gastrula

Summary

Summary: The embryo in the early stage following the blastula, characterized by morphogenetic cell movements, cell differentiation, and the formation of the three germ layers.

Top Publications

  1. ncbi Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation
    C P Heisenberg
    Department of Anatomy and Developmental Biology, University College, London, UK
    Nature 405:76-81. 2000
  2. ncbi Expression of zebrafish goosecoid and no tail gene products in wild-type and mutant no tail embryos
    S Schulte-Merker
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Tubingen, Germany
    Development 120:843-52. 1994
  3. ncbi Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo
    H Sasaki
    Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232
    Development 118:47-59. 1993
  4. ncbi Molecular genetics of axis formation in zebrafish
    Alexander F Schier
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016 6497, USA
    Annu Rev Genet 39:561-613. 2005
  5. ncbi Wnt-3a regulates somite and tailbud formation in the mouse embryo
    S Takada
    Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110
    Genes Dev 8:174-89. 1994
  6. ncbi Initiation of neural induction by FGF signalling before gastrulation
    A Streit
    Department of Genetics and Development, Columbia University, New York, New York 10032, USA
    Nature 406:74-8. 2000
  7. ncbi Expression pattern of the mouse T gene and its role in mesoderm formation
    D G Wilkinson
    Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research, London, UK
    Nature 343:657-9. 1990
  8. pmc Dorsal-ventral patterning and neural induction in Xenopus embryos
    Edward M De Robertis
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Annu Rev Cell Dev Biol 20:285-308. 2004
  9. ncbi A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse
    F L Conlon
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
    Development 120:1919-28. 1994
  10. ncbi The transcription factor HNF3beta is required in visceral endoderm for normal primitive streak morphogenesis
    D Dufort
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5
    Development 125:3015-25. 1998

Research Grants

  1. Morphogenesis of mammalian gut endoderm
    Anna Katerina Hadjantonakis; Fiscal Year: 2012
  2. Identification of Mechanically Sensitive Proteins in Early Development
    Douglas W DeSimone; Fiscal Year: 2013
  3. Identification of Binding Proteins of the Xenopus Morphogenetic Factor Tumorhead
    Edwin E Traverso; Fiscal Year: 2012
  4. Regulation of call adhesion in Xenopus
    KEN CHO; Fiscal Year: 2009
  5. GENETIC ANALYSIS OF CONVERGENT EXTENSION IN ZEBRAFISH
    Lilianna Solnica-Krezel; Fiscal Year: 2013
  6. DORSAL-VENTRAL PATTERN FORMATION IN THE ZEBRAFISH EMBRYO
    Mary C Mullins; Fiscal Year: 2013
  7. Molecular Interactions During Neural Crest Formation
    MARTIN IGNACIO GARCIA-CASTRO; Fiscal Year: 2013
  8. Regulation of Planar Cell Polarity in Migrating Cells
    JASON ROBERT JESSEN; Fiscal Year: 2013
  9. Mammalian Foregut and Liver Development
    Aaron M Zorn; Fiscal Year: 2012
  10. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2013

Detail Information

Publications293 found, 100 shown here

  1. ncbi Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation
    C P Heisenberg
    Department of Anatomy and Developmental Biology, University College, London, UK
    Nature 405:76-81. 2000
    ..Our results provide genetic and experimental evidence that Wnt activity in lateral tissues has a crucial role in driving the convergent extension movements underlying vertebrate gastrulation...
  2. ncbi Expression of zebrafish goosecoid and no tail gene products in wild-type and mutant no tail embryos
    S Schulte-Merker
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Tubingen, Germany
    Development 120:843-52. 1994
    goosecoid is an immediate early gene expressed at the dorsal blastoporal lip of the Xenopus gastrula. Microinjection experiments have suggested a direct role for goosecoid in organizing the dorsoventral axis of the frog embryo...
  3. ncbi Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo
    H Sasaki
    Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232
    Development 118:47-59. 1993
    ..A model is proposed linking FD gene expression with gastrulation events in the mouse...
  4. ncbi Molecular genetics of axis formation in zebrafish
    Alexander F Schier
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016 6497, USA
    Annu Rev Genet 39:561-613. 2005
    ..the expression of transcription factors and the location of signaling centers that pattern the blastula and gastrula. Bmp, Nodal, FGF, canonical Wnt, and retinoic acid signals generate positional information that leads to the ..
  5. ncbi Wnt-3a regulates somite and tailbud formation in the mouse embryo
    S Takada
    Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110
    Genes Dev 8:174-89. 1994
    ..We suggest that dysmorphology is secondary to the mesodermal and axial defects and that dorsal patterning of the CNS may be regulated by inductive signals arising from surface ectoderm...
  6. ncbi Initiation of neural induction by FGF signalling before gastrulation
    A Streit
    Department of Genetics and Development, Columbia University, New York, New York 10032, USA
    Nature 406:74-8. 2000
    ..This process is generally thought to occur around the mid-gastrula stage of embryogenesis...
  7. ncbi Expression pattern of the mouse T gene and its role in mesoderm formation
    D G Wilkinson
    Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research, London, UK
    Nature 343:657-9. 1990
    ..This expression pattern correlates with the tissues affected in the T-gene mutant, and indicates that the T gene has a direct role in the early events of mesoderm formation and in the morphogenesis of the notochord...
  8. pmc Dorsal-ventral patterning and neural induction in Xenopus embryos
    Edward M De Robertis
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Annu Rev Cell Dev Biol 20:285-308. 2004
    ..When dorsal mesoderm is formed at gastrula, a cocktail of growth factor antagonists is secreted by the Spemann organizer and further patterns the embryo...
  9. ncbi A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse
    F L Conlon
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
    Development 120:1919-28. 1994
    ..Data presented in this report demonstrate the involvement of a TGF beta-related molecule in axis formation in mammals...
  10. ncbi The transcription factor HNF3beta is required in visceral endoderm for normal primitive streak morphogenesis
    D Dufort
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5
    Development 125:3015-25. 1998
    ..We show that such mutant embryos lack foregut and midgut endoderm. In addition, left-right asymmetry is affected in the mutant embryos...
  11. ncbi Zebrafish organizer development and germ-layer formation require nodal-related signals
    B Feldman
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University Medical Center, New York 10016, USA
    Nature 395:181-5. 1998
    ..Misexpression of sqt RNA within the embryo or specifically in the YSL induces expanded or ectopic dorsal mesoderm. These results establish an essential role for nodal-related signals in organizer development and mesendoderm formation...
