membrane microdomains

Summary

Summary: Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.

Top Publications

  1. ncbi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
  2. ncbi Revitalizing membrane rafts: new tools and insights
    Kai Simons
    Max Planck Institute of Cell Biology and Genetics, Dresden, Germany
    Nat Rev Mol Cell Biol 11:688-99. 2010
  3. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
  4. ncbi Tetraspanin functions and associated microdomains
    Martin E Hemler
    Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 6:801-11. 2005
  5. ncbi Lipid rafts and signal transduction
    K Simons
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 110, D 01307 Dresden, Germany
    Nat Rev Mol Cell Biol 1:31-9. 2000
  6. ncbi Loss of caveolae, vascular dysfunction, and pulmonary defects in caveolin-1 gene-disrupted mice
    M Drab
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauer Strasse 108, D 01307 Dresden, Germany
    Science 293:2449-52. 2001
  7. ncbi Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells
    Khalid Al-Nedawi
    Montreal Children s Hospital Research Institute, McGill University, 4060 Ste Catherine West, Montreal, Quebec, Canada H3Z 2Z3
    Nat Cell Biol 10:619-24. 2008
  8. pmc PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomains
    Xinxin Gao
    Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 108:14509-14. 2011
  9. pmc Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts
    Robert Ehehalt
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    J Cell Biol 160:113-23. 2003
  10. ncbi Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover
    Gianni M Di Guglielmo
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Cell Biol 5:410-21. 2003

Detail Information

Publications389 found, 100 shown here

  1. ncbi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
    ..Here we review the evidence for how this principle combines the potential for sphingolipid-cholesterol self-assembly with protein specificity to selectively focus membrane bioactivity...
  2. ncbi Revitalizing membrane rafts: new tools and insights
    Kai Simons
    Max Planck Institute of Cell Biology and Genetics, Dresden, Germany
    Nat Rev Mol Cell Biol 11:688-99. 2010
    ....
  3. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  4. ncbi Tetraspanin functions and associated microdomains
    Martin E Hemler
    Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 6:801-11. 2005
    ..These new findings provide a framework for better understanding of these mysterious molecules in the regulation of cellular processes, from signalling to motility...
  5. ncbi Lipid rafts and signal transduction
    K Simons
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 110, D 01307 Dresden, Germany
    Nat Rev Mol Cell Biol 1:31-9. 2000
    ..Lipid rafts containing a given set of proteins can change their size and composition in response to intra- or extracellular stimuli. This favours specific protein-protein interactions, resulting in the activation of signalling cascades...
  6. ncbi Loss of caveolae, vascular dysfunction, and pulmonary defects in caveolin-1 gene-disrupted mice
    M Drab
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauer Strasse 108, D 01307 Dresden, Germany
    Science 293:2449-52. 2001
    ..Thus, caveolin-1 and caveolae play a fundamental role in organizing multiple signaling pathways in the cell...
  7. ncbi Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells
    Khalid Al-Nedawi
    Montreal Children s Hospital Research Institute, McGill University, 4060 Ste Catherine West, Montreal, Quebec, Canada H3Z 2Z3
    Nat Cell Biol 10:619-24. 2008
    ..Thus, membrane microvesicles of cancer cells can contribute to a horizontal propagation of oncogenes and their associated transforming phenotype among subsets of cancer cells...
  8. pmc PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomains
    Xinxin Gao
    Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 108:14509-14. 2011
    ..The phosphatidylinositol 3-kinase (PI3K)/Akt pathway is known to be compartmentalized within plasma membrane microdomains; however, the underlying mechanisms and functional impact of this compartmentalization are not well ..
  9. pmc Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts
    Robert Ehehalt
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    J Cell Biol 160:113-23. 2003
    ..Thus, access of alpha- and beta-secretase to APP, and therefore A beta generation, may be determined by dynamic interactions of APP with lipid rafts...
  10. ncbi Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover
    Gianni M Di Guglielmo
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Cell Biol 5:410-21. 2003
    ..Thus, segregation of TGF-beta receptors into distinct endocytic compartments regulates Smad activation and receptor turnover...
  11. pmc TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation
    Björn F Lillemeier
    Howard Hughes Medical Institute, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA
    Nat Immunol 11:90-6. 2010
    ..This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction...
  12. ncbi Clathrin- and non-clathrin-mediated endocytic regulation of cell signalling
    Christine Le Roy
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Room 1075, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nat Rev Mol Cell Biol 6:112-26. 2005
    ..This functions to control the activity of signalling cascades and the termination of signalling events, and therefore has a key role in defining how a cell responds to its environment...
  13. pmc LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model
    Javier Alegre-Abarrategui
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
    Hum Mol Genet 18:4022-34. 2009
    ..Using a novel human genomic reporter cellular model, we found LRRK2 to locate to membrane microdomains such as the neck of caveolae, microvilli/filopodia and intraluminal vesicles of multivesicular bodies (..
  14. pmc Plasma membrane rafts play a critical role in HIV-1 assembly and release
    A Ono
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 4, Room 307, 9000 Rockville Pike, Bethesda, MD 20892-0460, USA
    Proc Natl Acad Sci U S A 98:13925-30. 2001
    ..These results identify the association of Gag with plasma membrane rafts as an important step in HIV-1 replication. These findings may lead to novel strategies for suppressing HIV-1 replication in vivo...
  15. ncbi Docosahexaenoic acid: membrane properties of a unique fatty acid
    William Stillwell
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Chem Phys Lipids 126:1-27. 2003
    ..It is concluded that DHA's interaction with other membrane lipids, particularly cholesterol, may play a prominent role in modulating the local structure and function of cell membranes...
  16. pmc Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin
    Jeanne Kwik
    Biology Department, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 100:13964-9. 2003
    ..Consistent with this observation, agents that sequester plasma membrane PI(4,5)P2 mimic the effects of cholesterol depletion on actin organization and on lateral mobility...
  17. ncbi Complexes of tetraspanins with integrins: more than meets the eye
    F Berditchevski
    CRC Institute for Cancer Studies, The University of Birmingham, Edgbaston, Birmingham, B15 2TA, UK
    J Cell Sci 114:4143-51. 2001
    ....
  18. pmc Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement
    Sylvain Raffaele
    Laboratoire de Biogenese Membranaire, Unité Mixte de Recherche 5200, Centre National de la Recherche Scientifique University of Bordeaux, Bordeaux 33076, France
    Plant Cell 21:1541-55. 2009
    ..Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane...
  19. ncbi Hierarchical mesoscale domain organization of the plasma membrane
    Akihiro Kusumi
    Institute for Integrated Cell Material Sciences iCeMS, Kyoto University, Kyoto 606 8507, Japan
    Trends Biochem Sci 36:604-15. 2011
    ....
