dev Dyn 233:430-44. 2005
    ....
  35. ncbi A requirement for retinoic acid-mediated transcriptional activation in ventral neural patterning and motor neuron specification
    Bennett G Novitch
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Neuron 40:81-95. 2003
    ..Moreover, joint exposure of neural progenitors to retinoids and FGFs suffices to induce motor neuron differentiation in a Shh-independent manner...
  36. ncbi Differential activities of Sonic hedgehog mediated by Gli transcription factors define distinct neuronal subtypes in the dorsal thalamus
    Kazue Hashimoto-Torii
    Division of Morphogenesis, Department of Embryogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2 2 1, Kumamoto 860 0811, Japan
    Mech Dev 120:1097-111. 2003
    ..These results suggest that cooperation of the differential expression of Gli factors and the activity gradient of Shh signaling generates the distinct thalamic neurons at the specific locations...
  37. pmc Transcriptional control of axonal guidance and sorting in dorsal interneurons by the Lim-HD proteins Lhx9 and Lhx1
    Oshri Avraham
    Department of Medical Neurobiology, IMRIC, Hebrew University, Hadassah Medical School, Jerusalem, Israel
    Neural Dev 4:21. 2009
    ..While they are not required for cell fate acquisition, their role in patterning the axonal trajectory of dI1 and dI2 neurons remains incompletely understood...
  38. ncbi Role of fusion protein cleavage site in the virulence of Newcastle disease virus
    Aruna Panda
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA
    Microb Pathog 36:1-10. 2004
    ....
  39. ncbi Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific expression of novel cadherins
    S Nakagawa
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Development 121:1321-32. 1995
    ..The cadherin-mediated specific interaction between crest cells likely plays a role in intercellular signaling between homotypic cells as well as in sorting of heterotypic cells...
  40. ncbi Sox21 promotes the progression of vertebrate neurogenesis
    Magnus Sandberg
    Ludwig Institute for Cancer Research, Karolinska Institute, Box 240, SE 171 77, Stockholm, Sweden
    Nat Neurosci 8:995-1001. 2005
    ..Using genetic studies in the chick embryo, we found that Sox21 mediates this function by counteracting the activity of Sox1-3...
  41. ncbi Timing and kinetics of E- to N-cadherin switch during neurulation in the avian embryo
    Alwyn Dady
    Universite Pierre et Marie Curie Paris 6, Laboratoire de Biologie du Developpement, Paris, France
    Dev Dyn 241:1333-49. 2012
    ..An E- to N-cadherin switch also occurs during neurogenesis, but this is not accompanied with EMT. The biological significance of this switch is currently unknown...
  42. pmc Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence
    B P Peeters
    Department of Avian Virology, Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands
    J Virol 73:5001-9. 1999
    ..These results demonstrate that genetically modified NDV can be recovered from cloned cDNA and confirm the supposition that cleavage of the F0 protein is a key determinant in virulence of NDV...
  43. ncbi Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c expression by FGFs, Gdf11, and retinoids
    J P Liu
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Neuron 32:997-1012. 2001
    ....
  44. ncbi A hedgehog-insensitive form of patched provides evidence for direct long-range morphogen activity of sonic hedgehog in the neural tube
    J Briscoe
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Institute, Columbia University, 701 West 168th Street, 10032, New York, NY, USA
    Mol Cell 7:1279-91. 2001
    ..mPtc1(Deltaloop2) expression also caused the abnormal spread of Shh to more dorsal cells, indicating that Shh in the neural tube, like Hh in Drosophila, induces a feedback mechanism that limits its range of action...
  45. pmc The inhibition of tumor cell intravasation and subsequent metastasis via regulation of in vivo tumor cell motility by the tetraspanin CD151
    Andries Zijlstra
    Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cancer Cell 13:221-34. 2008
    ....
  46. pmc Efficient production of germline transgenic chickens using lentiviral vectors
    Michael J McGrew
    Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    EMBO Rep 5:728-33. 2004
    ....
  47. ncbi Neural crest emigration from the neural tube depends on regulated cadherin expression
    S Nakagawa
    Department of Biophysics, Faculty of Science, Kyoto University, Kitashirakawa, Sakyo ku, Kyoto 606 8502, Japan
    Development 125:2963-71. 1998
    ..These findings suggest that the regulation of cadherin expression or its activity at the neural crest-forming area plays a critical role in neural crest emigration from the neural tube...
  48. ncbi Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector
    Makoto Motono
    Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8603, Japan
    J Biosci Bioeng 109:315-21. 2010
    ..In G(1) transgenic chickens obtained by this method, enhanced green fluorescent protein was effectively expressed under the control of the actin promoter...
  49. pmc Pathogenesis of Candida albicans infections in the alternative chorio-allantoic membrane chicken embryo model resembles systemic murine infections
    Ilse D Jacobsen
    Department for Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany
    PLoS ONE 6:e19741. 2011
    ..Despite its limitations, it presents a useful alternative tool to pre-screen C. albicans strains to select strains for subsequent testing in murine models...