  12. pmc Proposal of a model of mammalian neural induction
    Ariel J Levine
    Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, New York, NY 10021, USA
    Dev Biol 308:247-56. 2007
    ..In this model, the gastrula organizer of the mouse embryo inhibits BMP signaling to allow neural tissue to form as a default fate-in the ..
  13. ncbi Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer
    T Bouwmeester
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, 90095 1737, USA
    Nature 382:595-601. 1996
    ..The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo...
  14. ncbi The organizer of the mouse gastrula is composed of a dynamic population of progenitor cells for the axial mesoderm
    S J Kinder
    Embryology Unit, Children s Medical Research Institute, Locked Bag 23, Wentworthville, NSW 2145, Australia
    Development 128:3623-34. 2001
    ..This cell population can therefore be regarded as the mid-gastrula organizer and, together with the early-gastrula organizer and the node, constitute the organizer of the mouse ..
  15. ncbi FGF8-like1 and FGF8-like2 encode putative ligands of the FGF receptor Htl and are required for mesoderm migration in the Drosophila gastrula
    Tanja Gryzik
    Institut fur Genetik, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
    Curr Biol 14:659-67. 2004
    Mesoderm migration in the Drosophila gastrula depends on the fibroblast growth factor (FGF) receptor Heartless (Htl)...
  16. ncbi Formation and function of Spemann's organizer
    R Harland
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Annu Rev Cell Dev Biol 13:611-67. 1997
    ..Final steps of organizer formation at the late blastula or early gastrula stage may involve locally secreted zygotic signals as well...
  17. ncbi Differential regulation of chordin expression domains in mutant zebrafish
    V E Miller-Bertoglio
    Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Dev Biol 192:537-50. 1997
    ..1989), while axial mesodermal expression was affected. This finding reveals an unexpected function for spadetail in midline mesoderm and in differential regulation of chordin expression during gastrulation...
  18. pmc A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo
    A Simeone
    International Institute of Genetics and Biophysics, CNR, Naples, Italy
    EMBO J 12:2735-47. 1993
    ..Its gene product contains a homeodomain of the bicoid class and is able to recognize and transactivate a bicoid target sequence...
  19. ncbi Gastrulation in the mouse: the role of the homeobox gene goosecoid
    M Blum
    Molecular Biology Institute, University of California, Los Angeles 90024 1737
    Cell 69:1097-106. 1992
    ..end of the developing primitive streak, and finally in the anteriormost mesoderm at the tip of the early mouse gastrula, a region that gives rise to the head process...
  20. ncbi Control of gastrula cell motility by the Goosecoid/Mix.1/ Siamois network: basic patterns and paradoxical effects
    Olivia Luu
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Dev Dyn 237:1307-20. 2008
    In the vegetal half of the Xenopus gastrula, cell populations differ with respect to migration on fibronectin substratum. We show that the paired-class homeodomain transcription factors Goosecoid (Gsc), Mix...
  21. ncbi Spatial restriction of bone morphogenetic protein signaling in mouse gastrula through the mVam2-dependent endocytic pathway
    Minako Aoyama
    Division of Biological Science, Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, Osaka 567 0047, Japan
    Dev Cell 22:1163-75. 2012
    ..We found that mVam2, which interacts with BMP type I receptor, is required for the spatiotemporal modulation of BMP signaling, via sequestration of the receptor complex in the late stages of the endocytic pathway...
  22. ncbi Distinct regulatory control of the Brachyury gene in axial and non-axial mesoderm suggests separation of mesoderm lineages early in mouse gastrulation
    D Clements
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Mech Dev 56:139-49. 1996
    ..Additionally, the data suggest the possibility that axial and non-axial mesoderm are distinct from the earliest stages of Brachyury expression...
  23. ncbi Wnt/Frizzled signaling requires dPRR, the Drosophila homolog of the prorenin receptor
    Tina Buechling
    Division of Signaling and Functional Genomics, German Cancer Research Center DKFZ, and Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, University of Heidelberg, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Curr Biol 20:1263-8. 2010
    ..Furthermore, dPRR binds to Fz and Fz2 receptors. In summary, our data suggest that dPRR has an evolutionarily conserved role at the receptor level for activation of canonical and noncanonical Wnt/Fz signaling pathways...
  24. ncbi Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation
    X Zhou
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Nature 361:543-7. 1993
    ..Our findings suggest that this gene, named nodal, encodes a signalling molecule essential for mesoderm formation and subsequent organization of axial structures in early mouse development...
  25. ncbi Regulatory blueprint for a chordate embryo
    Kaoru S Imai
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto, 606 8502, Japan
    Science 312:1183-7. 2006
    ..These diagrams constitute a blueprint for the Ciona embryo and provide a foundation for understanding the evolutionary origins of the chordate body plan...
  26. ncbi How we are shaped: the biomechanics of gastrulation
    Ray Keller
    Department of Biology, University of Virginia, P O Box 400328, Charlottesville, VA 22904 4328, USA
    Differentiation 71:171-205. 2003
    ..Our goal is to review what is known and identify problems for further research...
  27. ncbi Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation
    D Acampora
    International Institute of Genetics and Biophysics CNR, Naples, Italy
    Development 121:3279-90. 1995
    ....
  28. ncbi Expression of the novel murine homeobox gene Sax-1 in the developing nervous system
    F R Schubert
    Abteilung für Molekulare Zellbiologie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Mech Dev 51:99-114. 1995
    ..0 pc. Ventral shift and finally loss of the signal in the ventral spinal cord was observed in Danforth's short tail homozygotes...
  29. ncbi The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates and specifies anterior body regions
    M Pannese
    DIBIT, Istituto Scientifico H S Raffaele, Milano, Italy
    Development 121:707-20. 1995
    ..Microinjected transcripts of Xotx2 constructs containing a homeodomain where this lysine is substituted by a glutamine or a glutamic acid residue fail to cause these effects...
  30. ncbi Cell movement patterns during gastrulation in the chick are controlled by positive and negative chemotaxis mediated by FGF4 and FGF8
    Xuesong Yang
    Division of Cell and Developmental Biology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    Dev Cell 3:425-37. 2002
    ..The observed movement patterns of anterior streak cells can be explained by an FGF8-mediated chemorepulsion of cells away from the streak followed by chemoattraction toward an FGF4 signal produced by the forming notochord...
  31. ncbi Bmp activity gradient regulates convergent extension during zebrafish gastrulation
    Dina C Myers
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, Tennessee 37235 1634, USA
    Dev Biol 243:81-98. 2002
    ..cell populations in vivo, we reveal the presence of three domains of convergent extension movements in zebrafish gastrula. Ventrally, convergence and extension movements are absent...
