- Characterization of hybrid cells derived from spontaneous fusion events between breast epithelial cells exhibiting stem-like characteristics and breast cancer cells
Institute of Immunology, Zentrum für Biomedizinische Ausbildung und Forschung an der UWH ZBAF, Witten Herdecke University, Stockumer Str 10, 58448 Witten, Germany
Clin Exp Metastasis 28:75-90. 2011
..In the present study we characterized hybrid cells derived from spontaneous fusion events between the breast epithelial cell line M13SV1-EGFP-Neo and two breast ..
- Terahertz radiation induces spindle disturbances in human-hamster hybrid cells
Institut fur Pharmakologie und Toxikologie, Universitat Wurzburg, Germany
Radiat Res 175:569-74. 2011
..The findings in the present study do not necessarily imply disease or injury but may be important for evaluating possible underlying mechanisms...
- Spontaneous formation of tumorigenic hybrids between breast cancer and multipotent stromal cells is a source of tumor heterogeneity
Nevada Cancer Institute, Las Vegas, NV, USA
Am J Pathol 180:2504-15. 2012
..Our findings reconcile the fusion theory of cancer progression with the common observation that breast cancer metastases are generally aneuploid, but not tetraploid, and are histopathologically similar to the primary neoplasm...
- Periodic activation of Wnt/beta-catenin signaling enhances somatic cell reprogramming mediated by cell fusion
Telethon Institute of Genetics and Medicine, Via P Castellino, 111, 80131 Naples, Italy
Cell Stem Cell 3:493-507. 2008
..Our data thus demonstrate that in ESCs, periodic beta-catenin accumulation via the Wnt/beta-catenin pathway provides a specific threshold that leads to the reprogramming of somatic cells after fusion...
- A new class of membrane-bound chemokine with a CX3C motif
J F Bazan
Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
Nature 385:640-4. 1997
- delta-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation
Institute of Pharmacology, Toxicology and Pharmacy Ludwig Maximilians University of Munich Koeniginstrasse 16, 80539 Muenchen, Federal Republic of Germany
Exp Cell Res 315:2115-25. 2009
..the signal transduction pathway by which opioids induce Akt activation in neuroblastomaxglioma (NG108-15) hybrid cells. Exposure of the cells to both [D-Pen(2,5)]enkephalin and etorphine resulted in a time- and dose-dependent ..
- Gene expression pattern in transmitochondrial cytoplasmic hybrid cells harboring type 2 diabetes-associated mitochondrial DNA haplogroups
Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
PLoS ONE 6:e22116. 2011
- Deciphering the molecular genetic basis of NPC through functional approaches
Hong Lok Lung
Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, Pokfulam, Hong Kong SAR, People s Republic of China
Semin Cancer Biol 22:87-95. 2012
..These studies show the advantages of using functional approaches for identification of TSGs...
- Cell fusion is a potent inducer of aneuploidy and drug resistance in tumor cell/ normal cell hybrids
Institute of Immunology, Witten Herdecke University, Stockumer Str 10, 58448 Witten, Germany
Crit Rev Oncog 18:97-113. 2013
..Particularly in the tumor context, cell fusion has been associated with a progression of this disease since hybrid cells derived from fusion events between tumor cells and normal cells, such as macrophages and adult stem cells, ..
- Horizontal transmission and retention of malignancy, as well as functional human genes, after spontaneous fusion of human glioblastoma and hamster host cells in vivo
David M Goldenberg
Garden State Cancer Center, Center for Molecular Medicine and Immunology, Morris Plains, NJ, USA
Int J Cancer 131:49-58. 2012
- Changing potency by spontaneous fusion
Qi Long Ying
Centre for Genome Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
Nature 416:545-8. 2002
..not arise by direct conversion of brain to embryonic stem cell but rather through spontaneous generation of hybrid cells. The tetraploid hybrids exhibit full pluripotent character, including multilineage contribution to chimaeras...
- Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells
Chad A Cowan
Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
Science 309:1369-73. 2005
..Human embryonic stem (hES) cells were fused with human fibroblasts, resulting in hybrid cells that maintain a stable tetraploid DNA content and have morphology, growth rate, and antigen expression patterns ..
- Cell fusion is the principal source of bone-marrow-derived hepatocytes
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA
Nature 422:897-901. 2003
..We conclude that hepatocytes derived form bone marrow arise from cell fusion and not by differentiation of haematopoietic stem cells...
