3t3 l1 cells

Summary

Summary: A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.

Top Publications

  1. pmc Interleukin-1beta-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression
    Jennifer Jager
    Institut National de la Santé et de la Recherche Médicale INSERM U568, Faculty of Medicine, Avenue de Valombrose, 06107 Nice Cedex 2, France
    Endocrinology 148:241-51. 2007
  2. ncbi Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking
    Mark Larance
    Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, Australia
    J Biol Chem 280:37803-13. 2005
  3. pmc Increased oxidative stress in obesity and its impact on metabolic syndrome
    Shigetada Furukawa
    Department of Medicine and Pathophysiology, Graduate School of Medicine, Department of Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Clin Invest 114:1752-61. 2004
  4. pmc Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone
    Leanne Wilson-Fritch
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01601, USA
    J Clin Invest 114:1281-9. 2004
  5. ncbi Full intracellular retention of GLUT4 requires AS160 Rab GTPase activating protein
    Lorena Eguez
    Department of Biochemistry, Weill Cornell Medical School, New York, New York 10021, USA
    Cell Metab 2:263-72. 2005
  6. ncbi Activation of transient receptor potential vanilloid type-1 channel prevents adipogenesis and obesity
    Li Li Zhang
    Center for Hypertension and Metabolic Diseases, Department of Hypertension and Endocrinology, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China
    Circ Res 100:1063-70. 2007
  7. ncbi Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway
    Cheng Huang
    Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 294 Tai Yuan Road, Shanghai 200031, China
    Biochem Biophys Res Commun 348:571-8. 2006
  8. ncbi RNA interference of PPARgamma using fiber-modified adenovirus vector efficiently suppresses preadipocyte-to-adipocyte differentiation in 3T3-L1 cells
    Tetsuji Hosono
    Division of Cellular and Gene Therapy Products, National Institute of Health Sciences, Tokyo 158 8501, Japan
    Gene 348:157-65. 2005
  9. ncbi Regulation of insulin action by ceramide: dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B
    Suzanne Stratford
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    J Biol Chem 279:36608-15. 2004
  10. pmc Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis
    Qi Qun Tang
    Departments of Pediatrics Division of Endocrinology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:9766-71. 2005

Detail Information

Publications331 found, 100 shown here

  1. pmc Interleukin-1beta-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression
    Jennifer Jager
    Institut National de la Santé et de la Recherche Médicale INSERM U568, Faculty of Medicine, Avenue de Valombrose, 06107 Nice Cedex 2, France
    Endocrinology 148:241-51. 2007
    ..By targeting IRS-1, IL-1beta is capable of impairing insulin signaling and action, and could thus participate in concert with other cytokines, in the development of insulin resistance in adipocytes...
  2. ncbi Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking
    Mark Larance
    Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, Australia
    J Biol Chem 280:37803-13. 2005
    ..These findings support an important role for AS160 in the insulin regulated trafficking of GLUT4...
  3. pmc Increased oxidative stress in obesity and its impact on metabolic syndrome
    Shigetada Furukawa
    Department of Medicine and Pathophysiology, Graduate School of Medicine, Department of Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Clin Invest 114:1752-61. 2004
    ....
  4. pmc Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone
    Leanne Wilson-Fritch
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01601, USA
    J Clin Invest 114:1281-9. 2004
    ....
  5. ncbi Full intracellular retention of GLUT4 requires AS160 Rab GTPase activating protein
    Lorena Eguez
    Department of Biochemistry, Weill Cornell Medical School, New York, New York 10021, USA
    Cell Metab 2:263-72. 2005
    ..Our findings that AS160 knockdown only partially releases basal GLUT4 retention provides evidence that insulin signals to GLUT4 exocytosis by both AS160-dependent and -independent mechanisms...
  6. ncbi Activation of transient receptor potential vanilloid type-1 channel prevents adipogenesis and obesity
    Li Li Zhang
    Center for Hypertension and Metabolic Diseases, Department of Hypertension and Endocrinology, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China
    Circ Res 100:1063-70. 2007
    ..We conclude that the activation of TRPV1 channels by capsaicin prevented adipogenesis and obesity...
  7. ncbi Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway
    Cheng Huang
    Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of CAS, Chinese Academy of Sciences, 294 Tai Yuan Road, Shanghai 200031, China
    Biochem Biophys Res Commun 348:571-8. 2006
    ..These studies suggest that BBR works on multiple molecular targets as an inhibitor of PPARgamma and alpha, and is a potential weight reducing, hypolipidemic, and hypoglycemic drug...
  8. ncbi RNA interference of PPARgamma using fiber-modified adenovirus vector efficiently suppresses preadipocyte-to-adipocyte differentiation in 3T3-L1 cells
    Tetsuji Hosono
    Division of Cellular and Gene Therapy Products, National Institute of Health Sciences, Tokyo 158 8501, Japan
    Gene 348:157-65. 2005
    ....
  9. ncbi Regulation of insulin action by ceramide: dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B
    Suzanne Stratford
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    J Biol Chem 279:36608-15. 2004
    ..Moreover the results obtained provide a unifying theory to account for the numerous dissenting reports investigating the actions of ceramide toward Akt/PKB...
  10. pmc Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis
    Qi Qun Tang
    Departments of Pediatrics Division of Endocrinology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:9766-71. 2005
    ..The delayed transactivation of the C/EBPalpha and PPARgamma genes by C/EBPbeta appears necessary to allow mitotic clonal expansion, which would otherwise be prevented, because C/EBPalpha and PPARgamma are antimitotic...
  11. ncbi CGI-58 facilitates lipolysis on lipid droplets but is not involved in the vesiculation of lipid droplets caused by hormonal stimulation
    Tomohiro Yamaguchi
    Graduate School of Life Science, University of Hyogo, 3 2 1, Koto, Kamigori, Hyogo 678 1297, Japan
    J Lipid Res 48:1078-89. 2007
    ..CGI-58 is not required for this process. Thus, CGI-58 facilitates lipolysis in cooperation with perilipin and other factors, including lipases...
  12. ncbi Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase
    Robert Zimmermann
    Institute of Molecular Biosciences, University of Graz, Graz, Austria
    Science 306:1383-6. 2004
    ..Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals...
  13. pmc PPARgamma and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale
    Martina I Lefterova
    Institute for Diabetes, Obesity, and Metabolism, and Division of Endocrinology, Diabetes, and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 22:2941-52. 2008
    ..Thus, PPARgamma and C/EBP factors cooperatively orchestrate adipocyte biology by adjacent binding on an unanticipated scale...
  14. ncbi Mature-onset obesity and insulin resistance in mice deficient in the signaling adapter p62
    Angelina Rodriguez
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Cell Metab 3:211-22. 2006
    ..We propose that p62 normally antagonizes basal ERK activity and adipocyte differentiation and that its loss leads to the hyperactivation of ERK that favors adipogenesis and obesity...