  32. pmc PKC delta is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements
    Noriyuki Kinoshita
    Department of Developmental Biology, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444 8585, Japan
    Genes Dev 17:1663-76. 2003
    ..Thus, PKC delta plays an essential role in the Wnt/JNK pathway by regulating the localization and activity of Dishevelled...
  33. ncbi The ventral and posterior expression of the zebrafish homeobox gene eve1 is perturbed in dorsalized and mutant embryos
    J S Joly
    Unite de Genetique des Mammiferes, Institut Pasteur, Paris, France
    Development 119:1261-75. 1993
    ..eve1 RNAs are expressed initially in late blastulae with a peak during the gastrula stage, at which time expression is confined to ventral and lateral cells of the marginal zone of the zebrafish ..
  34. ncbi Live imaging and genetic analysis of mouse notochord formation reveals regional morphogenetic mechanisms
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Dev Cell 13:884-96. 2007
    ..Therefore, we propose three distinct regions within the mouse notochord, each with unique morphogenetic origins...
  35. ncbi A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula
    M Fürthauer
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, ULP, Illkirch, France
    Development 124:4253-64. 1997
    ..fgf-8, not secreted by the axial mesoderm but expressed in a dorsoventral gradient at the margin of the zebrafish gastrula, also contributes to the establishment of the dorsoventral axis of the embryo...
  36. ncbi Goosecoid promotes head organizer activity by direct repression of Xwnt8 in Spemann's organizer
    J Yao
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Development 128:2975-87. 2001
    ..Therefore, Gsc promotes head organizer activity by direct repression of Xwnt8 in Spemann's organizer and this activity is essential for anterior development...
  37. pmc Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane
    Sabine Witzel
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 175:791-802. 2006
    ..We propose that Wnt11, by interacting with Frizzled 7 and Flamingo, modulates local cell contact persistence to coordinate cell movements during gastrulation...
  38. pmc Noncanonical Wnt signaling regulates midline convergence of organ primordia during zebrafish development
    Takaaki Matsui
    Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Genes Dev 19:164-75. 2005
    ..Overall, our results uncover a late, previously unexpected role of noncanonical Wnt signaling in the control of midline assembly of organ precursors during vertebrate embryo development...
  39. ncbi Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse
    G Winnier
    Howard Hughes Medical Institute, Vanderbilt University Medical School, Nashville, Tennessee 37232 2175, USA
    Genes Dev 9:2105-16. 1995
    ..Moreover, in the presumed absence of zygotic ligand, it appears that homozygous mutants can be rescued partially by related proteins or by maternal BMP-4...
  40. pmc Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains
    Tetsuhiro Kudoh
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 131:3581-92. 2004
    ..and amphibia have shown that graded Bmp signalling activity regulates dorsal-to-ventral (DV) patterning of the gastrula embryo...
  41. ncbi Nodal antagonists in the anterior visceral endoderm prevent the formation of multiple primitive streaks
    Aitana Perea-Gomez
    IGBMC CNRS INSERM, Universite Louis Pasteur, 67404 Illkirch Cedex, CU de, Strasbourg, France
    Dev Cell 3:745-56. 2002
    ..Both antagonists are also required for proper patterning of the primitive streak...
  42. ncbi The zebrafish glypican knypek controls cell polarity during gastrulation movements of convergent extension
    J Topczewski
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA
    Dev Cell 1:251-64. 2001
    ....
  43. ncbi Dissecting Wnt/beta-catenin signaling during gastrulation using RNA interference in mouse embryos
    Heiko Lickert
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M5G 1X5, Canada
    Development 132:2599-609. 2005
    ..This functional genomic approach allows the rapid identification of functionally important components of embryonic development from large datasets of putative targets...
  44. ncbi Posteriorization by FGF, Wnt, and retinoic acid is required for neural crest induction
    Sandra Villanueva
    Millennium Nucleus in Developmental Biology, Facultad de Sciencias, Universidad de Chile, Santiago, Chile
    Dev Biol 241:289-301. 2002
    ..We discuss a unifying model where lateralizing and posteriorizing signals are presented as two stages of the same inductive process required for neural crest induction...
  45. ncbi Conserved and divergent roles for members of the Snail family of transcription factors in the chick and mouse embryo
    M Sefton
    Instituto Cajal, CSIC, Avenida Doctor Arce, 28002 Madrid, Spain
    Development 125:3111-21. 1998
    ..Furthermore, several early sites of Slu and Sna expression have been swapped in the avian lineage. Our analysis of the Snail family may also shed new light on the origin of the neural crest...
  46. pmc Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives
    Owen J Tamplin
    Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    BMC Genomics 9:511. 2008
    The Spemann/Mangold organizer is a transient tissue critical for patterning the gastrula stage vertebrate embryo and formation of the three germ layers...
  47. ncbi Zebrafish pou5f1/pou2, homolog of mammalian Oct4, functions in the endoderm specification cascade
    Karen Lunde
    Developmental Biology, Institute Biology 1, University of Freiburg, Hauptstrasse 1, D 79104, Freiburg, Germany
    Curr Biol 14:48-55. 2004
    ..We propose that pou5f1 plays an activating role in zebrafish endodermal development, where it maintains sox32 expression during gastrulation and acts with sox32 to induce sox17 expression in endodermal precursor cells...
  48. ncbi Conserved requirement of Lim1 function for cell movements during gastrulation
    Neil A Hukriede
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 4:83-94. 2003
    ..We conclude that a primary function of Lim1 in the early embryo is to enable proper cell movements during gastrulation...
  49. ncbi Gastrulation in Drosophila: the formation of the ventral furrow and posterior midgut invaginations
    D Sweeton
    Department of Molecular Biology, Princeton University, New Jersey 08544
    Development 112:775-89. 1991
    ..Mutations in the concertina or folded gastrulation genes appear to block the transition to the second phase in both the ventral furrow and the posterior midgut invaginations...
  50. ncbi The Wnt-activated Xiro1 gene encodes a repressor that is essential for neural development and downregulates Bmp4
    J Gomez-Skarmeta
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, Cantoblanco, Spain
    Development 128:551-60. 2001
    ..Consistently, Xiro1 and Bmp4 have complementary patterns of expression during gastrulation. The expression of Xiro1 requires Wnt signaling. Thus, Xiro1 is probably a mediator of the known downregulation of Bmp4 by Wnt signaling...
