spermatozoa

Summary

Summary: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.

Top Publications

  1. ncbi Epigenetic transgenerational actions of endocrine disruptors and male fertility
    Matthew D Anway
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4231, USA
    Science 308:1466-9. 2005
  2. pmc Distinctive chromatin in human sperm packages genes for embryo development
    Saher Sue Hammoud
    Howard Hughes Medical Institute, Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Nature 460:473-8. 2009
  3. ncbi Calcium channels in the development, maturation, and function of spermatozoa
    Alberto Darszon
    Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    Physiol Rev 91:1305-55. 2011
  4. pmc Transgenerational actions of environmental compounds on reproductive disease and identification of epigenetic biomarkers of ancestral exposures
    Mohan Manikkam
    Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, Washington, United States of America
    PLoS ONE 7:e31901. 2012
  5. pmc Sperm methylation profiles reveal features of epigenetic inheritance and evolution in primates
    Antoine Molaro
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 146:1029-41. 2011
  6. pmc PRDM9 variation strongly influences recombination hot-spot activity and meiotic instability in humans
    Ingrid L Berg
    Department of Genetics, University of Leicester, Leicester, UK
    Nat Genet 42:859-63. 2010
  7. pmc The CatSper channel: a polymodal chemosensor in human sperm
    Christoph Brenker
    Center of Advanced European Studies and Research caesar, Abteilung Molekulare Neurosensorik, Bonn, Germany
    EMBO J 31:1654-65. 2012
  8. pmc Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa
    Xu Hui Zeng
    Department of Anesthesiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 108:5879-84. 2011
  9. ncbi A sperm ion channel required for sperm motility and male fertility
    D Ren
    Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 413:603-9. 2001
  10. pmc A survey of small RNAs in human sperm
    Stephen A Krawetz
    Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
    Hum Reprod 26:3401-12. 2011

Detail Information

Publications373 found, 100 shown here

  1. ncbi Epigenetic transgenerational actions of endocrine disruptors and male fertility
    Matthew D Anway
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4231, USA
    Science 308:1466-9. 2005
    ..The ability of an environmental factor (for example, endocrine disruptor) to reprogram the germ line and to promote a transgenerational disease state has significant implications for evolutionary biology and disease etiology...
  2. pmc Distinctive chromatin in human sperm packages genes for embryo development
    Saher Sue Hammoud
    Howard Hughes Medical Institute, Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Nature 460:473-8. 2009
    ..Furthermore, developmental promoters are generally DNA hypomethylated in sperm, but acquire methylation during differentiation. Taken together, epigenetic marking in sperm is extensive, and correlated with developmental regulators...
  3. ncbi Calcium channels in the development, maturation, and function of spermatozoa
    Alberto Darszon
    Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    Physiol Rev 91:1305-55. 2011
    A proper dialogue between spermatozoa and the egg is essential for conception of a new individual in sexually reproducing animals. Ca(2+) is crucial in orchestrating this unique event leading to a new life...
  4. pmc Transgenerational actions of environmental compounds on reproductive disease and identification of epigenetic biomarkers of ancestral exposures
    Mohan Manikkam
    Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, Washington, United States of America
    PLoS ONE 7:e31901. 2012
    ..Exposure-specific epigenetic biomarkers were identified that may allow for the assessment of ancestral environmental exposures associated with adult onset disease...
  5. pmc Sperm methylation profiles reveal features of epigenetic inheritance and evolution in primates
    Antoine Molaro
    Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 146:1029-41. 2011
    ..Thus, the features that determine DNA methylation patterns differ between male germ cells and somatic cells, and elements of these features have diverged between humans and chimpanzees...
  6. pmc PRDM9 variation strongly influences recombination hot-spot activity and meiotic instability in humans
    Ingrid L Berg
    Department of Genetics, University of Leicester, Leicester, UK
    Nat Genet 42:859-63. 2010
    ....
  7. pmc The CatSper channel: a polymodal chemosensor in human sperm
    Christoph Brenker
    Center of Advanced European Studies and Research caesar, Abteilung Molekulare Neurosensorik, Bonn, Germany
    EMBO J 31:1654-65. 2012
    ..We propose that the CatSper channel complex serves as a polymodal sensor for multiple chemical cues that assist sperm during their voyage across the female genital tract...
  8. pmc Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa
    Xu Hui Zeng
    Department of Anesthesiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 108:5879-84. 2011
    Mouse spermatozoa express a pH-dependent K(+) current (KSper) thought to be composed of subunits encoded by the Slo3 gene...
  9. ncbi A sperm ion channel required for sperm motility and male fertility
    D Ren
    Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 413:603-9. 2001
    ..CatSper is thus vital to cAMP-mediated Ca2+ influx in sperm, sperm motility and fertilization. CatSper represents an excellent target for non-hormonal contraceptives for both men and women...
  10. pmc A survey of small RNAs in human sperm
    Stephen A Krawetz
    Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
    Hum Reprod 26:3401-12. 2011
    ..e. small non-coding) delivered from mammalian spermatozoa play a functional role in early embryo development...
  11. ncbi The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm
    Timo Strünker
    Center of Advanced European Studies and Research, Abteilung Molekulare Neurosensorik, Ludwig Erhard Allee 2, 53175 Bonn, Germany
    Nature 471:382-6. 2011
    ..The identification of CatSper channel blockers will greatly facilitate the study of Ca(2+) signalling in sperm and help to define further the physiological role of progesterone and CatSper...
  12. ncbi RNA-mediated non-mendelian inheritance of an epigenetic change in the mouse
    Minoo Rassoulzadegan
    INSERM, U636, F 06108 Nice, France
    Nature 441:469-74. 2006
    ..at the postmeiotic stages--at which time the gene is normally silent--leads to the accumulation of RNA in spermatozoa. Microinjection into fertilized eggs either of total RNA from Kit(tm1Alf/+) heterozygotes or of Kit-specific ..
  13. ncbi Mammalian recombination hot spots: properties, control and evolution
    Kenneth Paigen
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Nat Rev Genet 11:221-33. 2010
    ..This progress has been accompanied by the identification of trans-acting systems that regulate the location and relative activity of individual hot spots...
  14. ncbi Bax-deficient mice with lymphoid hyperplasia and male germ cell death
    C M Knudson
    Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 270:96-9. 1995
    ..Multinucleated giant cells and dysplastic cells accompanied massive cell death. Thus, the loss of Bax results in hyperplasia or hypoplasia, depending on the cellular context...
  15. ncbi Sperm morphology, motility, and concentration in fertile and infertile men
    D S Guzick
    Department of Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    N Engl J Med 345:1388-93. 2001
    ..Although semen analysis is routinely used to evaluate the male partner in infertile couples, sperm measurements that discriminate between fertile and infertile men are not well defined...