  15. ncbi Flux profile and modularity analysis of time-dependent metabolic changes of de novo adipocyte formation
    Yaguang Si
    Department of Biology, Tufts University, Medford, MA 02155, USA
    Am J Physiol Endocrinol Metab 292:E1637-46. 2007
    ..Flux and modularity analysis results together point to the flux distribution around pyruvate as a key indicator of adipocyte lipid accumulation...
  16. ncbi The regulation of adipogenesis through GPR120
    Chizu Gotoh
    Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano ken 399 4598, Japan
    Biochem Biophys Res Commun 354:591-7. 2007
    ..Our results suggest that GPR120 regulates adipogenic processes such as adipocyte development and differentiation...
  17. ncbi The adipose tissue triglyceride lipase ATGL/PNPLA2 is downregulated by insulin and TNF-alpha in 3T3-L1 adipocytes and is a target for transactivation by PPARgamma
    Ji Young Kim
    Dept of Biochemistry and Cancer Biology, Medical University of Ohio, Toledo, OH 43614, USA
    Am J Physiol Endocrinol Metab 291:E115-27. 2006
    ..Our findings identify the novel lipase ATGL to be a target gene for TNF-alpha and insulin action in adipocytes and reveal that it is subject to transcriptional control by PPARgamma-mediated signals...
  18. ncbi Real-time quantification of fatty acid uptake using a novel fluorescence assay
    Jinfang Liao
    Molecular Devices Corporation, Sunnyvale, CA 94089, USA
    J Lipid Res 46:597-602. 2005
    ..Applications of this novel assay should facilitate new insights into the biology of fatty acid uptake and provide new means for obesity-related drug discovery...
  19. ncbi The Wnt antagonist Dickkopf-1 and its receptors are coordinately regulated during early human adipogenesis
    Constantinos Christodoulides
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QR, UK
    J Cell Sci 119:2613-20. 2006
    ..Given the public health importance of disorders of adipose mass, further knowledge of the pathways involved specifically in human adipocyte differentiation might ultimately be of clinical relevance...
  20. ncbi Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43
    Yeon Hee Hong
    Department of Food Production Science, Shinshu University, Nagano ken, Japan
    Endocrinology 146:5092-9. 2005
    ..We conclude that acetate and propionate short chain fatty acids may have important physiological roles in adipogenesis through GPCR43, but not through GPCR41...
  21. ncbi Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta during adipogenesis requires a peroxisome proliferator-activated receptor-gamma-associated repression of HDAC1 at the C/ebp alpha gene promoter
    Ying Zuo
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 281:7960-7. 2006
    ....
  22. ncbi Protein kinase B/AKT 1 plays a pivotal role in insulin-like growth factor-1 receptor signaling induced 3T3-L1 adipocyte differentiation
    Jinfei Xu
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    J Biol Chem 279:35914-22. 2004
    ..Thus, protein kinase B/Akt 1 is an important signal mediator in IGF-1 receptor signal cascade for inducing adipocyte differentiation...
  23. ncbi ASP enhances in situ lipoprotein lipase activity by increasing fatty acid trapping in adipocytes
    May Faraj
    Mike Rosenbloom Laboratory for Cardiovascular Research, McGill University Health Center, Montreal, Canada
    J Lipid Res 45:657-66. 2004
    ....
  24. pmc MicroRNA and 3T3-L1 pre-adipocyte differentiation
    Kazuaki Kajimoto
    Department of Epidemiology, Research Institute, National Cardiovascular Center, Suita, Osaka, Japan
    RNA 12:1626-32. 2006
    ..Although these miRNAs may be involved in modulating adipocyte function, mild down-modulations of the up-regulated miRNAs do not appear to affect 3T3-L1 pre-adipocyte differentiation...
  25. ncbi Krüppel-like transcription factor KLF5 is a key regulator of adipocyte differentiation
    Yumiko Oishi
    Department of Cardiovascular Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo, Tokyo 113 8655, Japan
    Cell Metab 1:27-39. 2005
    ..KLF5, in turn, acts in concert with C/EBPbeta/delta to activate the PPARgamma2 promoter. This study establishes KLF5 as a key component of the transcription factor network controlling adipocyte differentiation...
  26. ncbi Effects of oxidative stress on adiponectin secretion and lactate production in 3T3-L1 adipocytes
    A F Soares
    UMR 585 INSERM INSA de Lyon, Pathophysiology of Lipids and Membranes, IMBL, 69621 Villeurbanne Cedex, France
    Free Radic Biol Med 38:882-9. 2005
    ..These results demonstrate that adipose cells are highly sensitive to oxidative stress, with subsequent decreased adiponectin secretion and increased lactate production, two events involved in the development of insulin resistance...
  27. ncbi Standard isolation of primary adipose cells from mouse epididymal fat pads induces inflammatory mediators and down-regulates adipocyte genes
    Hong Ruan
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    J Biol Chem 278:47585-93. 2003
    ....
  28. pmc Cyclic AMP (cAMP)-mediated stimulation of adipocyte differentiation requires the synergistic action of Epac- and cAMP-dependent protein kinase-dependent processes
    Rasmus Koefoed Petersen
    BioLigands, International Science Park Odense, DK 5230 Odense M, Denmark
    Mol Cell Biol 28:3804-16. 2008
    ....
  29. ncbi The peroxisome proliferator-activated receptor gamma regulates expression of the perilipin gene in adipocytes
    Naoto Arimura
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    J Biol Chem 279:10070-6. 2004
    ....
  30. pmc Phosphorylation of C/EBPalpha inhibits granulopoiesis
    Sarah E Ross
    Department of Molecular and Integrative Physiology, University of Michigan Medical School, 1301 E Catherine Street, Ann Arbor, MI 48109 0622, USA
    Mol Cell Biol 24:675-86. 2004
    ..Thus, myeloid development is partly regulated by an ERK1/2-mediated change in the conformation of C/EBPalpha that favors monocyte differentiation by blocking granulopoiesis...
  31. ncbi Regulation of adipocyte differentiation and function by polyunsaturated fatty acids
    Lise Madsen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
    Biochim Biophys Acta 1740:266-86. 2005
    ..Feeding status and hormonal background may therefore be of particular importance in determining the physiological effects of PUFAs of the n-6 family...
  32. ncbi A high-capacity assay for PPARgamma ligand regulation of endogenous aP2 expression in 3T3-L1 cells
    G Marie Thompson
    Department of Metabolic Disorders, Merck Research Laboratories, 126 E Lincoln Ave, Rahway, NJ 07065, USA
    Anal Biochem 330:21-8. 2004
    ..Since the assay obviates the need for RNA isolation and is performed in an automatable multiwell format, it can serve as a high-throughput, cell-based screen for the identification and characterization of PPARgamma modulators...