  51. ncbi Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula
    J A Belo
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of California, Los Angeles 90095 1662, USA
    Mech Dev 68:45-57. 1997
    ..A model of how head and trunk development might be regulated is discussed. Given its neuralizing activity, the secreted protein Cer-l is a candidate for mediating inductive activities of anterior visceral endoderm...
  52. ncbi Zinc transporter LIVI controls epithelial-mesenchymal transition in zebrafish gastrula organizer
    Susumu Yamashita
    Laboratory of Developmental Immunology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 429:298-302. 2004
    ..The requirement for STAT3 is cell-autonomous for the anterior migration of gastrula organizer cells, and non-cell-autonomous for the convergence of neighbouring cells...
  53. ncbi Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene
    G von Dassow
    Department of Biochemistry, University of Washington, Seattle 98195
    Genes Dev 7:355-66. 1993
    ..We describe the cloning and expression of Xnot, a novel homeo box-containing gene expressed primarily in the gastrula organizing region...
  54. ncbi Regulation of neural induction by the Chd and Bmp-4 antagonistic patterning signals in Xenopus
    Y Sasai
    Howard Hughes Medical Institute, University of California, Los Angeles 90095 1737, USA
    Nature 376:333-6. 1995
    ..We suggest that conserved molecular mechanisms involving chd/sog and bmp-4/dpp gene products pattern the ectoderm in Xenopus and in Drosophila...
  55. ncbi Acquisition of Hox codes during gastrulation and axial elongation in the mouse embryo
    Sylvie Forlani
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Development 130:3807-19. 2003
    ....
  56. pmc Regulation of the Rac GTPase pathway by the multifunctional Rho GEF Pebble is essential for mesoderm migration in the Drosophila gastrula
    Andreas van Impel
    Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee, UK
    Development 136:813-22. 2009
    ..Furthermore, gain-of-function and rescue experiments suggest an important regulatory role of the C-terminal tail of Pbl for the selective activation of Rho1-versus Rac-dependent pathways...
  57. ncbi Tcf- and Vent-binding sites regulate neural-specific geminin expression in the gastrula embryo
    Jennifer J Taylor
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Dev Biol 289:494-506. 2006
    ..However, it is unknown for any vertebrate gene how the onset of neural-specific expression in early gastrula embryos is transcriptionally regulated...
  58. ncbi Genetic dissection of nodal function in patterning the mouse embryo
    L A Lowe
    Experimental Immunology Branch, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Development 128:1831-43. 2001
    ..We find that nodal signaling is required for correct positioning of the anteroposterior axis, normal anterior and midline patterning, and the left-right asymmetric development of the heart, vasculature, lungs and stomach...
  59. ncbi Fate mapping the mouse embryo
    K A Lawson
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht
    Int J Dev Biol 43:773-5. 1999
    ..A revised fate map of the epiblast at 6.5 days gestation is provided, and the development of 3-dimensional, quantitative image analysis techniques outlined...
  60. ncbi Definitive endoderm of the mouse embryo: formation, cell fates, and morphogenetic function
    Samara L Lewis
    Embryology Unit, Children s Medical Research Institute, University of Sydney, Sydney, NSW, Australia
    Dev Dyn 235:2315-29. 2006
    ....
  61. ncbi The Bmp gradient of the zebrafish gastrula guides migrating lateral cells by regulating cell-cell adhesion
    Sophia von der Hardt
    Max Planck Institute of Immunobiology, D 79108 Freiburg, Germany
    Curr Biol 17:475-87. 2007
    ....
  62. ncbi FGF signaling regulates mesoderm cell fate specification and morphogenetic movement at the primitive streak
    B Ciruna
    Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada
    Dev Cell 1:37-49. 2001
    ..We propose that modulation of cytoplasmic beta-catenin levels, associated with FGF-induced downregulation of E-cadherin, provides a molecular link between FGF and Wnt signaling pathways at the streak...
  63. ncbi Smad2 role in mesoderm formation, left-right patterning and craniofacial development
    M Nomura
    Cardiovascular Research Center, Massachusetts General Hospital East, Department of Medicine, Harvard Medical School, Charlestown 02129, USA
    Nature 393:786-90. 1998
    ..Our results show that Smad2 function is essential for early development and for several patterning processes in mice...
  64. ncbi Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening
    Jianwu Bai
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Protoc 4:1502-12. 2009
    ..The entire protocol takes approximately 14 d, whereas the preparation of primary cells from Drosophila embryos only requires 2-4 h...
  65. ncbi The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo
    P H Crossley
    Department of Anatomy and Program in Developmental Biology, School of Medicine, University of California at San Francisco 94143 0452, USA
    Development 121:439-51. 1995
    ....
  66. ncbi The prechordal region lacks neural inducing ability, but can confer anterior character to more posterior neuroepithelium
    A C Foley
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
    Development 124:2983-96. 1997
    ..The competence of neuroectoderm to respond to anteriorizing signals declines by stages 7-9, but both posteriorizing signals and the ability of neuroectoderm to respond to them persist after this stage...
  67. pmc Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements
    Fang Lin
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Cell Biol 169:777-87. 2005
    ..These findings provide the first evidence that Galpha(12) and Galpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation...
  68. ncbi Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation
    D L Chapman
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, New York, 10032, USA
    Dev Biol 180:534-42. 1996
    ..5 days postcoitus, indicating that the continued expression of Tbx6 is directly or indirectly dependent upon Brachyury expression...
  69. ncbi Specification of cell fates at the dorsal margin of the zebrafish gastrula
    A E Melby
    Institute of Neuroscience, University of Oregon, Eugene, 97405, USA
    Development 122:2225-37. 1996
    ..We define a domain in the early gastrula that is located just at the margin and centered on the dorsal midline, in which most cells generate clones that ..
  70. ncbi Smad2 signaling in extraembryonic tissues determines anterior-posterior polarity of the early mouse embryo
    W R Waldrip
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 92:797-808. 1998
    ..Chimera experiments demonstrate these essential activities are contributed by the extraembryonic tissues. Thus, the extraembryonic tissues play critical roles in establishing the body plan during early mouse development...
  71. pmc FGF/MAPK signaling is required in the gastrula epiblast for avian neural crest induction
    Timothy J Stuhlmiller
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Development 139:289-300. 2012
    ..suggest a later involvement in neural plate border development, likely due to widespread FGF/MAPK activity in the gastrula epiblast...
  72. pmc BMP antagonism protects Nodal signaling in the gastrula to promote the tissue interactions underlying mammalian forebrain and craniofacial patterning
    Yu Ping Yang
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 3709, USA
    Hum Mol Genet 19:3030-42. 2010
    ....