- Pluripotential reprogramming of the somatic genome in hybrid cells occurs with the first cell cycle
Dong Wook Han
Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, Germany
Stem Cells 26:445-54. 2008
- mtDNA G10398A variant in African-American women with breast cancer provides resistance to apoptosis and promotes metastasis in mice
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
J Hum Genet 54:647-54. 2009
..Together our studies suggest that the G10398A variant confers resistance to apoptosis and promotes metastasis...
- Human monocyte x mouse melanoma fusion hybrids express human gene
A K Chakraborty
Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520, USA
Gene 275:103-6. 2001
..This observation may lead us to find contributory genes from monocyte and/or macrophage that are responsible for modulating the genotypes and hence the phenotypes in the hybrids...
- Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells
Precursory Research for Embryonic Science and Technology (PRESTO, JST, Kawaguchi-shi, Japan
Curr Biol 11:1553-8. 2001
..The pluripotency of the ES-thymocyte hybrid cells is shown in vivo, since they contribute to all three primary germ layers of chimeric embryos...
- Cybrid models of Parkinson's disease show variable mitochondrial biogenesis and genotype-respiration relationships
Paula M Keeney
Morris K Udall Parkinson s Research Center of Excellence, University of Virginia, PO Box 800394, Charlottesville, VA 22908, USA
Exp Neurol 220:374-82. 2009
..Cybrids remain a valuable model for some aspects of sPD but their heterogeneity mitigates against a simple designation of sPD phenotype in this cell model...
- Erasure of cellular memory by fusion with pluripotent cells
Jeong Tae Do
Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, Germany
Stem Cells 25:1013-20. 2007
..F9-NSC hybrid cells displayed various features of reprogramming, such as reactivation of pluripotency genes, inactivation of tissue-..
- Pancreatic ductal cells acquire mesenchymal characteristics through cell fusion with bone marrow-derived mesenchymal stem cells and SIRT1 attenuates the apoptosis of hybrid cells
Pancreatic Disease Institute, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Cells Tissues Organs 196:129-36. 2012
..Cell fusion between somatic cells and bMSCs to form hybrid cells may have an important role in tissue repair through the subsequent reprogramming of the somatic cell nucleus...
- CD34 hybrid cells promote endothelial colony-forming cell bioactivity and therapeutic potential for ischemic diseases
Jun Hee Lee
Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, Korea
Arterioscler Thromb Vasc Biol 33:1622-34. 2013
..Although endothelial progenitor cells (EPCs) have been reported to promote neovessel formation during vascular injury, the function of supporting cells of EPCs and their interaction with EPCs during EPC isolation remain unclear...
- Monochromosome transfer provides functional evidence for growth-suppressive genes on chromosome 14 in nasopharyngeal carcinoma
Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong Special Administrative Region, China
Genes Chromosomes Cancer 37:359-68. 2003
- Analysis of aberrant methylation of the VHL gene by transgenes, monochromosome transfer, and cell fusion
Intramural Research Support Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland, MD 21702, USA
Oncogene 18:5672-9. 1999
..Contribution of some putative trans-acting factors cannot be excluded during a period when the aberrant VHL methylation pattern was first generated...
- Spontaneous hybridization of macrophages and Meth A sarcoma cells
Lill Tove R Busund
Department of Clinical Pathology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
Int J Cancer 98:573-81. 2002
..The macrophage DNA index was set at 1; Meth A cells were found to have an index of 1.6 in G1 phase, and the hybrids had a 2.6 index. The hybrids grew more slowly in vitro than Meth A cells, but grew faster in vivo...
- Oligonucleotide microarray expression analysis of genes whose expression is correlated with tumorigenic and non-tumorigenic phenotype of HeLa x human fibroblast hybrid cells
Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697 4025, USA
Cancer Lett 165:201-9. 2001
..These findings suggest that synthetic oligonucleotide arrays are a powerful and highly reproducible tool to identify those genes whose expression is associated with certain phenotypes...
- Positive contribution of pathogenic mutations in the mitochondrial genome to the promotion of cancer by prevention from apoptosis
Department of Pathology, Tokyo Women s Medical University, School of Medicine, Tokyo, Japan
Cancer Res 65:1655-63. 2005
..Because apoptosis occurred less frequently in the mutant versus wild-type cybrids in cultures and tumors, the pathogenic mtDNA mutations seem to promote tumors by preventing apoptosis...