  33. ncbi Sortilin is essential and sufficient for the formation of Glut4 storage vesicles in 3T3-L1 adipocytes
    Jun Shi
    Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Dev Cell 9:99-108. 2005
    ..Thus, sortilin is not only essential, but also sufficient for biogenesis of GSVs and acquisition of insulin responsiveness in adipose cells...
  34. ncbi The transcription factor AP-2beta causes cell enlargement and insulin resistance in 3T3-L1 adipocytes
    Yukari Tao
    Division of Endocrinology and Metabolism, Department of Medicine, Shiga University of Medical Science, Seta, Otsu, Japan
    Endocrinology 147:1685-96. 2006
    ..We propose that AP-2beta is a candidate gene for producing adipocyte hypertrophy and may relate to the abnormal characteristics of adipocytes observed in obesity...
  35. ncbi Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity
    Utpal B Pajvani
    Department of Cell Biology, Division of Endocrinology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Biol Chem 279:12152-62. 2004
    ..These observations suggest that the HMW adiponectin complex is the active form of this protein, which we directly demonstrate in vivo by its ability to depress serum glucose levels in a dose-dependent manner...
  36. ncbi The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species
    Ying Lin
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:4617-26. 2005
    ..Combined, these results highlight the importance ROS production in adipocytes and the associated insulin resistance and inflammatory response...
  37. ncbi Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome
    Lydia E Kuo
    Department of Physiology and Biophysics, Georgetown University Medical Center, 3900 Reservoir Rd NW, BSB 234, Washington, DC 20057, USA
    Nat Med 13:803-11. 2007
    ..Thus, manipulations of NPY2R activity within fat tissue offer new ways to remodel fat and treat obesity and metabolic syndrome...
  38. ncbi A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization
    Libin Liu
    Department of Biochemistry, Boston University Medical School, Boston, Massachusetts 02118, USA
    J Biol Chem 283:4314-22. 2008
    ..We propose that the presence of cavin on the inside surface of caveolae stabilizes these structures, probably through interaction with the cytoskeleton, and cavin therefore plays an important role in caveolae formation and organization...
  39. pmc The neck of caveolae is a distinct plasma membrane subdomain that concentrates insulin receptors in 3T3-L1 adipocytes
    Michelangelo Foti
    Department of Cellular Physiology and Metabolism, Faculty of Medicine, University of Geneva, CH 1211 Geneva, Switzerland
    Proc Natl Acad Sci U S A 104:1242-7. 2007
    ....
  40. pmc Insulin-modulated Akt subcellular localization determines Akt isoform-specific signaling
    Eva Gonzalez
    Department of Biochemistry, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:7004-9. 2009
    ..Together, our findings reveal the stimulus-induced subcellular compartmentalization of Akt kinases as a mechanism contributing to specify Akt isoform functions...
  41. pmc Cell surface heparan sulfate proteoglycans contribute to intracellular lipid accumulation in adipocytes
    Larissa C Wilsie
    Department of Biochemistry and Molecular Biology, University of New Mexico, Health Sciences Center, 915 Camino de Salud, Albuquerque, New Mexico 87131, USA
    Lipids Health Dis 4:2. 2005
    ....
  42. ncbi Hypoxia is a potential risk factor for chronic inflammation and adiponectin reduction in adipose tissue of ob/ob and dietary obese mice
    Jianping Ye
    Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808, USA
    Am J Physiol Endocrinol Metab 293:E1118-28. 2007
    ..These data suggest a potential role of hypoxia in the induction of chronic inflammation and inhibition of adiponectin in the adipose tissue in obesity...
  43. ncbi Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes
    P Christopher LaRosa
    Center for Biotechnology, University of Nebraska, Lincoln, Nebraska 68588 0665, USA
    Physiol Genomics 31:544-53. 2007
    ..The release of proinflammatory cytokines may explain how the ISR in early stage adipocytes causes lipid loss in mature adipocytes...
  44. ncbi A paracrine loop between adipocytes and macrophages aggravates inflammatory changes: role of free fatty acids and tumor necrosis factor alpha
    Takayoshi Suganami
    Department of Molecular Medicine and Metabolism, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 101 0062, Japan
    Arterioscler Thromb Vasc Biol 25:2062-8. 2005
    ..Here we developed an in vitro coculture system composed of adipocytes and macrophages and examined the molecular mechanism whereby these cells communicate...
  45. pmc Adiponectin and leptin are secreted through distinct trafficking pathways in adipocytes
    Linglin Xie
    Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    Biochim Biophys Acta 1782:99-108. 2008
    ..Taken together, these results suggest that leptin and adiponectin secretion involve distinct intracellular compartments and that endosomal compartments are required for adiponectin but not for leptin secretion...
  46. pmc Direct quantification of fusion rate reveals a distal role for AS160 in insulin-stimulated fusion of GLUT4 storage vesicles
    Li Jiang
    Joint Laboratory of Institute of Biophysics and Huazhong University of Science and Technology, National Laboratory of Biomacromolecules, Chinese Academy of Sciences, Beijing, China
    J Biol Chem 283:8508-16. 2008
    ....
  47. ncbi Wnt4 and Wnt5a promote adipocyte differentiation
    Makoto Nishizuka
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Nagoya, Aichi 467 8603, Japan
    FEBS Lett 582:3201-5. 2008
    ..Furthermore, the inhibition of Wnt4 or Wnt5a expression prevented the accumulation of triacylglycerol and decreased the expression of adipogenesis-related genes. Wnt4 and Wnt5a have crucial roles in adipogenesis as positive regulators...
  48. ncbi A Nuclear Receptor Atlas: 3T3-L1 adipogenesis
    Mingui Fu
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 6001 Forest Park Road, Dallas, Texas 75390 9050, USA
    Mol Endocrinol 19:2437-50. 2005
    ....
  49. ncbi NF-kappaB is involved in the TNF-alpha induced inhibition of the differentiation of 3T3-L1 cells by reducing PPARgamma expression
    Gi Nam Chae
    Department of Biochemistry, College of Medicine, Dankook University, San 29 Anseo Dong, Cheonan 330 714, Korea
    Exp Mol Med 35:431-7. 2003
    ..These results suggest that inhibition of 3T3-L1 cells differentiation by TNF-alpha is partly implemented through NF-kappaB and one of its downstream effectors be PPARgamma...