  16. ncbi Controls of germline stem cells, entry into meiosis, and the sperm/oocyte decision in Caenorhabditis elegans
    Judith Kimble
    Department of Biochemistry and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706 1544, USA
    Annu Rev Cell Dev Biol 23:405-33. 2007
    ..Parallels with controls in other organisms include the use of PUF proteins for stem cell maintenance and the prominence of mRNA regulation for the control of germline development...
  17. ncbi Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa
    Urszula Brykczynska
    Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
    Nat Struct Mol Biol 17:679-87. 2010
    ..and Polycomb-mediated gene regulation have methylation-specific distributions at regulatory regions in human spermatozoa. Histone H3 Lys4 dimethylation (H3K4me2) marks genes that are relevant in spermatogenesis and cellular ..
  18. ncbi Oxidative stress and male infertility--a clinical perspective
    Kelton Tremellen
    Repromed, 180 Fullarton Road, Dulwich, 5065 Adelaide, South Australia, Australia
    Hum Reprod Update 14:243-58. 2008
    ..Finally, the review will outline methods available for diagnosing oxidative stress and the various treatments available...
  19. ncbi Identification of a testicular odorant receptor mediating human sperm chemotaxis
    Marc Spehr
    Department of Cell Physiology, Ruhr University Bochum, 150 University Street, D 44780 Bochum, Germany
    Science 299:2054-8. 2003
    Although it has been known for some time that olfactory receptors (ORs) reside in spermatozoa, the function of these ORs is unknown...
  20. ncbi Sperm competition and ejaculate economics
    Geoff A Parker
    Division of Population and Evolutionary Biology, School of Biological Sciences, Crown Street, University of Liverpool, Liverpool, L69 7ZB, UK
    Biol Rev Camb Philos Soc 85:897-934. 2010
    ....
  21. ncbi Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel
    Yuriy Kirichok
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital and Harvard Medical School Enders 1309, Boston, Massachusetts 02115, USA
    Nature 439:737-40. 2006
    ..Here, by using a simple approach to patch-clamp spermatozoa and to characterize whole-spermatozoan currents, we describe a constitutively active flagellar calcium channel ..
  22. ncbi Male germ cell gene expression
    Edward M Eddy
    Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    Recent Prog Horm Res 57:103-28. 2002
    ..Nevertheless, an intrinsic evolutionarily conserved genetic program regulates male germ cell gene expression and development...
  23. pmc The SLO3 sperm-specific potassium channel plays a vital role in male fertility
    Celia M Santi
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO 63110, USA
    FEBS Lett 584:1041-6. 2010
    ..The failure of AR is rescued by valinomycin which hyperpolarizes mutant sperm. Thus SLO3 is the principal potassium channel responsible for capacitation-induced hyperpolarization, and membrane hyperpolarization is crucial to the AR...
  24. ncbi Capacitation status and in vitro fertility of boar spermatozoa: effects of seminal plasma, cumulus-oocyte-complexes-conditioned medium and hyaluronan
    K Suzuki
    Experimental Farm, Field Science Center FSC, Hokkaido University, Sapporo, Japan
    Int J Androl 25:84-93. 2002
    ..conditioned medium (CCM) and hyaluronan (HA) on functional changes and in vitro fertilizing ability of porcine spermatozoa were examined...
  25. pmc Disruption of the principal, progesterone-activated sperm Ca2+ channel in a CatSper2-deficient infertile patient
    James F Smith
    Department of Urology, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 110:6823-8. 2013
    ..Although it has been suggested that progesterone-dependent Ca(2+) influx into human spermatozoa is primarily mediated by cationic channel of sperm (CatSper), the principal flagellar Ca(2+) channel of sperm, ..
  26. pmc Ca2+ signals generated by CatSper and Ca2+ stores regulate different behaviors in human sperm
    Wardah Alasmari
    From the Reproductive and Developmental Biology, Medical School, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, Scotland, United Kingdom
    J Biol Chem 288:6248-58. 2013
    ....
  27. ncbi Sexual selection and sperm quantity: meta-analyses of strategic ejaculation
    Clint D Kelly
    Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, USA
    Biol Rev Camb Philos Soc 86:863-84. 2011
    ..Researchers should be explicit about which model they are testing to ensure that future meta-analyses can better partition studies into different categories, or control for continuous moderator variables...
  28. pmc Integrative DNA methylation and gene expression analyses identify DNA packaging and epigenetic regulatory genes associated with low motility sperm
    Sara E Pacheco
    Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island, United States of America
    PLoS ONE 6:e20280. 2011
    ..We performed a genome-wide analysis of sperm DNA methylation and mRNA content to test for associations with sperm function...
  29. ncbi Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
    Spermatogenesis is the process by which a single spermatogonium develops into 256 spermatozoa, one of which will fertilize the ovum...
  30. pmc Global mapping of DNA methylation in mouse promoters reveals epigenetic reprogramming of pluripotency genes
    Cassandra R Farthing
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, United Kingdom
    PLoS Genet 4:e1000116. 2008
    ..Epigenetic reprogramming in the germline prior to fertilisation and the reprogramming of key pluripotency genes in the early embryo is thus crucial for transmission of pluripotency...
  31. ncbi Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients
    Hisato Kobayashi
    Department of Obstetrics, Tohoku University Graduate School of Medicine, 2 1 Seiryo cho, Aoba ku, Sendai 980 8575, Japan
    Hum Mol Genet 16:2542-51. 2007
    ..Our data suggest that sperm from infertile patients, especially those with oligospermia, may carry a higher risk of transmitting incorrect primary imprints to their offspring, highlighting the need for more research into ART...
  32. ncbi Postmeiotic sex chromatin in the male germline of mice
    Satoshi H Namekawa
    Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Boston, 02114, USA
    Curr Biol 16:660-7. 2006
    ..We conclude that chromosome-wide X silencing continues from meiosis to the end of spermiogenesis, and we discuss implications for proposed mechanisms of imprinted X-inactivation...
  33. ncbi PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development
    Christopher M Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Development 129:3533-44. 2002
    ..Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo...
  34. pmc Sperm-borne microRNA-34c is required for the first cleavage division in mouse
    Wei Min Liu
    Department of Obstetrics and Gynaecology and Centre for Reproduction, Development and Growth, University of Hong Kong, Pokfulam, Hong Kong, People s Republic of China
    Proc Natl Acad Sci U S A 109:490-4. 2012
    ..Our findings provide evidence that sperm-borne miR-34c is important for the first cell division via modulation of Bcl-2 expression...
  35. pmc Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization
    Mayuko Jin
    Department of Biological Sciences, and Glycoscience Institute, Ochanomizu University, Tokyo 112 8610, Japan
    Proc Natl Acad Sci U S A 108:4892-6. 2011
    To fuse with oocytes, spermatozoa of eutherian mammals must pass through extracellular coats, the cumulus cell layer, and the zona pellucida (ZP)...
  36. ncbi Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics
    David Miller
    Division of Reproduction and Early Development, Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, Clarendon Way, Leeds LS2 9JT, UK
    Reproduction 139:287-301. 2010
    ..To achieve this remarkable level of compaction, spermatozoa replace most of their histones with smaller, highly basic arginine and (in eutherians) cysteine rich protamines...