  50. pmc Bmp4 is essential for the formation of the vestibular apparatus that detects angular head movements
    Weise Chang
    National Institute on Deafness and Other Communication Disorders, NIH, Rockville, Maryland, United States of America
    PLoS Genet 4:e1000050. 2008
    ..Our results suggest that, in comparison to sensory bristles, crista formation within the inner ear requires an additional step of sensory and non-sensory fate specification...
  51. ncbi A modified cornish pasty method for ex ovo culture of the chick embryo
    Hiroki Nagai
    Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Japan
    Genesis 49:46-52. 2011
    ..Combination of the MC culture with electroporation and labeling techniques will be a valuable new tool in cell lineage tracing and molecular functional analyses of early avian embryogenesis...
  52. pmc Early development of the central and peripheral nervous systems is coordinated by Wnt and BMP signals
    Cedric Patthey
    Umeå Center for Molecular Medicine, Umea University, Umea, Sweden
    PLoS ONE 3:e1625. 2008
    ....
  53. ncbi Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo
    Andrea Wizenmann
    Institute of Developmental Genetics, Helmholtz Zentrum Munich, Neuherberg, Germany
    Neuron 64:355-66. 2009
    ..Collectively, our results indicate that extracellular Engrailed contributes to retinotectal mapping in vivo by modulating the sensitivity of growth cones to EphrinA...
  54. ncbi Eight-plasmid system for rapid generation of influenza virus vaccines
    Erich Hoffmann
    Department of Virology and Molecular Biology, St Jude Children s Research Hospital, 332 North Lauderdale, Memphis, TN 38105 2794, USA
    Vaccine 20:3165-70. 2002
    ..Our findings demonstrate that the eight-plasmid system allows the rapid and reproducible generation of reassortant influenza A viruses for use in the manufacture of vaccines...
  55. ncbi WNT11 acts as a directional cue to organize the elongation of early muscle fibres
    Jerome Gros
    Developmental Biology Institute of Marseille Luminy IBDML, Universite de la Mediterranee, CNRS UMR 6216, Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France
    Nature 457:589-93. 2009
    ..This study also provides evidence that WNTs can act as instructive cues to regulate the PCP pathway in vertebrates...
  56. ncbi Early regionalization of the otic placode and its regulation by the Notch signaling pathway
    Gina Abello
    DCEXS Universitat Pompeu Fabra, C Dr Aiguader 88, 08003 Barcelona, Spain
    Mech Dev 124:631-45. 2007
    ....
  57. pmc Development of a real-time reverse-transcription PCR for detection of newcastle disease virus RNA in clinical samples
    Mark G Wise
    Southeast Poultry Research Laboratory, Agricultural Research Service, US Department of Agriculture, Athens, Georgia 30605, USA
    J Clin Microbiol 42:329-38. 2004
    ..Overall, a positive correlation was obtained between the RRT-PCR results and virus isolation for NDV from clinical samples...
  58. pmc Neural crest origin of olfactory ensheathing glia
    Perrine Barraud
    Department of Physiology, University of Cambridge, Cambridge CB2 3DY, United Kingdom
    Proc Natl Acad Sci U S A 107:21040-5. 2010
    ....
  59. ncbi Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1
    J Ericson
    Department of Microbiology, Umea University, Sweden
    Science 256:1555-60. 1992
    ..These results establish Islet-1 as the earliest marker of developing motor neurons. The molecular nature of the Islet-1 protein suggests that it may be involved in the establishment of motor neuron fate...
  60. ncbi Synchronization of neurogenesis and motor neuron specification by direct coupling of bHLH and homeodomain transcription factors
    Soo Kyung Lee
    Gene Expression Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Neuron 38:731-45. 2003
    ..This functional link acts to synchronize neuronal subtype specification with neurogenesis...
  61. ncbi Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif
    Diogo S Castro
    Division of Molecular Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Dev Cell 11:831-44. 2006
    ..We thus propose that Mash1 synergizes with Brn factors to regulate multiple steps of neurogenesis...
  62. ncbi Neural induction: old problem, new findings, yet more questions
    Claudio D Stern
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 132:2007-21. 2005
    ..This review summarizes neural induction events in different species and highlights some unanswered questions about this important developmental process...
  63. ncbi Ets-1 expression is associated with cranial neural crest migration and vasculogenesis in the chick embryo
    Siroun A Tahtakran
    Department of Cell and Neurobiology, University of Southern California, Keck School of Medicine, 1333 San Pablo Street, BMT 401, Los Angeles, CA 90089, USA
    Gene Expr Patterns 3:455-8. 2003
    ..In addition to neural crest, we find expression of c-Ets-1 in endothelial cells of blood vessels, in somitic and intermediate mesoderm, in limb buds and in the heart...
  64. ncbi The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials
    T I Valdes
    Center for Biomaterials, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030 1615, USA
    J Biomed Mater Res 62:273-82. 2002
    ....
  65. ncbi Induction of the neural crest and the opportunities of life on the edge
    Xiao Huang
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Biol 275:1-11. 2004
    ..We also summarize some of these findings and discuss how the differential activation of these genes may contribute to the establishment of neural crest diversity...