  73. pmc Specification of germ layer identity in the chick gastrula
    Susan C Chapman
    University of Utah School of Medicine, Department of Neurobiology and Anatomy, and Children s Health Research Center, Room 2R066 SOM, 30 North 1900 East, Salt Lake City, Utah 84132 2101, USA
    BMC Dev Biol 7:91. 2007
    ....
  74. ncbi Determination of the zebrafish forebrain: induction and patterning
    Y Grinblat
    Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Development 125:4403-16. 1998
    ..At mid-gastrula, expression of these genes defines a very early pattern in the presumptive neurectoderm, with opl later expressed ..
  75. ncbi Tension-dependent collective cell movements in the early gastrula ectoderm of Xenopus laevis embryos
    L V Beloussov
    Department of Embryology, Faculty of Biology, Moscow State University Moscow 119899 Russia E mail
    Dev Genes Evol 210:92-104. 2000
    Ventral ectodermal explants taken from early gastrula embryos of Xenopus laevis were artificially stretched either by two opposite concentrated forces or by a distributed force applied to the internal explant's layer...
  76. ncbi Retinoic acid signaling targets Hox genes during the amphioxus gastrula stage: insights into early anterior-posterior patterning of the chordate body plan
    Demian Koop
    Marine Biology Research Division, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093 0202, USA
    Dev Biol 338:98-106. 2010
    Previous studies of vertebrate development have shown that retinoic acid (RA) signaling at the gastrula stage strongly influences anterior-posterior (A-P) patterning of the neurula and later stages...
  77. ncbi Axial patterning in cephalochordates and the evolution of the organizer
    Jr Kai Yu
    Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, California 92037 0202, USA
    Nature 445:613-7. 2007
    The organizer of the vertebrate gastrula is an important signalling centre that induces and patterns dorsal axial structures. Although a topic of long-standing interest, the evolutionary origin of the organizer remains unclear...
  78. ncbi Specification of an anterior neuroectoderm patterning by Frizzled8a-mediated Wnt8b signalling during late gastrulation in zebrafish
    Seok Hyung Kim
    Department of Genetic Engineering, Kyungpook National University, Daegu 702 701, Korea
    Development 129:4443-55. 2002
    ..fz8a) gene expressed in the presumptive anterior neuroectoderm as well as prechordal plate at the late gastrula stage. We have investigated the role of Fz8a-mediated Wnt8b signalling in anterior brain patterning in zebrafish...
  79. ncbi Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos
    C Thisse
    Institute of Molecular Biology, University of Oregon, Eugene 97403
    Development 119:1203-15. 1993
    ..The work presented here suggests that snail1 is involved in morphogenetic events during gastrulation, somitogenesis and development of the cephalic neural crest, and that no tail may act as a positive regulator of snail1...
  80. ncbi A novel Cripto-related protein reveals an essential role for EGF-CFCs in Nodal signalling in Xenopus embryos
    Karel Dorey
    Laboratory of Developmental Signalling, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, WC2A 3PX London, UK
    Dev Biol 292:303-16. 2006
    ..We have focused on the role of XCR1/FRL1 and XCR3, which are both expressed at gastrula stages when Nodal signalling is active...
  81. ncbi The role of paraxial protocadherin in selective adhesion and cell movements of the mesoderm during Xenopus gastrulation
    S H Kim
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California, Los Angeles, CA 90095 1662, USA
    Development 125:4681-90. 1998
    ..PAPC may provide a link between regulatory genes in Spemann's organizer and the execution of cell behaviors during morphogenesis...
  82. pmc Evolution of axis specification mechanisms in jawed vertebrates: insights from a chondrichthyan
    Marion Coolen
    Equipe Développement et Evolution des Vertébrés, UMR 6218, Universite d Orleans, Orleans, France
    PLoS ONE 2:e374. 2007
    ..We show that the dogfish displays compelling similarities with amniotes at blastula and early gastrula stages, including the presence of clear homologs of the hypoblast and extraembryonic ectoderm...
  83. ncbi Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation
    Billie A Moore-Scott
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, University of Cincinnati, Cincinnati, Ohio 45229 3039, USA
    Dev Dyn 236:1997-2003. 2007
    ..We have identified 8 molecular markers that are expressed in discrete domains of the gastrula stage endoderm (e7...
  84. ncbi The eed mutation disrupts anterior mesoderm production in mice
    C Faust
    Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106 4955, USA
    Development 121:273-85. 1995
    ..The data presented are discussed in terms of mechanisms for establishment of the eed phenotype, and are consistent with the eed gene product playing an early role in primitive streak formation and/or organization...
  85. ncbi Measuring cell adhesion forces of primary gastrulating cells from zebrafish using atomic force microscopy
    Pierre Henri Puech
    Center of Biotechnology, TU Dresden, Cellular Machines, Tatzberg 49, 01307 Dresden, Germany
    J Cell Sci 118:4199-206. 2005
    ....
  86. ncbi Murine gastrulation requires HNF-4 regulated gene expression in the visceral endoderm: tetraploid rescue of Hnf-4(-/-) embryos
    S A Duncan
    Laboratory of Molecular Cell Biology, The Rockefeller University, New York, New York 10021, USA
    Development 124:279-87. 1997
    ..Furthermore, specific complementation of Hnf-4(-/-) embryos with tetraploid-derived Hnf-4(+/+) VE rescues their early developmental arrest, showing conclusively that a functional VE is mandatory for gastrulation...
  87. ncbi Evolutionary origins of blastoporal expression and organizer activity of the vertebrate gastrula organizer gene lhx1 and its ancient metazoan paralog lhx3
    Yuuri Yasuoka
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Development 136:2005-14. 2009
    Expression of the LIM homeobox gene lhx1 (lim1) is specific to the vertebrate gastrula organizer. Lhx1 functions as a transcriptional regulatory core protein to exert ;organizer' activity in Xenopus embryos...
  88. ncbi A small population of anterior cells patterns the forebrain during zebrafish gastrulation
    C Houart
    Developmental Biology Research Centre, The Randall Institute, Kings College London, UK
    Nature 391:788-92. 1998
    ..This induction depends on signals arising from a group of cells on the dorsal side of the gastrula. This group of cells constitutes the organizer...
  89. ncbi Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis
    V E Foe
    J Cell Sci 61:31-70. 1983
    ..This highly organized cytoplasm may be involved in providing positional information for the important process of nuclear determination that is known to occur during these stages...