- Immunogenicity and therapeutic efficacy of dendritic-tumor hybrid cells generated by electrofusion
Center for Surgery Research, Cleveland Clinic Foundation, Ohio 44195, USA
Clin Immunol 104:14-20. 2002
..These results provide an impetus for treating cancer patients with similarly generated cells...
- Nucleosomes are translationally positioned on the active allele and rotationally positioned on the inactive allele of the HPRT promoter
Department of Biochemistry and Molecular Biology, Center for Mammalian Genetics, University of Florida, Gainesville, 32610, USA
Mol Cell Biol 21:7682-95. 2001
- Spontaneously formed tumorigenic hybrids of Meth A sarcoma and macrophages grow faster and are better vascularized than the parental tumor
Lill Tove R Busund
Department of Clinical Pathology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
Int J Cancer 100:407-13. 2002
- Targeted chromosome elimination from ES-somatic hybrid cells
Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
Nat Methods 4:23-5. 2007
..we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single ..
- Purified hybrid cells from dendritic cell and tumor cell fusions are superior activators of antitumor immunity
Oncology Research Institute, Greenville Hospital System, SC 29605, USA
Cancer Immunol Immunother 50:456-62. 2001
..Because of the difficulty of purifying the hybrid cells from the fusion, fusion mixtures were used in these studies...
- Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes
Barbara A Boggs
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
Nat Genet 30:73-6. 2002
- Transplanted bone marrow regenerates liver by cell fusion
Division of Hematology, University of Washington, Seattle, Washington 98195, USA
Nature 422:901-4. 2003
..We also show that the haematopoietic donor genome adopts a more hepatocyte-specific expression profile after cell fusion, as the wild-type Fah gene was activated and the pan-haematopoietic CD45 marker was no longer expressed...
- Mechanisms of the genotoxicity of crocidolite asbestos in mammalian cells: implication from mutation patterns induced by reactive oxygen species
Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
Environ Health Perspect 110:1003-8. 2002
..These results provide direct evidence that the mutagenicity of asbestos is mediated by ROS in mammalian cells...
- Tumour-dendritic hybrid cell vaccination for the treatment of patients with malignant melanoma: immunological effects and clinical results
Department of Dermatology and Allergy, Charite, University Medicine Berlin, Humboldt University, Schumannstr 20 21, 10117 Berlin, Germany
Vaccine 23:2367-73. 2005
..Eleven of fourteen patients, clinical responders and non-responders alike, mounted high-frequency T cell responses to various tumour-associated antigens. Failing clinical responses correlated with loss of antigenicity...
- Mutagenicity of arsenic in mammalian cells: role of reactive oxygen species
T K Hei
Center for Radiological Research, College of Physicians and Surgeons, School of Public Health, Columbia University, New York, NY 10032, USA
Proc Natl Acad Sci U S A 95:8103-7. 1998
..Thus, the carcinogenicity of arsenite can be explained at least in part by it being a mutagen that depends on reactive oxygen species for its activity...
- Enhancement of sodium current in NG108-15 cells during neural differentiation is mainly due to an increase in NaV1.7 expression
Department of Biological Chemistry, School of Pharmacy, Kinki University, Kowakae 3 4 1, Higasiosaka 577 8502, Japan
Neurochem Res 32:1469-75. 2007
..These results suggest that the enhancement of TTX-sensitive sodium current density in differentiated NG108-15 cells is mainly due to the increase in the expression of the TTX-sensitive voltage-gated Na+ channel, NaV1.7...
- Mitochondrial respiration and respiration-associated proteins in cell lines created through Parkinson's subject mitochondrial transfer
A Raquel Esteves
Centro de Neurociencias e Biologia Celular, Universidade de Coimbra, Coimbra, Portugal
J Neurochem 113:674-82. 2010
..We conclude mitochondrial respiration and pathways influenced by aerobic metabolism are altered in NT2 cybrid cell lines generated through transfer of PD subject platelet mitochondria...
- Phenotypic characteristics of hybrid cells produced by cell fusion of porcine adrenal chromaffin cells with human mesenchymal stem cells: a preliminary study
The Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, 1601 West Taylor Street, Chicago, IL 60612, USA
Neurol Res 30:217-22. 2008
- Hybrid cells formed by fusion of Epstein-Barr virus-associated B-lymphoblastoid cells and either marrow-derived or solid tumour-derived cell lines display different co-stimulatory phenotypes and abilities to activate allogeneic T-cell responses in vitro
A L Cywinski
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK
Tissue Antigens 68:115-26. 2006
..By contrast, hybrid cells derived from three solid tumours only expressed MHC class II when the parent tumour cell line expressed MHC ..
- Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T
MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
Gene 411:69-76. 2008
..We found that there were no functionally-important bioenergetic differences between mitochondria bearing these haplogroups, using either isolated mitochondria or mitochondria within cells...
- The cybrid model of sporadic Parkinson's disease
Patricia A Trimmer
Morris K Udall Parkinson s Disease Research Center of Excellence, Department of Neurology, University of Virginia, Charlottesville, 22908, USA
Exp Neurol 218:320-5. 2009
..sPD cybrid models can be improved by the use of non-tumor human stem cell-derived neural precursor cells and by an introduction of postmortem brain mtDNA to test its causality directly...
- Senescence of immortal human fibroblasts by the introduction of normal human chromosome 6
A K Sandhu
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark 07103
Proc Natl Acad Sci U S A 91:5498-502. 1994
..Based upon these results we propose that at least one of the genes (SEN6) for cellular senescence in human fibroblasts is present on the long arm of chromosome 6...
- Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines
MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
Biochem J 404:345-51. 2007
..Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups...
- Mitochondrial function in Parkinson's disease cybrids containing an nt2 neuron-like nuclear background
A Raquel F Esteves
Centro de Neurociencias e Biologia Celular, Universidade de Coimbra, Hospitais da Universidade de Coimbra, 3004 Coimbra, Portugal
Mitochondrion 8:219-28. 2008
..Equivalent MPP+ exposures are more likely to trigger programmed cell death in PD cybrid cells than in control cybrid cells. Our data support a relatively upstream role for mitochondrial dysfunction in idiopathic PD...
- Complementation of V(D)J recombination defect and X-ray sensitivity of scid mouse cells by human chromosome 8
S S Banga
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103 2757
Mutat Res 315:239-47. 1994
..These observations demonstrate that the human homolog of the mouse scid gene resides on human chromosome 8...
- Hybrids of dendritic cells and tumor cells generated by electrofusion simultaneously present immunodominant epitopes from multiple human tumor-associated antigens in the context of MHC class I and class II molecules
Maria R Parkhurst
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 170:5317-25. 2003
b>Hybrid cells generated by fusing dendritic cells with tumor cells (DC-TC) are currently being evaluated as cancer vaccines in preclinical models and human immunization trials...
- Relationships among molecular genetic and respiratory properties of Parkinson's disease cybrid cells show similarities to Parkinson's brain tissues
M Kathleen Borland
Center for the Study of Neurodegenerative Diseases, University of Virginia, Charlottesville, Virginia, USA
Biochim Biophys Acta 1792:68-74. 2009
..This will require characterization of the abnormalities and dynamics of mtDNA changes propagated through sPD cybrids over time...
- Effective treatment of spontaneous metastases derived from a poorly immunogenic murine mammary carcinoma by combined dendritic-tumor hybrid vaccination and adoptive transfer of sensitized T cells
Center for Surgery Research NE62, Department of Immunology, Cleveland Clinic, Cleveland, 9500 Euclid Ave, Cleveland, OH 44195, USA
Clin Immunol 127:66-77. 2008
..Taken together, the combined approach may be useful for clinical development of cancer immunotherapy...
- Identification of highly expressed genes in metastasis-suppressed chromosome 6/human malignant melanoma hybrid cells using subtractive hybridization and differential display
J H Lee
The Jake Gittlen Cancer Research Institute, Pennsylvania State University College of Medicine, Hershey 17033 0850, USA
Int J Cancer 71:1035-44. 1997
..9, following transfection and constitutive expression. Our results illustrate the power of subtractive hybridization and differential display to identify functional metastasis-controlling genes in human melanoma...
- The effectiveness of 20 mev protons at nanosecond pulse lengths in producing chromosome aberrations in human-hamster hybrid cells
T E Schmid
Klinikum rechts der Isar, Department of Radiation Oncology, Technische Universitat Munchen, D 81675 Munchen, Germany
Radiat Res 175:719-27. 2011
- Generation of highly pure fusions of colorectal carcinoma and antigen-presenting cells
Division of Molecular Oncology and Immunotherapy, Department of General Surgery, University of Rostock, Schillingallee 35, 18055, Rostock, Germany
Langenbecks Arch Surg 395:365-71. 2010
..One approach is the fusion of antigen-presenting cells (APCs) with tumor cells. Hybrid cells combine the antigenicity of tumors with the immunostimulatory capacity of APCs...