  66. ncbi Wnt2b controls retinal cell differentiation at the ciliary marginal zone
    Fumi Kubo
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake cho, Kyoto 606 8502, Japan
    Development 130:587-98. 2003
    ..These results suggest that Wnt2b functions to maintain undifferentiated progenitor cells in the ciliary marginal zone, and thus serves as a putative stem cell factor in the retina...
  67. ncbi The snail superfamily of zinc-finger transcription factors
    M Angela Nieto
    Instituto Cajal, Doctor Arce, 37, 28002 Madrid, Spain
    Nat Rev Mol Cell Biol 3:155-66. 2002
    ..Different family members have also been implicated in the signalling cascade that confers left right identity, as well as in the formation of appendages, neural differentiation, cell division and cell survival...
  68. ncbi Control of cell pattern in the neural tube: regulation of cell differentiation by dorsalin-1, a novel TGF beta family member
    K Basler
    Howard Hughes Medical Institute, Columbia University, New York, New York 10032
    Cell 73:687-702. 1993
    ..These findings suggest that dsl-1 regulates the differentiation of cell types along the dorsoventral axis of the neural tube, acting in conjunction with distinct ventralizing signals from the notochord and floor plate...
  69. ncbi Thalamic development induced by Shh in the chick embryo
    Claudia Vieira
    Neuroscience Institute, Miguel Hernandez University N 332, Km 87, E 03550 Alicante, Spain
    Dev Biol 284:351-63. 2005
    ..Overall, these results indicate that Shh expressed in the ZLI plays an important role in diencephalic growth and in the development of the thalamus...
  70. ncbi Integration between the epibranchial placodes and the hindbrain
    J Begbie
    Medical Research Council (MRC) Centre for Developmental Neurobiology, Fourth Floor, New Hunts House, Guys Campus, Kings College London, London SE1 9RT, UK
    Science 294:595-8. 2001
    ..This work defines a role for the neuroglial hindbrain crest in organizing the afferent innervation of the hindbrain...
  71. pmc The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism and virulence
    Zhuhui Huang
    Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA
    J Virol 78:4176-84. 2004
    ..These results are consistent with the hypothesis that the virulence of NDV is multigenic and that the cleavability of F protein alone does not determine the virulence of a strain...
  72. ncbi Segmentation in the chick embryo hindbrain is defined by cell lineage restrictions
    S Fraser
    Department of Physiology and Biophysics, University of California, Irvine 92717
    Nature 344:431-5. 1990
    In the chick embryo hindbrain, morphological segmentation into rhombomeres is matched by metameric patterns of early neuronal differentiation and axonogenesis...
  73. ncbi A spatial and temporal map of FGF/Erk1/2 activity and response repertoires in the early chick embryo
    J Simon Lunn
    Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow St, Dundee DD1 5EH, UK
    Dev Biol 302:536-52. 2007
    ..To begin to assess specificity downstream of FGF in the chick embryo we have characterised the patterns of Fgfr1-4 expression and Erk1/2 activation, as well as expression of the ..
  74. ncbi Evolution of the turtle body plan by the folding and creation of new muscle connections
    Hiroshi Nagashima
    Laboratory for Evolutionary Morphology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minami, Kobe 650 0047, Japan
    Science 325:193-6. 2009
    ..We propose that the evolutionary origin of the turtle body plan results from heterotopy based on folding and novel connectivities...
  75. ncbi Improved method for chick whole-embryo culture using a filter paper carrier
    S C Chapman
    MRC Centre for Developmental Neurobiology, King s College London, Guy s Hospital, London, United Kingdom
    Dev Dyn 220:284-9. 2001
    ..We also discuss some alternative methods for setting up these cultures...
  76. ncbi Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells
    N Tsunekawa
    Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194 8511, Japan
    Development 127:2741-50. 2000
    ..These results strongly suggest that the chicken germline is determined by maternally inherited factors in the germ plasm...
  77. ncbi The transcriptional control of trunk neural crest induction, survival, and delamination
    Martin Cheung
    Developmental Neurobiology, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK
    Dev Cell 8:179-92. 2005
    ..Together, the data suggest a model in which a combination of transcription factors regulates the acquisition of the diverse properties of neural crest cells...
  78. ncbi Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton
    C Hartmann
    Department of Genetics, Harvard Medical School, 02115, Boston, MA, USA
    Cell 104:341-51. 2001
    ..Induction of an ectopic joint-like region by Wnt-14 suppresses the formation of the immediately adjacent endogenous joint, potentially providing insight into the spacing of joints...
  79. ncbi Regulation of motor neuron pool sorting by differential expression of type II cadherins
    Stephen R Price
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Cell 109:205-16. 2002
    ..Type II cadherins are also expressed by proprioceptive sensory neurons, raising the possibility that cadherins regulate additional steps in the development of sensory-motor circuits...
  80. ncbi Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility
    H L Zhang
    Department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuron 31:261-75. 2001
    ..Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA...
  81. ncbi Ectodermal Wnt function as a neural crest inducer
    MARTIN I GARCIA-CASTRO
    Division of Biology 139 74, California Institute of Technology, Wilson and California, Pasadena, CA 91125, USA
    Science 297:848-51. 2002
    ..Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos...