  37. pmc Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes
    Shin Ichi Tomizawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Japan
    Development 138:811-20. 2011
    ..These results provide evidence for a unique methylation profile in oocytes and reveal the surprisingly dynamic nature of DMRs in the early embryo...
  38. ncbi Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis
    Denny Sakkas
    Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 333 Cedar St, New Haven, CT 06520, USA
    Fertil Steril 93:1027-36. 2010
    ....
  39. ncbi Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome
    M Bungum
    Fertility Clinic, Viborg Hospital, Skive, Denmark
    Hum Reprod 22:174-9. 2007
    ..The available data however, have been based on limited numbers of treatments. We aimed to define the clinical role of SCSA in assisted reproduction...
  40. ncbi Stimulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa
    Adam J Koppers
    Discipline of Biological Sciences and ARC Centre of Excellence in Biotechnology and Development, Faculty of Science and IT, University of Newcastle, Callaghan, NSW 2308, Australia
    Free Radic Biol Med 48:112-9. 2010
    ..etiology of this condition is thought to involve the excessive generation of reactive oxygen species by human spermatozoa; however, the cause of this aberrant activity is unknown...
  41. pmc Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrosomal reaction
    Gerardo Orta
    Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, CP 62210, Mexico
    J Physiol 590:2659-75. 2012
    Motility, maturation and the acrosome reaction (AR) are fundamental functions of mammalian spermatozoa. While travelling through the female reproductive tract, spermatozoa must mature through a process named capacitation, so that they ..
  42. ncbi Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel
    Polina V Lishko
    Department of Physiology, University of California, San Francisco, UCSF Mail Code 2140, Genentech Hall, Room N272F, 600 16 th Street, San Francisco, CA 94158, USA
    Cell 140:327-37. 2010
    Human spermatozoa are quiescent in the male reproductive system and must undergo activation once introduced into the female reproductive tract...
  43. ncbi Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor
    Y Chen
    Department of Pharmacology, Joan and Sanford I Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Science 289:625-8. 2000
    b>Spermatozoa undergo a poorly understood activation process induced by bicarbonate and mediated by cyclic adenosine 3',5'-monophosphate (cAMP)...
  44. ncbi The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs
    Naokazu Inoue
    Genome Information Research Center, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
    Nature 434:234-8. 2005
    ..Human sperm also contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs...
  45. pmc CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm
    Anne E Carlson
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 100:14864-8. 2003
    ..We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization...
  46. ncbi Evaluation of nuclear DNA damage in spermatozoa from infertile men with varicocele
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:1431-6. 2003
    ..To examine levels of sperm DNA damage and oxidative stress (OS) in infertile men with varicocele...
  47. pmc Sperm competition and the evolution of sperm design in mammals
    Maximiliano Tourmente
    Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales CSIC, Jose Gutierrez Abascal 2, 28006 Madrid, Spain
    BMC Evol Biol 11:12. 2011
    ..In addition, the debate has extended to which sperm components should increase in size: the midpiece to accommodate more mitochondria and produce more energy to fuel motility, or the principal piece to generate greater propulsion forces...
  48. ncbi Reciprocal crossover asymmetry and meiotic drive in a human recombination hot spot
    Alec J Jeffreys
    Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
    Nat Genet 31:267-71. 2002
    ..These findings provide an explanation for the relatively uniform widths of human crossover hot spots and suggest that hot spots may be generally prone to extinction by meiotic drive...
  49. ncbi Reproductive biology: delivering spermatozoan RNA to the oocyte
    G Charles Ostermeier
    Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan 48201, USA
    Nature 429:154. 2004
    ....
  50. pmc Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function
    Gerard M Gibbs
    Department of Anatomy and Developmental Biology, Monash University, VIC 3800, Australia
    Proc Natl Acad Sci U S A 108:7034-9. 2011
    ..Collectively, these data identify CRISP4 as an endogenous regulator of TRPM8 with a role in normal sperm function...
  51. pmc A network of interactions among seminal proteins underlies the long-term postmating response in Drosophila
    K Ravi Ram
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 106:15384-9. 2009
    ..The requirements for manifestation of the LTR in Drosophila establish the paradigm that multiple seminal proteins can exert their actions through a multistep, multicomponent network of interactions...
  52. ncbi Molecular identification of a hyperpolarization-activated channel in sea urchin sperm
    R Gauss
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich, Germany
    Nature 393:583-7. 1998
    ..Because of their sequence and functional properties, Ih channels form a class of their own within the superfamily of voltage-gated and cyclic-nucleotide-gated channels...
  53. pmc The epididymis, cytoplasmic droplets and male fertility
    Trevor G Cooper
    Centre of Reproductive Medicine and Andrology, University of Munster, Munster D 48149, Germany
    Asian J Androl 13:130-8. 2011
    The potential of spermatozoa to become motile during post-testicular maturation, and the relationship between the cytoplasmic droplet and fertilizing capacity are reviewed...
  54. pmc 3' UTRs are the primary regulators of gene expression in the C. elegans germline
    Christopher Merritt
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Curr Biol 18:1476-82. 2008
    ..Our findings indicate that many genes rely primarily on 3' UTRs, not promoters, for regulation during germline development...
  55. ncbi Male accessory gland secretions: modulators of female reproductive physiology and behavior
    Cedric Gillott
    Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK S7N 5E2, Canada
    Annu Rev Entomol 48:163-84. 2003
    ..In a few species the secretions include noxious chemicals. These are sequestered by developing eggs that are thereby protected from predators and pathogens when laid...
  56. ncbi The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse
    Irene Mendoza-Lujambio
    Institute of Human Genetics, Department of Zoology and Developmental Biology, University of Gottingen, 37073 Gottingen, Germany
    Hum Mol Genet 11:1647-58. 2002
    In mice carrying the autosomal recessive mutation 'abnormal spermatozoon head shape' (azh) all spermatozoa display a highly abnormal head morphology that differs drastically from the compact and hook-shaped head of the normal murine ..
  57. ncbi Lack of an association between environmental exposure to polychlorinated biphenyls and p,p'-DDE and DNA damage in human sperm measured using the neutral comet assay
    R Hauser
    Department of Environmental Health, Occupational Health Program, Harvard School of Public Health, Boston, MA 02115, USA
    Hum Reprod 18:2525-33. 2003
    ....
  58. pmc The control of male fertility by spermatozoan ion channels
    Polina V Lishko
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Annu Rev Physiol 74:453-75. 2012
    ..The uniqueness of sperm ion channels makes them ideal pharmaceutical targets for contraception. In this review we discuss how ion channels regulate sperm physiology...
  59. ncbi Characterization of the intracellular calcium store at the base of the sperm flagellum that regulates hyperactivated motility
    Han Chen Ho
    Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Biol Reprod 68:1590-6. 2003
    ..We concluded that the intracellular Ca2+ store is the part of RNE that contains enlarged cisternae and that Ca2+ is released directly to the axoneme to trigger hyperactivated motility without the active participation of mitochondria...