  20. ncbi Lipid rafts and caveolin-1 are required for invadopodia formation and extracellular matrix degradation by human breast cancer cells
    Hideki Yamaguchi
    Laboratory of Genome and Biosignal, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
    Cancer Res 69:8594-602. 2009
    ..In this study, the role of lipid rafts, which are cholesterol-enriched membrane microdomains, in the assembly and function of invadopodia in human breast cancer cells was investigated...
  21. ncbi Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells
    Oleg O Glebov
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Cell Biol 8:46-54. 2006
    ..We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells...
  22. pmc Invasion of Cryptococcus neoformans into human brain microvascular endothelial cells is mediated through the lipid rafts-endocytic pathway via the dual specificity tyrosine phosphorylation-regulated kinase 3 (DYRK3)
    Sheng He Huang
    Department of Pediatrics, Saban Research Institute, Children s Hospital Los Angeles, Los Angeles, California 90027, USA
    J Biol Chem 286:34761-9. 2011
    ..Our data demonstrate that the lipid raft-dependent endocytosis process mediates C. neoformans internalization into HBMEC and that the CD44 protein of the hosts, cytoskeleton, and intracellular kinase-DYRK3 are involved in this process...
  23. ncbi Dissecting lipid raft facilitated cell signaling pathways in cancer
    Samir Kumar Patra
    Cancer Epigenetics Research, Kalyani B 7 183, Nadia, West Bengal, India 741235
    Biochim Biophys Acta 1785:182-206. 2008
    ....
  24. ncbi Scaffolding microdomains and beyond: the function of reggie/flotillin proteins
    M F Langhorst
    Developmental Neurobiology Group, Department of Biology, University of Konstanz, Universitatstrasse 10, 78457, Konstanz, Germany
    Cell Mol Life Sci 62:2228-40. 2005
    ..Since, under certain circumstances, reggie-2/flotillin-1 translocates to the nucleus, reggie/ flotillin microdomains are not only stable scaffolds but also dynamic units with their own regulatory functions...
  25. pmc Elevated levels of cholesterol-rich lipid rafts in cancer cells are correlated with apoptosis sensitivity induced by cholesterol-depleting agents
    Ying Chun Li
    Division of Specific Organs Cancer, Pediatric Oncology Division, National Cancer Center, 809 Madu 1 dong, Ilsan gu, Goyang si, Gyeonggi do 411 769, Korea
    Am J Pathol 168:1107-18; quiz 1404-5. 2006
    ..These results indicate that cancer cells contain increased levels of rafts and suggest a potential use of raft-modulating agents as anti-cancer drugs...
  26. ncbi Lipid rafts, caveolae, and their endocytosis
    Patrick Lajoie
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA
    Int Rev Cell Mol Biol 282:135-63. 2010
    Lipid rafts are plasma membrane microdomains enriched in cholesterol and sphingolipids that are involved in the lateral compartmentalization of molecules at the cell surface...
  27. pmc Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes
    Kulandaivelu S Vetrivel
    Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, Illinois 60637, USA
    J Biol Chem 279:44945-54. 2004
    ..There is growing evidence that cholesterol- and sphingolipid-rich membrane microdomains are involved in regulating trafficking and processing of APP...
  28. ncbi Evaluation of prototype transmembrane 4 superfamily protein complexes and their relation to lipid rafts
    C Claas
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:7974-84. 2001
    ....
  29. ncbi Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation
    Ian Del Conde
    Thrombosis Research Section, Department of Medicine, Baylor College of Medicine, One Baylor Plaza, N 1317, Houston, TX 77030, USA
    Blood 106:1604-11. 2005
    ..enriched in both TF and PSGL-1, and deficient in CD45, suggesting that they arise from distinct membrane microdomains. We investigated the possibility that microvesicles arise from cholesterol-rich lipid rafts and found that ..
  30. pmc Cellular prion protein regulates beta-secretase cleavage of the Alzheimer's amyloid precursor protein
    Edward T Parkin
    Proteolysis Research Group, Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, and Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, Leeds LS2 9JT, United Kingdom
    Proc Natl Acad Sci U S A 104:11062-7. 2007
    ..In conclusion, this is a mechanism by which the cellular production of the neurotoxic Abeta is regulated by PrP(C) and may have implications for both Alzheimer's and prion diseases...
  31. pmc Lattices, rafts, and scaffolds: domain regulation of receptor signaling at the plasma membrane
    Patrick Lajoie
    Deptartment of Cellular and Physiological Sciences, Life Sciences Institute University of British Columbia, Vancouver, British Columbia, Canada
    J Cell Biol 185:381-5. 2009
    ..We review evidence that these heterogeneous microdomains interact to regulate substratum adhesion and cytokine receptor dynamics at the cell surface...
  32. pmc Order of lipid phases in model and plasma membranes
    Hermann Josef Kaiser
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 106:16645-50. 2009
    ..Thus, lipid-mediated coalescence of the GM1 raft domain seems to be distinct from the formation of a Lo phase, suggesting additional interactions between proteins and lipids to be effective...
  33. pmc Cholesterol lipids of Borrelia burgdorferi form lipid rafts and are required for the bactericidal activity of a complement-independent antibody
    Timothy J LaRocca
    Department of Molecular Genetics and Microbiology, and Center for Infectious Diseases, Stony Brook University, Stony Brook, NY 11794, USA
    Cell Host Microbe 8:331-42. 2010
    ..burgdorferi lipid raft organization may have significant implications in the transmission cycle of the spirochetes which are exposed to a range of temperatures...
  34. pmc Research advances on flotillins
    Feng Zhao
    State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046 Gansu, P R China
    Virol J 8:479. 2011
    ....
  35. ncbi High-density lipoprotein reduces the human monocyte inflammatory response
    Andrew J Murphy
    Laboratories of Vascular Pharmacology, Research Institute, Melbourne, Victoria, Australia
    Arterioscler Thromb Vasc Biol 28:2071-7. 2008
    ..Whereas the anti-inflammatory effects of high-density lipoprotein (HDL) on endothelial cells are well described, such effects on monocytes are less studied...
  36. pmc Lipid packing drives the segregation of transmembrane helices into disordered lipid domains in model membranes
    Lars V Schäfer
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
    Proc Natl Acad Sci U S A 108:1343-8. 2011
    ....
  37. pmc Virus entry, assembly, budding, and membrane rafts
    Nathalie Chazal
    Immunologie Virologie, EA 3038, Universite Paul Sabatier, 31062 Toulouse, France
    Microbiol Mol Biol Rev 67:226-37, table of contents. 2003
    ....
  38. ncbi Flotillins and the PHB domain protein family: rafts, worms and anaesthetics
    Isabel C Morrow
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland 4072, Australia
    Traffic 6:725-40. 2005
    ..This review will discuss recent insights into the cell biology of flotillins and the large family of evolutionarily conserved PHB domain proteins...
  39. ncbi The multiple faces of caveolae
    Robert G Parton
    Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland 4072, Australia
    Nat Rev Mol Cell Biol 8:185-94. 2007
    ....