  82. pmc A functional study of miR-124 in the developing neural tube
    Xinwei Cao
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Genes Dev 21:531-6. 2007
    ..Thus miR-124 appears to ensure that progenitor genes are post-transcriptionally inhibited in neurons...
  83. ncbi Molecular interactions coordinating the development of the forebrain and face
    Ralph S Marcucio
    Department of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 257 Campus Drive, Stanford, CA 94305, USA
    Dev Biol 284:48-61. 2005
    ....
  84. ncbi Wnt signaling is required at distinct stages of development for the induction of the posterior forebrain
    Michelle M Braun
    Program in Neurobiology and Behavior, Department of Biological Structure, Box 357420, University of Washington, Seattle, WA 98195, USA
    Development 130:5579-87. 2003
    ..Later, continued Wnt signaling resulted in the induction of dT specific markers, but only in tissues that expressed Irx3...
  85. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
    ..Thus, the techniques proposed in this study provide a novel approach to study the mechanisms of late organogenesis, for which chickens are most suitable model animals...
  86. ncbi FGF signaling controls somite boundary position and regulates segmentation clock control of spatiotemporal Hox gene activation
    J Dubrulle
    , Developmental Biology Institute of Marseille (IBDM, , Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France
    Cell 106:219-32. 2001
    ..Furthermore, by manipulating boundary position in the chick embryo, we show that Hox gene expression is maintained in the appropriately numbered somite rather than at an absolute ..
  87. ncbi A balance of FGF, BMP and WNT signalling positions the future placode territory in the head
    Anna Litsiou
    Department of Craniofacial Development, Guys Campus, Guys Tower, Floor 27, King s College London, London SE1 9RT, UK
    Development 132:4051-62. 2005
    ..This mechanism explains how the placode territory becomes confined to the head, and how neural crest and placode fates diversify...
  88. pmc Morphogenesis of a highly replicative EGFPVP22 recombinant Marek's disease virus in cell culture
    C Denesvre
    Laboratoire Virologie Moléculaire, INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, France
    J Virol 81:12348-59. 2007
    ..The discrepancy between the efficiency with which this MDV mutant spreads in cell culture and the relatively inefficient process of its envelopment and virion release raises the question of the MDV cell-to-cell spreading mechanism...
  89. ncbi Mechanistic differences in the transcriptional interpretation of local and long-range Shh morphogen signaling
    Tony Oosterveen
    Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden
    Dev Cell 23:1006-19. 2012
    ....
  90. ncbi Expression of the retinoic acid catabolising enzyme CYP26B1 in the chick embryo and its regulation by retinoic acid
    Susan Reijntjes
    MRC Centre for Developmental Neurobiology, 4th Floor, New Hunt s House, King s College London, Guy s Campus, London Bridge, SE1 1UL, London, UK
    Gene Expr Patterns 3:621-7. 2003
    ..In the absence of RA, Cyp26B1 is only expressed in the hindbrain and fails to be expressed in all the other regions of the embryo, most dramatically in the trunk. Adding back RA rescues Cyp26B1 expression...
  91. pmc Introduction of the six major genomic deletions of modified vaccinia virus Ankara (MVA) into the parental vaccinia virus is not sufficient to reproduce an MVA-like phenotype in cell culture and in mice
    Christine Meisinger-Henschel
    Bavarian Nordic GmbH, Fraunhoferstrasse 13, 82152 Martinsried, Germany
    J Virol 84:9907-19. 2010
    ..Host range restriction and avirulence of MVA are most likely a cooperative effect of gene deletions and mutations involving the major deletions...
  92. ncbi Easy and efficient protocols for working with recombinant vaccinia virus MVA
    Melanie Kremer
    Institute for Infectious Diseases and Zoonoses, University of Munich LMU, Munich, Germany
    Methods Mol Biol 890:59-92. 2012
    ..Importantly, we include methodology for rigid quality control to obtain best possible vector viruses for further investigations including clinical evaluation...
  93. pmc Induction of Noxa-mediated apoptosis by modified vaccinia virus Ankara depends on viral recognition by cytosolic helicases, leading to IRF-3/IFN-β-dependent induction of pro-apoptotic Noxa
    Pedro Eitz Ferrer
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
    PLoS Pathog 7:e1002083. 2011
    ..We thus here describe a pathway leading from the detection of viral RNA during MVA infection by the cytosolic helicase-pathway, to the up-regulation of Noxa and apoptosis via IRF3 and type I IFN signalling...
  94. pmc BMP signaling mediates stem/progenitor cell-induced retina regeneration
    Tracy Haynes
    Department of Zoology, Miami University, Oxford, OH 45056, USA
    Proc Natl Acad Sci U S A 104:20380-5. 2007
    ..Retina stem/progenitor cells exist in other species, including humans. Thus, our findings provide insights on how retinal stem cells can be activated for possible regenerative therapies...
  95. ncbi Early subdivisions in the neural plate define distinct competence for inductive signals
    Daisuke Kobayashi
    Department of Neurobiology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Development 129:83-93. 2002
    ..These findings provide evidence that the early embryonic forebrain is demarcated into two domains with distinct genetic programs, which argues against the authentic telendiencephalic subdivision...