  60. ncbi Viable piglets generated from porcine oocytes matured in vitro and fertilized by intracytoplasmic sperm head injection
    Michiko Nakai
    Genetic Diversity Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8602, Japan
    Biol Reprod 68:1003-8. 2003
    ..by using porcine oocytes matured in vitro and fertilized by ICSI after evaluating the efficacy of using donor spermatozoa in which the acrosome had been artificially removed by treatment with calcium ionophore A23187 (Ca-I)...
  61. ncbi The Drosophila melanogaster sperm proteome-II (DmSP-II)
    Elizabeth R Wasbrough
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK
    J Proteomics 73:2171-85. 2010
    ..The use of whole-cell proteomics in conjunction with characterized phenotypes, functional annotations and pathway information has advanced our systems level understanding of sperm proteome functional networks...
  62. pmc TRY-5 is a sperm-activating protease in Caenorhabditis elegans seminal fluid
    Joseph R Smith
    Department of Human Genetics, University of Utah, Salt Lake City, Utah, USA
    PLoS Genet 7:e1002375. 2011
    ..In Caenorhabditis elegans, sperm differentiate from immature spermatids into mature, motile spermatozoa during a process termed sperm activation. For C...
  63. ncbi Oxidative stress & male infertility
    Kartikeya Makker
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obsterics Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Indian J Med Res 129:357-67. 2009
    ..It also highlights the mechanism of production and the pathophysiology of ROS in relation to the male reproductive system and enumerate the benefits of incorporating antioxidants in clinical and experimental settings...
  64. ncbi Sex-peptides: seminal peptides of the Drosophila male
    E Kubli
    Zoological Institute, University Zurich Irchel, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    Cell Mol Life Sci 60:1689-704. 2003
    ..Sps bind to sperm. Together with sperm they are responsible for the persistence of the two post-mating responses. But Sps are the molecular basis of the sperm effect; sperm is merely the carrier...
  65. ncbi Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients
    Paul Cohen-Bacrie
    Laboratoire d Eylau, Paris, France
    Fertil Steril 91:1801-5. 2009
    ....
  66. ncbi Resolving mechanisms of competitive fertilization success in Drosophila melanogaster
    Mollie K Manier
    Department of Biology, Syracuse University, Syracuse, NY 13244 1270, USA
    Science 328:354-7. 2010
    ..We find that competitive male fertilization success derives from a multivariate process involving ejaculate-female and ejaculate-ejaculate interactions, as well as complex sperm behavior in vivo...
  67. ncbi Male fertility in natural populations of red deer is determined by sperm velocity and the proportion of normal spermatozoa
    Aurelio F Malo
    Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales CSIC, 28006 Madrid, Spain
    Biol Reprod 72:822-9. 2005
    ..between sperm production and sperm swimming velocity, sperm motility and proportion of morphologically normal spermatozoa, and sperm viability and acrosome integrity...
  68. pmc Sperm proteomics reveals intensified selection on mouse sperm membrane and acrosome genes
    Steve Dorus
    Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
    Mol Biol Evol 27:1235-46. 2010
    b>Spermatozoa are a focal point for the impact of sexual selection due to sperm competition and sperm-female interactions in a wide range of sexually reproducing organisms...
  69. ncbi Oxidative stress, sperm survival and fertility control
    R John Aitken
    ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW 2308, Australia
    Mol Cell Endocrinol 250:66-9. 2006
    ..of redox cycling xenobiotics, infiltrating leukocytes and excess reactive oxygen species production by the spermatozoa. Whenever the levels of oxidative stress in the male germ line are high, the peroxidation of unsaturated fatty ..
  70. ncbi Transgenerational effects of the endocrine disruptor vinclozolin on the methylation pattern of imprinted genes in the mouse sperm
    Christelle Stouder
    Department of Genetic Medicine and Development Swiss Center for Applied Human Toxicology, University of Geneva Medical School, CMU, 1 Michel Servet, 1211 Geneva 4, Switzerland
    Reproduction 139:373-9. 2010
    ..The reported effects of EDCs on human male spermatogenesis might be mediated by analogous imprinting alterations...
  71. ncbi Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa
    Masafumi Ihara
    Biochemistry and Cell Biology Unit, HMRO, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Dev Cell 8:343-52. 2005
    ..In mouse and human spermatozoa, SEPT4 and other septins are found in the annulus, a cortical ring which separates the middle and principal ..
  72. ncbi Clinical relevance of oxidative stress in male factor infertility: an update
    Ashok Agarwal
    Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, OH 44195, USA
    Am J Reprod Immunol 59:2-11. 2008
    ..The benefits of incorporating antioxidants in clinical and experimental settings have been enumerated. We also highlight the emerging concept of utilizing OS as a method of contraception and the potential problems associated with it...
  73. ncbi Conserved role of nanos proteins in germ cell development
    Masayuki Tsuda
    Division of Mammalian Development, National Institute of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Science 301:1239-41. 2003
    ..Hence, although mice and flies differ in their mechanisms for germ cell specification, there seems to be conserved function for nanos proteins among invertebrates and vertebrates...
  74. pmc The sex peptide of Drosophila melanogaster: female post-mating responses analyzed by using RNA interference
    Tracey Chapman
    Department of Biology, Darwin Building, and Wolfson Institute for Biomedical Research, Cruciform Building, University College London, Gower Street, London WC1E 6BT, UK
    Proc Natl Acad Sci U S A 100:9923-8. 2003
    ..The prolonged "sperm effect" on female receptivity and egg production is therefore entirely attributable to SP, but sperm are necessary for its occurrence...
  75. ncbi Smoking induces differential miRNA expression in human spermatozoa: a potential transgenerational epigenetic concern?
    Emma L Marczylo
    Systems Toxicology Group, MRC Toxicology Unit, Leicester, UK
    Epigenetics 7:432-9. 2012
    ..microRNA-mediated perturbation of such pathways may explain how harmful phenotypes can be induced in the progeny of smokers...
  76. ncbi Timing of establishment of paternal methylation imprints in the mouse
    Jing Yu Li
    Laboratory for Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
    Genomics 84:952-60. 2004
    ..5 in male germ cells. This study also indicates important differences between different imprinted DMR in timing and extent of methylation in the germ cells...
  77. ncbi Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function
    Nicole O Palmer
    School of Paediatrics and Reproductive Health, Discipline of Obstetrics and Gynaecology, University of Adelaide, South Australia, Australia
    Am J Physiol Endocrinol Metab 302:E768-80. 2012
    ....
  78. pmc Genome-wide analysis identifies changes in histone retention and epigenetic modifications at developmental and imprinted gene loci in the sperm of infertile men
    Saher Sue Hammoud
    Department of Oncological Sciences, Howard Hughes Medical Institute, Salt Lake City, UT, USA
    Hum Reprod 26:2558-69. 2011
    ..We hypothesize that changes in chromatin packaging may be associated with poor reproductive outcome...