  40. pmc Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol
    Sarah L Veatch
    Departments of Chemistry and Physics, University of Washington, Seattle, Washington 98195 1700, USA
    Biophys J 85:3074-83. 2003
    ..To date, we have not observed macroscopic separation of liquid phases in only binary lipid mixtures...
  41. ncbi Rafts defined: a report on the Keystone Symposium on Lipid Rafts and Cell Function
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:1597-8. 2006
    ..This definition helps to clarify current thinking in a field that has been plagued by the heterogeneous and sometimes ephemeral nature of its subject...
  42. pmc Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains
    Britta Brugger
    Heidelberg University Biochemistry Center BZH, Heidelberg, Germany
    Retrovirology 4:70. 2007
    ..of classical raft lipids and thus identified HIV-1 virions as an example for naturally occurring membrane microdomains. Since Nef modulates the protein composition and function of membrane microdomains we tested here if Nef ..
  43. ncbi The lipid raft-anchored adaptor protein Cbp controls the oncogenic potential of c-Src
    Chitose Oneyama
    Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Cell 30:426-36. 2008
    ..In some human cancer cells and tumors, Cbp is downregulated and the introduction of Cbp significantly suppresses tumorigenesis. These findings indicate a potential role for Cbp as a suppressor of c-Src-mediated tumor progression...
  44. ncbi Lipid raft-associated protein sorting in exosomes
    Aude de Gassart
    UMR 5539, Univ Montpellier II cc107, Montpellier 34095, France
    Blood 102:4336-44. 2003
    ..All together, the data highlight the presence of lipid microdomains in exosomal membranes and suggest their participation in vesicle formation and structure, as well as the direct implication of exosomes in regulatory mechanisms...
  45. pmc Functional role of glycosphingolipids and gangliosides in control of cell adhesion, motility, and growth, through glycosynaptic microdomains
    Adriane Regina Todeschini
    Division of Biomembrane Research, Pacific Northwest Research Institute, University of Washington, Seattle, WA, USA
    Biochim Biophys Acta 1780:421-33. 2008
    ..Data present here suggest that the organizational status of glycosynapse strongly affects cellular phenotype influencing tumor cell malignancy...
  46. pmc The molecular face of lipid rafts in model membranes
    H Jelger Risselada
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Proc Natl Acad Sci U S A 105:17367-72. 2008
    ..At the level of molecular detail, raft-like lipid mixtures show a surprising face with possible implications for many cell membrane processes...
  47. ncbi Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation
    Martha Triantafilou
    University of Portsmouth, School of Biological Sciences, King Henry Building, King Henry I Street, Portsmouth, PO1 2DY, UK
    J Cell Sci 115:2603-11. 2002
    The plasma membrane of cells is composed of lateral heterogeneities, patches and microdomains. These membrane microdomains or lipid rafts are enriched in glycosphingolipids and cholesterol and have been implicated in cellular processes ..
  48. ncbi Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells
    Katerina Malinska
    Universitat Regensburg, Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universitatsstrasse 31, 93040 Regensburg, Germany
    J Cell Sci 117:6031-41. 2004
    ..Finally, two other proteins were localized within the frame of the Can1p/Pma1p plasma-membrane partition. We show that Fur4p (another H+ symporter) and Sur7p (a protein of unknown function) occupy the Can1p subdomain...
  49. pmc Plasma membrane microdomains regulate turnover of transport proteins in yeast
    Guido Grossmann
    Institute of Cell Biology and Plant Physiology, University of Regensburg, 93053 Regensburg, Germany
    J Cell Biol 183:1075-88. 2008
    ..Addition of arginine to wild-type cells leads to a similar redistribution and increased turnover of Can1. Thus, MCC represents a protective area within the plasma membrane to control turnover of transport proteins...
  50. pmc Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae
    Eva Maria Damm
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, CH 8093 Zurich, Switzerland
    J Cell Biol 168:477-88. 2005
    ..Our results revealed the existence of a virus-activated endocytic pathway from the plasma membrane to the ER that involves neither clathrin nor caveolae and that can be activated also in the presence of cav-1...
  51. ncbi Lipid raft microdomains and neurotransmitter signalling
    John A Allen
    Department of Physiology and Biophysics, University of Illinois at Chicago, College of Medicine, Chicago, Illinois, USA
    Nat Rev Neurosci 8:128-40. 2007
    ..The effect of lipid rafts on neurotransmitter signalling has also been implicated in neurological and psychiatric diseases...
  52. pmc Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly
    Jamil S Saad
    Howard Hughes Medical Institute, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA
    Proc Natl Acad Sci U S A 103:11364-9. 2006
    ..Our findings indicate that PI(4,5)P(2) acts as both a trigger of the myristyl switch and a membrane anchor and suggest a potential mechanism for targeting Gag to membrane rafts...
  53. ncbi ATP-binding cassette transporter A1 expression disrupts raft membrane microdomains through its ATPase-related functions
    Yves D Landry
    Ottawa Health Research Institute and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario K1Y 4E9, Canada
    J Biol Chem 281:36091-101. 2006
    ..Such reorganization by ABCA1 effectively expands the non-raft membrane fractions and, consequentially, pre-conditions cells for cholesterol efflux...
  54. ncbi Membrane lipid domains distinct from cholesterol/sphingomyelin-rich rafts are involved in the ABCA1-mediated lipid secretory pathway
    A J Mendez
    University of Miami School of Medicine, Diabetes Research Institute, Miami, Florida 33101, USA
    J Biol Chem 276:3158-66. 2001
    ..We conclude that cholesterol and sphingomyelin-rich membrane rafts do not provide lipid for efflux promoted by apolipoproteins through the ABCA1-mediated lipid secretory pathway and that ABCA1 is not associated with these domains...
  55. ncbi Cholesterol and prostate cancer
    Michael R Freeman
    The Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Cell Biochem 91:54-69. 2004
    ....
  56. pmc Lipid rafts: heterogeneity on the high seas
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid Avenue, Box 8231, St Louis, MO 63110, USA
    Biochem J 378:281-92. 2004
    Lipid rafts are membrane microdomains that are enriched in cholesterol and glycosphingolipids. They have been implicated in processes as diverse as signal transduction, endocytosis and cholesterol trafficking...
  57. pmc Fatty acids modulate Toll-like receptor 4 activation through regulation of receptor dimerization and recruitment into lipid rafts in a reactive oxygen species-dependent manner
    Scott W Wong
    Western Human Nutrition Research Center, the Agricultural Research Service ARS United States Department of Agriculture, and Department of Nutrition, University of California, Davis, California 95616, USA
    J Biol Chem 284:27384-92. 2009
    ....