  96. pmc Early acquisition of neural crest competence during hESCs neuralization
    Carol Lynn Curchoe
    Sanford Burnham Institute for Medical Research, La Jolla, California, United States of America
    PLoS ONE 5:e13890. 2010
    ....
  97. ncbi Gain- and loss-of-function approaches in the chick embryo
    Tatjana Sauka-Spengler
    California Institute of Technology, Pasadena, California 91125, USA
    Methods Cell Biol 87:237-56. 2008
    ..methods for rapid, transient spatiotemporally targeted gene alterations have thus again re-established the chick embryo as an important experimental niche by making it possible to apply genetics in concert with classical ..
  98. pmc Apical accumulation of Rho in the neural plate is important for neural plate cell shape change and neural tube formation
    Nagatoki Kinoshita
    Electron Microscope Laboratory and Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650 0047, Japan
    Mol Biol Cell 19:2289-99. 2008
    ....
  99. ncbi Experimental model and immunohistochemical comparison of U87 human glioblastoma cell xenografts on the chicken chorioallantoic membrane and in rat brains
    Tadej Strojnik
    Department of Neurosurgery, University Clinical Centre Maribor, Ljubljanska 5, SI 2000 Maribor, Slovenia
    Anticancer Res 30:4851-60. 2010
    ..The immunohistochemical characteristics were studied and compared with an orthotopic rodent model...
  100. ncbi Sharpening of the anterior neural border in the chick by rostral endoderm signalling
    Luisa Sánchez-Arrones
    Department of Human Anatomy and Psychobiology, University of Murcia, School of Medicine, Murcia, Spain
    Development 139:1034-44. 2012
    ..We also show that concurrent signals from the endodermal layer are necessary to position and sharpen the neural border, and suggest that FGF8 might be a component of this signalling...
  101. ncbi Alternative splicing of the Robo3 axon guidance receptor governs the midline switch from attraction to repulsion
    Zhe Chen
    Division of Research, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA
    Neuron 58:325-32. 2008
    ..The tight spatial regulation of opponent splice variants helps ensure high-fidelity transition of axonal responses from attraction to repulsion at the midline...

Research Grants63

  1. CONTROL OF MUSCLE PROTEIN SYNTHESIS DURING MYOGENESIS
    Charles P Emerson; Fiscal Year: 2013
    ..mESC) model will be used in combination with expression, shRNA knockdown, array profiling, cell culture, and chick embryo and muscle engraftment approaches to identify genes and signals that control the specifications of mesoderm and ..
  2. Effects of Hypoxia on Craniofacial Morphology and Molecular Signaling
    FRANCIS JOEL SMITH; Fiscal Year: 2011
    ..understand the mechanisms underlying craniofacial malformations induced by hypoxia, I have designed a hypoxic chick embryo model by decreasing the level of oxygen in ovo...
  3. Human Myocardium Engineered Using Developmentally-Inspired Protein Scaffolds
    ADAM WALTER FEINBERG; Fiscal Year: 2012
    ..We will use the chick embryo as an experimentally tractable model system...
  4. Ephrin-A1 in Tumor-Endothelial Interaction During Metastasis
    Jin Chen; Fiscal Year: 2012
    ..Two novel model systems, the chick embryo metastasis model and the tumor-microenvironment bioreactor, will be used for visualization and quantification ..
  5. Retinal Pigmented Epithelium Reprogramming and Retina Regeneration
    Katia Del Rio-Tsonis; Fiscal Year: 2013
    ..to explore possible therapies, it is important to use animal models with regenerative capabilities such as the chick embryo. Embryonic chicks regenerate their retina, following retinectomy, via the process of trans-differentiation if ..
  6. Osteoprotegerin in breast cancer cells: role in tumor growth and metastasis
    Linda Connelly; Fiscal Year: 2012
    ..These cells will then be used to investigate the consequences of OPG knockdown on metastasis in the chick embryo metastasis model...
  7. A Cellular and Molecular Analysis of the Intravasation Step in Tumor Metastasis
    James P Quigley; Fiscal Year: 2012
    ..These two tumor dissemination variants, HT- hi/diss and HT-lo/diss, were selected and monitored in the chick embryo model, where primary human tumors developing on the embryo's chorioallantoic membrane (CAM), recapitulate ..
  8. FGF-P to treat post-irradiation GI syndrome
    Aiguo Zhang; Fiscal Year: 2013
    ..Sub-aims include: (2.1) Chick embryo angiogenesis assays;(2...
  9. The role of Nectins in fusion of the midface and genesis of cleft lip and palate.
    TIMOTHY CHILTON COX; Fiscal Year: 2012
    ..family of cell adhesion proteins, the nectins, in the normal process of lip and palate formation using the chick embryo as the model system...
  10. FORMING AND PATTERNING THE VERTEBRATE INNER EAR
    GARY SCHOENWOLF; Fiscal Year: 2009
    ..project period in formation of the otic placode, are localized in the cephalic paraxial mesoderm of the early chick embryo. This will allow us to determine how the rudiments of the inner ear are positioned within the head of the ..