  50. pmc Identification of amino acid residues within the C terminus of the Glut4 glucose transporter that are essential for insulin-stimulated redistribution to the plasma membrane
    Xiao Mei Song
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:12571-85. 2008
    ..We conclude that residues within the IRM are critical for the targeting of Glut4, but not of IRAP, to insulin-responsive intracellular membrane compartments in 3T3-L1 adipocytes...
  51. ncbi CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes
    Sebastien Vankoningsloo
    Laboratory of Biochemistry and Cellular Biology, University of Namur F U N D P, rue de Bruxelles, 61, 5000 Namur, Belgium
    J Cell Sci 119:1266-82. 2006
    ..These results highlight a new role for CREB in the control of triglyceride metabolism during the adaptative response of preadipocytes to mitochondrial dysfunction...
  52. ncbi The luminal Vps10p domain of sortilin plays the predominant role in targeting to insulin-responsive Glut4-containing vesicles
    Jun Shi
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 282:9008-16. 2007
    ..Our results suggest that luminal interactions between component proteins play an important role in the process of IRV biogenesis...
  53. pmc GLUT4 is sorted to vesicles whose accumulation beneath and insertion into the plasma membrane are differentially regulated by insulin and selectively affected by insulin resistance
    Wenyong Xiong
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10065, USA
    Mol Biol Cell 21:1375-86. 2010
    ..Differences in insulin sensitivities of various processes may be a general mechanism for the development of the physiologically important phenomenon of selective insulin resistance...
  54. pmc Cidea is associated with lipid droplets and insulin sensitivity in humans
    Vishwajeet Puri
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 105:7833-8. 2008
    ..The data support a model whereby failure of this pathway results in ectopic lipid accumulation, insulin resistance, and its associated comorbidities in humans...
  55. ncbi Suppressor of cytokine signaling 3 is a physiological regulator of adipocyte insulin signaling
    Hang Shi
    Division of Endocrinology, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA
    J Biol Chem 279:34733-40. 2004
    ..We conclude that SOCS3 plays an important role in mediating insulin resistance and may be an excellent target for therapeutic intervention in insulin resistance and type II diabetes...
  56. ncbi Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells
    Yan Nan Shen
    Department of Biology, Kyung Hee University, Seoul, Korea
    BMB Rep 42:691-6. 2009
    ..Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes...
  57. ncbi Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes
    Dawn L Brasaemle
    Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    J Biol Chem 279:46835-42. 2004
    ....
  58. ncbi Overexpression of micro ribonucleic acid 29, highly up-regulated in diabetic rats, leads to insulin resistance in 3T3-L1 adipocytes
    Aibin He
    The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, The Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China
    Mol Endocrinol 21:2785-94. 2007
    ..Taken together, these data reveal the crucial role of miR-29 in type 2 diabetes...
  59. ncbi Rab10, a target of the AS160 Rab GAP, is required for insulin-stimulated translocation of GLUT4 to the adipocyte plasma membrane
    Hiroyuki Sano
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Cell Metab 5:293-303. 2007
    ....
  60. pmc An RNA interference-based screen identifies MAP4K4/NIK as a negative regulator of PPARgamma, adipogenesis, and insulin-responsive hexose transport
    Xiaoqing Tang
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 103:2087-92. 2006
    ..These results reveal a MAP4K4/NIK-dependent signaling pathway that potently inhibits PPARgamma-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport...
  61. ncbi Temporal recruitment of CCAAT/enhancer-binding proteins to early and late adipogenic promoters in vivo
    N Salma
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    J Mol Endocrinol 36:139-51. 2006
    ..We have concluded that C/EBP family members bind to adipogenic genes in vivo in a manner that follows the induction of C/EBP protein synthesis...
  62. ncbi Mest/Peg1 imprinted gene enlarges adipocytes and is a marker of adipocyte size
    Mayumi Takahashi
    Division of Clinical Nutrition, National Institute of Health and Nutrition, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    Am J Physiol Endocrinol Metab 288:E117-24. 2005
    ..Moreover, adipocytes were markedly enlarged in the transgenic mice. Thus Mest appears to enlarge adipocytes and could be a novel marker of the size of adipocytes...
  63. pmc Insulin-stimulated plasma membrane fusion of Glut4 glucose transporter-containing vesicles is regulated by phospholipase D1
    Ping Huang
    Department of Pharmacology, University Medical Center at Stony Brook, Stony Brook, NY 11794 5140, USA
    Mol Biol Cell 16:2614-23. 2005
    ....
  64. ncbi Visfatin is released from 3T3-L1 adipocytes via a non-classical pathway
    Masaki Tanaka
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka, Japan
    Biochem Biophys Res Commun 359:194-201. 2007
    ..In addition, visfatin was not released on microvesicles. These results suggest that visfatin should be released from 3T3-L1 adipocytes via an ER-Golgi or microvesicles independent pathway...
  65. pmc Transcriptional regulation of adipogenesis by KLF4
    Kivanc Birsoy
    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10021, USA
    Cell Metab 7:339-47. 2008
    ..KLF4 is specifically induced in response to cAMP, which by itself can partially activate adipogenesis. These data suggest that KLF4 functions as an immediate early regulator of adipogenesis to induce C/EBPbeta...
  66. ncbi Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy
    Xiaolin Nan
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    J Lipid Res 44:2202-8. 2003
    ..CARS microscopy, in tandem with other techniques, provides exciting possibilities for studying LD dynamics under physiological conditions without perturbation of cell functions...
  67. ncbi Krüppel-like factor-6 promotes preadipocyte differentiation through histone deacetylase 3-dependent repression of DLK1
    Dan Li
    Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 280:26941-52. 2005
    ....
  68. ncbi Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms
    Zhu Chen
    Laboratory of Molecular Genetics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Cell Metab 1:93-106. 2005
    ..These data indicate that Krox20 is necessary for adipogenesis and that, when overexpressed, Krox20 potently stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms...
  69. ncbi Green tea polyphenol epigallocatechin gallate inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes
    Ji Lin
    444 Edgar L Rhodes Center for Animal and Dairy Science, University of Georgia, Athens, GA 30602 2771, USA
    Obes Res 13:982-90. 2005
    ..The objective of this study was to determine whether epigallocatechin gallate (EGCG), the primary green tea catechin, could act directly on adipocytes to inhibit adipogenesis and induce apoptosis...
  70. pmc Identification of a distal GLUT4 trafficking event controlled by actin polymerization
    Jamie A Lopez
    Diabetes and Obesity Research Program, The Garvan Institute of Medical Research, Sydney, NSW 2010, Australia
    Mol Biol Cell 20:3918-29. 2009
    ..These data clearly resolve multiple steps of the final stages of GLUT4 trafficking, demonstrating a crucial role for actin in the final stage of this process...
