mesoderm

Summary

Summary: The middle germ layer of the embryo.

Top Publications

  1. ncbi Epithelial-mesenchymal transitions in development and pathologies
    Jean Paul Thiery
    Unite Mixte de Recherche 144, Centre National Recherche Scientifique, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Curr Opin Cell Biol 15:740-6. 2003
  2. ncbi Epithelial-mesenchymal transitions in tumour progression
    Jean Paul Thiery
    Centre National Recherche Scientifique Unité Mixte Recherche, 144 Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Cancer 2:442-54. 2002
  3. ncbi Complex networks orchestrate epithelial-mesenchymal transitions
    Jean Paul Thiery
    Centre National de la Recherche Scientifique CNRS Unité Mixte Recherche UMR 144 and Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Mol Cell Biol 7:131-42. 2006
  4. ncbi Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits
    Kornelia Polyak
    Department of Medical Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Cancer 9:265-73. 2009
  5. ncbi Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis
    Jing Yang
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Cell 117:927-39. 2004
  6. ncbi Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis
    Jing Yang
    Department of Pharmacology and Pediatrics, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0636, USA
    Dev Cell 14:818-29. 2008
  7. ncbi The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression
    A Cano
    Instituto de Investigaciones Biomedicas, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Cell Biol 2:76-83. 2000
  8. pmc The epithelial-mesenchymal transition generates cells with properties of stem cells
    Sendurai A Mani
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 133:704-15. 2008
  9. ncbi TGF-beta and epithelial-to-mesenchymal transitions
    Jiri Zavadil
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA
    Oncogene 24:5764-74. 2005
  10. pmc The epithelial-mesenchymal transition: new insights in signaling, development, and disease
    Jonathan M Lee
    Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    J Cell Biol 172:973-81. 2006

Detail Information

Publications335 found, 100 shown here

  1. ncbi Epithelial-mesenchymal transitions in development and pathologies
    Jean Paul Thiery
    Unite Mixte de Recherche 144, Centre National Recherche Scientifique, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Curr Opin Cell Biol 15:740-6. 2003
    ..Current studies further emphasise the critical importance of EMT and provide a better molecular and functional definition of mesenchymal cells and how they emerged >500 million years ago as a key event in evolution...
  2. ncbi Epithelial-mesenchymal transitions in tumour progression
    Jean Paul Thiery
    Centre National Recherche Scientifique Unité Mixte Recherche, 144 Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Cancer 2:442-54. 2002
  3. ncbi Complex networks orchestrate epithelial-mesenchymal transitions
    Jean Paul Thiery
    Centre National de la Recherche Scientifique CNRS Unité Mixte Recherche UMR 144 and Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Nat Rev Mol Cell Biol 7:131-42. 2006
    ..Understanding how mesenchymal cells arise from an epithelial default status will also have a strong impact in unravelling the mechanisms that control fibrosis and cancer progression...
  4. ncbi Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits
    Kornelia Polyak
    Department of Medical Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Cancer 9:265-73. 2009
    ....
  5. ncbi Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis
    Jing Yang
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Cell 117:927-39. 2004
    ..These results establish a mechanistic link between Twist, EMT, and tumor metastasis...
  6. ncbi Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis
    Jing Yang
    Department of Pharmacology and Pediatrics, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0636, USA
    Dev Cell 14:818-29. 2008
    ..Studies in both fields are critical for our molecular understanding of cell migration and morphogenesis...
  7. ncbi The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression
    A Cano
    Instituto de Investigaciones Biomedicas, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Cell Biol 2:76-83. 2000
    ..Snail may thus be considered as a marker for malignancy, opening up new avenues for the design of specific anti-invasive drugs...
  8. pmc The epithelial-mesenchymal transition generates cells with properties of stem cells
    Sendurai A Mani
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 133:704-15. 2008
    ..These findings illustrate a direct link between the EMT and the gain of epithelial stem cell properties...
  9. ncbi TGF-beta and epithelial-to-mesenchymal transitions
    Jiri Zavadil
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA
    Oncogene 24:5764-74. 2005
    ..Here we will review the distinct physiological contexts of EMT and the underlying molecular signaling networks controlled by TGF-beta...
  10. pmc The epithelial-mesenchymal transition: new insights in signaling, development, and disease
    Jonathan M Lee
    Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    J Cell Biol 172:973-81. 2006
    ..E.W. Thompson, and D.F. Newgreen. 2005. Cancer Res. 65:5996-6000; Thompson, E.W., D.F. Newgreen, and D. Tarin. 2005. Cancer Res. 65:5991-5995)...
  11. ncbi Molecular requirements for epithelial-mesenchymal transition during tumor progression
    Margit A Huber
    Department of Dermatology, Vienna Medical University, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Curr Opin Cell Biol 17:548-58. 2005
    ..Increasing evidence suggests that EMT plays a specific role in the migration of cells from a primary tumor into the circulation and may provide a rationale for developing more effective cancer therapies...
  12. ncbi Epithelial-mesenchymal transitions: twist in development and metastasis
    Yibin Kang
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Cell 118:277-9. 2004
    ..A recent report in Cell (Yang et al., 2004) adds Twist to this list and links EMT to the ability of breast cancer cells to enter the circulation and seed metastases...
  13. pmc Evidence that fibroblasts derive from epithelium during tissue fibrosis
    Masayuki Iwano
    Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA
    J Clin Invest 110:341-50. 2002
    ..As a general mechanism, this change in fate highlights the potential plasticity of differentiated cells in adult tissues under pathologic conditions...
  14. ncbi Epithelial-mesenchymal transition in development and cancer: role of phosphatidylinositol 3' kinase/AKT pathways
    Lionel Larue
    Developmental Genetics of Melanocytes, UMR 146, CNRS, Institut Curie, Centre Universitaire, Orsay, France
    Oncogene 24:7443-54. 2005
    ..Interactions between PI3K/AKT and other EMT-inducing pathways are presented, along with a discussion of the therapeutic implications of modulating EMT in order to achieve cancer control...
  15. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
    ..Controlling Wnt/beta-catenin signaling is a common molecular mechanism underlying chondrocyte and osteoblast differentiation and specification of intramembranous and endochondral ossification...
  16. pmc Epithelial-mesenchymal transition and its implications for fibrosis
    Raghu Kalluri
    Center for Matrix Biology, Beth Israel Deaconess Medical Center, 330 Brookline Ave DANA 514, Boston, Massachusetts 02215, USA
    J Clin Invest 112:1776-84. 2003
    ..This review highlights recent advances in the process of EMT signaling in health and disease and how it may be attenuated or reversed by selective cytokines and growth factors...
  17. ncbi Epithelial--mesenchymal and mesenchymal--epithelial transitions in carcinoma progression
    Honor Hugo
    Embryology Laboratory, Murdoch Children s Research Institute, The Royal Children s Hospital, Victoria, Australia
    J Cell Physiol 213:374-83. 2007
    ..Finally, perspectives from a clinical and translational viewpoint are discussed...
  18. ncbi Combinatorial binding predicts spatio-temporal cis-regulatory activity
    Robert P Zinzen
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Nature 462:65-70. 2009
    ..atlas of cis-regulatory modules describing their temporal and combinatorial occupancy during Drosophila mesoderm development...