  11. Genetic regulation of Inner and Middle Ear Development
    Andrew K Groves; Fiscal Year: 2013
    ..will determine the function and mechanism of Foxi3 during development of the inner ear using knockout mice, chick embryo manipulations and state-of-the-art ES cell models, deep sequencing and bioinformatic analysis...
  12. Profiling Early Metastatic Melanoma in vivo using the Chick Embryo Model
    CALEB MICHAEL BAILEY; Fiscal Year: 2012
    ..abstract_text> ..
  13. CRANIOFACIAL MORPHOGENESIS IN PRENATAL ALCOHOL EXPOSURE
    Susan M Smith; Fiscal Year: 2013
    ..This work is a logical extension ofthe current award. We continue using our established chick embryo model, which replicates the alcohol responses of mammals including humans, has well-described NC development, ..
  14. Transmembrane Proteins Involved in Human Tumor Expansion
    James P Quigley; Fiscal Year: 2013
    ..Panels of generated mAbs are screened for their ability to inhibit human tumor dissemination in chick embryo and mouse models of metastasis. Using this approach, we have generated unique antibodies...
  15. TRANSCRIPTIONAL CONTROL OF MAMMALIAN BRAIN DEVELOPMENT
    Eseng Lai; Fiscal Year: 2001
    ..domains of BF-1 in terms of its ability to antagonize cell cycle arrest mediated by TGF-b and to transform chick embryo fibroblasts, since a chick homolog of BF-1 has been shown by others to be a proto-oncoprotein 2) to examine the ..
  16. Project 1: Immunogens and Manufacturing
    Harriet L Robinson; Fiscal Year: 2012
    ..Production of MVA vaccines is a challenge because of the growth of these vaccines in chick embryo fibroblasts that require sourcing and producing large batches of primary cells...
  17. Genes regulating neurogenesis in the trigeminal placode
    Michael R Stark; Fiscal Year: 2010
    ..development and testing of misexpression and inhibitory constructs for these genes, which will be used in the chick embryo model system to fulfill the specific objectives...
  18. Total Synthesis of Bioactive Marine Natural Products
    Armen Zakarian; Fiscal Year: 2013
    ..will investigate in vivo biodistribution of these marine toxins and their effect of developmental biology using chick embryo as a model...
  19. BIOLOGY AND PATHOLOGY OF A MODULATOR OF FGF
    Anton Wellstein; Fiscal Year: 2010
    ..Aim 3. To study the impact of deletion of selected BPs. Chick embryo and mouse models will be employed in developmental and tumor biology studies.
  20. Development and testing of a law for morphomechanics at multiple scales
    Matthew A Wyczalkowski; Fiscal Year: 2012
    ..Test morphomechanical laws in tissue wounding experiments. Small circular incisions will be made in unexcised chick embryo epithelia and the rate of wound closure quantified...
  21. Expression and regulation of the axon guidance cues
    Zheng Zheng Bao; Fiscal Year: 2009
    ..regulation and interplay of multiple axon guidance cues in retinal axon guidance, by combining mouse and chick embryo model systems and employing various in vivo and in vitro assays...
  22. Craniofacial defects in ethanol-exposed zebrafish
    Sara Ahlgren; Fiscal Year: 2004
    ..Partial inhibition of Sonic hedgehog in the chick embryo using function-blocking antibodies results in a phenotype similar to a mild holoprosencephaly that is ..
  23. Microcarrier / CEF / Media SYSTEM to make Viral Vaccines
    WILLIAM HILLEGAS; Fiscal Year: 2002
    ..Primary chick embryo fibroblast (CEF) cells are widely used in viral vaccine production (for both avian and human vaccines), but ..
  24. HOST CELL INTERACTION OF ANIMAL VIRUSES
    Philip Marcus; Fiscal Year: 1990
    ..interferon, (6) To study a double-stranded RNA inducible double-stranded ribonuclease discovered in aged chick embryo cells, (7) To genetically clone 3'-transcribing defective-interfering particles of vesicular stomatitis virus ..
  25. REGULATION OF NA,K ATPASE BY THE AH RECEPTOR
    Mary Walker; Fiscal Year: 2004
    ..In the chick embryo, TCDD reduces myocardial Na+/K+ ATPase alpha1 protein expression, induces a dilated cardiomyopathy, and alters ..
  26. A MICROCARRIER/CEF/MEDIA SYSTEM TO MAKE VIRAL VACCINES
    WILLIAM HILLEGAS; Fiscal Year: 2000
    The research proposed is aimed at developing a system for growing chick embryo fibroblast (CEF) cells on microcarriers in bioreactors for the large- scale production of avian and human virus vaccines...
  27. IMMUNOCYTOCHEMICAL STUDY OF CARDIAC MUSCLE ORGANIZATION
    KIYOTERU TOKUYASU; Fiscal Year: 1990
    ..is a fundamental question in understanding the developmental process of cardiac muscle organization, in the chick embryo heart, myofibrils first appear at H-H stage 10 (1...