  71. pmc Inhibitor of DNA binding 2 is a small molecule-inducible modulator of peroxisome proliferator-activated receptor-gamma expression and adipocyte differentiation
    Kye Won Park
    Howard Hughes Medical Institute, University of California, Los Angeles, California 90095, USA
    Mol Endocrinol 22:2038-48. 2008
    ..These results outline a role for Id2 in the modulation of PPARgamma expression and adipogenesis and underscore the utility of adipogenic small molecules as tools to dissect adipocyte biology...
  72. pmc Uncoupling of 3T3-L1 gene expression from lipid accumulation during adipogenesis
    Karla A Temple
    Department of Medicine and Committee on Molecular Metabolism and Nutrition, The University of Chicago, Chicago, IL 60637, USA
    FEBS Lett 581:469-74. 2007
    ..These data indicate that extracellular energy is required for induction of adipocytic proteins, while only glucose sustained the parallel increase in triglyceride storage...
  73. ncbi Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4
    Sandra Lobo
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Lipid Res 48:609-20. 2007
    ..These findings define unique and distinct roles for FATP1 and FATP4 in adipose fatty acid metabolism...
  74. ncbi Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes
    Eun Jae Cho
    Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712 714, Korea
    J Microbiol Biotechnol 18:80-7. 2008
    ..Our results suggest a role for CO as anti-obesity agents by inhibiting adipocyte differentiation mediated through the downregulated expression of adipogenic transcription factors and other specific genes...
  75. ncbi Upregulated expression of stanniocalcin-1 during adipogenesis
    Martina Serlachius
    Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
    Exp Cell Res 296:256-64. 2004
    ..Given this, our findings suggest that STC-1, also in terminally differentiated adipocytes, may function as a "survival factor", which contributes to the maintenance of the integrity of mature adipose tissue...
  76. ncbi Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats
    Yuka Ono
    Matsuura Yakugyo Co Ltd, Enjo cho, Showa Ku, Nagoya 459 8001, Japan
    J Ethnopharmacol 106:238-44. 2006
    ..Therefore, NNE impaired digestion, inhibited absorption of lipids and carbohydrates, accelerated lipid metabolism and up-regulated energy expenditure. Consequently, NNE is beneficial for the suppression of obesity...
  77. ncbi Effects of mitochondrial uncoupling on adipocyte intracellular Ca(2+) and lipid metabolism
    Xiaocun Sun
    University of Tennessee, Knoxville, TN 37996, USA
    J Nutr Biochem 14:219-26. 2003
    ....
  78. ncbi Identification of dicarboxylate carrier Slc25a10 as malate transporter in de novo fatty acid synthesis
    Shinji Mizuarai
    Tsukuba Research Institute, Banyu Pharmaceutical Co Ltd, Ibaraki, Japan
    J Biol Chem 280:32434-41. 2005
    ....
  79. pmc Insulin stimulates membrane fusion and GLUT4 accumulation in clathrin coats on adipocyte plasma membranes
    Shaohui Huang
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Mol Cell Biol 27:3456-69. 2007
    ....
  80. pmc Repression of GLUT4 expression by the endoplasmic reticulum stress response in 3T3-L1 adipocytes
    Ryan S Miller
    Department of Pediatrics, Johns Hopkins University School of Medicine, 200 North Wolfe Street, Baltimore, MD 21287, USA
    Biochem Biophys Res Commun 362:188-92. 2007
    ..Our studies identify repression of GLUT4 expression as another potential mechanism for obesity-induced activation of the ER stress response to contribute to the insulin resistance of obesity...
  81. ncbi Stearoyl-CoA desaturase 2 is required for peroxisome proliferator-activated receptor gamma expression and adipogenesis in cultured 3T3-L1 cells
    Jennifer L Christianson
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    J Biol Chem 283:2906-16. 2008
    ..These results reveal that SCD2 is required for the induction and maintenance of PPARgamma protein levels and adipogenesis in 3T3-L1 cells...
  82. pmc ETO/MTG8 is an inhibitor of C/EBPbeta activity and a regulator of early adipogenesis
    Justin J Rochford
    Department of Clinical Biochemistry, University of Cambridge, Box 232, Level 4, Addenbrooke s Hospital, Hills Rd, Cambridge CB2 2QR, United Kingdom
    Mol Cell Biol 24:9863-72. 2004
    ..These findings define, for the first time, a molecular role for ETO in normal physiology as an inhibitor of C/EBPbeta and a novel regulator of early adipogenesis...
  83. pmc GLUT4 is internalized by a cholesterol-dependent nystatin-sensitive mechanism inhibited by insulin
    Vincent Blot
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    EMBO J 25:5648-58. 2006
    ..Our work thus identifies cholesterol-dependent uptake as a novel target of insulin action in adipocytes...
  84. ncbi Regulation of adipocyte differentiation and insulin action with rapamycin
    Hye Jin Cho
    School of Biological Sciences, Seoul National University, Seoul 151 742, Republic of Korea
    Biochem Biophys Res Commun 321:942-8. 2004
    ....
  85. ncbi JNK and tumor necrosis factor-alpha mediate free fatty acid-induced insulin resistance in 3T3-L1 adipocytes
    M T Audrey Nguyen
    Division of Endocrinology Metabolism, University of California, San Diego, La Jolla, California 92093 0673, USA
    J Biol Chem 280:35361-71. 2005
    ....
  86. pmc Intracellular ceramide synthesis and protein kinase Czeta activation play an essential role in palmitate-induced insulin resistance in rat L6 skeletal muscle cells
    Darren J Powell
    Division of Molecular Physiology, Faculty of Life Sciences, MSI WTB complex, University of Dundee, Dundee, DD1 5EH, Scotland
    Biochem J 382:619-29. 2004
    ..These findings indicate that intracellular ceramide synthesis and PKCzeta activation are important aspects of the mechanism by which palmitate desensitizes L6 muscle cells to insulin...
  87. ncbi The mitogenic and antiapoptotic actions of ghrelin in 3T3-L1 adipocytes
    Min Seon Kim
    Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon Dong, Chongno Gu, Seoul 110 744, Korea
    Mol Endocrinol 18:2291-301. 2004
    ..These effects may be mediated via activation of the MAPK and phosphatidylinositol 3-kinase/Akt pathways...
  88. ncbi Wnt signaling inhibits adipogenesis through beta-catenin-dependent and -independent mechanisms
    Jennifer A Kennell
    Program in Cellular and Molecular Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0622, USA
    J Biol Chem 280:24004-10. 2005
    ..These data supported a model in which Wnt signaling inhibits adipogenesis through both beta-catenin-dependent and beta-catenin-independent mechanisms...
  89. pmc Evidence of a possible role for Lys-gamma3-MSH in the regulation of adipocyte function
    Stephen C Harmer
    School of Biological Sciences, The University of Reading, Whiteknights, PO Box 228, Reading, Berkshire RG6 6AJ, UK
    J Endocrinol 196:149-58. 2008
    ....