  79. pmc The "soluble" adenylyl cyclase in sperm mediates multiple signaling events required for fertilization
    Kenneth C Hess
    Department of Pharmacology, Joan and Sanford Weill Medical College, Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA
    Dev Cell 9:249-59. 2005
    ..This inhibitor revealed that capacitation is defined by separable events: induction of protein tyrosine phosphorylation and motility are sAC dependent while acrosomal exocytosis is not dependent on sAC...
  80. ncbi Upregulation of clusterin in prostate and DNA damage in spermatozoa from bisphenol A-treated rats and formation of DNA adducts in cultured human prostatic cells
    Silvio De Flora
    Department of Health Sciences, University of Genoa, Genoa, Italy
    Toxicol Sci 122:45-51. 2011
    ..expression in atrophic prostate epithelial cells and induced lipid peroxidation and DNA fragmentation in spermatozoa. Significant levels of DNA adducts were formed in prostate cell lines treated either with high-dose BPA for 24 ..
  81. pmc Novel insights into DNA methylation features in spermatozoa: stability and peculiarities
    Csilla Krausz
    Department of Clinical Physiopathology, Andrology Unit, University of Florence, Florence, Italy
    PLoS ONE 7:e44479. 2012
    ....
  82. pmc Mechanism of human papillomavirus binding to human spermatozoa and fertilizing ability of infected spermatozoa
    Carlo Foresta
    Section of Clinical Pathology and Centre for Male Gamete Cryopreservation, Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy
    PLoS ONE 6:e15036. 2011
    ..These data show that sperm might function as vectors for HPV transfer into the oocytes, and open new perspectives on the role of HPV infection in males and are particularly intriguing in relation to assisted reproduction techniques...
  83. pmc Heads or tails? Structural events and molecular mechanisms that promote mammalian sperm acrosomal exocytosis and motility
    Mariano G Buffone
    Center for Research on Reproduction and Women s Health, Department of Obstetrics and Gynecology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Mol Reprod Dev 79:4-18. 2012
    ..Comprehending these processes will have a considerable impact on the management of fertility problems, the development of contraceptive methods, and, potentially, elucidation of analogous processes in other cell systems...
  84. ncbi Male obesity and alteration in sperm parameters
    Ahmad O Hammoud
    Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Utah, School of Medicine, Salt Lake City, Utah 84132, USA
    Fertil Steril 90:2222-5. 2008
    ..To study the effect of male obesity on sperm parameters and erectile dysfunction...
  85. ncbi Glioma pathogenesis-related 1-like 1 is testis enriched, dynamically modified, and redistributed during male germ cell maturation and has a potential role in sperm-oocyte binding
    Gerard M Gibbs
    Department of Anatomy and Developmental Biology, School of Biomedical Sciences, Monash University, Victoria 3800, Australia
    Endocrinology 151:2331-42. 2010
    ..Collectively these data further strengthen the role of CAP domain-containing proteins in cellular adhesion and propose a mechanism whereby CAP proteins show overlapping functional significance during fertilization...
  86. pmc Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility
    Kiyoshi Miki
    Laboratories for Reproductive Biology, and Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 101:16501-6. 2004
    ....
  87. ncbi The sperm nucleus: chromatin, RNA, and the nuclear matrix
    Graham D Johnson
    The Center for Molecular Medicine and Genetics, C S Mott Center, Wayne State University of Medicine, 275 East Hancock, Detroit, MI 48201, USA
    Reproduction 141:21-36. 2011
    ..Despite these changes, both human and mouse spermatozoa maintain low levels of nucleosomes that appear non-randomly distributed throughout the genome...
  88. ncbi RNA induction and inheritance of epigenetic cardiac hypertrophy in the mouse
    Kay D Wagner
    Inserm U636, F 06108 Nice, France
    Dev Cell 14:962-9. 2008
    ..These results highlight the diversity of RNA-mediated epigenetic effects and may provide a paradigm for clinical cases of familial diseases whose inheritance is not fully explained in Mendelian terms...
  89. ncbi Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouse
    Elke Heytens
    Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
    Reprod Biomed Online 21:742-9. 2010
    ..The presence of PLCzeta in the spermatozoa and testis of the wobbler mouse was evaluated by PLCzeta immunostaining and quantitative reverse-transcription ..
  90. ncbi Sperm DNA: organization, protection and vulnerability: from basic science to clinical applications--a position report
    Christopher L R Barratt
    Reproductive and Developmental Biology, Maternal and Child Health Science Laboratories, Centre for Oncology and Molecular Medicine, Ninewells Hospital, University of Dundee, Dundee DD1 9SY, UK
    Hum Reprod 25:824-38. 2010
    ..It highlights the biological, assay and clinical uncertainties that require further research and ends with a series of 5 key recommendations...
  91. ncbi Androgen receptor expression in the caput epididymal epithelium is essential for development of the initial segment and epididymal spermatozoa transit
    Laura O'Hara
    MRC Human Reproductive Sciences Unit, The Queen s Medical Research Institute, Edinburgh EH16 4TJ, UK
    Endocrinology 152:718-29. 2011
    ..epithelium androgen receptor knock-out mice accumulate cell debris, proteinaceous material, and, at later ages, spermatozoa in their efferent ducts, which prevents normal passage of spermatozoa from the testis into the cauda epididymis ..
  92. pmc Acp36DE is required for uterine conformational changes in mated Drosophila females
    Frank W Avila
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 106:15796-800. 2009
    ..Rather, consistent with their role in later steps of sperm storage, we show here that their presence can affect the outcome of sperm competition situations...
  93. ncbi High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies
    Mariano G Buffone
    University of Pennsylvania, Center for Research on Reproduction and Women s Health, Philadelphia, Pennsylvania, USA
    J Androl 30:552-8. 2009
    ..b>Spermatozoa from semen samples obtained from 15 consenting healthy donors were separated in 2 subpopulations, L45 (first ..
  94. pmc Specific epigenetic alterations of IGF2-H19 locus in spermatozoa from infertile men
    Celine Chalas Boissonnas
    Biology of Reproduction CECOS, Cochin Saint Vincent de Paul Hospital, AP HP, Department of Genetics and Development, Cochin Institute, University Paris Descartes, Paris, France
    Eur J Hum Genet 18:73-80. 2010
    ..Using this new classification of patients, the observed intrinsic imprinting defects of spermatozoa appear not to impair significantly the outcome of assisted reproductive technologies.
  95. ncbi Acquisition of the H19 methylation imprint occurs differentially on the parental alleles during spermatogenesis
    T L Davis
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Genomics 58:18-28. 1999
    ..Sequences proximal to the H19 promoter, which are methylated in spermatozoa and on the paternal allele in somatic cells, are differentially methylated in diploid, mitotic spermatogonia...