- Coexistence of alternative lengthening of telomeres and telomerase in hTERT-transfected GM847 cells
Cancer Research Group, Children's Medical Research Institute, Westmead, Sydney, New South Wales 2145, Australia
Mol Cell Biol 21:3862-75. 2001
..These hybrid cells were telomerase positive, and the telomeres decreased in length, very rapidly at first and then at the rate ..
- Vaccination of dendritic cells loaded with interleukin-12-secreting cancer cells augments in vivo antitumor immunity: characteristics of syngeneic and allogeneic antigen-presenting cell cancer hybrid cells
Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan
Clin Cancer Res 11:58-66. 2005
..This novel IL-12-producing fusion cell vaccine might be one promising intervention for future cancer immunotherapy...
- A chemical enucleation method for the transfer of mitochondrial DNA to rho(o) cells
Maria Pilar Bayona-Bafaluy
Department of Neurology, University of Miami School of Medicine, Miami, FL 33136, USA
Nucleic Acids Res 31:e98. 2003
..This method greatly facilitates the production of transmitochondrial cybrids, thereby increasing the number of mtDNA mutations and the recipient cell types that can be studied by this system...
- A higher resolution radiation hybrid map of bovine chromosome 13
Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne, Bremgartenstrasse 109 A, 3012 Berne, Switzerland
Genet Sel Evol 34:255-67. 2002
..The present map increases the marker density and the marker resolution on BTA13 and enables further insight into the evolutionary development of the chromosome as compared to man...
- Correction of a nucleotide-excision-repair mutation by human chromosome 19 in hamster-human hybrid cells
L H Thompson
Somat Cell Mol Genet 11:87-92. 1985
..This study demonstrates the mapping of a human repair gene by direct selection of complementing hybrids in the presence of a DNA-damaging agent (mitomycin C)...
- Semiallogeneic cancer vaccines formulated with granulocyte-macrophage colony-stimulating factor for patients with metastatic gastrointestinal adenocarcinomas: a pilot phase I study
D A Newton
Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, USA
J Immunother 24:19-26. 2001
..Because of the simplicity, feasibility, and flexibility of this immunotherapeutic approach, semiallogeneic hybrid vaccines have the potential to be used in the treatment of virtually any type of cancer...
- The human X-inactivation centre is not required for maintenance of X-chromosome inactivation
C J Brown
Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106
Nature 368:154-6. 1994
..Genes subject to X inactivation remain transcriptionally silent despite the loss of the XIC, demonstrating that the presence of the XIC is not required for the maintenance of X inactivation in somatic cells...
- Improvements in the use of neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins
IRTA, Ctra Poble Nou, km 5 5, 43540 Sant Carles de la Rapita, Tarragona, Spain
Toxicon 55:381-9. 2010
..Evaporation time of toxin solutions before cell exposure could be an important source of variability in AZA-1 toxic response evaluation...
- Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
Proc Natl Acad Sci U S A 93:13925-30. 1996
..Although < 2% of the DNA in the hybrid cells was human, as much as 80% of transformants had human DNA YACs when a TAR cloning vector contained Alu repeats...
- Variability of X chromosome inactivation: effect on levels of TIMP1 RNA and role of DNA methylation
Catherine L Anderson
Department of Medical Genetics, University of British Columbia, Vancouver, Canada
Hum Genet 110:271-8. 2002
..However, as women age, they may accumulate cells stably expressing TIMP1 from the inactive X, with a resulting increase of TIMP1, which may explain some sex differences in various late-onset disorders...
- Myeloma cell-induced disruption of bone remodelling compartments leads to osteolytic lesions and generation of osteoclast-myeloma hybrid cells
Thomas L Andersen
Department of Clinical Cell Biology, Vejle Hospital, CSFU IRS, University of Southern Denmark
Br J Haematol 148:551-61. 2010
..increased bone resorption, and fusion of myeloma cells with osteoclasts thereby forming myeloma-osteoclast hybrid cells. These findings strongly support a critical role of BRC canopies in myeloma-induced bone disease...
- A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation
International Institute of Genetics and Biophysics, CNR, Naples, Italy
Nat Genet 13:227-9. 1996
..Unlike all Xp PAR genes studied so far, a synaptobrevin-like gene, tentatively named SYBL1, undergoes X inactivation. In addition, it is also inactive on the Y chromosome, thereby maintaining dosage compensation in an unprecedented way...