  90. ncbi ANR5, an FGF target gene product, regulates gastrulation in Xenopus
    Hyeyoung A Chung
    Division of Morphogenesis, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, 444 8585, Japan
    Curr Biol 17:932-9. 2007
    ..Our findings reveal for the first time that xANR5 acts through Rho to regulate gastrulation and is an important cytoplasmic partner of PAPC, whose cytoplasmic partner was previously unknown...
  91. ncbi Boundaries and functional domains in the animal/vegetal axis of Xenopus gastrula mesoderm
    G Kumano
    Neuroscience Research Institute, University of California, Santa Barbara, California 93106, USA
    Dev Biol 236:465-77. 2001
    Patterning of the Xenopus gastrula marginal zone in the axis running equatorially from the Spemann organizer-the so--called "dorsal/ventral axis"--has been extensively studied...
  92. ncbi Role of p21-activated kinase in cell polarity and directional mesendoderm migration in the Xenopus gastrula
    Martina Nagel
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Dev Dyn 238:1709-26. 2009
    ..However, Pak1 inhibition interferes with the guidance of mesendoderm migration by directional cues residing in the extracellular matrix of the blastocoel roof, and with mesendoderm translocation in the embryo...
  93. ncbi Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation
    J W Ramos
    Department of Cell Biology, University of Virginia, Charlottesville 22908, USA
    Development 122:2873-83. 1996
    ..We suggest that position-specific inductive interactions are likely to represent a novel and general mechanism by which integrin adhesion is modulated throughout development...
  94. pmc Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm
    A Yamamoto
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California, Los Angeles, California 90095 1662, USA
    Development 125:3389-97. 1998
    ..The PAPC structural cell-surface protein may provide a link between regulatory transcription factors and the actual cell biological behaviors that execute morphogenesis during gastrulation...
  95. ncbi Formation and maintenance of the organizer among the vertebrates
    K Joubin
    Department of Genetics and Development and Center for Neurobiology and Behavior, Columbia University, New York, USA
    Int J Dev Biol 45:165-75. 2001
    ..assumed that once the organizer property is assigned to cells at this early stage, it is fixed so that by the gastrula stage, no new cells acquire organizer properties...
  96. ncbi Induction of the prospective neural crest of Xenopus
    R Mayor
    Laboratory of Developmental Biology, National Institute for Medical Research, Mill Hill, London, UK
    Development 121:767-77. 1995
    ..At certain doses, the cranial crest domains are not separated into lateral masses and there is a reduction in the size of the neural plate...
  97. ncbi Chordin is required for the Spemann organizer transplantation phenomenon in Xenopus embryos
    Michael Oelgeschlager
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Dev Cell 4:219-30. 2003
    ..Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation...
  98. ncbi Embryonic XMab21l2 expression is required for gastrulation and subsequent neural development
    G T Lau
    Department of Pathology, Queen Elizabeth Hospital, 30 Gascoigne Road, Hong Kong, Kowloon, China
    Biochem Biophys Res Commun 280:1378-84. 2001
    ..We demonstrated by RNA interference (RNAi), together with other antisense approaches, that XMab21l2 expression is required for the completion of gastrulation and subsequent neural development...
  99. ncbi Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos
    W S Chen
    Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021
    Genes Dev 8:2466-77. 1994
    ..Development of embryonic structures is severely impaired. These results demonstrate that the expression of HNF-4 in the visceral endoderm is essential for embryonic ectoderm survival and normal gastrulation...
  100. ncbi The neural plate specifies somite size in the Xenopus laevis gastrula
    F V Mariani
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Dev Cell 1:115-26. 2001
    ..show here that signaling from the neural plate specifies somite tissue and regulates somite size in the Xenopus gastrula. Ectopic undifferentiated neural tissue induces massive somite expansion at the expense of intermediate and ..
  101. ncbi The hypoblast of the chick embryo positions the primitive streak by antagonizing nodal signaling
    Federica Bertocchini
    Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, United Kingdom
    Dev Cell 3:735-44. 2002
    ..We propose that, during normal development, the primitive streak forms only when the hypoblast is displaced away from the posterior margin by the endoblast, which lacks Cerberus...

Research Grants62

  1. Morphogenesis of mammalian gut endoderm
    Anna Katerina Hadjantonakis; Fiscal Year: 2012
    ..2) The stereotypical congregation of extraembryonic (visceral) endoderm cells around signaling centers of the gastrula stage embryo...
  2. Identification of Mechanically Sensitive Proteins in Early Development
    Douglas W DeSimone; Fiscal Year: 2013
    ..The mesendoderm of Xenopus laevis gastrula stage embryos undergoes a collective form of cell migration that requires both cell-cell cohesion and engagement ..
  3. Identification of Binding Proteins of the Xenopus Morphogenetic Factor Tumorhead
    Edwin E Traverso; Fiscal Year: 2012
    ..Blocking TH's nuclear translocation causes embryonic lethality at the early gastrula transition...
  4. Regulation of call adhesion in Xenopus
    KEN CHO; Fiscal Year: 2009
    ..the following intriguing observations: First, FLRT3 and Rnd1 are coexpressed in the involuting marginal cells of gastrula stage embryos...
  5. GENETIC ANALYSIS OF CONVERGENT EXTENSION IN ZEBRAFISH
    Lilianna Solnica-Krezel; Fiscal Year: 2013
    ..We will ask if gastrula cells require Clasp1 function and have microtubules that are asymmetrically nucleated at the Golgi in a Clasp1-..
  6. DORSAL-VENTRAL PATTERN FORMATION IN THE ZEBRAFISH EMBRYO
    Mary C Mullins; Fiscal Year: 2013
    ..development is to pattern the cells along the dorsoventral (DV) embryonic axis during late blastula and gastrula stages...
  7. Molecular Interactions During Neural Crest Formation
    MARTIN IGNACIO GARCIA-CASTRO; Fiscal Year: 2013
    ..1 is directed to elucidate the specific contributions made by distinct FGF-signaling branches during blastula and gastrula stages, and to establish differential responses and transcriptional effectors of FGF signaling...
  8. Regulation of Planar Cell Polarity in Migrating Cells
    JASON ROBERT JESSEN; Fiscal Year: 2013
    ..provide a new experimental paradigm explaining how PCP signaling coordinates the directed migration of polarized gastrula cell populations...
  9. Mammalian Foregut and Liver Development
    Aaron M Zorn; Fiscal Year: 2012
    ..Our data supports a model where during gastrula and early somite stages secreted Wnt- antagonists in the anterior endoderm establish foregut identity and ..
  10. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2013
    ..The second aim will determine why cell proliferation is tightly regulated in the post-gastrula embryo such that the progenitors are quiescent, and only divide when they first begin to differentiate...