  28. GUIDANCE OF MOTONEURON GROWTH CONES
    Kathryn Tosney; Fiscal Year: 1991
    ..and descriptive studies of a simple vertebrate system, the development of muscle innervation in the chick embryo, have shown that selective pathfinding by the growing of individual neurites (the "growth cones") plays an ..
  29. B-CLASS EPHRINS IN AXON GUIDANCE
    Andrew Bergemann; Fiscal Year: 2004
    ..Ephrin B2 undergoes proteolysis in chick embryo fibroblasts. The proteolysis appears to be dependent upon clustering...
  30. Chemopreventive effects of dietary cottonseed oil
    YOUNG LIN; Fiscal Year: 2003
    ..is associated with elevated TGF-B; (c) GP inhibited the in vitro invasive activity of MAT-LyLu cells; (d) the chick embryo chorioallantoic membrane assay suggested that GP inhibits angiogenesis; (e) a novel MAT-LyLu subline has been ..
  31. Global Patterning in Vertebrate Mesoderm
    Ann Burke; Fiscal Year: 2005
    ..Our work to date has identified two dynamic domains in the developing chick embryo (Figure 3). The primaxial domain is populated only by cells from the somites...
  32. MOLECULAR CHARACTERIZATION OF A NEURAL PROTEIN
    SUSANNAH CHANG; Fiscal Year: 1993
    ..DM-RASP is expressed on the surface of some but not all early axons in the developing chick embryo. Thus it is possible that DM-RASP may be involved in helping axons make pathway specific choices...
  33. EMBRYOS FOR DETECTING ENVIRONMENTAL MUTAGENS
    Stephen Bloom; Fiscal Year: 1992
    ..We propose to use the chick embryo to study the nature of mutagen-induced SCE and the effects of such in the hemopoietic and immune systems at pre-..
  34. CONTROL OF CELL GROWTH BY MITOGEN RECEPTORS
    JAMES PERDUE; Fiscal Year: 1980
    ..in vivo replication by characterizing insulin-like activity (ILAS) and insulin binding to substratum-attached chick embryo fibroblasts (CEF), RSV-transformed CEF, and to isolated plasma membrane from these cells. ILAs with a pI of 6...
  35. REGULATION OF PURKINJE FIBER DEVELOPMENT IN THE HEART
    Takashi Mikawa; Fiscal Year: 2005
    ..Our retroviral cell lineage studies of the chick embryo have demonstrated conclusively that impulse-conducting cells differentiate from working myocytes and are ..
  36. AI ADENOSINE RECEPTOR IN CULTURED HEART CELLS
    Bruce Liang; Fiscal Year: 1993
    ..Monolayer atrial cells cultured from 14 day chick embryo will be used as a model system...
  37. Origin and Fate of Myogenic Stem Cells
    Mindy George Weinstein; Fiscal Year: 2006
    ..are that stem cells committed to the skeletal muscle lineage: (1) are present in the epiblast of the early chick embryo, (2) become incorporated into organs lacking skeletal muscle during gastrulation, and (3) are able to induce ..
  38. Molecular Pathway in Retina Regeneration
    Katia Del Rio Tsonis; Fiscal Year: 2004
    In the chick embryo, retinal pigment epithelial cells (rPEC) transdifferentiate to neural retina cells under the influence of Fibroblast Growth Factor-2 (FGF-2)...
  39. MATRIX METALLOPROTEASES IN AVIAN AND HUMAN TUMOR CELLS
    James Quigley; Fiscal Year: 2009
    ..Our laboratory has utilized an alternative in vivo tumor progression model, namely a human tumor/chick embryo system that is both rapid and quantitative...
  40. EMBRYONIC BONE FORMATION DURING ALUMINUM TOXICITY
    CONRAD FIRLING; Fiscal Year: 1990
    ..This research will utilize long bone development in the chick embryo as an experimental model...
  41. MICROVASCULAR CONTROL--VASCULARITY AND FLUID ENVIRONMENT
    THOMAS ADAIR; Fiscal Year: 1992
    ..I have developed a whole body perfusion technique in the chick embryo which makes it possible to estimate vascularity rapidly...
  42. NUCLEAR MAGNETIC RESONANCE AND EMBRYONIC DEVELOPMENT
    JOSEPH YIP; Fiscal Year: 1992
    ..Crucial to any scientific study is the experimental model system. In this study, the chick embryo was chosen for obvious advantages...
  43. Analyses of stem cells in the chick Embryo and Fetus
    Mindy George Weinstein; Fiscal Year: 2001
    ..Furthermore, the origin of adult stem cells is unknown. We propose to use the chick embryo as a model system with which to study these basic questions in stem cell biology...
  44. MUSCARINIC CHOLINERGIC RECEPTORS IN THE HEART
    NEIL NATHANSON; Fiscal Year: 2000
    ..The chick embryo represents an attractive system for the study of cardiac mAChR both in vivo and in cell culture...
  45. TREATMENT OF OVARIAN CANCER WITH CONTORTROSTATIN
    FRANCIS MARKLAND; Fiscal Year: 2001
    ..Using the chick embryo chorioallantoic membrane (CAM) model system, CN blocked tumor cell dissemination and inhibited angiogenesis ..