  19. ncbi Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart
    Chen Leng Cai
    Institute of Molecular Medicine, Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Dev Cell 5:877-89. 2003
    ..Our results have implications for the development of specific cardiac lineages, left-right asymmetry, cardiac evolution, and isolation of cardiac progenitor cells...
  20. pmc Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis
    Katarina Wolf
    Department of Dermatology, University of Wurzburg, 97080 Wurzburg, Germany
    J Cell Biol 160:267-77. 2003
    ....
  21. ncbi Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis
    Jason J Christiansen
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Cancer Res 66:8319-26. 2006
    ..We suggest that these mechanisms can manifest in a series of independent and reversible steps and that EMT represents just one mechanism in the global metastatic carcinoma development process...
  22. ncbi Endothelial-to-mesenchymal transition contributes to cardiac fibrosis
    Elisabeth M Zeisberg
    Division of Matrix Biology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nat Med 13:952-61. 2007
    ..Our findings show that EndMT contributes to the progression of cardiac fibrosis and that rhBMP-7 can be used to inhibit EndMT and to intervene in the progression of chronic heart disease associated with fibrosis...
  23. pmc Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis
    Brigham C Willis
    Department of Anesthesiology Critical Care Medicine, Childrens Hospital Los Angeles, California, USA
    Am J Pathol 166:1321-32. 2005
    ..These results suggest that AECs undergo EMT when chronically exposed to TGF-beta1, raising the possibility that epithelial cells may serve as a novel source of myofibroblasts in IPF...
  24. pmc UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity
    Chaochen Wang
    Laboratory of Endocrinology and Receptor Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:15324-9. 2012
    ..However, UTX KO cells show severe defects in mesoderm differentiation and induction of Brachyury, a transcription factor essential for mesoderm development...
  25. pmc NF-kappaB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression
    Margit A Huber
    Department of Physiological Chemistry, Ulm University, Albert Einstein Allee 11, D 89081 Ulm, Germany
    J Clin Invest 114:569-81. 2004
    ....
  26. ncbi Induction of pancreatic differentiation by signals from blood vessels
    E Lammert
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Science 294:564-7. 2001
    ..These results indicate that vessels not only provide metabolic sustenance, but also provide inductive signals for organ development...
  27. ncbi Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition
    Masato Maeda
    Department of Oral Biology College of Dentistry, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    J Cell Sci 118:873-87. 2005
    ..Together, these data suggest that cadherin switching is necessary for increased motility but is not required for the morphological changes that accompany EMT...
  28. pmc Targeted disruption of TGF-beta1/Smad3 signaling protects against renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction
    Misako Sato
    Department of Pathology, Wakayama Medical University, Wakayama, Japan
    J Clin Invest 112:1486-94. 2003
    ..Together the data demonstrate that the Smad3 pathway is central to the pathogenesis of interstitial fibrosis and suggest that inhibitors of this pathway may have clinical application in the treatment of obstructive nephropathy...
  29. ncbi Invasion and metastasis in colorectal cancer: epithelial-mesenchymal transition, mesenchymal-epithelial transition, stem cells and beta-catenin
    Thomas Brabletz
    Department of Pathology, University of Erlangen Nurnberg, Erlangen, Germany
    Cells Tissues Organs 179:56-65. 2005
    ..Accumulating data demonstrate that aberrant nuclear expression of beta-catenin can also confer these two abilities to tumor cells, thereby driving malignant tumor progression...
  30. ncbi Multipotent mesenchymal stem cells from adult human synovial membrane
    C De Bari
    Katholieke Universiteit Leuven, Belgium
    Arthritis Rheum 44:1928-42. 2001
    ..SM-derived MSCs may play a role in the regenerative response during arthritic diseases and are promising candidates for developing novel cell-based therapeutic approaches for postnatal skeletal tissue repair...
  31. ncbi Tissue origins and interactions in the mammalian skull vault
    Xiaobing Jiang
    Institute for Genetic Medicine, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    Dev Biol 241:106-16. 2002
    ..permanent neural crest cell lineage marker, Wnt1-Cre/R26R, we show that both sutures are formed at a neural crest-mesoderm interface: the frontal bones are neural crest-derived and the parietal bones mesodermal, with a tongue of neural ..
  32. ncbi Epithelial to mesenchymal transition is a determinant of sensitivity of non-small-cell lung carcinoma cell lines and xenografts to epidermal growth factor receptor inhibition
    Stuart Thomson
    Departments of Translational Research and Oncology Research, OSI Pharmaceuticals, Inc, Farmingdale, NY 11735, USA
    Cancer Res 65:9455-62. 2005
    ..These data suggest that EMT may be a general biological switch rendering non-small cell lung tumors sensitive or insensitive to EGFR inhibition...
  33. ncbi Epithelial versus mesenchymal phenotype determines in vitro sensitivity and predicts clinical activity of erlotinib in lung cancer patients
    Robert L Yauch
    Department of Molecular Diagnostics, Genentech, Inc, South San Francisco, California 94080, USA
    Clin Cancer Res 11:8686-98. 2005
    ..These data support a potential role for EMT as a determinant of EGFR activity in NSCLC tumor cells and E-cadherin expression as a novel biomarker predicting clinical activity of the EGFR inhibitor erlotinib in NSCLC patients...
  34. ncbi The protein kinase Akt induces epithelial mesenchymal transition and promotes enhanced motility and invasiveness of squamous cell carcinoma lines
    Sylvia Julien Grille
    Developmental Genetics of Melanocytes, UMR 146 CNRS Institut Curie, 91405 Orsay Cedex, France
    Cancer Res 63:2172-8. 2003
    ..These findings suggest that future therapies based on AKT inhibition may complement conventional treatments by controlling tumor cell invasion and metastasis...
  35. ncbi Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition
    Binhua P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 6:931-40. 2004
    ..Furthermore, inhibition of GSK-3beta results in the upregulation of Snail and downregulation of E-cadherin in vivo. Thus, Snail and GSK-3beta together function as a molecular switch for many signalling pathways that lead to EMT...
  36. pmc A machine learning approach for identifying novel cell type-specific transcriptional regulators of myogenesis
    Brian W Busser
    Laboratory of Developmental Systems Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Genet 8:e1002531. 2012
    ....
  37. pmc The mouse snail gene encodes a key regulator of the epithelial-mesenchymal transition
    E A Carver
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Mol Cell Biol 21:8184-8. 2001
    ..Mouse embryos deficient for the Snail (Sna) gene exhibit defects in the formation of the mesoderm germ layer...
  38. ncbi Development of definitive endoderm from embryonic stem cells in culture
    Atsushi Kubo
    The Carl C Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 131:1651-62. 2004
    ..to the brachyury locus, we demonstrate that endoderm develops from a brachyury(+) population that also displays mesoderm potential...
  39. pmc A novel lung metastasis signature links Wnt signaling with cancer cell self-renewal and epithelial-mesenchymal transition in basal-like breast cancer
    Theresa A DiMeo
    Department of Anatomy and Cellular Biology, Sackler School, Tufts University School of Medicine, Boston, MA 02111, USA
    Cancer Res 69:5364-73. 2009
    ..Collectively, these results provide a molecular link between self-renewal, EMT, and metastasis in basal-like breast cancers...