  58. ncbi Detergent-resistant membranes should not be identified with membrane rafts
    Dov Lichtenberg
    Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Israel
    Trends Biochem Sci 30:430-6. 2005
    ..Because much of what we think we know about lipid rafts is based on their unjustified identification as DRMs, functional domains in biological membranes might differ markedly from the generally accepted picture...
  59. ncbi Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes
    J F Dermine
    , , C.P. 6128, Succ. Centre Ville, , QC, Canada
    J Biol Chem 276:18507-12. 2001
    ..These results indicate that specialized functions required for phagolysosome biogenesis may occur at focal points on the phagosome membrane, and therefore represent a potential target of intracellular pathogens...
  60. ncbi Raft ceramide in molecular medicine
    Erich Gulbins
    Department of Molecular Biology, University of Duisburg Essen, Hufelandstrasse 55, Essen 45122, Germany
    Oncogene 22:7070-7. 2003
    ....
  61. ncbi Detergent resistance as a tool in membrane research
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Nat Protoc 2:2159-65. 2007
    ..These protocols are completed in 1-2 d...
  62. pmc Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta)-dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane
    Fredrick D Oakley
    Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 284:33255-64. 2009
    ..Our results fill in an important mechanistic gap in the understanding of early IL-1R1 and Nox2 signaling events that control NFkappaB activation, a redox-dependent process important in inflammation...
  63. pmc Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression
    Carolin Neumann-Giesen
    Institute of Biochemistry II, Medical School, University of Frankfurt, Theodor Stern Kai 7, D 60590 Frankfurt am Main, Germany
    Biochem J 378:509-18. 2004
    ..Overexpression of reggie-1 resulted in the induction of numerous filopodia-like protrusions in various cell lines, suggesting a role for reggie-1 as a signalling protein in actin-dependent processes...
  64. ncbi Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha-mediated NF-kappaB activation
    Daniel F Legler
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, CH 1066 Epalinges, Switzerland
    Immunity 18:655-64. 2003
    ..Here we show that upon TNFalpha binding, TNFR1 translocates to cholesterol- and sphingolipid-enriched membrane microdomains, termed lipid rafts, where it associates with the Ser/Thr kinase RIP and the adaptor proteins TRADD and ..
  65. ncbi Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2
    Matthias F Langhorst
    Department of Biology, University of Konstanz, Universitatsstrasse 10, D 78457 Konstanz, Germany
    Eur J Cell Biol 87:211-26. 2008
    The reggie/flotillin proteins oligomerize and associate into clusters which form scaffolds for membrane microdomains. Besides their localization at the plasma membrane, the reggies/flotillins reside at various intracellular compartments; ..
  66. ncbi Lipid raft organization and function in brush borders of epithelial cells
    E Michael Danielsen
    Department of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, Denmark
    Mol Membr Biol 23:71-9. 2006
    ..Thus, anti-glycosyl antibodies may serve as guardians of glycolipid-based rafts, protecting them from lumenal pathogens and in this way be part of an ongoing 'cross-talk' between indigenous bacteria and the host...
  67. pmc Direct visualization of Ras proteins in spatially distinct cell surface microdomains
    Ian A Prior
    Department of Pathology and Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4006, Australia
    J Cell Biol 160:165-70. 2003
    ..Differential spatial localization within this framework can likely account for the distinct signal outputs from the highly homologous Ras proteins...
  68. ncbi Functions of lipid raft membrane microdomains at the blood-brain barrier
    Aurore Dodelet-Devillers
    Neuroimmunology Research Laboratory, Center of Excellence in Neuromics, CHUM Notre Dame Hospital, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada
    J Mol Med (Berl) 87:765-74. 2009
    ..In this manuscript, we review the role of membrane microdomains, in particular lipid rafts, in the BBB under physiological conditions and during leukocyte transmigration/..
  69. ncbi Lipid raft clustering and redox signaling platform formation in coronary arterial endothelial cells
    Andrew Y Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, USA
    Hypertension 47:74-80. 2006
    ..These results suggest that LR clustering occurs in coronary endothelial cells. The formation of redox signaling platforms on the cell membrane mediates transmembrane signaling of death receptors, resulting in endothelial dysfunction...
  70. pmc Macrophage ABCA1 reduces MyD88-dependent Toll-like receptor trafficking to lipid rafts by reduction of lipid raft cholesterol
    Xuewei Zhu
    Departments of Pathology Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 51:3196-206. 2010
    ..Collectively, our data suggest that macrophage ABCA1 dampens inflammation by reducing MyD88-dependent TLRs trafficking to lipid rafts by selective reduction of FC content in lipid rafts...
  71. pmc Plasma membrane microdomains regulate TACE-dependent TNFR1 shedding in human endothelial cells
    Alessio D'Alessio
    Istituto Pasteur Fondazione Cenci Bolognetti, Department of Anatomy, Histology, Forensic Medicine and Orthopedics Section of Histology and Medical Embryology, Sapienza University of Rome, Rome, Italy
    J Cell Mol Med 16:627-36. 2012
    ..Surface expressed TNFR1 on EC is largely sequestered into specific plasma membrane microdomains, the lipid rafts/caveolae...
  72. pmc RPTPalpha is essential for NCAM-mediated p59fyn activation and neurite elongation
    Vsevolod Bodrikov
    Zentrum für Molekulare Neurobiologie, Universitat Hamburg, 20246 Hamburg, Germany
    J Cell Biol 168:127-39. 2005
    ....
  73. ncbi Developmental partitioning of myelin basic protein into membrane microdomains
    L S DeBruin
    Department of Molecular and Cellular Biology and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada
    J Neurosci Res 80:211-25. 2005
    Specific membrane microdomains (including lipid rafts) exist in myelin but have not been fully characterized. Myelin basic protein (MBP) maintains the compactness of the myelin sheath and is highly posttranslationally modified...
  74. pmc Relationships between plasma membrane microdomains and HIV-1 assembly
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, USA
    Biol Cell 102:335-50. 2010
    ..Potential roles played by microdomains will be discussed with regard to two post-assembly events, i.e., inhibition of virus release by a raft-associated protein BST-2/tetherin and cell-to-cell HIV-1 transmission at virological synapses...
  75. ncbi The intriguing links between prominin-1 (CD133), cholesterol-based membrane microdomains, remodeling of apical plasma membrane protrusions, extracellular membrane particles, and (neuro)epithelial cell differentiation
    Denis Corbeil
    Tissue Engineering Laboratories, BIOTEC, Technische Universitat Dresden, Dresden, Germany
    FEBS Lett 584:1659-64. 2010
    ..Intriguingly, the release of these particles has been implicated in (neuro)epithelial cell differentiation...
  76. pmc Palmitoylation regulates raft affinity for the majority of integral raft proteins
    Ilya Levental
    Max Planck Institute of Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 107:22050-4. 2010
    ..Because cysteine palmitoylation is the only lipid modification that has been shown to be reversibly regulated, our data suggest a role for palmitoylation as a dynamic raft targeting mechanism for transmembrane proteins...