- Investigation of bystander effects in hybrid cells by means of cell fusion and premature chromosome condensation induction
G I Terzoudi
Institute of Radioisotopes and Radiodiagnostic Products, National Centre for Scientific Research Demokritos, Athens, Greece
Radiat Res 173:789-801. 2010
..These observations and the methodology developed should be useful in the further development of protocols for investigating bystander responses and for elucidating the underlying mechanisms...
- Large scale mapping of methylcytosines in CTCF-binding sites in the human H19 promoter and aberrant hypomethylation in human bladder cancer
Department of Biochemistry and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA
Hum Mol Genet 10:2619-26. 2001
..Loss of methylation imprinting of H19 is linked to hypomethylation of the paternal allele in human bladder cancer, unlike the situation in Wilms' tumor and colon cancer where the maternal allele becomes hypermethylated...
- Characteristics of hybrid cells obtained by dendritic cell/tumour cell fusion in a T-47D breast cancer cell line model indicate their potential as anti-tumour vaccines
EA3499, Universite Paris 6, Laboratoire d Histologie Biologie Tumorale, Hopital Tenon, AP HP, Paris, France
Int J Oncol 31:1357-65. 2007
..identified using confocal microscopy to avoid an overestimation of fusion efficiency and to distinguish clearly hybrid cells from aggregated or phagocytosed cells...
- The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells
S F Dowdy
Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine 92717
Nucleic Acids Res 19:5763-9. 1991
..The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype...
- Chromosomal autonomy of hMLH1 methylation in colon cancer
Department of Microbiology and Molecular Biology, Case Western Reserve University, Cleveland, Ohio, OH 44106, USA
Oncogene 21:1443-9. 2002
..We posit that hMLH1 methylation is dependent on and maintained by cis chromosomal marks, whose nature remains to be elucidated...
- Calretinin and calbindin D-28k, but not parvalbumin protect against glutamate-induced delayed excitotoxicity in transfected N18-RE 105 neuroblastoma-retina hybrid cells
Institute of Histology and General Embryology, University of Fribourg, CH 1705 Fribourg, Switzerland
Brain Res 945:181-90. 2002
..We have stably transfected N18-RE 105 neuroblastoma-retina hybrid cells with the cDNAs for the three CaBPs and investigated the effect of these proteins on the L-glutamate-induced, Ca(..
- Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in a neuroblastoma patient. Establishment of somatic cell hybrids and identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on chromosome 17 as breakpoint flanking single copy markers
Department of Pediatric Oncology, University Hospital, Ghent, Belgium
Oncogene 10:1087-93. 1995
..As a pivotal step towards breakpoint cloning, at present these flanking markers optimally delineate the breakpoint regions of both chromosomes 1 and 17 at the molecular level...
- Mutagenic effects of a single and an exact number of alpha particles in mammalian cells
T K Hei
Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
Proc Natl Acad Sci U S A 94:3765-70. 1997
..These data provide direct evidence that a single a particle traversing a nucleus will have a high probability of resulting in a mutation and highlight the need for radiation protection at low doses...
- Spontaneous fusion of cells between species yields transdifferentiation and retroviral transfer in vivo
Brenda M Ogle
Transplantation Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
FASEB J 18:548-50. 2004
..These hybrid cells divide, express human and porcine proteins, and contribute to porcine nonhematopoietic tissues...
- Stabilization and localization of Xist RNA are controlled by separate mechanisms and are not sufficient for X inactivation
C M Clemson
Department of Cell Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01655, USA
J Cell Biol 142:13-23. 1998
..XIST RNA does not localize in reactivants or in naturally inactive human X chromosomes in mouse/ human hybrid cells. The XIST transcripts are fully stabilized despite their inability to localize, and hence XIST RNA localization ..
- Histone code modifications on pluripotential nuclei of reprogrammed somatic cells
Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Mol Cell Biol 24:5710-20. 2004
..Here, we show that the reprogrammed somatic genome of ES hybrid cells becomes hyperacetylated at H3 and H4, while lysine 4 (K4) of H3 becomes globally hyper-di- and -tri-methylated...