  40. pmc Epithelial-mesenchymal transition: a cancer researcher's conceptual friend and foe
    Michael W Klymkowsky
    Department of Molecular, Cellular, and Development Biology, University of Colorado, Boulder, Colorado, USA
    Am J Pathol 174:1588-93. 2009
    ....
  41. ncbi Control of cell behavior during vertebrate development by Slug, a zinc finger gene
    M A Nieto
    Instituto Cajal, Madrid, Spain
    Science 264:835-9. 1994
    ..This change, which is the transition from epithelial to mesenchymal character, occurs at the formation of mesoderm during gastrulation and on emigration of the neutral crest from the neural tube.
  42. ncbi Transforming growth factor beta-1 induces snail transcription factor in epithelial cell lines: mechanisms for epithelial mesenchymal transitions
    Hector Peinado
    Instituto de Investigaciones Biomédicas Alberto Sols CSIC UAM and Departamento de Bioquímica UAM, Arturo Duperier 4, 28029 Madrid, Spain
    J Biol Chem 278:21113-23. 2003
    ..These data support that MAPK and phosphatidylinositol 3-kinase signaling pathways are implicated in TGFbeta1-mediated induction of Snail promoter, probably through Ras activation and its downstream effectors...
  43. ncbi BMP-7 counteracts TGF-beta1-induced epithelial-to-mesenchymal transition and reverses chronic renal injury
    Michael Zeisberg
    Center for Matrix Biology, Gastroenterology and Renal Divisions, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nat Med 9:964-8. 2003
    ..Collectively, these results provide evidence of cross talk between BMP-7 and TGF-beta1 in the regulation of EMT in health and disease...
  44. pmc Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition
    Hanna Y Irie
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 171:1023-34. 2005
    ..These results highlight the distinct functions of Akt isoforms in regulating growth factor-stimulated EMT and cell migration, as well as the importance of Akt1 in cross-regulating the ERK signaling pathway...
  45. ncbi Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung
    S Bellusci
    Department of Cell Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2175, USA
    Development 124:4867-78. 1997
    ..We conclude that, during early lung development, localized sources of FGF10 in the mesoderm regulate endoderm proliferation and bud outgrowth.
  46. ncbi Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition
    Michael Zeisberg
    Division of Matrix Biology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachussetts 02215, USA
    J Biol Chem 282:23337-47. 2007
    ..Furthermore, our findings suggest that EMT is a promising therapeutic target for the attenuation of liver fibrosis...
  47. ncbi Leaving the neighborhood: molecular mechanisms involved during epithelial-mesenchymal transition
    P Savagner
    Equipe Genome et Cancer, UMR CNRS 5535, CRLC Val d Aurelle Paul Larmarque, 34298 Montpellier, Cedex 5, France
    Bioessays 23:912-23. 2001
    ..Recent functional and localization experiments indicate that the slug/snail family of transcription factors functions overall as an epithelial phenotype repressor and could represent a key EMT contributor...
  48. ncbi A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo
    T P Yamaguchi
    Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, MA 02138, USA
    Development 126:1211-23. 1999
    ..The reduced number of proliferating cells in both the progress zone and the primitive streak mesoderm suggests that one function of Wnt5a is to regulate the proliferation of progenitor cells.
  49. pmc Alveolar epithelial cell mesenchymal transition develops in vivo during pulmonary fibrosis and is regulated by the extracellular matrix
    Kevin K Kim
    Department of Medicine and Cardiovascular Research Institute, University of California, San Francisco, 94143, USA
    Proc Natl Acad Sci U S A 103:13180-5. 2006
    ..These data reveal alveolar epithelial cells as progenitors for fibroblasts in vivo and implicate the provisional extracellular matrix as a key regulator of epithelial transdifferentiation during fibrogenesis...
  50. pmc Rac1b and reactive oxygen species mediate MMP-3-induced EMT and genomic instability
    Derek C Radisky
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nature 436:123-7. 2005
    ..These findings identify a previously undescribed pathway in which a component of the breast tumour microenvironment alters cellular structure in culture and tissue structure in vivo, leading to malignant transformation...
  51. ncbi Haemangioblast commitment is initiated in the primitive streak of the mouse embryo
    Tara L Huber
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York City, New York 10029, USA
    Nature 432:625-30. 2004
    ..fluorescent protein targeted to the brachyury locus demonstrates that the haemangioblast is a subpopulation of mesoderm that co-expresses brachyury (also known as T) and Flk-1 (also known as Kdr)...
  52. pmc Fibroblasts in kidney fibrosis emerge via endothelial-to-mesenchymal transition
    Elisabeth M Zeisberg
    Division of Matrix Biology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    J Am Soc Nephrol 19:2282-7. 2008
    ..Collectively, our results demonstrate that EndMT contributes to the accumulation of activated fibroblasts and myofibroblasts in kidney fibrosis and suggest that targeting EndMT might have therapeutic potential...
  53. pmc Notch promotes epithelial-mesenchymal transition during cardiac development and oncogenic transformation
    Luika A Timmerman
    University of California Comprehensive Cancer Center, San Francisco, California 94115, USA
    Genes Dev 18:99-115. 2004
    ..Notch is expressed in embryonic regions where EMT occurs, suggesting an intimate and fundamental role for Notch, which may be reactivated during tumor metastasis...
  54. ncbi Fibroblast growth factor signaling during early vertebrate development
    Ralph T Böttcher
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, D 69120 Heidelberg, Germany
    Endocr Rev 26:63-77. 2005
    ..We focus on FGF signaling during early embryonic processes in vertebrates, such as induction and patterning of the three germ layers as well as its function in the control of morphogenetic movements...
  55. pmc TGFbeta2 knockout mice have multiple developmental defects that are non-overlapping with other TGFbeta knockout phenotypes
    L P Sanford
    Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, OH 45267, USA
    Development 124:2659-70. 1997
    ..There is no phenotypic overlap with TGFbeta1- and TGFbeta3-null mice indicating numerous non-compensated functions between the TGFbeta isoforms...
  56. pmc Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors
    Jun K Takeuchi
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Nature 459:708-11. 2009
    ..Here we define the minimal requirements for transdifferentiation of mouse mesoderm to cardiac myocytes...
  57. ncbi Recent advances in craniofacial morphogenesis
    Yang Chai
    Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, California 90033, USA
    Dev Dyn 235:2353-75. 2006
    ..Together, these findings will be beneficial for the understanding, treatment, and prevention of human congenital malformations and establish the foundation for craniofacial tissue regeneration...
  58. ncbi Transcriptional regulation of cell polarity in EMT and cancer
    G Moreno-Bueno
    Departamento de Bioquimica, Universidad Autonoma de Madrid, Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Madrid, Spain
    Oncogene 27:6958-69. 2008
    ..These recent observations provide new insights into the relationship between alterations in cell polarity components and EMT in cancer, opening new avenues for their potential use as therapeutic targets to prevent tumour progression...
  59. ncbi Transforming growth factor-beta signaling during epithelial-mesenchymal transformation: implications for embryogenesis and tumor metastasis
    Ali Nawshad
    Department of Oral Biology, College of Dentistry, University of Nebraska Medical Center, Lincoln, NE 68583, USA
    Cells Tissues Organs 179:11-23. 2005
    ..Here we assess the biochemical signaling pathways of TGF-beta and their potential cross-interaction with traditional Wnt signaling molecules to bring about EMT during embryogenesis and tumor metastasis...