  96. pmc Non-random positioning of chromosomes in human sperm nuclei
    Irina A Zalenskaya
    The Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, VA 23507 1627, USA
    Chromosome Res 12:163-73. 2004
    In human spermatozoa, the arrangement of chromosomes is non-random. Characteristic features are association of centromeres in the interior chromocenter and peripheral location of telomeres...
  97. ncbi Behavioral response of human spermatozoa to a concentration jump of chemoattractants or intracellular cyclic nucleotides
    Anna Gakamsky
    Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel
    Hum Reprod 24:1152-63. 2009
    ..A major question in mammalian sperm chemotaxis is whether the cells sense a chemoattractant gradient by comparing the chemoattractant concentration between time points or between spatial points...
  98. ncbi Antioxidative potential of melatonin against mercury induced intoxication in spermatozoa in vitro
    Mandava V Rao
    Reproductive Physiology and Toxicology Unit, Department of Zoology and Bio Medical Technology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380 009, India
    Toxicol In Vitro 22:935-42. 2008
    ..The results suggest that graded doses of mercury elicit depletion of antioxidant defense system in sperm without altering the viability and melatonin treatment was found to significantly inhibit oxidative damage caused by mercury...
  99. ncbi Characterization of Sptrx, a novel member of the thioredoxin family specifically expressed in human spermatozoa
    A Miranda-Vizuete
    Department of Biosciences at Novum, Center for Biotechnology, Karolinska Institute, S 141 57 Huddinge, Sweden
    J Biol Chem 276:31567-74. 2001
    ..humans, named Sptrx (sperm-specific trx), the first with a tissue-specific distribution, located exclusively in spermatozoa. Sptrx open reading frame encodes for a protein of 486 amino acids composed of two clear domains: an N-terminal ..
  100. ncbi Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men
    A Zini
    Division of Urology, Department of Surgery, University of Toronto, Ontario, Toronto, Canada
    Fertil Steril 75:674-7. 2001
    ..To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters...
  101. ncbi Genomic and functional evolution of the Drosophila melanogaster sperm proteome
    Steve Dorus
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Nat Genet 38:1440-5. 2006
    ..The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure...

Research Grants63

  1. Defining the Mammalian Intercellular Bridge Interactome
    Martin M Matzuk; Fiscal Year: 2010
    ..begin to differentiate and continue to interconnect male germ cells up through formation of mature spermatozoa. Knockout of TEX14 disrupts intercellular bridges in male and female mice and causes sterility in males but not ..
  2. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
    ..In our preliminary studies we have shown that when spermatozoa are treated with various chemicals (detergents, DTT, exogenous DNA) or are subjected to mechanical insults (..
  3. ALTERNATIVE MRNA PROCESSING DURING SPERMATOGENESIS
    PETER CHALLONER; Fiscal Year: 1992
    ..of the changes in gene expression that are required for the developmental pathway that generates mature spermatozoa. Thus, defects in the trans- acting factors involved in alternative pre-MRNA processing in spermatids may be ..
  4. Validation of Novel Infertility Biomarker
    PETER SUTOVSKY SUTOVSKY; Fiscal Year: 2012
    ..have already established that SPTRX3 protein is carried exclusively by defective, morphologically deviant human spermatozoa. Our preliminary data demonstrate that the increased semen content of SPTRX3-carrying spermatozoa coincides ..
  5. Role of nuclear receptors in origin and differentiation of spermatozoa
    ADAM MICHAEL REITZEL; Fiscal Year: 2011
    ..is a complex developmental process where undifferentiated spermatogonia are differentiated into mature spermatozoa that are then able to fertilize oocytes...
  6. Secretory Vesicle Function During Spermatogenesis & Fertilization in C. elegans
    STEVEN W L apos HERNAULT; Fiscal Year: 2010
    ..The processes we study, in addition to functioning in sperm, also play a role in proper kidney function and bone formation. ..
  7. Structural Targeting Testis Specific Kinases (TSSK) 1 &2 for Male Contraception
    John C Herr; Fiscal Year: 2013
    ..and mice to the centrosomes of spermatids during flagellogenesis and TSKS persists in the centrioles of mature spermatozoa. TSSK2 phosphorylates a second flagellar substrate, the sperm axonemal central apparatus protein SPAG16L...
  8. Intercellular networks and luminal acidification in the male reproductive tract
    Sylvie Breton; Fiscal Year: 2013
    b>Spermatozoa acquire their motility and fertilization capacity as they traverse the epididymis...
  9. microRNA regulation of spermatogonial stem cell self-renewal and differentiation
    RALPH LAWRENCE BRINSTER; Fiscal Year: 2013
    ..The multistage differentiative process of SSCs into spermatogonia and spermatozoa requires a multitude of extra- and intracellular signaling cues and we hypothesize that miRs also regulate the ..
  10. Lectin Resistant Tumor Cells And Functional Glycomics
    PAMELA M STANLEY; Fiscal Year: 2013
    ..Therefore, we hypothesize that complex N-glycans are required in spermatids for differentiation into spermatozoa. We will test both hypotheses directly, and also determine the mechanism by which GnT1IP-L acts to target and ..
  11. 3-Dimensional modeling of basal cell function in pseudostratified epithelia
    Sylvie Breton; Fiscal Year: 2013
    ..The epididymis, which connects the testis to the vas deferens, is involved in the maturation and storage of spermatozoa and, therefore, plays a crucial role in male fertility...
  12. TRANSCRIPTIONAL REGULATION DURING SPERMIOGENESIS
    Prabhakara P Reddi; Fiscal Year: 2010
    During spermiogenesis, spermatids acquire the acrosome and flagellum, condense their chromatin and become spermatozoa. Precise expression of spermatid differentiation markers is critical for proper formation of sperm...
  13. The Role of Bovine Sperm Acrosomal Membrane-Matrix Complex in Fertilization
    Subir K Nagdas; Fiscal Year: 2013
    ..The acrosomal matrix and the acrosomal membrane of mature spermatozoa are each segregated into domains of unique structure, composition and function...
  14. Role of epididymal dendritic cells in male reproductive function
    Nicolas Da Silva; Fiscal Year: 2013
    ..understudied aspects of male reproductive physiology is the ability of the excurrent duct to protect maturing spermatozoa against an autoimmune response, while initiating very efficient immune activation against pathogens...
  15. Drying, Storing and Reanimating Egg Germinal Vesicles to Preserve Fertility
    Pierre Comizzoli; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The ability to preserve spermatozoa and eggs for a long period of time is critical for retaining fertility, addressing infertility and lengthening the reproductive life span of individuals...
  16. Device for Hyperfast Scalable Vitrification of Germplasm in Large Volumes
    IGOR KATKOV; Fiscal Year: 2013
    ..VF) is gaining in popularity with successful protocols being developed for many types of GP, including spermatozoa and stem cells...
  17. Mechanisms of Kinase-dependent Regulation of the V-ATPase in Kidney
    NURIA M PASTOR-SOLER; Fiscal Year: 2013
    ..of epithelial proton-secreting epididymal clear cells, which contribute to the acidic environment where spermatozoa mature...