- Human chromosome 17 comparative anchor loci are conserved on bovine chromosome 19
Y P Yang
Department of Veterinary Pathobiology, Texas A and M University, College Station 77843, USA
Genomics 27:293-7. 1995
- Mitochondrial damage mediates genotoxicity of arsenic in mammalian cells
Su Xian Liu
Center for Radiological Research, Department of Neurology, Columbia University, 630 West 168th Street, New York, NY 10032, USA
Cancer Res 65:3236-42. 2005
- Differential gene expression in tumorigenic and nontumorigenic HeLa x normal human fibroblast hybrid cells
Department of Microbiology and Molecular Genetics, University of California Irvine College of Medicine, Irvine, California, USA
Mol Carcinog 26:298-304. 1999
..in the control of tumorigenic expression, we performed differential display screening of nontumorigenic hybrid cells and tumorigenic segregants...
- Comparative study of the use of neuroblastoma cells (Neuro-2a) and neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins
Centre d Aqüicultura, IRTA, Ctra Poble Nou s n Km 5 5, 43540 Sant Carles de la Rapita, Tarragona, Spain
Toxicon 52:541-50. 2008
..Both cell types were able to identify Na-channel acting toxins and allowed to quantify the effect of saxitoxin, brevetoxin-3, palytoxin, okadaic acid, dinophysistoxin-1 and pectenotoxin-2 under different experimental conditions...
- Characterization of the toxic mechanism triggered by Alzheimer's amyloid-beta peptides via p75 neurotrophin receptor in neuronal hybrid cells
Departments of Pharmacology and Anatomy, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
J Neurosci Res 73:627-36. 2003
..Here we report that Abeta causes cell death in neuronal hybrid cells transfected with p75NTR, but not in nontransfected cells, and that p75NTR(L401K) cannot mediate Abeta ..
- RHO--radiation hybrid ordering
Department of Computer Science, Technion, Haifa 32000, Israel
Genome Res 10:365-78. 2000
..For three chromosomes, the results differ significantly (lod score >1.75), with chromosome 2 having the largest improvement (lod score 3.776)...
- Reprogramming in inter-species embryonal carcinoma-somatic cell hybrids induces expression of pluripotency and differentiation markers
Intercytex Ltd, Manchester, United Kingdom
Cloning Stem Cells 5:339-54. 2003
..Colonies of hybrid cells displayed heterogeneity in cellular morphology as well as in their pattern of human gene expression...
- Regeneration of somatic hybrids in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica
School of Life Sciences, Shandong University, Jinan 250100, Shandong, China
Genome 47:680-8. 2004
..These results indicate that the regeneration of hybrid plants involves not only the integration of S. italica nuclear and organellar DNA, but also the genome complementation of T1 and T2...
- LIT1, an imprinted antisense RNA in the human KvLQT1 locus identified by screening for differentially expressed transcripts using monochromosomal hybrids
Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Nishimachi, Yonago, Japan
Hum Mol Genet 8:1209-17. 1999
..Thus, this positional approach using human monochromosomal hybrids could contribute to the efficient identification of imprinted loci in humans...
- X inactivation analysis and DNA methylation studies of the ubiquitin activating enzyme E1 and PCTAIRE-1 genes in human and mouse
Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
Hum Mol Genet 5:391-401. 1996
..Methylation of CpG residues at restriction sites at the 5' end of both genes on the murine inactive X chromosome is consistent with both genes being subject to X inactivation in mouse, in contrast to their expression status in humans...
- Direct coupling of opioid receptors to both stimulatory and inhibitory guanine nucleotide-binding proteins in F-11 neuroblastoma-sensory neuron hybrid cells
R A Cruciani
Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461
Proc Natl Acad Sci U S A 90:3019-23. 1993
..to the inhibitory and "other" G proteins, gi and Go, in F-11 (neuroblastoma-dorsal root ganglion neuron) hybrid cells. Treatment of intact F-11 cells with cholera toxin decreased specific binding of the opioid agonist [D-Ala2,D-..
- Mutagenicity of diesel exhaust particles mediated by cell-particle interaction in mammalian cells
Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Hefei, PR China
Toxicology 229:91-100. 2007
..05). These results provided direct evidence that DEPs was mutagenic in mammalian cells and that cell-particle interaction played an essential role in the process...
- Chromosomal localization of a human band 3-like gene to region 7q35----7q36
A P Palumbo
Am J Hum Genet 39:307-16. 1986
..In situ hybridization analysis using the c-DNA for this nonerythroid Band 3 gene further localized the gene to region 7q35----7q36 of human metaphase chromosomes...