  11. Establishing Left-Right Asymmetry in Vertebrates
    Cammon Arrington; Fiscal Year: 2013
    ..a role for nodal monocilia in the establishment of LR asymmetry, little is known about formation of the late gastrula node. In zebrafish, the node equivalent is Kupffer's vesicle (KV)...
  12. Plasticity and Regulation in Xenopus Anterior-Posterior Patterning
    Margaret S Saha; Fiscal Year: 2013
    ..Previous experiments from our lab have demonstrated that when the presumptive neural plate is removed from a gastrula-stage embryo, rotated 180o, and transplanted back into a host embryo from which the equivalent region was removed,..
  13. SEGMENTATION AND PATTERNING IN DROSOPHILA
    Eric Wieschaus; Fiscal Year: 2000
    ..demonstrated that genes located in the X chromosome are important in the formation of a proper ZA during early gastrula stages...
  14. Systems Analysis of BMP Regulation in Developing Zebrafish Embryos
    DAVID MICHAEL UMULIS; Fiscal Year: 2013
    ..An image-based zebrafish late blastula- gastrula embryo BMP pattern formation model will be developed and tested for multiple alternative mechanisms of BMP ..
  15. Endothelial lineage specification and differentiation in vertebrate embryos
    Suk Won Jin; Fiscal Year: 2013
    ..we previously generated a laser assisted single-cell resolution fate map in the most ventral region of zebrafish gastrula that provided the first detailed distribution pattern of the hemangioblast, a hypothetical common precursor for ..
  16. ANALYSIS OF CELL LINEAGE IN EMBRYOS
    Roger Pedersen; Fiscal Year: 1992
    ..will establish a normal developmental table for this species and refine culture conditions of cleavage and gastrula sages. Cell lineage analysis will be performed using microinjected tracers at cleavage and gastrula stages...
  17. LATERAL LINE SYSTEM--ONTOGENY AND PHYLOGENY
    RICHARD NORTHCUTT; Fiscal Year: 1999
    ..To test this hypothesis axolotl embryos (late gastrula or neurula stages) will be exposed to retinoic acid, which is known to produce transformations in the expression ..
  18. Lineage and induction of ventrolateral mesoderm
    William Smith; Fiscal Year: 2006
    ..In Xenopus, the mesoderm forms from a band of cells around the equator of the early gastrula embryo...
  19. Maternal and Zygotic Regulation of Endoderm Development
    Daniel Kessler; Fiscal Year: 2006
    ..in Xenopus laevis have identified maternal and zygotic genes that establish the endodermal germ layer at the gastrula stage...
  20. Cell Growth and Differentiation in the Vetebrate Embryo
    Elizabeth Lacy; Fiscal Year: 2005
    The defining feature of the mouse gastrula is the primitive streak, a dynamic structure through which epiblast cells ingress to generate nascent mesoderm and endoderm...
  21. The Role of Eg5 in Radial Glial Development in the Zebrafish Spinal Cord
    MICHAEL BARRESI; Fiscal Year: 2009
    ..By combining both a gfap transgenic line driving the expression of GFP with elegant gastrula staged transplantations, The investigator will target clusters of scattered gfap:GFP+ cells into a non-transgenic ..
  22. DEVELOPMENTAL SWITCH IN EMBRYONIC GENE EXPRESSION
    Matthew Andrews; Fiscal Year: 1993
    ..by RNA polymerase III achieve a somatic pattern of expression by repressing selective templates during a gastrula-neurala transition (GNT)...
  23. GENETICS OF MORPHOGENESIS OF VERTEBRATE GASTRULATION
    DONALD KANE; Fiscal Year: 2003
    How do the cells of the gastrula move to form an embryo? The long term objective of the proposed work is to understand the genetic and biomechanical basis for gastrulation in a model vertebrate, the zebrafish...
  24. PATTERNING OF THE FOREBRAIN AND MIDBRAIN
    Eric Weinberg; Fiscal Year: 1999
    ..Two zebrafish homeodomain genes, zOtx1 and zOtx2, which begin to be expressed during mid-gastrula in a sharply defined region that probably corresponds at this stage to the prospective forebrain and midbrain, ..
  25. HISTONE GENES AND THEIR TRANSCRIPTION
    Eric Weinberg; Fiscal Year: 1990
    ..arranged in a tandemly repeated array, are expressed during stages until late blastula but are inactive at the gastrula stage...
  26. CARDIAC REGULATION OF NKX-25 GENE EXPRESSION
    ANDREW LASSAR; Fiscal Year: 2001
    ..principal investigator has identified a cardiac-inducing activity in the anterior lateral plate endoderm of the gastrula-stage avian embryo, which can induce cells from the posterior primitive streak (which normally gives rise to ..
  27. NEURAL INDUCTION--ANALYSIS USING MONOCLONAL ANTIBODIES
    MARCUS JACOBSON; Fiscal Year: 1993
    ..The initial aim is to isolate components of the gastrula that have neural inductive potency which can be used as immunizing material for production of monoclonal ..
  28. PATTERNING OF THE VERTEBRATE NERVOUS SYSTEM
    Kathryn Zimmerman; Fiscal Year: 1999
    ..The restricted expression of these genes indicates that regionalization of the mediolateral axis is initiated at gastrula stages of development, well before the onset of neurulation...
  29. A Functional Screen for Proteins that Alter Cell Fates
    Julie Baker; Fiscal Year: 2005
    ..Although an incredibly rich source of cell signaling, the mouse gastrula has not been used by molecular biologists to mine for molecules...
  30. ALBINO DELETION COMPLEX AND EARLY MOUSE DEVELOPMENT
    Terry Magnuson; Fiscal Year: 2009
    ..Embryo manipulation through chimera production, jpiblast orthotopic transplants and transplantation of the early gastrula organizer will address whether the mutant epiblast is capable of producing and/or responding to axial organizing ..
  31. Origins of Cardiac Neural Crest in the Chick Epiblast
    MAXELLENDE EZIN; Fiscal Year: 2007
    ..Preliminary results suggest that, at gastrula stage 4, cardiac neural crest precursors are not yet segregated from other crest along the anterior posterior ..
  32. ROLE OF EXTRACELLULAR MATRIX IN FROG GASTRULATION
    Kurt Johnson; Fiscal Year: 1980
    In this project, the investigators are working on characterization of ECM extracted from EDTA dissociated gastrula stage amphibian embryos...
  33. CELL MOTILITY AND CELL INTERACTIONS DURING NEURULATION
    Raymond Keller; Fiscal Year: 2001
    ..The combined approach of manipulating adhesion, recording cell motility with video-microscopy, and direct measurement of mechanical properties and forces promise to solve some long standing problems in neural development. ..