  18. Nongenomic Androgen Signaling in Sertolli Cells
    William H Walker; Fiscal Year: 2010
    ..of Sertoli cell-round spermatid attachments and the retention of round spermatids, 3) the release of mature spermatozoa. Although testosterone had been known to be required for these functions, the mechanisms by which testosterone ..
  19. Protein Phosphatase Action in Mammalian Spermatogenesis and Sperm Function
    Srinivasan Vijayaraghavan; Fiscal Year: 2012
    ..in testis, PP1?2 is by far predominant in male germ cells, and is the sole PP1 isoform present in mammalian spermatozoa. The PP1?2 isoform has a unique 22 amino acid C-terminal tail...
  20. CarSpers:sperm-specific ion channels;targets for male contraceptives
    David E Clapham; Fiscal Year: 2013
    ..We have identified ion channels that are in spermatozoa, but not other tissues...
  21. A Testis Specific Regulatory Subunit of Protein Phosphatase-1
    Ann O Sperry; Fiscal Year: 2010
    ..by applicant): The final stage of sperm development, spermiogenesis, is essential for formation of viable spermatozoa;however, the molecular mechanisms underlying this process are largely unknown...
  22. Exposure To Polycyclic Aromatic Hydrocarbons And Male Sperm Quality
    HUEIWANG ANNA COOK JENG; Fiscal Year: 2010
    ..We propose a longitudinal study to assess the effect of PAHs on male spermatozoa of human subjects...
  23. Understanding genetics of spermatogenic failure by resequencing the sex chromosome
    RENEE GEORGE; Fiscal Year: 2013
    ..by applicant): In spermatogenesis, undifferentiated male germ cells undergo meiosis to produce haploid spermatozoa. This process is extremely complex and a disruption at any stage can result in spermatogenic failure-a leading ..
  24. Transposon Based Mammalian Transgenesis and Transfection
    Stefan Moisyadi; Fiscal Year: 2012
    ..by far the most efficient method when introducing the transgene in a transposon along with non freeze-thawed spermatozoa into unfertilized oocytes (TN:ICSI)...
  25. Epigenetic Defects in Human Spermatozoa: Biomarker of Infertility and Sperm Toxic
    REBECCA Z contact SOKOL; Fiscal Year: 2010
    ..factor infertility and the ability to diagnose male infertility by investigating the epigenetic state of human spermatozoa and defining its physiologic role in male infertility...
  26. Water and solute transport in the epididymis
    Sylvie Breton; Fiscal Year: 2010
    ..neutral solute transport and is responsible for the establishment of an optimum luminal osmolarity as well as spermatozoa concentration;and 2) The basolateral membrane of the epididymal epithelium contains an as yet unidentified ..
  27. Regulation of Postnatal Epididymal Cell Proliferation
    Barry T Hinton; Fiscal Year: 2013
    ..applicant): The epididymis, and in particular, the initial segment, play an important role in the maturation of spermatozoa and without a fully developed initial segment, male infertility will result...
  28. Molecular Mechanisms that Control Ca2+ Signaling in Human Spermatozoa
    Yuriy Kirichok; Fiscal Year: 2013
    ..overcome this barrier and developed a method to apply the whole-cell patch clamp technique to mouse and human spermatozoa. Surprisingly, our patch-clamp experiments revealed significant differences between ion channels in mouse and ..
  29. Polycystins and Male Fertility
    Keith A Sutton; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Successful fertilization requires the arrival of the spermatozoa at the oocyte in a fertile state;the process of capacitation thus represents one of the key aspects of male fertility...
  30. Exploring the Role of Semen Amyloids in Promoting HIV Infection and Fertilization
    Nadia R Roan; Fiscal Year: 2013
    ..My long-term goal is to advance our understanding of the molecular events surrounding HIV transmission in the genital mucosa, and to translate this knowledge into the development of new classes of HIV preventatives. ..
  31. Control of Cell Differentiation: Inhibition of Sperm Activation in C. elegans
    Craig W LaMunyon; Fiscal Year: 2012
    ..In addition, this research has the potential to provide insight into the workings disease gene homologs, such as spe-4 (Presenilin-1;Alzheimer's disease) and fer-1 (Dysferlin family genes;muscular dystrophy). ..
  32. Establishment of a transgenic monkey of Huntingtons's diesease
    Anthony Wing Sang Chan; Fiscal Year: 2013
    ..molecular and cellular characteristics in transgenic HD monkeys, and (3) Cryopreservation of HD monkey's spermatozoa and embryos...
  33. Na,K-ATPase alpha4 isoform in male germ cell physiology
    V GUSTAVO BLANCO; Fiscal Year: 2010
    ..Importantly, 4 is necessary for normal motility of spermatozoa. Supporting its biological importance, we have shown that 4 undergoes significant developmental changes, and ..
  34. MMRRC INFORMATICS, COORDINATION, AND SERVICE CENTER AT UC DAVIS
    ERIC KENNETH ENGELHARD; Fiscal Year: 2011
    ..support, and software tools and web interfaces to support the curation of the large repository frozen embryos, spermatozoa, embryonic stem (ES) cell lines, and living mice...
  35. A Novel, Inexpensive Cryopreservation System for Stable -80 degree C Storage of A
    Erik J Woods; Fiscal Year: 2011
    ..cooling device that allow the long-term storage and safe shipment of animal model germplasm (embryos and spermatozoa) and embryonic stem cells (ESCs) at -80oC...
  36. Regulation of C. elegans Sperm Differentiation
    GILLIAN STANFIELD; Fiscal Year: 2012
    ..We are using C. elegans to study one such signal that is mediated by proteases, an important family of enzymes that is present in all animals and in humans. ..
  37. MMRRC INFORMATICS, COORDINATION, AND SERVICE CENTER AT UC DAVIS
    ERIC KENNETH ENGELHARD; Fiscal Year: 2013
    ..support, and software tools and web interfaces to support the curation of the large repository frozen embryos, spermatozoa, embryonic stem (ES) cell lines, and living mice...
  38. HORMONAL REGULATION OF SERTOLI CELL MATURATION
    Michael D Griswold; Fiscal Year: 2013
    ..provided by applicant): A fundamental feature of mammalian spermatogenesis is the continual production of spermatozoa within the testis throughout the reproductive lifetime of the animal...
  39. ACROSOMAL ENZYMES AND THEIR ROLE IN FERTILIZATION
    PRAKASH SRIVASTAVA; Fiscal Year: 1993
    ..The fusion of spermatozoa with vitelline membrane probably involves phospholipases of the persistent PM and OAM on the equatorial segment ..
  40. REGULATION AND FUNCTION OF SPARC IN MALE REPRODUCTION
    Robert Vernon; Fiscal Year: 1991
    ..the nongerminal Sertoli, Leydig, and peritubular cells that create a unique environment for the maturation of spermatozoa. A calcium-binding protein, SPARC (Secreted Protein, Acidic and Rich in Cysteine) has been identified in the ..