  60. ncbi The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo
    S Schulte-Merker
    Abteilung Genetik, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Development 116:1021-32. 1992
    Embryos mutant for the T gene, in mice, make insufficient mesoderm and fail to develop a notochord...
  61. pmc Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme
    T D Camenisch
    Samuel C Johnson Medical Research Center, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Clin Invest 106:349-60. 2000
    ..They also reveal a previously unrecognized pathway for cell migration and invasion that is HA-dependent and involves Ras activation...
  62. ncbi Cloning of the T gene required in mesoderm formation in the mouse
    B G Herrmann
    Laboratory of Molecular Embryology, National Institute for Medical Research, London, UK
    Nature 343:617-22. 1990
    The murine developmental mutation T identifies an essential gene in mesoderm formation. Embryos lacking normal gene activity fail to form the notochord, the entire posterior region and the allantois, and die at about 10 days of gestation...
  63. ncbi Gene expression profiling of breast cell lines identifies potential new basal markers
    E Charafe-Jauffret
    Marseille Cancer Institute, Laboratory of Molecular Oncology, Inserm Institut Paoli Calmettes, Marseille, France
    Oncogene 25:2273-84. 2006
    ....
  64. ncbi The epithelial-to-mesenchymal transition and cancer stem cells: a coalition against cancer therapies
    Brett G Hollier
    Department of Molecular Pathology, University of Texas M D Anderson Cancer Center, Houston, TX 77054, USA
    J Mammary Gland Biol Neoplasia 14:29-43. 2009
    ..The purpose of this review is to summarize the findings related to EMT and stem cells in cancer progression and therapy resistance...
  65. pmc A mesoderm-derived precursor for mesenchymal stem and endothelial cells
    Maxim A Vodyanik
    National Primate Research Center, University of Wisconsin Graduate School, Madison, 53715, USA
    Cell Stem Cell 7:718-29. 2010
    Among the three embryonic germ layers, the mesoderm is a major source of the mesenchymal precursors giving rise to skeletal and connective tissues, but these precursors have not previously been identified and characterized...
  66. ncbi Chronic oxaliplatin resistance induces epithelial-to-mesenchymal transition in colorectal cancer cell lines
    Anthony D Yang
    Department of Surgical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77230 1402, USA
    Clin Cancer Res 12:4147-53. 2006
    ..In preliminary studies, we observed that oxaliplatin-resistant (OxR) colorectal cancer (CRC) cells underwent morphologic changes suggestive of a migratory phenotype, leading us to hypothesize that OxR CRC cells undergo EMT...
  67. ncbi Actions of TGF-beta as tumor suppressor and pro-metastatic factor in human cancer
    Katerina Pardali
    Ludwig Institute for Cancer Research, Box 595 Biomedical Center, Uppsala University, SE 751 24 Uppsala, Sweden
    Biochim Biophys Acta 1775:21-62. 2007
    ..In conclusion, TGF-beta signaling is intimately implicated in tumor development and contributes to all cardinal features of tumor cell biology...
  68. ncbi Activation of NF-kappaB by Akt upregulates Snail expression and induces epithelium mesenchyme transition
    S Julien
    1Developmental Genetics of Melanocytes, UMR 146 CNRS Institut Curie, Bat 110, Orsay Cedex, France
    Oncogene 26:7445-56. 2007
    ..Thus, this signalling and transcriptional network linking AKT, NF-kappaB, Snail and E-cadherin during EMT is a potential target for antimetastatic therapeutics...
  69. ncbi Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages
    S I Nishikawa
    Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan
    Development 125:1747-57. 1998
    ..Here we demonstrate that ES cells can differentiate to hemopoietic cells through the proximal lateral mesoderm, merely upon culturing in type IV collagen-coated dishes...
  70. pmc Cell size and invasion in TGF-beta-induced epithelial to mesenchymal transition is regulated by activation of the mTOR pathway
    Samy Lamouille
    Department of Cell and Tissue Biology, Program in Cell Biology, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 178:437-51. 2007
    ..Activation of mTOR by TGF-beta, which leads to increased cell size and invasion, adds to the role of TGF-beta-induced EMT in cancer progression and may represent a therapeutic opportunity for rapamycin analogues in cancer...
  71. ncbi Epithelial-mesenchymal interconversions in normal ovarian surface epithelium and ovarian carcinomas: an exception to the norm
    Nuzhat Ahmed
    Women s Cancer Research Centre, Royal Women s Hospital, Melbourne, Victoria, Australia
    J Cell Physiol 213:581-8. 2007
    ..Finally, emphasis is placed on the clinical implications of EMT and potential therapeutic opportunities that may arise from these observations have been proposed...
  72. pmc uPAR induces epithelial-mesenchymal transition in hypoxic breast cancer cells
    Robin D Lester
    Department of Pathology, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 178:425-36. 2007
    ..We conclude that in hypoxia, uPAR activates diverse cell signaling pathways that cooperatively induce EMT and may promote cancer metastasis...
  73. ncbi Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin
    E L George
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139
    Development 119:1079-91. 1993
    ..The nature of these embryonic defects leads to reevaluation of suggested roles for fibronectin during early development based on results obtained in vitro and in embryos of other species...
  74. ncbi MicroRNAs as regulators of epithelial-mesenchymal transition
    Philip A Gregory
    Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia
    Cell Cycle 7:3112-8. 2008
    ..This review summarises these recent findings and their implications in both developmental EMT and tumor progression...
  75. ncbi NF-kappaB and epithelial to mesenchymal transition of cancer
    Chengyin Min
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 2394, USA
    J Cell Biochem 104:733-44. 2008
    ..Overall, NF-kappaB is identified as a key target in prevention and in the treatment of invasive carcinomas...
  76. ncbi Abnormal lung development and cleft palate in mice lacking TGF-beta 3 indicates defects of epithelial-mesenchymal interaction
    V Kaartinen
    Department of Pathology, Childrens Hospital of Los Angeles Research Institute, California 90027, USA
    Nat Genet 11:415-21. 1995
    ..This study demonstrates an essential function for TGF-beta 3 in the normal morphogenesis of palate and lung, and directly implicates this cytokine in mechanisms of epithelial-mesenchymal interaction...
  77. ncbi TGF-beta-induced EMT: mechanisms and implications for fibrotic lung disease
    Brigham C Willis
    Heart and Lung Institute, St Joseph s Hospital and Medical Center, Phoenix, Arizona, USA
    Am J Physiol Lung Cell Mol Physiol 293:L525-34. 2007
    ....
  78. ncbi Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts
    Elisabeth M Zeisberg
    Division of Matrix Biology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
    Cancer Res 67:10123-8. 2007
    ....
  79. pmc Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition
    Jiri Zavadil
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA
    EMBO J 23:1155-65. 2004
    ....