  77. ncbi HIV entry in macrophages is dependent on intact lipid rafts
    Gemma C Carter
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Virology 386:192-202. 2009
    ..HIV particles were observed co-localising with a marker of lipid rafts (CTB-FITC) early post infection. These data suggest that macrophage membrane cholesterol is essential for HIV entry, and implicate lipid raft involvement...
  78. pmc Low cholesterol triggers membrane microdomain-dependent CD44 shedding and suppresses tumor cell migration
    Toshiyuki Murai
    Department of Immunology and Microbiology, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    J Biol Chem 286:1999-2007. 2011
    ..Our results indicate that membrane lipid organization regulates CD44 shedding and propose a possible molecular mechanism by which cholesterol reduction might be effective for preventing and treating the progression of malignant tumors...
  79. ncbi Effect of the structure of natural sterols and sphingolipids on the formation of ordered sphingolipid/sterol domains (rafts). Comparison of cholesterol to plant, fungal, and disease-associated sterols and comparison of sphingomyelin, cerebrosides, and cer
    X Xu
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794-5215, USA
    J Biol Chem 276:33540-6. 2001
    ..The molecular basis for, and the implications of, the effects of different sterols and sphingolipids (especially ceramide) on the behavior and biological function of rafts are discussed...
  80. ncbi Characterization of the stomatin domain involved in homo-oligomerization and lipid raft association
    Ellen Umlauf
    Max F Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Vienna Biocenter, Vienna A 1030, Austria
    J Biol Chem 281:23349-56. 2006
    ....
  81. pmc Definition of Arabidopsis sterol-rich membrane microdomains by differential treatment with methyl-beta-cyclodextrin and quantitative proteomics
    Sylwia Kierszniowska
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Mol Cell Proteomics 8:612-23. 2009
    ..We were able to distinguish constant and variable components of plant sterol-rich membrane microdomains based on their responsiveness to the drug methyl-beta-cyclodextrin...
  82. ncbi Ceramide-enriched membrane domains
    Claudia R Bollinger
    Department of Molecular Biology, University of Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany
    Biochim Biophys Acta 1746:284-94. 2005
    ....
  83. pmc In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93
    Ilse Delint-Ramirez
    Genes to Cognition Programme, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    J Neurosci 30:8162-70. 2010
    Lipid rafts are dynamic membrane microdomains enriched in cholesterol and sphingolipids involved in the compartmentalization of signaling pathways, trafficking and sorting of proteins...
  84. pmc High resolution mapping of mast cell membranes reveals primary and secondary domains of Fc(epsilon)RI and LAT
    B S Wilson
    Department of Pathology and Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA
    J Cell Biol 154:645-58. 2001
    ..We propose that activated mast cells propagate signals from primary domains organized around Fc(epsilon)RIbeta and from secondary domains, including one organized around LAT...
  85. ncbi Pharmacological inhibition of endocytic pathways: is it specific enough to be useful?
    Andrei I Ivanov
    Department of Medicine, Gastroenterology and Hepatology Division, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
    Methods Mol Biol 440:15-33. 2008
    ..The most and the least-selective inhibitors of each major endocytic pathway are highlighted...
  86. ncbi Lipid rafts as functional heterogeneity in cell membranes
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Biochem Soc Trans 37:955-60. 2009
    ..Evaluation of this property suggests that functional raft heterogeneity arises through principles of lipid-driven phase segregation coupled to additional chemical specificities, probably involving proteins...
  87. ncbi CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation
    Olivier Gaide
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nat Immunol 3:836-43. 2002
    ..Together, our data show that CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation and CD28 costimulation-dependent Jnk activation...
  88. pmc Lateral mobility of nicotinic acetylcholine receptors on neurons is determined by receptor composition, local domain, and cell type
    Catarina C Fernandes
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0357, USA
    J Neurosci 30:8841-51. 2010
    ..The outcome is a system that could tailor nicotinic signaling capabilities to specific needs of individual locations...
  89. pmc Lipid microdomains and the regulation of ion channel function
    Caroline Dart
    Biosciences Building, School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
    J Physiol 588:3169-78. 2010
    ..This brief review highlights some of the existing evidence for raft modulation of channel function...
  90. pmc Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization
    Miguel A Del Pozo
    Centro Nacional de Investigaciones Cardiovasculares CNIC, Madrid 28029, Spain
    Nat Cell Biol 7:901-8. 2005
    ..low-density, cholesterol-rich domains in the plasma membrane named 'lipid rafts' or 'cholesterol-enriched membrane microdomains' (CEMM)...
  91. pmc Cytoskeleton-membrane interactions in membrane raft structure
    Gurunadh R Chichili
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Cell Mol Life Sci 66:2319-28. 2009
    ..We end with a discussion of membrane rafts and the actin cytoskeleton in T cell activation, which function synergistically to initiate the adaptive immune response...
  92. ncbi The fat controller: roles of palmitoylation in intracellular protein trafficking and targeting to membrane microdomains (Review)
    Jennifer Greaves
    The Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, Edinburgh, UK
    Mol Membr Biol 26:67-79. 2009
    ..In this review, we discuss the role of palmitoylation as a versatile protein sorting signal, regulating protein trafficking between distinct intracellular compartments and the micro-localization of proteins within membranes...
  93. pmc Annexin 2 promotes the formation of lipid microdomains required for calcium-regulated exocytosis of dense-core vesicles
    Sylvette Chasserot-Golaz
    Centre National de la Recherche Scientifique, Unité Propre de Recherche 2356, IFR 37 des Neurosciences, 67084 Strasbourg Cedex, France
    Mol Biol Cell 16:1108-19. 2005
    ..We propose that annexin 2 acts as a calcium-dependent promoter of lipid microdomains required for structural and spatial organization of the exocytotic machinery...
  94. pmc Cholesterol and ion channels
    Irena Levitan
    Department of Medicine, University of Illinois at Chicago, Chicago, IL, USA
    Subcell Biochem 51:509-49. 2010
    ..The goal of this review is to describe systematically the role of cholesterol in regulation of the major types of ion channels and to discuss these effects in the context of the models proposed...
  95. ncbi Greasing their way: lipid modifications determine protein association with membrane rafts
    Ilya Levental
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
    Biochemistry 49:6305-16. 2010
    ....
  96. pmc Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains
    Gonzalo P Solis
    Department of Biology, University of Konstanz, 78467 Konstanz, Germany
    Biochem J 403:313-22. 2007
    Reggie-1 and -2 proteins (flotillin-2 and -1 respectively) form their own type of non-caveolar membrane microdomains, which are involved in important cellular processes such as T-cell activation, phagocytosis and signalling mediated by ..