  34. FIBRILLIN AND EARLY XENOPUS PATTERNING
    Raymond Keller; Fiscal Year: 2002
    ..Dr. Keller will test specific hypotheses regarding the possible role of fibrillin in establishing the notochord/somite boundary, and in convergence and extension movements in Xenopus during gastrulation. ..
  35. TRAINING PROGRAM IN DEVELOPMENTAL BIOLOGY
    Raymond Keller; Fiscal Year: 2003
    ..Support will be for the fist 3 years of graduate student or post- doctoral study, with the condition of good progress in training, or until individual awards are obtained. ..
  36. GENETIC REGULATION OF SOMITE MORPHOGENESIS IN ZEBRAFISH
    Lilianna Solnica Krezel; Fiscal Year: 2004
    ....
  37. Misregulation of apoptosis in cloned pig embryos
    Heide Schatten; Fiscal Year: 2007
    ..Pilot data from this R03 small grants program research will be used to apply for funding through the R01 mechanism. [unreadable] [unreadable] [unreadable]..
  38. Regulation of Xenopus Embryonic Development by TGFbeta Superfamily Ligands and SM
    MALCOLM R WHITMAN; Fiscal Year: 2010
    ..The study of TGF superfamily ligands provides a common approach, and a common set of molecular tools, with which to understand the regulation of these important physiological processes. ..
  39. Cytoskeletal organization in apicomplexan parasites
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 research will be used to apply for funding through the RO1 mechanism. ..
  40. A Screen For Drugs:Promote Oligodendrocyte Formatio(RMI)
    Bruce Appel; Fiscal Year: 2005
    ..Neural diseases and injuries have devastating impacts on human health. This work will provide a foundation for identifying and investigating genes and drugs that promote neural regeneration and recovery. ..
  41. Mitochondria distribution in cloned pig embryos
    Heide Schatten; Fiscal Year: 2005
    ..Pilot data from this RO3 small grants program research will be used to apply for funding through the RO1 mechanism. ..
  42. ANALYSIS OF PAIR OF RULE GENES IN SEQUENTIAL SEGMENTATIO
    DAVID WEISBLAT; Fiscal Year: 2003
    ..abstract_text> ..
  43. Developmental control of cell polarity in vertebrate embryos.
    John B Wallingford; Fiscal Year: 2010
    ..Together, these experiments will help us to understand how PCP signaling influences such diverse cellular processes as convergent extension and ciliogenesis. ..
  44. POLARIZED CELL SHAPE CHANGES IN XENOPUS GASTRULATION
    Jen Yi Lee; Fiscal Year: 2008
    ..In the long term, I will expand my research into X. tropicalis to enable the use of genetic tools to study vertebrate morphogenesis. [unreadable] [unreadable] [unreadable]..
  45. Spiralian developmental system for the study of multipotency
    Cesar Arenas Mena; Fiscal Year: 2008
    ..The ultimate goal is to manipulate these genes and reprogram in vivo differentiated cells into multipotent stem cells. [unreadable] [unreadable] [unreadable]..
  46. Regulatory Genomics: BAC-GFP Library of Control Genes
    Eric Davidson; Fiscal Year: 2008
    ..Numerous letters from outside investigators attesting interest in this facility are included as an Appendix to this application. ..
  47. Host-microbe interaction in Drosophila gut
    Y Ip; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  48. Cytokinesis and RNA segregation in zebrafish development
    Francisco Pelegri; Fiscal Year: 2007
    ..abstract_text> ..
  49. Sea Urchin Developmental Biology Conference XIV
    DAVID MC CLAY; Fiscal Year: 2002
    ..The concurrent sessions offer valuable opportunities for graduate students and postdocs to gain experience speaking at an international meeting, and the workshops introduce new approaches in these embryos. ..
  50. DORSAL-VENTRAL PATTERN FORMATION IN THE ZEBRAFISH EMBRYO
    Mary Mullins; Fiscal Year: 2007
    ..Thus it is critical to identify and study all the genetic players in this process. ..
  51. COMPARATIVE GENETICS OF SKELETAL DEFECTS
    Richard Harland; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  52. 2008 Basement Membranes Gordon Research Conference
    JEFFREY MINER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  53. Computational Modeling of Developmental Processes
    LARRY TABER; Fiscal Year: 2009
    ..This software will open up new avenues for studying the mechanics of development in the cardiovascular, nervous, and other systems composed of soft tissue. ..
  54. Genetic Analysis of Silurana Tropicalis Development
    Richard Harland; Fiscal Year: 2005
    ..We will carry out a genetic screen, map deletions cytologically, and optimize insertional mutagenesis. ..
  55. Zic3 and the Control of Body Pattern Formation
    STEPHANIE WARE; Fiscal Year: 2005
    ..Through a combination of supervised research, scientific interchange, and selected coursework within this environment, the candidate will obtain the training necessary to transition to an independent investigator. ..
  56. NEURAL TUBE DEFECTS
    DAVID MC CLAY; Fiscal Year: 2005
    ..abstract_text> ..
  57. Mechanisms of Cell/Matrix Interactions in the Kidney
    JEFFREY MINER; Fiscal Year: 2006
    ..Together, the results of these studies will lead to a more complete understanding of cell-matrix interactions in the kidney and could reveal new avenues for diagnosis and treatment of human kidney disease. ..
  58. Twisted Gastrulation Gene in Vertebrate Development
    Chenbei Chang; Fiscal Year: 2007
    ..abstract_text> ..
  59. GERM LAYER SPECIFICATION
    DAVID MC CLAY; Fiscal Year: 2007
    ..This network eventually will enable us to explore how positional cues are produced and displayed for later morphogenetic patterning. ..
  60. Molecular and genetic analysis of sanpodo
    James B Skeath; Fiscal Year: 2010
    ..Such insight should help us understand the etiology of diseases in which this process is de-regulated and design new methods to treat these diseases. ..
  61. Computational and Experimental Study of Early Cardiac Morphogenesis
    LARRY TABER; Fiscal Year: 2009
    ..In addition, the computational model created during the course of our research may someday lead to advanced models of the developing human heart, which is not amenable to direct experimental study. ..
  62. 2006 Gordon Research Conference on Basement Membranes
    JEFFREY MINER; Fiscal Year: 2006
    ..Studies on the genetic analyses of basement membrane functions and the generation of animal models of human basement membrane disorders will be presented. [unreadable] [unreadable] [unreadable] [unreadable]..