  80. pmc Epidermal growth factor receptor cooperates with signal transducer and activator of transcription 3 to induce epithelial-mesenchymal transition in cancer cells via up-regulation of TWIST gene expression
    Hui Wen Lo
    Department of Surgery, The Comprehensive Cancer Center, Duke University, Durham, North Carolina, USA
    Cancer Res 67:9066-76. 2007
    ..Together, we report here that EGF/EGFR signaling pathways induce cancer cell EMT via STAT3-mediated TWIST gene expression...
  81. pmc The polycomb group protein Bmi-1 represses the tumor suppressor PTEN and induces epithelial-mesenchymal transition in human nasopharyngeal epithelial cells
    Li Bing Song
    State Key Laboratory of Oncology in South China, Sun Yat sen University Cancer Center, Guangzhou, People s Republic of China
    J Clin Invest 119:3626-36. 2009
    ..Our results provide functional and mechanistic links between the oncoprotein Bmi-1 and the tumor suppressor PTEN in the development and progression of cancer...
  82. ncbi Msx1 deficient mice exhibit cleft palate and abnormalities of craniofacial and tooth development
    I Satokata
    Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115
    Nat Genet 6:348-56. 1994
    ..The Msx1-/Msx1- phenotype is similar to human cleft palate, and provides a genetic model for cleft palate and oligodontia in which the defective gene is known...
  83. ncbi Investigating the origins of triploblasty: 'mesodermal' gene expression in a diploblastic animal, the sea anemone Nematostella vectensis (phylum, Cnidaria; class, Anthozoa)
    Mark Q Martindale
    Kewalo Marine Laboratory, Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI 96813, USA
    Development 131:2463-74. 2004
    b>Mesoderm played a crucial role in the radiation of the triploblastic Bilateria, permitting the evolution of larger and more complex body plans than in the diploblastic, non-bilaterian animals...
  84. ncbi Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow
    A M Mackay
    Osiris Therapeutics, Inc, Baltimore, Maryland 21231 2001, USA
    Tissue Eng 4:415-28. 1998
    ..Increased understanding of the induction of chondrogenic differentiation should lead to further progress in defining the mechanisms responsible for the generation of cartilaginous tissues, their maintenance, and their regeneration...
  85. pmc FGF2 sustains NANOG and switches the outcome of BMP4-induced human embryonic stem cell differentiation
    Pengzhi Yu
    Morgridge Institute for Research, Madison, WI 53715 7365, USA
    Cell Stem Cell 8:326-34. 2011
    ..Together, our results demonstrate that FGF2 signaling switches the outcome of BMP4-induced differentiation of human ESCs by maintaining NANOG levels through the MEK-ERK pathway...
  86. pmc Mapping the first stages of mesoderm commitment during differentiation of human embryonic stem cells
    Denis Evseenko
    Department of Pathology and Laboratory Medicine, and Broad Stem Cell Research Center and Cardiovascular Research Laboratory, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 107:13742-7. 2010
    ..of how mesodermal tissue is formed has been limited by the absence of specific and reliable markers of early mesoderm commitment...
  87. pmc Lysosomal targeting of E-cadherin: a unique mechanism for the down-regulation of cell-cell adhesion during epithelial to mesenchymal transitions
    Felipe Palacios
    Department of Biological Sciences, University of Notre Dame, Box 369, Galvin Life Sciences Bldg, Notre Dame, IN 46556 0369, USA
    Mol Cell Biol 25:389-402. 2005
    ..Our studies reveal that the lysosomal targeting of E-cadherin is an important posttranscriptional mechanism to deplete cellular E-cadherin during Src-induced epithelial to mesenchymal transitions...
  88. ncbi Hormonal, cellular, and molecular regulation of normal and neoplastic prostatic development
    Gerald R Cunha
    Department of Anatomy, University of California, Box 0452, 513 Parnassus Avenue, San Francisco, CA 94143 0452, USA
    J Steroid Biochem Mol Biol 92:221-36. 2004
    ..Thus, stromal-epithelial interactions play critical roles in the hormonal, cellular, and molecular regulation of normal and neoplastic prostatic development...
  89. ncbi In vitro modeling of paraxial and lateral mesoderm differentiation reveals early reversibility
    Hidetoshi Sakurai
    Laboratory for Stem Cell Biology, RIKEN Center for Development Biology, 2 2 3 Minatojimaminamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Stem Cells 24:575-86. 2006
    ..ECs) are thought to be derived mainly from the vascular endothelial growth factor receptor 2 (VEGFR-2)+ lateral mesoderm during early embryogenesis...
  90. ncbi PRL-3 down-regulates PTEN expression and signals through PI3K to promote epithelial-mesenchymal transition
    Haihe Wang
    Institute of Molecular and Cell Biology, Singapore, Singapore
    Cancer Res 67:2922-6. 2007
    ..Targeting PRL-3 may thus be a useful strategy to impede cancer cell invasion and metastasis...
  91. pmc Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation
    Benjamin L Martin
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Dev Cell 15:121-33. 2008
    ..We propose that a positive autoregulatory loop between Ntl/Bra and canonical Wnt signaling maintains the mesodermal progenitors to facilitate posterior somite development in chordates...
  92. ncbi Pluripotency of mesenchymal stem cells derived from adult marrow
    Yuehua Jiang
    Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Nature 418:41-9. 2002
    ..or MAPCs--differentiate, at the single cell level, not only into mesenchymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro...
  93. ncbi E-cadherin, beta-catenin, and ZEB1 in malignant progression of cancer
    Otto Schmalhofer
    Department of Visceral Surgery, University of Freiburg, Hugstetter Strasse 55, 79106, Freiburg, Germany
    Cancer Metastasis Rev 28:151-66. 2009
    ....
  94. pmc Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients
    Bahriye Aktas
    Department of Gynecology and Obstetrics, University Hospital Essen, University of Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany
    Breast Cancer Res 11:R46. 2009
    ....
  95. pmc Generation of breast cancer stem cells through epithelial-mesenchymal transition
    Anne Pierre Morel
    Centre Leon Berard, Lyon, France
    PLoS ONE 3:e2888. 2008
    ..The acquisition of these stem and tumorigenic characters is driven by EMT induction...
  96. ncbi Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail
    Junichi Ikenouchi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Sci 116:1959-67. 2003
    ..e. the expression of adherens and tight junction adhesion molecules, respectively)...
  97. ncbi NF-kappaB represses E-cadherin expression and enhances epithelial to mesenchymal transition of mammary epithelial cells: potential involvement of ZEB-1 and ZEB-2
    H L Chua
    Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA
    Oncogene 26:711-24. 2007
    ..MCF10A cells chronically exposed to tumor necrosis factor alpha (TNFalpha), a potent NF-kappaB inducer, also exhibited the EMT-like phenotype and ZEB-1/ZEB-2 induction, both of which were reversed following TNFalpha withdrawal...
  98. ncbi The role of epithelial-to-mesenchymal transition in renal fibrosis
    Michael Zeisberg
    Center for Matrix Biology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA
    J Mol Med (Berl) 82:175-81. 2004
    ..Such antagonism results in the repair of injured kidneys, suggesting that modulation of epithelial cell plasticity has therapeutic advantages...
  99. ncbi Novel STAT3 target genes exert distinct roles in the inhibition of mesoderm and endoderm differentiation in cooperation with Nanog
    Pierre Yves Bourillot
    Institut National de la Sante et de la Recherche Medicale, U846, Bron, France
    Stem Cells 27:1760-71. 2009
    ..How the LIF/STAT3 pathway inhibits commitment to both mesoderm and endoderm lineages is presently unknown...