  90. ncbi Cholesterol-induced caveolin targeting to lipid droplets in adipocytes: a role for caveolar endocytosis
    Soazig Le Lay
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Traffic 7:549-61. 2006
    ..The present data identify lipid droplets as potential target organelles for caveolar endocytosis and demonstrate a role for caveolin-1 in the maintenance of free cholesterol levels in adipocyte lipid droplets...
  91. ncbi Regulation of insulin receptor substrate 1 pleckstrin homology domain by protein kinase C: role of serine 24 phosphorylation
    Ranmali Nawaratne
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, UK
    Mol Endocrinol 20:1838-52. 2006
    ..Hence we provide evidence that IRS1-PH domain function is important for normal insulin signaling and is regulated by serine phosphorylation in a manner that could contribute to insulin resistance...
  92. ncbi The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes
    Muriel Mari
    INSERM U568, 06107 Nice, France
    J Cell Sci 119:1297-306. 2006
    ..These observations suggest that the uncoupling between Rabip4 and Rab4 induces the insertion of Glut 4 molecules that are unable to transport glucose into the plasma membrane...
  93. ncbi Necdin and E2F4 are modulated by rosiglitazone therapy in diabetic human adipose and muscle tissue
    Allison B Goldfine
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Diabetes 55:640-50. 2006
    ..These data suggest that changes in necdin and E2F4 expression after rosiglitazone exposure in humans are associated with altered adipocyte differentiation and may contribute to improved insulin sensitivity in humans treated with TZDs...
  94. ncbi Lentiviral short hairpin ribonucleic acid-mediated knockdown of GLUT4 in 3T3-L1 adipocytes
    Wei Liao
    Department of Medicine, Division of Endocrinology and Metabolism, University of California, San Diego, La Jolla, 92093, USA
    Endocrinology 147:2245-52. 2006
    ..These studies indicate that lentiviral shRNA constructs provide an excellent approach to deliver functional siRNAs into 3T3-L1 adipocytes for studying insulin signaling and adipocyte biology...
  95. pmc Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins
    Carey N Lumeng
    Life Sciences Institute, 210 Washtenaw Ave, Ann Arbor, MI 48109, USA
    Am J Physiol Endocrinol Metab 292:E166-74. 2007
    ..Thus both indirect/secreted and direct/cell contact-mediated factors derived from macrophages influence insulin sensitivity in adipocytes...
  96. ncbi Characterization of ScAP-23, a new cell line from murine subcutaneous adipose tissue, identifies genes for the molecular definition of preadipocytes
    Ji Young Kim
    Department of Biochemistry and Cancer Biology, University of Toledo Health Science Campus, Toledo, Ohio 43614, USA
    Physiol Genomics 31:328-42. 2007
    ..This suggests that comparative gene expression studies of ScAP-23 and 3T3-L1 adipogenesis may be particularly fruitful in identifying preadipocyte-expressed genes that play a role in adipose tissue physiology and/or pathophysiology...
  97. ncbi Inhibition of the anti-adipogenic Hedgehog signaling pathway by cyclopamine does not trigger adipocyte differentiation
    W Cousin
    Institute of Signaling, Developmental Biology and Cancer CNRS UMR6543, Universite de Nice Sophia Antipolis, Stem Cells and Differentiation, Nice, France
    Biochem Biophys Res Commun 349:799-803. 2006
    ..This suggests that Hedgehog signaling is an inadequate pharmacological target for patient suffering from syndromes associated with a decrease in fat mass, such as the ones observed in lipodystrophies...
  98. pmc Orphan nuclear receptor TR2, a mediator of preadipocyte proliferation, is differentially regulated by RA through exchange of coactivator PCAF with corepressor RIP140 on a platform molecule GRIP1
    Pawan Gupta
    Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Nucleic Acids Res 35:2269-82. 2007
    ..This results in a biphasic effect of RA on the expression of TR2 in undifferentiated and differentiated cells, which is required for RA-stimulated preadipocyte proliferation...
  99. pmc Akt- and Foxo1-interacting WD-repeat-FYVE protein promotes adipogenesis
    Thorsten Fritzius
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    EMBO J 27:1399-410. 2008
    ..Thus, ProF modulates Foxo1 phosphorylation by Akt, promoting adipocyte differentiation. Furthermore, ProF might be involved in metabolic disorders such as diabetes...
  100. ncbi Preadipocyte response and impairment of differentiation in an inflammatory environment
    Odile Poulain-Godefroy
    CNRS 8090, Institute of Biology, Pasteur Institute, 1 rue Calmette, BP447, 59021 Lille, France
    Biochem Biophys Res Commun 356:662-7. 2007
    ..This suggests that toll-like receptor mediated activation could regulate maintenance of preadipocyte status, and inflammatory environment encountered in inflamed white adipose tissue...
  101. ncbi Palmitate modulates intracellular signaling, induces endoplasmic reticulum stress, and causes apoptosis in mouse 3T3-L1 and rat primary preadipocytes
    Wen Guo
    Dept of Medicine, Boston Univ School of Medicine, 670 Albany St, 207, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 293:E576-86. 2007
    ....

Research Grants62

  1. Biochemistry and Cell Biology of CHC22 Clathrin
    Frances M Brodsky; Fiscal Year: 2013
    ..The proposed research will characterize the molecular mechanism of CHC22 function, its cellular regulation and its relationship to membrane traffic pathways that malfunction in diabetes. ..
  2. The Role of MTP in Lipid Droplet Formation in Adipocytes
    Larry L Swift; Fiscal Year: 2013
    ....
  3. Requirement for Glucocorticoids for Adipogenesis in vivo.
    Charles A Harris; Fiscal Year: 2012
    ..These Aims should answer the question as to whether glucocorticoid ligand and receptor are required for adipogenesis in vivo. ..
  4. The role of nicotinamide N-methyltransferase in glucose and lipid homeostasis
    Pavlos Pissios; Fiscal Year: 2013
    ..This proposal has direct relevance to the NIDDK mission and will establish NNMT as a potential new candidate gene for the treatment of the metabolic syndrome. ..
  5. ADIPOCYTE PROTEIN SECRETION AND INSULIN ACTION
    Harvey Lodish; Fiscal Year: 2007
    ..Functional studies and knock-out mice will be generated for each of these secreted proteins as warranted. ..
  6. Androgen Receptor Function in White Adipocyte Development
    Bin He; Fiscal Year: 2010
    ..Knowledge in this field may well facilitate the development of adipocyte selective androgen receptor modulators, which may be a useful treatment strategy for obesity-associated metabolic diseases. ..