  41. REGULATION AND FUNCTION OF HEAT SHOCK FACTORS IN TESTIS
    KEVIN SARGE; Fiscal Year: 1999
    ..mitosis, meiosis, and differentiation of distinct spermatogenic cell types, are transformed into mature spermatozoa capable of fertilizing an ovum...
  42. TISSUE SPECIFIC EXPRESSION AND FUNCTION OF ME-RABP
    Marie Claire Orgebin Crist; Fiscal Year: 2002
    In the epididymis spermatozoa mature, gain the ability to fertilize in vivo and are stored prior to ejaculation. Vitamin A is important in epididymal function since it is required to maintain epithelial structures...
  43. LYSOSOMAL PROTEASES OF SPERMATOZOA
    JOHN MC DONALD; Fiscal Year: 1993
    ..of new lysosomal-type protease (endopeptidases and exopeptidases) recently detected in guinea pig epididymal spermatozoa. One of the endopeptidases resembles lysosomal cathepsin L, but is totally masked in epididymal sperm...
  44. YOUNG FROM STORED AND IMPAIRED MOUSE SPERM
    John Biggers; Fiscal Year: 2001
    ..algorithm using assisted reproductive techniques to reconstitute valuable genetic strains of mice from stored spermatozoa. The ability to store spermatozoa is urgently needed because of the exponentially increasing numbers of mutant ..
  45. Role of Cyclic Nucleotides in Sperm Function
    Marco Conti; Fiscal Year: 2009
    ..a membrane-bound AC3 and soluble adenylyl cyclases (sAC), contribute to cAMP production in spermatids and in spermatozoa. The properties of sAC have been extensively investigated demonstrating that this cyclase is integrated in ..
  46. FUNDAMENTAL CRYOBIOLOGY OF HUMAN SPERMATOZOA
    John Critser; Fiscal Year: 1992
    ..Information regarding why spermatozoa from some men freeze will while others freeze very poorly will be addressed and development of a "standard ..
  47. HUMAN SPERM ENZYMES, INHIBITORS AND FERTILIZATION
    Lourens Zaneveld; Fiscal Year: 1991
    ..at neutral or basic pH is entirely due to the dialyzable acrosin inhibitor(s) known to be associated with spermatozoa or also to other factors...
  48. REGULATION OF SPERM FUNCTION BY PROTEIN PRENYLATION
    Gary Olson; Fiscal Year: 1999
    ..Spermatids and spermatozoa possess both farnesyltransferase (Ftase), which functions in post-translational protein lipidation, and the ..
  49. Role of GPI-Anchored CD52 Glycoprotein in Sperm Function
    Alan Diekman; Fiscal Year: 2003
    ..In spermatozoa, insights into the functional role of CD52 have been obtained using S19, a monoclonal antibody (mAb) specific ..
  50. MALE REPRODUCTIVE TOXICITY AND ESTROGENICITY OF PAHS
    Samir Raychoudhury; Fiscal Year: 1999
    ..hypothesis is that PAHs cause testicular cytoarchitectural disruption perturbing final development of mature spermatozoa. Particular subclasses of male germ cells will be examined to assess the damage from the histological ..
  51. GENETIC BASIS OF SPERMATOLOGICAL STEM CELL RENEWAL
    COLIN BISHOP; Fiscal Year: 2001
    ..of cell division and differentiation by which spermatogonial stem cells give rise to large numbers of mature spermatozoa. It is an essential determinant of fertility in all mammals, playing a fundamental role in the perpetuation of ..
  52. PROSTAGLANDINS AND EPIDIDYMAL SPERM MATURATION
    LEGRANDE ELLIS; Fiscal Year: 1980
    ..in the epididymal fluids, spermatozoal adenyl cyclase activity, spermatozoal cAMP levels and maturation of the spermatozoa as these cells pass through the epididymis and into the vas deferens...
  53. STORAGE OF MOUSE STRAINS USING SPERM CRYOPRESERVATION
    John Critser; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): Although mouse spermatozoa were "successfully" frozen more than a decade ago, the fundamental cryobiological factors that affect the ability of spermatozoa to survive with full function ..
  54. IMMUNOLOGICAL STUDIES OF THE MALE REPRODUCTIVE SYSTEM
    Stephen Byers; Fiscal Year: 1990
    Developing germ cells in the testis and maturing spermatozoa in the epididymis are largely sheltered from the systemic immune system...
  55. FUNCTIONAL ASPECTS OF SPERM CHROMATIN STRUCTURE
    William Ward; Fiscal Year: 2000
    ..This has significant implications for research in many areas such as the manipulation of spermatozoa for in vitro fertilization (IVF), the development of male contraceptives, sexual transmission of diseases, the ..
  56. Optimizing Testis Transplantation to Study RET Signaling
    Cathy Naughton; Fiscal Year: 2005
    ..This technology has been used to mature testicular germ cells into spermatozoa, which have the ability to cause fertilization by intracytoplasmic sperm injection into species-specific ..
  57. CRYOBANKING OF MOUSE SPERMATOZOA
    Jerome Sherman; Fiscal Year: 1980
    Recent unpublished observations have established the predicted capacity of frozen-thawed mouse spermatozoa to fertilize and to induce the development of normal progeny...
  58. THE ROLE OF THE EPIDIDYMIS IN SPERMATOZOA MATURATION
    ROY HAMMERSTEDT; Fiscal Year: 1990
    ....
  59. SPERMATOGONIAL STEM CELL CULTURE AND TRANSPLANTATION
    RALPH BRINSTER; Fiscal Year: 2002
    ..transfer, the donor cell population establishes normal spermatogenesis in the recipient testes, and mature spermatozoa are produced and found in the epididymis...
  60. FUNCTIONS OF PROTEIN DOMAINS IN GAMETE INTERACTIONS
    Daniel Hardy; Fiscal Year: 2002
    ..and biogenesis that are relevant to the hypothesis that this protein mediates species-specific adhesion of spermatozoa to the ZP...
  61. BIOCHEMISTRY AND PHYSIOLOGY OF RODENT ABP
    BENJAMIN DANZO; Fiscal Year: 1990
    ..is a Serotoli cell product though to be involved in spermatogenesis, the acquisition of fertilizing ability by spermatozoa, and/or the regulation of epididymal function...
  62. ROLE OF GLUTATHIONE IN MALE REPRODUCTIVE TOXICITY
    Jay Gandy; Fiscal Year: 1993
    ..chemical-induced reduction of glutathione in the male reproductive tract with increased susceptibility of spermatozoa to chemical-induced mutations...
  63. DNA SYNTHESIZING COMPLEXES IN SEMEN
    Steven Witkin; Fiscal Year: 1980
    DNA-synthesizing complexes are components of human, bovine and rooster spermatozoa. Their presence in all human ejaculates examined plus the existence of autoantibodies to the complex-associated DNA polymerase in some individual members ..