  97. ncbi ABCB19/PGP19 stabilises PIN1 in membrane microdomains in Arabidopsis
    Boosaree Titapiwatanakun
    Department of Horticulture, Purdue University, West Lafayette, IN 47907 2010, USA
    Plant J 57:27-44. 2009
    ..These data suggest that ABCB19 stabilises PIN1 localisation at the plasma membrane in discrete cellular subdomains where PIN1 and ABCB19 expression overlaps...
  98. pmc Lipids and membrane microdomains in HIV-1 replication
    Abdul A Waheed
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA
    Virus Res 143:162-76. 2009
    ..of data indicates that these early and late steps in HIV-1 replication take place in specialized plasma membrane microdomains, and that many of the viral and cellular components required for entry, assembly, and budding are ..
  99. pmc Initial steps of Shigella infection depend on the cholesterol/sphingolipid raft-mediated CD44-IpaB interaction
    Frank Lafont
    Department of Genetics and Microbiology, Centre Medical Universitaire, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    EMBO J 21:4449-57. 2002
    ..hyaluronan receptor, and Shigella, the invasin IpaB, which partitions during infection within specialized membrane microdomains enriched in cholesterol and sphingolipids, called rafts...
  100. pmc Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells
    Waldemar Popik
    Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    J Virol 76:4709-22. 2002
    ..Colocalization of CCR5 with GM1 and gp120 upon engagement of CD4 and CXCR4 by HIV-1 further supports our observation that HIV-1 receptors localize to the same lipid rafts in PM1 T cells...
  101. pmc The challenge of lipid rafts
    Linda J Pike
    Washington University School of Medicine Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
    J Lipid Res 50:S323-8. 2009
    ..It also outlines the contribution of single-molecule methods in defining the forces that drive the formation and dynamics of these membrane domains...

Research Grants66

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..molecular events that initiate allergic inflammation and more generally into the nature and assembly of membrane microdomains controlling signal transduction and membrane trafficking in immune cells...
  2. TARGETING THE MARCKS-FAMILY PROTEINS TO INHIBIT NEUTROPHIL MIGRATION
    Mary K Sheats; Fiscal Year: 2013
    ..7 cells, maintenance of PIP2 cell membrane microdomains and dynamic actin structure formation in the neuron-like cell line PC12 and membrane ruffling and lamellae ..
  3. Modulation of host immune defense by Pneumocystis beta-glucans
    EVA MARIA CARMONA PORQUERA; Fiscal Year: 2013
    ..The future experiments outlined here will also enable me to move into the field of plasma membrane microdomains and initiate an investigation into individualized responses to vaccination through the latest high ..
  4. Amoeboid Membrane Dynamics in Prostate Cancer
    Michael R Freeman; Fiscal Year: 2013
    ..capable of altering the tumor microenvironment and associates with cholesterol-rich lipid raft membrane microdomains. These data suggest that the DRF3 network may be sensitive to cholesterol- or fatty acid-targeting ..
  5. Roles of Phospholipase D in AT1 Receptor Endocytosis
    Guangwei Du; Fiscal Year: 2010
    ..We will also examine whether membrane microdomains regulated by PLD2 signaling are critical for CCP formation...
  6. Spatiotemporal Regulation of Membrane Raft Trafficking in Virus Activated Cells
    Tione Buranda; Fiscal Year: 2012
    ..the cellular machinery of endocytosis to enter and infect cells through the mediation of specialized membrane microdomains called lipid rafts...
  7. EICOSANOIDS AND LUNG MACROPHAGE ANTIMICROBIAL MECHANISMS
    MARC L PETERS-GOLDEN; Fiscal Year: 2012
    ..cysLT1 and EP2 vs. EP4 reflect the differential localization of these receptors to lipid raft membrane microdomains and their differential coupling to two distinct cyclic AMP effectors - protein kinase A (PKA) and exchange ..
  8. Allosteric Regulation of SNAREs and the Rab GEF Mon1 by Phosphoinositides
    Rutilio A Fratti; Fiscal Year: 2013
    ..Fusion occurs at membrane microdomains where regulatory lipids interdependently assemble with the proteins that catalyze fusion including SNAREs, ..
  9. Estrogen regulation of inflammatory mediator signaling
    William P Clarke; Fiscal Year: 2011
    ..changes in signal transduction efficiency (receptor-G protein coupling efficiency or trafficking to/from membrane microdomains (lipid rafts)...
  10. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2010
    ..Caveolae are specialized membrane microdomains that serve as platforms for integrating specific cellular signal transduction processes...
  11. ASSEMBLY OF ENVELOPED VIRUSES
    Douglas S Lyles; Fiscal Year: 2013
    ..movement of viral nucleocapsids to membrane-proximal regions of the cytoplasm, where they associate with membrane microdomains that are enriched not in M protein, but rather in G protein...
  12. Membrane Remodeling and Protein Trafficking in Bacteria
    David Z Rudner; Fiscal Year: 2013
    ..4) Investigate how membrane protein localization and dynamics are influenced by membrane composition, membrane microdomains, and the MreB cytoskeleton...
  13. Lung epithelium collaborates with alveolar macrophages in host defense
    Min Wu; Fiscal Year: 2012
    ..study the underlying mechanism for MCP-1 secretion and hopefully identify the involvement of ceramide-rich membrane microdomains. Acid shingomyelinase will be blocked by siRNA and chemical inhibitors for analyzing sphingolipid ..
  14. Direct effect of cholesterol on glucose-stimulated insulin secretion
    Mingming Hao; Fiscal Year: 2010
    ..in GSIS, neuronal nitric oxide synthase (nNOS) and glucokinase (GK), depends on cholesterol-enriched membrane microdomains on the insulin granules...
  15. Functional Role of the Tetraspanin CD82/Kai1 in Innate and Adaptive Immunity
    Jatin M Vyas; Fiscal Year: 2013
    ..times and are thought to play a structural role, interacting laterally with other proteins and forming membrane microdomains. Characterization of precise function of tetraspanins has been notoriously difficult as a result of ..
  16. Contribution of caveolin to diabetic vascular dysfunction
    Kathryn G Lamping; Fiscal Year: 2013
    ..serotonergic receptors and rhoA have been found associated with caveolin-1 in specialized plasma membrane microdomains of VSM...
  17. Computational modeling of viral assembly: encapsulation of nucleic acids and env
    Michael F Hagan; Fiscal Year: 2013
    ..as protein interactions and membrane rigidity, and why many viruses preferentially bud from particular membrane microdomains. Predictions will be compared to experiments on alphavirus budding...
  18. Ordered Membrane Domain Formation and Function in Pathogenic Bacteria
    Erwin London; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The formation, properties and biological functions of membrane microdomains rich in cholesterol is one of the most exciting and controversial topics in membrane structure and function...
  19. Regulation of Metabotropic glutamate Receptor Signaling by Caveolar Rafts
    Anna Francesconi; Fiscal Year: 2013
    ..Lipid rafts and caveolae are specialized membrane microdomains that serve as platforms to compartmentalize signaling activities at the cell surface...