  7. Role of TUG in the Formation of Insulin-Responsive GLUT4 Vesicles
    Charisse Orme; Fiscal Year: 2011
    ..It is anticipated that the results of this work will have importance for understanding how GLUT4 glucose transporters are properly sorted from internal membranes into the insulin responsive compartment. ..
  8. Novel Mechanisms of PPARg in Adipose Tissue Development
    Yaacov Barak; Fiscal Year: 2012
    ..These insights will provide broad rationales for prevention and treatment of obesity. ..
  9. PHOSPHOENOLPYYRUVATE CARBOXYKINASE IN ADIPOSE TISSUE
    David Loose; Fiscal Year: 1991
    ..These factors will be purified using a classical biochemical approach. The long term objective of this research is to broaden our understanding of how hormones elicit differential and tissue specific responses...
  10. REGULATION OF LDL RECEPTORS IN ADIPOCYTES
    FREDRIC KRAEMER; Fiscal Year: 1993
    ....
  11. Protease Inhibitor Related Adipogenesis in HIV Infection
    Krishna Agrawal; Fiscal Year: 2003
    ..These studies will delineate the molecular mechanisms that may be responsible for the adipogenic side effects induced by the PIs in the presence of HIV infection. ..
  12. FATTY ACID ALPHA OXIDATION IN ADIPOCYTES
    Xiong Su; Fiscal Year: 2010
    ..Our study will gain significant insights into a novel pathway of fatty acid metabolism and the etiology of obesity and diabetes and will aid in the discovery of novel targets for treatment of these metabolic diseases. ..
  13. The role of calcineurin in adipocyte biology
    Neil Clipstone; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  14. REGULATION OF ENERGY BALANCE--INTESTINAL MECHANISMS
    Richard Atkinson; Fiscal Year: 1999
    ..Understanding the mechanisms of weight loss with obesity surgery may allow development of better medical and/or surgical procedures to treat obesity. ..
  15. INSULIN RECEPTOR/KINASE REGULATION IN THE INTACT CELLS
    Ronald Kohanski; Fiscal Year: 1991
    ....
  16. THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
    Michael Freemark; Fiscal Year: 2007
    ..The results of our studies should provide new insist into the roles of pituitary and placental hormones in adipose development and function. ..
  17. Insulin/TZD regulation of protein structure in fat cells
    Fred Schaufele; Fiscal Year: 2004
    ..conformation of C/EBPa, PPARg, RXRa and the same co-activators, and3. conformations, dimers and interactions of the above factors specifically in the neighborhood of the genes that they regulate. ..
  18. Role of corepressors in the adipocyte
    Ronald Cohen; Fiscal Year: 2006
    ..It is anticipated that an examination of corepressor action on adipocyte function will allow for a greater understanding of the balance of forces regulating adipocyte action and the development of obesity and insulin resistance. ..
  19. Adipose Metabolic Profiling for Obesity Drug Targeting
    Kyongbum Lee; Fiscal Year: 2005
    ..abstract_text> ..
  20. GENETIC CONTROL OF MAMMALIAN CELL SURFACE FUNCTIONS
    Nobuyoshi Shimizu; Fiscal Year: 1993
    ....
  21. INSULIN/IGF-I REGULATION OF ADIPOCYTE DEVELOPMENT
    Charles Rubin; Fiscal Year: 1991
    ..6) IGF-I and insulin-binding studies will be performed to correlate the level of occupancy of specific IGF-I and insulin receptors with the degree and extent of regulation of specific genes...
  22. Role of Beta-Catenin Antagonist Chibby in Adipogenesis
    Ken Ichi Takemaru; Fiscal Year: 2010
    ..Our research offers hope that blocking Cby's activity may be an effective therapy for obesity and its associated disorders. ..
  23. INTRACELLULAR SIGNALING IN REPLICATING PREADIPOCYTES
    JOYCE HARP; Fiscal Year: 2002
    ..We propose to investigate the role of the JAK-STAT pathway and the cytoskeletal-adhesion complex in preadipocyte replication. ..
  24. REGULATION OF ADIPOGENESIS BY RETINOBLASTOMA PROTEINS
    ROBERT MCGEHEE; Fiscal Year: 2001
    ..Based on these findings, they hypothesize that the retinoblastoma family of proteins is involved in adipocyte differentiation. The application proposes experiments designed to examine mechanisms through which these interactions occur. ..
  25. The Role of DGAT1 In Muscle Lipotoxicity
    Yi Hao Yu; Fiscal Year: 2006
    ..As a result, we may be able to identify new molecular target(s) for future drug therapy aimed at reducing lipotoxicity ..
  26. Contribution of ATGL in skeletal muscle to lipid metabolism and insulin action
    ERIN KERSHAW; Fiscal Year: 2008
    ..Evaluating the role of ATGL-mediated fat breakdown in skeletal muscle will provide important insights into the pathophysiology and treatment of obesity, insulin resistance, and related metabolic disorders. [unreadable] [unreadable]..
  27. LIPID METABOLISM IN FAT CELLS
    Wen Guo; Fiscal Year: 2010
    ..Together, these studies will provide new insights into the stress-induced regulation of lipogenesis in adipocytes, a potentially important avenue for modulating energy balance. ..
  28. Mechanisms of Energy Balance in the Adipocyte
    Richard Bradley; Fiscal Year: 2005
    ..abstract_text> ..
  29. DGAT Overexpression In Mouse Adipose Tissue and Muscle
    Yi Hao Yu; Fiscal Year: 2006
    ....
  30. Convergence of Nuclear Receptor and Forkhead Signaling
    Paul Dowell; Fiscal Year: 2004
    ..The excellent research environment at Johns Hopkins coupled with an extensive collaborative team of experts in relevant fields of study is ideal for successful completion of the proposed work. ..
  31. C/EBP alpha in Aging Liver
    Nikolai Timchenko; Fiscal Year: 2009
    ..Based on data obtained in this project, we are planning to start developing a strategy to correct liver proliferation in elderly. ..
  32. BIOCHEMICAL CHARACTERIZATION OF PPAR
    JOHN VANDEN HEUVEL; Fiscal Year: 2008
    ..Taken together the proposed studies will greatly enhance our knowledge of PPARalpha's mechanism of action and hence our understanding of an important class of carcinogen. ..
  33. Matrix Metalloproteinases, Exercise, and Muscle Damage
    David Allen; Fiscal Year: 2008
    ..Finally, subjects will be genotyped for known functional MMP promoter polymorphisms, which will be correlated with MMP levels and muscle damage and soreness following eccentric exercise. ..
  34. CHROMIUM ENHANCES INSULIN & GLUT4 ACTION VIA LIPID RAFTS
    JEFFREY ELMENDORF; Fiscal Year: 2008
    ..abstract_text> ..