- Assignment of the aquaporin-8 water channel gene (AQP8) to human chromosome 16p12
Istituto di Genetica, Universita degli Studi di Bari, Italy
Cytogenet Cell Genet 84:208-10. 1999
- Reactivation of an inactive human X chromosome introduced into mouse embryonal carcinoma cells by microcell fusion with persistent expression of XIST
Research Center for Molecular Genetics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan
Exp Cell Res 230:208-19. 1997
..The level of XIST expression in our hybrid cells was almost identical to that of parental CF150 cells...
- Extinction of albumin gene expression in a panel of human chromosome 2 microcell hybrids
K M Cerosaletti
Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
Genomics 31:348-58. 1996
..These data define and map a genetic locus on human chromosome 2 that extinguishes albumin gene expression in trans, and they suggest that TSE2-mediated extinction is independent of HNF-1, -4, -3 alpha, and -3 beta expression...
- Polymorphic X-chromosome inactivation of the human TIMP1 gene
C L Anderson
Department of Medical Genetics, University of British Columbia, Vancouver, Canada
Am J Hum Genet 65:699-708. 1999
..However, in other cells, no expression of TIMP1 was observed from the inactive X chromosome, suggesting that TIMP1 inactivation is polymorphic in human females...
- Generation of hybrid cell lines with endothelial potential from spontaneous fusion of adult bone marrow cells with embryonic fibroblast feeder
National University Medical Institute, National University of Singapore, Singapore
In Vitro Cell Dev Biol Anim 40:143-9. 2004
- Human embryonic stem cells reprogram myeloid precursors following cell-cell fusion
Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53706, USA
Stem Cells 24:168-76. 2006
..When the hybrid cells were formed into embryoid bodies, upregulation of genes characteristic of the three germ layers and ..
- Vaccination therapy with tumor-dendritic cell hybrids: a promising therapeutic approach?
Holger A Haenssle
Georg August University of Goettingen, Department of Dermatology, von Siebold Str 3, D 37075 Goettingen, Germany
Curr Opin Investig Drugs 6:1240-5. 2005
..As an alternative to loading DCs with tumor antigen preparations, hybrid cells generated by fusing tumor cells with DCs have successfully been evaluated in preclinical cancer models...
- Activation of antitumor cytotoxic T lymphocytes by fusion of patient-derived dendritic cells with autologous osteosarcoma
Center of Orthopedic Surgery Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi an 710038, Shaanxi, China
Exp Oncol 27:273-8. 2005
..This study was designed to investigate the antitumor effects of tumor vaccine produced by electrofusion between human osteosarcoma cells and DCs...
- Fusion hybrid of dendritic cells and engineered tumor cells expressing interleukin-12 induces type 1 immune responses against tumor
Research Unit, Saskatchewan Cancer Agency, Department of Oncology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Tumori 91:531-8. 2005
..In the present study, we investigated the antitumor immunity derived from vaccination of fusion hybrids between DCs and engineered J558/IL-12 myeloma cells secreting Th1 cytokine IL-12...
- Hybrid-primed lymphocytes and hybrid vaccination prevent tumor growth of lewis lung carcinoma in mice
Department of Hematology and Oncology, Medical Center University of Giessen, Giessen, Germany
J Immunother 29:175-87. 2006
..b>Hybrid cells (HCs) were used to explore 3 potential tumor therapy strategies: protective immunization, vaccination, and ..
- [Establishment of dendritomas by fusion of human dendritic cells with human hepatocellular carcinoma cell line HLE cells]
Department of Hepatobiliary Surgery, Peking University People s Hospital, Beijing 100044, China
Zhonghua Zhong Liu Za Zhi 27:465-7. 2005
..To construct dendritomas by fusion of human dendritic cells with HLE cells, a human hepatocellular carcinoma cell line...
- Changes in replication, nuclear location, and expression of the Igh locus after fusion of a pre-B cell line with a T cell line
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
J Immunol 175:2317-20. 2005
..These data are consistent with the silencing of a pre-B cell-specific replication program in the fusion hybrid cells and independent regulation of the nuclear location of Igh loci.
- [Immunotherapeutic reactivity of dendritic cells loaded with a variety of antigen preparations]
Dept of Surgery, Division of Digestive Surgery, Kyoto Prefectural University of Medicine
Gan To Kagaku Ryoho 31:1637-9. 2004
..In conclusion, DC-tumor fusion hybrids were the most effective vaccine to eradicate existing tumors. These data support the use of DC-tumor electrofusion cells for the treatment of human cancer...