  100. ncbi Linking epithelial-mesenchymal transition to the well-known polarity protein Par6
    Jean Paul Thiery
    CNRS UMR 144, Translational Research Department, Institut Curie, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Dev Cell 8:456-8. 2005
    ..This new study provides further insight into the protein networks involved in topologically regulated control of epithelial cell polarity and plasticity...
  101. ncbi Statin-triggered cell death in primary human lung mesenchymal cells involves p53-PUMA and release of Smac and Omi but not cytochrome c
    Saeid Ghavami
    Department of Physiology, University of Manitoba, Winnipeg, MB, Canada
    Biochim Biophys Acta 1803:452-67. 2010
    ..Thus, simvastatin activates novel apoptosis pathways in lung mesenchymal cells involving p53, IAP inhibitor release, and disruption of mitochondrial fission...

Research Grants69

  1. MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION
    Michael R Green; Fiscal Year: 2013
    ..identified a single TRF3 target gene, mespa, which is required for early embryonic development and commitment of mesoderm to the hematopoietic lineage...
  2. BMP signaling in vertebrate development
    Ken W Y Cho; Fiscal Year: 2013
    ..2. BRE reporter gene experiments in Xenopus reveal that BMP signaling is not only active in the mesoderm and ectoderm, as is well known, but also in the endoderm 3...
  3. MECHANISMS OF FGF RECEPTOR REGULATION AND SIGNALING
    Moosa Mohammadi; Fiscal Year: 2013
    ..The paracrine FGF1, FGF4, FGF7, FGF8, and FGF9 subfamilies play essential roles in spermatogenesis, mesoderm induction, somitogenesis, organogenesis, and pattern formation, whereas the FGF19 subfamily acts in an endocrine ..
  4. Novel Collagen II Alternative Transcripts and Mouse Skeletal Development
    Audrey McAlinden; Fiscal Year: 2010
    ..low levels during development in a number of embryonic non-cartilage tissues such as the notochord, somitic mesoderm, fetal brain, spinal cord, developing eye, tendons and ligaments...
  5. Use of umbilical cord as a unique stem cell source for tissue regeneration
    Xiao Dong Chen; Fiscal Year: 2013
    ..commitment to a specific cell lineage, including osteoblasts, adipocytes, chondroblasts and cardiomyocytes (mesoderm), nerve (ectoderm) and hepatocytes (endoderm)...
  6. A Cis-regulatory Model for Neural Border Induction
    Daniel Meulemans Medeiros; Fiscal Year: 2013
    ..process has been shown to involve Wnt and BMP signals secreted from adjacent ectoderm and underlying paraxial mesoderm. These signals induce neural border formation by activating the expression of the 'neural border specifier&..
  7. INDUCIBLE PLURIPOTENT STEM CELLS AND KIDNEY REGENERATION
    JOSEPH VINCENT BONVENTRE; Fiscal Year: 2010
    ..will signify the successful differentiation of mouse ES cells into renal progenitor cells from the intermediate mesoderm, the precursor to all kidney tissue, and the metanephric mesenchyme, respectively...
  8. Regulation of TGF-beta Signaling and Embryonic Development by GTPases
    Gerald H Thomsen; Fiscal Year: 2010
    ..Blocking endogenous GTPBP 1 or 2 in developing embryos with antisense oligonucleotides disrupts mesoderm differentiation, body patterning and organogenesis...
  9. There Will Be Blood: Stem Cell Niche Driven Derivation of HSC from ES Cells
    PAUL JOHN SIMMONS; Fiscal Year: 2010
    ..Thus following their specification and emergence from primitive mesoderm, the subsequent proliferation and maintenance of HSC occurs in association with a series of discrete ..
  10. Role of the zinc finger transcription factor ZBP89 in blood &vascular developmen
    M Amin Arnaout; Fiscal Year: 2013
    ..the Kruppel-like zinc finger transcription factor ZBP-89 as a master regulator of early development of FLK1+ mesoderm into blood and blood vessels...
  11. Stem Cells For Myocardial Repair: A Large Bore Magnet Study
    Jianyi Zhang; Fiscal Year: 2012
    ..and differentiate at the single cell level into cells with phenotypic and functional characteristics of mesoderm, neuroectoderm, and endoderm lineages...
  12. Defining the role of the tumor-initiating cell niche in breast cancer
    Mei Zhang; Fiscal Year: 2011
    ..Insights into niche-TIC interactions may provide new therapeutic targets for drug development with the goal of both preventing breast cancer and reducing metastasis. ..
  13. Morphogenesis of mammalian gut endoderm
    Anna Katerina Hadjantonakis; Fiscal Year: 2012
    ..The prevailing view of germ layer formation in mammalian embryos is that ectoderm, mesoderm and gut endoderm derive solely from the epiblast during gastrulation and while extraembryonic tissues interact ..
  14. CONTROL OF MUSCLE PROTEIN SYNTHESIS DURING MYOGENESIS
    Charles P Emerson; Fiscal Year: 2013
    ..are the genetic and epigenetic mechanisms that control the earlier processes of skeletal myogenesis, including mesoderm specification and commitment of myogenic progenitors to the skeletal muscle lineage...
  15. Reprogramming of endothelial cells for improved cardiomyogenicity in vivo
    MELISSA ANN THAL; Fiscal Year: 2012
    ..Endothelial cells and cardiomyocytes both differentiate from a common progenitor in the mesoderm, suggesting that reprogramming endothelial cells back to that early state in mesoderm development could ..
  16. Epigenetic modification of endothelial progenitor cells into multipotent cells
    Rajasingh Johnson; Fiscal Year: 2010
    ..specific culture conditions, treated EPCs showed effective trans-differentiation into ectoderm (neuronal), and mesoderm (cardiomyocyte) lineages...
  17. Control of Cardiac Lineage Commitment and Differentiation in Murine Development
    Ibrahim J Domian; Fiscal Year: 2013
    ..this, we propose the following specific aims: 1) Determine if miR200 is promotes FHF development by supporting mesoderm differentiation and suppressing endoderm and ectoderm differentiation;2) Determine the mechanisms by which miR200 ..
  18. Genetic and Transcriptional Control of Spleen Development
    Licia Selleri; Fiscal Year: 2013
    ..We also found that Pbx1 expression commences earlier than that of both Nkx2.5 and Hox11 in the Lateral Plate Mesoderm (LPM). Additionally, we uncovered that Pbx1 is expressed in the endothelium of the developing spleen anlage...
  19. MOLECULAR CHARACTERIZATION OF THE DORSAL MORPHOGEN
    Michael S Levine; Fiscal Year: 2013
    ..For example, Snail, a key regulator of epithelial-mesenchyme transitions, is expressed in 800 mesoderm progenitor cells slated for invagination during gastrulation...
  20. Development of the renin-expressing cell
    Maria L Sequeira Lopez; Fiscal Year: 2010
    ..Although it has been accepted that renin cells derive from the mesoderm, our preliminary work indicates that either a subset or all of the renin-expressing cells may derive from ..