  46. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    JAMIL SCOTT; Fiscal Year: 2006
    The specific aims of this research proposal are to identify stem cells in the chick embryo that can give rise to kidney tissue, and to characterize the mechanism by which this differentiation to kidney occurs, using both kidney and non-..
  47. EMBRYONIC CELL ASSOCIATION & DEVELOPMENT
    David Yang; Fiscal Year: 1980
    ..We have succeeded in isolating three different types of cell surface proteins from chick embryo retina, cerebrum, cerebellum, kidney, heart and lung...
  48. The Specification of the Hem. and Cardiac Lineages
    Gordon Keller; Fiscal Year: 2006
    ..molecules in these process were identified through the use of model systems such as Xenopus, Zebrafish and the chick embryo that provide access to cell population undergoing mesoderm commitment...
  49. DEVELOPMENT OF CARDIAC EXCITATION/CONTRACTION COUPLING
    Tony Creazzo; Fiscal Year: 2002
    ..complexes are the structural manifestations of EC coupling which have recently been reported in detail in the chick embryo with electron and confocal microscopy...
  50. COMMITMENT AND DEVELOPMENT OF SOMITIC MYOBLASTS
    Mindy George Weinstein; Fiscal Year: 1990
    ..and somites and to follow their pathways of migration and maturational changes during the development of the chick embryo. The antibodies also will be used to isolate and analyze cell surface molecules by immunoprecipitation, ..
  51. Cellular and Molecular Basis for TRI Cardiotoxicity
    John Lough; Fiscal Year: 2005
    ..This proposal combines in vitro and in ovo experiments using the chick embryo model with in vivo experiments using the mouse model to test the hypothesis that TRI is a cardiac teratogen ..
  52. PATTERNING THE DEVELOPING BRAIN
    Jeffrey Ming; Fiscal Year: 2002
    ..of BMP5 action in the forebrain will be examined by studying the function of BMP receptors (BMPRs) in the chick embryo, an established model of brain development...
  53. Function of CMF1 Protein During Avian Myogenesis
    MARY DEES; Fiscal Year: 2006
    ..CMF1 is unique in its restriction to developing cardiac and skeletal muscle in the developing chick embryo. Its expression precedes that of contractile proteins in the developing heart tube and in skeletal myoblasts in ..
  54. DETERMINATION OF EMBRYONIC POLARITY
    VIRGINIA PAPAIOANNOU; Fiscal Year: 2000
    ..the mechanisms that determine embryonic polarity in the early stages of development will be studied using the chick embryo as a model, because of their ease of manipulation and culture...
  55. INTERACTIVE 3D MODELS OF CARDIAC MORPHOGENESIS
    MICHAEL HULTNER; Fiscal Year: 1999
    ..II of this study, we propose to use the techniques pioneered during Phase I, along with student feedback on the chick embryo models, to develop similar, yet more sophisticated models that show the full range of human cardiac development...
  56. MICROCIRCULATORY ADAPTATIONS AND THEIR CONTROL
    THOMAS ADAIR; Fiscal Year: 1993
    ..The chick embryo is used in many experiments because its cardiovascular system grows rapidly making possible to study- within ..
  57. Communication among Migrating Neurons in Auditory Anlage
    Susan Hendricks; Fiscal Year: 2004
    ..NM and NL cells are generated from distinct rhombomeric regions in the chick embryo. During development, these cells coalesce within the auditory anlage prior to migrating to their mature ..
  58. Neurogenesis in the Chick Embryo Spinal Cord
    MARGARET HOLLYDAY; Fiscal Year: 2004
    ..Deficiencies in neurogenesis may be underlying causes of human congenital brain abnormalities and/or mental retardation. ..
  59. PROTEASES IN GROWTH CONTROL AND MALIGNANT TRANSFORMATION
    James Quigley; Fiscal Year: 1992
    ..Using the well-defined primary culture system of normal chick embryo fibroblasts (CEF) transformed by Rous sarcoma virus (RSV), we propose to examine the cellular regulation of PA ..
  60. NEUROPROTECTION AGAINST RETINAL ISCHEMIA
    John Olney; Fiscal Year: 2001
    ..Using an ex vivo chick embryo retina model, the applicants have generated a great deal of information pertaining to underlying mechanisms and ..
  61. EFFECTS OF DIOXIN ON CORONARY ANGIOGENESIS
    Mary Walker; Fiscal Year: 2002
    ..Using the chick embryo as a model, we will test the hypothesis that TCDD inhibits coronary angiogenesis by inhibiting hypoxia-induced ..
  62. Activity and synaptic maturation in the spinal cord
    Peter Wenner; Fiscal Year: 2006
    ..To this end we will block spiking activity in the motor network or in individual spinal neurons in ovo in the chick embryo. By comparing the strength of the synaptic inputs in activity-blocked and control neurons we will test the role ..
  63. SOURCE OF LIMB-FORMING ABILITY IN THE CHICK EMBRYO FLANK
    TRENT STEPHENS; Fiscal Year: 1991
    ..If barriers between the flank and the adjacent limbs inhibit limb formation in the explanted flank, the concept that the flank's limbness qualities originate in the adjacent limbs will be supported...