  20. Fatty Acid Synthase, Caveolin-1 and Membrane Microdomains in Prostate Cancer
    Dolores Di Vizio; Fiscal Year: 2012
    ..abstract_text> ..
  21. Discovery of novel cholera toxin receptors
    JENNIFER KOHLER; Fiscal Year: 2012
    ..knowledge of CTxB binding partners will be vital to defining the composition and characteristics of membrane microdomains. PUBLIC HEALTH RELEVANCE: The bacteria Vibrio cholerae produce a toxin that binds to and invades human ..
  22. Renal Dopamine Receptor and Regulation
    Pedro A Jose; Fiscal Year: 2013
    ..We hypothesize that the assembly of DIR with the signaling complex in membrane microdomains is govemed by SNXS...
  23. TRP and AQP Channels: Modulation of Function, Raft Location by Membrane Lipids
    THOMAS J MC INTOSH; Fiscal Year: 2012
    ..Moreover, some channels are sequestered into cholesterol-rich plasma membrane microdomains called rafts...
  24. Spatial Regulation of RGS and G Protein Signaling
    Marilyn Gist Farquhar; Fiscal Year: 2013
    ..signaling;and 6) Discovery of a new paradigm for spatial regulation of G1i signaling in clathrin-coated membrane microdomains. The overall goal of the work proposed is to further define two of these novel pathways we have discovered-..
  25. UTERINE RELAXING FACTORS: MOLECULAR ASPECTS OF ACTION
    BARBARA SANBORN; Fiscal Year: 2009
    ..how the constituents of OTR and cAMP regulatory pathways and K(Ca) channels are distributed between plasma membrane microdomains and assess whether distribution is affected by contractant or relaxant signals to influence signaling ..
  26. OFD1 as constituent of a multimeric protein complex in odontoblast primary cilia
    STEPHANIE JUSTINE JERMAN; Fiscal Year: 2013
    ..odontoblast and renal epithelial cells using a conserved trafficking mechanism where it is organized into membrane microdomains with specialized signaling functions. Two specific aims are proposed...
  27. REGULATION OF UTERINE SMOOTH MUSCLE EXCITABILITY
    Sarah K England; Fiscal Year: 2010
    ..sensitivity to intracellular regulators and potentially also in their localization to distinct plasma membrane microdomains (including lipid rafts and caveolae) in which signaling molecules and their effector proteins are co-..
  28. Dopamine Transporter Interactions: Understanding Transporter Function
    PETER JAMES HAMILTON; Fiscal Year: 2013
    ..the membrane raft-associated protein Flotillin-1 (Flot1) is necessary for the localization of DAT to these membrane microdomains. Flot1 knockdown, which displaces DAT from membrane rafts, blunts AMPH-induced DA efflux...
  29. Amyloidogenic Processing of APP
    Gopal Thinakaran; Fiscal Year: 2013
    ..Evidence from a variety of in vitro and in vivo studies indicates that cholesterol- and sphingolipid-rich membrane microdomains, termed lipid rafts, might be a critical link between cellular cholesterol levels and amyloidogenic ..
  30. ApicalMembrane Modeling in Tubulogenesis
    VERENA GOBEL; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Apical membrane modeling shapes organ lumina, defines membrane microdomains and leads cell movement during epithelial morphogenesis...
  31. Caveolae and Cardiac Repolarization
    TIMOTHY KAMP; Fiscal Year: 2013
    ..Cav-3 is an essential scaffolding protein required for the formation of specialized membrane microdomains in cells referred to as caveolae which are home to multiple signaling molecules and ion channel proteins...
  32. FASEB SRC on Protein Lipidation, Signaling and Membrane Domains
    Christine A Hrycyna; Fiscal Year: 2011
    ..with effector molecules and downstream signaling partners can be affected by shuttling in and out of membrane microdomains. Thus, investigators in these research areas benefit from and find synergy in discussions and ..
  33. Novel ERG regulation of CXCR4 in prostate cancer progression
    Sreenivasa R Chinni; Fiscal Year: 2013
    ..the mechanism of CXCR4 transactivation of epidermal growth factor family members in lipid raft membrane microdomains. This approach is innovative, because the proposed project is designed to determine whether CXCR4 ..
  34. MOLECULAR BASIS OF GLYCOSPHINGOLIPID BINDING SPECIFICITY
    Dinshaw J Patel; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Glycosphingolipids (GSL)-enriched 'rafts'and caveolae are membrane microdomains that putatively function as lateral organizing sites for signaling proteins involved in oncogenesis...
  35. Ceramide membrane microdomains regulate cytokine secretion
    Brij B Singh; Fiscal Year: 2013
    ..On the other hand, the importance of lipid rafts (membrane microdomains) for innate immunity has been indicated by the fact that CF patients, who are particularly at risk for PA ..
  36. Mechanisms of apoptotic calcium signaling
    DARREN F BOEHNING; Fiscal Year: 2013
    ..Our preliminary data suggests that Fas and TCR components are recruited into detergent-resistant membrane microdomains enriched in cholesterol and glycosphingolipids (lipid rafts) following Fas stimulation...
  37. Mechanisms that determine subcellular sites of HIV-1 assembly
    Akira Ono; Fiscal Year: 2013
    ..Once at the PM, Gag further associates specifically with membrane microdomains known as lipid rafts as well as larger PM domains...
  38. Regulation of Renal Phosphate Transport by Lipids
    Moshe Levi; Fiscal Year: 2013
    ..proposal will test the HYPOTHESES that NaPi-2a, NaPi-2c and PiT-2 are localized in distinct brush border membrane microdomains or nanodomains, resulting in: 1) differential regulation of lateral diffusion and clustering of the NaPi ..
  39. Relationships between HIV-1 assembly and the plasma membrane organization
    Akira Ono; Fiscal Year: 2010
    ..The goals of the proposed research are to determine the effects of HIV-1 particle assembly on the plasma membrane microdomains and to elucidate the consequence of such effects in virus spread...
  40. Glycolipid Trafficking of Cholera Toxin by Lipid Rafts
    David E Saslowsky; Fiscal Year: 2010
    ..Our hypothesis is that such sorting depends on the structure/function of membrane microdomains termed lipid rafts...
  41. How KAI1/CD82 Suppresses Cancer Invasion and Metastasis
    Xin A Zhang; Fiscal Year: 2013
    ..Recent studies indicate that KAI1/CD82 induces the reorganization of membrane microdomains such as lipid rafts and tetraspanin-enriched microdomain (TEM), attenuates growth factor and integrin ..
  42. Membrane Rafts and Sertoli-Germ Cell Interactions
    SALLY NYQUIST; Fiscal Year: 2002
    ..These fractions are enriched in membrane microdomains (rafts), located at the apical surface of the Sertoli cell where interactions with the meiotic and post-..