  21. Role of Fgfr2IIIb in epithelial mesenchymal signaling in intestinal development
    Peter F Nichol; Fiscal Year: 2013
    ..epithelial differentiation leading to alterations in growth, proliferation and Foxf1 gene expression in the mesoderm. Specific Aim 2: Test the hypothesis that loss of epithelial differentiation in the Fgfr2IIIb conditional mutants ..
  22. Uncovering the transcription factor networks in early human cell specification
    ALEXANDER MINCHEV TSANKOV; Fiscal Year: 2013
    ..During gastrulation, pluripotent epiblast cells give rise to the three germ layers-- endoderm, mesoderm, and ectoderm. Later in development, these layers form nearly all the different tissues and organs in our body...
  23. Role of Muscle Connective Tissue in Muscle and Tendon Development
    Gabrielle Kardon; Fiscal Year: 2013
    ..originating from the somites, while the muscle connective tissue, tendons, and skeletal elements derive from the mesoderm of the emerging lateral plate-derived limb bud...
  24. Role of FGF Signaling in Controlling Cell Movements During Drosophila Development
    ANGELIKE M STATHOPOULOS; Fiscal Year: 2013
    ..In Drosophila embryos, signaling through the Heartless FGFR is important for controlling mesoderm spreading during gastrulation and also, subsequently, for migration of caudal visceral mesoderm cells in the ..
  25. Deciphering T-box gene-dependent mesoderm development with synthetic probes
    James K Chen; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The transformation of vertebrate mesoderm into muscle, cartilage, bone, notochord, kidneys, gonads, blood, and other tissues is a classic example of morphogenesis and cellular differentiation during ..
  26. Defining the role of the tumor-initiating cell niche in breast cancer
    Mei Zhang; Fiscal Year: 2013
    ..Thus, I believe this will provide me an outstanding training environment that will facilitate my transition to become an independent scientist. ..
  27. NFkB Targets and Neural Crest Development
    MICHAEL WILLIAM KLYMKOWSKY; Fiscal Year: 2010
    ..embryo, Twist, Snail1, and Snail2 regulate one another's expression and are required for the formation of mesoderm and the subsequent formation, survival, and differentiation of the neural crest...
  28. Generation of hESC reporter lines using improved gene targeting technology
    TOBIAS DIETMAR RAABE; Fiscal Year: 2010
    ..a cardiac-specific transcription factor that is essential for heart development and, if enough time remains, the mesoderm marker T/Brachyury and cardiac specific myosin MYH6/alpha-MHC...
  29. Regulation of cardiovascular transcription
    Brian L Black; Fiscal Year: 2013
    ..that integrates upstream signals to direct expression in cardiovascular progenitors in the anterior lateral mesoderm. Two specific aims are proposed...
  30. Genetic Pathways Directing Ventral Folding Morphogenesis in Mammalian Embryos
    ELIZABETH H LACY; Fiscal Year: 2013
    ..to one or more distinct epiblast-derived cell types: gut endoderm, anterior neuroectoderm, and/or cardiac mesoderm. Upon receipt of the AVE-derived BMP2 signal, target tissues undergo morphogenetic changes in cell behaviors that ..
  31. Intracellular trafficking of Bisphosphonates in bone mesenchymal Stem Cells
    Sunday O Akintoye; Fiscal Year: 2013
    ..maxilla/mandible (OFMSCs) are highly proliferative with long population doublings relative to those of mesoderm-derived iliac bone (ICMSCs) and long bones (LBMSCs)...
  32. The role of Twist family bHLH factors in limb morphogenesis
    Anthony B Firulli; Fiscal Year: 2013
    ..class of Basic Helix-Loop-Helix (bHLH) proteins, which are expressed within the heart, branchial arches, lateral mesoderm and developing limb buds...
  33. Generation of Retinoid Signals during Development
    GREGG L DUESTER; Fiscal Year: 2013
    ..of retinol dehydrogenase (Rdh10) and retinaldehyde dehydrogenase (Raldh2) that together generate RA in trunk mesoderm just anterior to the caudal progenitor zone...
  34. Cellular and Molecular Mechanisms of Patterned Growth of the Mammalian Skull
    Robert E Maxson; Fiscal Year: 2013
    ..During skull vault development, cells derived from the neural crest and mesoderm migrate into positions above the eye, between the brain and a layer of non-osteogenic head mesoderm...
  35. Extrinsic signaling in neural crest and craniofacial development
    KIMBERLY ELAINE INMAN; Fiscal Year: 2010
    ..crest cell formation, migration and differentiation with extrinsic patterning cues derived from the surrounding mesoderm, ectoderm and endoderm...
  36. Allogeneic Placental Stem Cells for Tissue Repair and Regeneration
    Vijay Kumar; Fiscal Year: 2010
    ..mesenchymal stem cells (MSC), with the ability to differentiate to all three germ layers (ectoderm, endoderm, and mesoderm) in vivo...
  37. Regulation of call adhesion in Xenopus
    KEN CHO; Fiscal Year: 2009
    ..Growth factors known to be involved in mesoderm patterning such as Wnt and activin/nodal also influence mesodermal morphogenesis and cell shape...
  38. Regional differences in neural crest and mesodermal derived calvarial bone healin
    Michael T Longaker; Fiscal Year: 2013
    ..from the neural crest and the paired parietal bones, positioned posteriorly, are derived from the paraxial mesoderm. We have preliminarily observed the frontal bone to possess superior osteogenic potential both in vitro and in ..
  39. Myogenic stem cells in extraocular muscles
    Zipora Yablonka-Reuveni; Fiscal Year: 2013
    ..They develop from the head mesoderm and are molecularly, anatomically and physiologically distinct from other skeletal muscles...
  40. Transcriptional control of collective cell migration
    Lionel Christiaen; Fiscal Year: 2013
    ..of a chordate species, the ascidian Ciona intestinalis, will be used to study the migration of pre-cardiac mesoderm cells, called "trunk ventral cells" (TVCs)...
  41. Methods Development for CVM Cell Migration Study in Drosophila
    ANGELIKE M STATHOPOULOS; Fiscal Year: 2013
    ..To accomplish this goal, we must first develop methodology to facilitate study of caudal visceral mesoderm (CVM) cell migration in Drosophila...
  42. Role of FGF10 in liver organogenesis and alcohol-impaired regeneration
    KASPER SAONUN WANG; Fiscal Year: 2010
    ..FgflO, expressed in the embryonic foregut mesoderm, normally interacts with the adjacent endoderm through Fibroblast Growth Factor Receptor 2 1Mb (FGFR2b)...
  43. Modeling human trophoblast stem cells using iPS cells derived from molar placenta
    Mana M Parast; Fiscal Year: 2013
    ..trophoblast stem cells in this system, partly due to the mixture of other resulting lineages, in particular mesoderm, in early cultures following BMP4 addition...
  44. Mechanisms of anterior segment development and corneal neovascularization
    Tsutomu Kume; Fiscal Year: 2012
    ..The transcription factor Foxc1 is expressed in neural crest- and mesoderm-derived periocular mesenchyme surrounding the developing eye...