  35. Triple threat screening for modifiers of Protein Ser/Thr Phosphatase 2C
    DAVID BRAUTIGAN; Fiscal Year: 2008
    ..Chemical modifiers of PP2C have potential medicinal applications and will be used as research tools to study signaling in normal and cancer cells. [unreadable] [unreadable] [unreadable]..
  36. Regulation of adipocyte differentiation and metabolism
    Ormond A MacDougald; Fiscal Year: 2010
    ....
  37. IL-15, Body Compositition and Insulin Sensivity in Aging
    LeBris S Quinn; Fiscal Year: 2010
    ..abstract_text> ..
  38. Role of Insulin Regulation of Beta Cell Function in Vivo and in Vitro
    Allison Goldfine; Fiscal Year: 2008
    ..These studies may provide a unifying explanation linking the combined defects in insulin secretion and action that underlie type 2 diabetes. ..
  39. Leukocyte C2GIcNAcT-I in atherosclerosis
    Yuqing Huo; Fiscal Year: 2009
    ..These studies will have significant implications for the inhibition of leukocyte C2GlcNAcT-l in the prevention and treatment of atherosclerosis and its complications. ..
  40. REGULATION OF LIPOGENESIS
    M Lane; Fiscal Year: 2001
    ..for derepression and repression of the SCD1 and SCD2 genes. ..
  41. Insulin Control of Fat Regulation and Exercise in Teens
    Kristen Nadeau; Fiscal Year: 2008
    ..The K-23 application will help me reach my long-term goal of being an independent investigator in the field of pediatric insulin resistance and type 2 diabetes. ..
  42. Lipid Droplets: Metabolic Consequences of Stored Neutral Lipids
    Dawn Brasaemle; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  43. Indentification of AKT2/aPKC Substrates in Adipocytes
    Prem Sharma; Fiscal Year: 2005
    ..We believe that the findings from the proposed investigations will improve our general understanding of players of insulin resistance and offer potential insight into new therapeutic modalities. ..
  44. Molecular Genetics of Deafness in the Ames Waltzer Model
    Kumar Alagramam; Fiscal Year: 2005
    ..Additionally, we also plan to identify proteins that interact with the protein encoded by Pcdh15. ..
  45. Antiadipogenic Influence of HIV Protease Inhibitors
    M Lane; Fiscal Year: 2005
    ..The long term objective of the proposed research is to facilitate the design of anti-HIV therapeutics that lack lipoatrophic potential. ..
  46. Analysis of Therapeutic Adipose Tissue Remodeling
    JAMES GRANNEMAN; Fiscal Year: 2004
    ..2) To examine adipose tissue cellular dynamics during therapeutic remodeling induced by agonist activation of beta3-adrenergic receptors and PPAR-gamma. ..
  47. APOE RECYCLING: Cell Biology and Physiologic Relevance
    LARRY SWIFT; Fiscal Year: 2004
    ..These studies will primarily focus on apoE recycling by the hepatocyte, but will also expand to the macrophage, a cell type for which the impact of apoE recycling on function may be of great relevance to atherogenesis. ..
  48. IDENTIFICATION AND STUDY OF PPARgamma-REGULATED GENES
    DOROTHY SEARS; Fiscal Year: 2004
    ..abstract_text> ..
  49. PHYSIOLOGY AND MOLECULAR DETERMINANTS OF DIABETES
    Allison Goldfine; Fiscal Year: 2004
    ....
  50. C/EBPB And Mitotic Clonal Expansion During Adipogenesis
    Qi Qun Tang; Fiscal Year: 2004
    ..identify the kinase(s) that catalyzes this/these phosphorylation/s and to determine how the kinase(s) is regulated. -determine the role of C/EBP-beta in mitotic clonal expansion and how phosphorylation of C/EBP-beta affects this role...
  51. Chemokines, nitric oxide, and myocardial depression
    Bysani Chandrasekar; Fiscal Year: 2005
    ..The results from these studies will further our knowledge of chemokine biology and lead to new therapeutic strategies to prevent or treat myocardial dysfunction in ischemia-reperfusion injury. ..
  52. Gene Therapy of Muscle Wasting From Uremia
    Xiaonan Wang; Fiscal Year: 2005
    ..We believe that the experimental results will shed the insight on the mechanisms of muscle protein degradation, and provide a possible new approach to treat the pathological process of muscle wasting. ..
  53. Genistein induces thymic atrophy: a health concern?
    Paul Cooke; Fiscal Year: 2006
    ..These studies will advance our understanding of isoflavone effects on the immune system, and also may have relevance for soy-fed infants and laboratory rodents consuming soy-based feed. ..
  54. Inhibition of leukocyte C2GlcNAcT--l reduces thrombosis
    Yuqing Huo; Fiscal Year: 2007
    ..Inhibition of C2GIcNAcT-I may be a novel approach for preventive and therapeutic interventions in arterial thrombosis in atherosclerosis. ..
  55. RAPAMYCIN BLOCKADE OF PROTEIN PHOSPHATASE SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2007
    ..The proposed studies will give new insights into an under-studied phosphatase-signaling pathway and provide information on the molecular basis for action of and resistance to rapamycin. ..
  56. HORMONAL CONTROL OF ADIPOSE GENE EXPRESSION
    M Lane; Fiscal Year: 2007
    ..The 32kDa CUP/AP-2alpha- interacting protein, p32, previously identified in our lab, will be characterized and its role determined. ..
  57. Mechanism of Promotion of Adipogenesis by Adenovirus-36
    NIKHIL DHURANDHAR; Fiscal Year: 2007
    ..We believe that determining the role of viral infections in human obesity may influence the treatment, management, and possible prevention of such type of obesity. [unreadable] [unreadable]..
  58. PPARGamma in Sepsis-Induced Immunosuppression
    Raju Reddy; Fiscal Year: 2006
    ..abstract_text> ..
  59. FACTORS AND GENES RESPONSIBLE FOR ADIPOCYTE COMMITMENT
    M Lane; Fiscal Year: 2006
    ..abstract_text> ..
  60. CHROMIUM ENHANCEMENT OF INSULIN SIGNALING
    DAVID BRAUTIGAN; Fiscal Year: 2006
    ..abstract_text> ..
  61. Developmental toxicity of environmental chemicals
    FREDERICK VOM SAAL; Fiscal Year: 2006
    ..5a-reductase activity will be determined by radiometric assay. ..
  62. ROLE OF INSULIN AND IGF-1 ON BROWN ADIPOCYTES
    Yu Hua Tseng; Fiscal Year: 2003
    ..Accomplishment of the proposed studies will further our understanding on hormonal controls of metabolism and survival of brown adipocytes. ..