  43. Mucosal T Cells: Is Tolerance Floating on Lipid Rafts
    Alan Levine; Fiscal Year: 2007
    ..We have also described that cholesterol-rich membrane microdomains, called lipid rafts, are not only structtLral components of the plasma membrane, but also localize ..
  44. LH Receptor C-terminus in Receptor Desensitization
    DEBORAH ROESS; Fiscal Year: 2003
    ..critical for desensitization of the receptor and for targeting of LH receptors to specific plasma membrane microdomains. To evaluate the role of the LH receptor C- terminus, the applicant will use chimeric receptors derived ..
  45. Caveolins, Caveolae, and Lipid Rafts
    DOUGLAS LUBLIN; Fiscal Year: 2004
    ....
  46. Molecular Mechanisms Mediating Resistance to Leptin Signaling During Fat Gain
    Virend Somers; Fiscal Year: 2009
    ..hypothesis that resistance to leptin signaling is a result of molecular and structural changes in caveolar membrane microdomains. To test our hypothesis we propose a series of studies directed at investigating the molecular mechanisms ..
  47. MEMBRANE EVENTS FOLLOWING HORMONE BINDING TO LH RECEPTOR
    DEBORAH ROESS; Fiscal Year: 2005
    ..LH receptors on viable cells will be used to determine whether LH receptors become localized in small membrane microdomains following binding of hormone...
  48. Transport and Lipid Interactions of a Novel Enterotoxin
    Judith Ball; Fiscal Year: 2005
    ..This investigation will contribute new insights into our understanding of the newly discovered plasma membrane microdomains (such as caveolae); broaden our knowledge of intracellular protein-membrane/lipid interactions; contribute ..
  49. The Role of Actin Remodeling in Membrane Fusion Microdomains
    William Wickner; Fiscal Year: 2007
    ..organelle movement, actin remodeling is essential for the assembly and function of docking and fusion membrane microdomains, as shown with the yeast vacuole...
  50. Structure and Function of Membrane Rafts
    Deborah Brown; Fiscal Year: 2009
    Lipid rafts are membrane microdomains present in an ordered phase. These are rich in sphingolipids and cholesterol in mammalian cells...
  51. PHOSPHORYLATION AND SPATIAL LOCALIZATION OF TAU PROTEIN
    Gloria Lee; Fiscal Year: 2004
    ..data suggesting that tau can increase the tyrosine kinase activity of fyn and that tau associates with membrane microdomains, also known as membrane rafts...
  52. NUCLEAR PL SCRAMBLASE IN DIFFERENTIATION & APOPTOSIS
    Peter Sims; Fiscal Year: 2009
    ..By contrast, palmitoylated PLSCR1 is a component of plasma membrane lipid "rafts", membrane microdomains believed to be involved in both assembly of receptor signaling platforms and endocytic trafficking of ..
  53. ANGIOTENSIN II RECEPTOR SIGNALING DOMAINS
    Robert Wayne Alexander; Fiscal Year: 2010
    ..Caveolin-enriched lipid rafts (CE/LRM) are specialized membrane microdomains where signaling molecules such as EGFR are compartmentalized via interacting with caveolin-1 (Cav1)...
  54. The Role of Cholesterol in Regulating NGF Activity
    JOSEPH BARTOE; Fiscal Year: 2004
    ..Using immunohistochemistry and density fractionation of cellular lysates to isolate distinct membrane microdomains, differences in the subcellular localization of receptors in cholesterol-depleted and control cells will ..
  55. Molecular Basis for Overcoming Tolerance to Sm
    BARBARA VILEN; Fiscal Year: 2007
    ..Mechanistically, renewed signal transduction may occur when desensitized receptors repartition to membrane microdomains called lipid rafts...
  56. Fatty Acid Synthase, Caveolin-1 and Membrane Microdomains in Prostate Cancer
    Dolores Di Vizio; Fiscal Year: 2009
    ....
  57. DYNAMIC CAVEOLIN-CAVEOLAE-STEROID SIGNALING COMPLEXES
    SUSAN SADLER; Fiscal Year: 2000
    ..organize and regulate signal transduction in hormone-responsive cells are apparently localized in plasma membrane microdomains that are enriched in cholesterol and other complex lipids...
  58. Role of PLSCR in Cell Response to Growth Factors
    Therese Wiedmer; Fiscal Year: 2007
    ..palmitoylated PLSCR1 is a component of plasma membrane lipid "rafts", cholesterol- and sphingomyelin-rich membrane microdomains that are believed to be involved in both assembly of receptor signaling platforms and the endocytic ..
  59. HIV as Viral Exosome: Role of Rab GTPases and NPC1
    James Hildreth; Fiscal Year: 2005
    ..These membrane microdomains, highly enriched in cholesterol and sphingolipids, serve as platforms for lattices for signaling proteins...
  60. Cytoskeletal Linking Proteins in Liver Function
    R Brian Doctor; Fiscal Year: 2006
    ..proteins to the cytoskeleton and moderate their distribution, retention, clustering and activity within membrane microdomains. In epithelial cells, Ezrin-Radixin-Moesin binding phosphoprotein 50 (EBP50) has been implicated in ..
  61. Lipid Rafts Differentially Regulate T Cell Subsets
    BRENDA RIVERA REYES; Fiscal Year: 2005
    ..cells and our submitted manuscript studying PBT have described the function of cholesterol-rich membrane microdomains, called lipid rafts, as not only structural components of the plasma membrane, but also localized sites ..
  62. Functional Interactions of Mu and Delta Opioid Receptors
    ERICA LEVITT; Fiscal Year: 2009
    ..could be explained by heterodimerization of [unreadable] and d receptors, or by compartmentalization into membrane microdomains, such as lipid rafts, to form functional signaling complexes...
  63. Membrane Organisation by Tetraspanins and Small Multi-transmembrane
    Tung Tien Sun; Fiscal Year: 2006
    ..One exciting new understanding is that[unreadable] tetraspanins form unique membrane microdomains analogous to the glycosphingolipid[unreadable] enriched "rafts" which serve to partition membrane ..
  64. The Role of Ceramide in Diabetic Retinopathy
    Mark Kester; Fiscal Year: 2006
    ..proposal is that altered ceramide metabolism in diabetes leads to insulin receptor dysfunction within membrane microdomains (rafts)...
  65. FASEB Conference-Protein Lipidation and Membrane Domains
    Anne Kenworthy; Fiscal Year: 2009
    ..progress, and provide a needed forum for critical discussions, concerning lipid-modified proteins and membrane microdomains and their roles in processes including signal transduction, trafficking of membrane molecules and disease ..
  66. 2004 Biophysical Discussion: Membrane Microdomains
    Kenneth Jacobson; Fiscal Year: 2004
    The 2004 Biophysical Discussions will be on the topic, "Probing membrane microdomains." The meeting will be held in Asilomar, Monterey, CA from Oct. 28-31, 2004...