  45. Roles of hedgehog signaling in foregut development
    JOHN A KLINGENSMITH; Fiscal Year: 2013
    ..and esophagus is critical for the stratification of foregut epithelia and development of specialized foregut mesoderm. Our overall hypothesis is that shh signaling acts as a master regulator of foregut development, initially to ..
  46. Genetic Regulation of Progenitor Cells in Appendicular Skeletal Development
    Yasuhiko Kawakami; Fiscal Year: 2013
    ..the body extension, progressively generates progenitor cells for various tissues, including the lateral plate mesoderm (LPM)...
  47. Function of epithelial-mesenchymal transition during pulmonary fibrosis
    KEVIN KEEWOUN KIM; Fiscal Year: 2013
    ..These studies will help define previously unexplored mechanisms that may regulate fibrosis with the potential of designing new and better therapies. ..
  48. Ectoderm formation in the early Xenopus embryo
    Matthew Kofron; Fiscal Year: 2013
    ..by Principal Investigator): All tissues of the vertebrate body arise from three primary germ layers;the ectoderm, mesoderm and endoderm. The ectoderm gives rise to the central nervous system and epidermis...
  49. Rapid Multiplexed Nanoprobe Assays for Pluripotent Stem Cell Differentiation
    David Larocca; Fiscal Year: 2013
    ..develop a multiplexed PTQD assay for definitive endoderm (DE) and identify additional peptides for ectoderm and mesoderm assays...
  50. Transcriptional regulation of liver specification in Xenopus tropicalis
    ANDREA ELIZABETH WILLS; Fiscal Year: 2012
    ..of extracellular signals including BMP signals from the septum transversum and FGF signals from the cardiac mesoderm. The goal of this research is to answer three unresolved questions in liver fate specification: which of the ..
  51. Epidemiology of Syndromic GI Stromal Tumors
    Judy Ellen Garber; Fiscal Year: 2010
    ..Our goal is to define the spectrum of syndromic GISTs, and to generate the information that will form the basis for clinical counseling for members of GIST kindreds, with and without germline mutations in KIT or PDGFRA. ..
  52. DORSAL-VENTRAL PATTERN FORMATION IN THE ZEBRAFISH EMBRYO
    Mary C Mullins; Fiscal Year: 2013
    ..the past grant period, which when deficient leads to the ventral expansion of BMP antagonists and dorsal midline mesoderm causing the dramatic formation of multiple dorsal axes...
  53. Genomic Analysis of Cis Regulatory Networks of Drosophila Muscle Development
    Leila Shokri; Fiscal Year: 2011
    ..driving gene expression during the specification of somatic muscle founder cell fate in the developing Drosophila mesoderm. To evaluate the potential PBM-derived and predicted cis regulatory codes, the effects of RNAi knockdown on the ..
  54. Skeletal Muscle Regeneration from Differentiating Embryonic Stem Cells
    Rita C R Perlingeiro; Fiscal Year: 2012
    ..not been used to investigate the molecular mechanisms of skeletal muscle lineage determination acting in nascent mesoderm. Similarly, in spite of the interest in and hype surrounding the potential use of ES cells to treat diseases of ..
  55. Hypospadias: Morphogenesis of the Proximal Urethra
    Christine E Larkins; Fiscal Year: 2013
    ..The urethra is formed from a plate of endodermally derived epithelium that is surrounded by the mesoderm of the developing external genitalia, or genital tubercle (GT)...
  56. Engineering Stem Cell Niches to Guide Differentiation
    DANIELLE A TSOU; Fiscal Year: 2011
    ..The hematopoietic differentiation pathways in the murine system have been established, and a specific ESC line will allow simultaneous monitoring of mesoderm and hematopoietic differentiation.
  57. Murine Models for Regenerative Medicine
    George Daley; Fiscal Year: 2009
    This proposal will explore genetic pathways that specify the commitment of embryonic mesoderm toward hematopoietic fate in model organisms like the fish and mouse, in order to identify mechanisms that can be engineered to drive ..
  58. Regionalization of the Vertebrate Endoderm
    Victoria E Prince; Fiscal Year: 2013
    ..period, we used this approach to establish that the signaling molecule Retinoic Acid (RA) is an instructive mesoderm-derived positive regulator of pancreas development, which acts directly on endoderm to specify the foregut region...
  59. Does Arsenic Target Border Specific Cells during Embryogenesis?
    Lisa J Bain; Fiscal Year: 2013
    ..repressive signaling pathway targeted by arsenic during the formation of the neural plate, neural crest, and the mesoderm. In the first aim, we will examine changes in the localization and number of the neural plate border specifier ..
  60. Vascular Differentiation and Patterning
    Takashi Mikawa; Fiscal Year: 2012
    ..notochord and BMP antagonists can suppress the expression of endothelial cell differentiation markers in cultured mesoderm;b) BMP antagonist-treatment of differentiated endothelial cells results in a rapid loss of VEGF receptor Vegfr2 ..
  61. BIOLOGICAL ROLES OF NODAL RELATED GENES IN EMBRYOGENESIS
    Christopher V Wright; Fiscal Year: 2010
    ..of how the Xnr1 expression pattern is transformed into a "response-activity map" within L lateral plate mesoderm (LPM) at the level of intranuclear phospho-Smad2 (P-Smad2), and how this map correlates with the cell and tissue ..
  62. Epigenetic mechanisms and genes that regulate adipose tissue expansion
    Robert A Koza; Fiscal Year: 2013
    ..One of these genes, mesoderm specific transcript (Mest);a maternally imprinted gene localized in the endoplasmic reticulum/Golgi apparatus ..
  63. Role of the cdx homeobox genes in the regulation of wt1 and nephron development
    Alan J Davidson; Fiscal Year: 2010
    ..The zebrafish embryo is ideal for these studies as the intermediate mesoderm on either side of the trunk forms two simple linear nephrons...
  64. CELL MOVEMENT DURING XENOPUS DEVELOPMENT--METASTASIS
    Karen Symes; Fiscal Year: 2004
    ..We have shown that in Xenopus embryos inhibition of PDGF signaling prevents mesoderm cell movement (1)...
  65. FIBROBLAST GROWTH FACTOR RECEPTOR IN EARLY DEVELOPMENT
    Thomas Musci; Fiscal Year: 1992
    ..Basic fibroblast growth factor (bFGF) is present in the early embryo and will induce mesoderm formation in vitro. Direct evidence for the requirement of FGF to form mesoderm in vivo has been lacking...
  66. Mechanisms of Early Kidney Development
    TERI MAUCH; Fiscal Year: 2007
    ..The pronephros arises directly from intermediate mesoderm (IM) and represents the first stage of vertebrate kidney induction...
  67. Development of Avian Extraocular Muscles and Nerves
    Drew Noden; Fiscal Year: 2007
    ..g., fgf8) act upon adjacent paraxial mesoderm cells...
  68. Global Patterning in Vertebrate Mesoderm
    Ann Burke; Fiscal Year: 2005
    ..The cells that form all of the key parts of the musculoskeletal system arise from two populations of embryonic mesoderm, the somites and somatic lateral plate (abbreviated here as LP)...
  69. Delta and Mesoderm Induction in Eucidaris Embryos
    Hyla Sweet; Fiscal Year: 2004
    ..The long-term goal of this research is to examine the evolution of mesoderm development within the echinoderm phylum...