protozoan genome

Summary

Summary: The complete genetic complement contained in a set of CHROMOSOMES in a protozoan (PROTOZOA).

Top Publications

  1. ncbi Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena
    Kazufumi Mochizuki
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Cell 110:689-99. 2002
  2. ncbi Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
  3. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
  4. pmc Genome sequence of the human malaria parasite Plasmodium falciparum
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:498-511. 2002
  5. ncbi Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum
    John C Wootton
    Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894 6075, USA
    Nature 418:320-3. 2002
  6. pmc GiardiaDB and TrichDB: integrated genomic resources for the eukaryotic protist pathogens Giardia lamblia and Trichomonas vaginalis
    Cristina Aurrecoechea
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 37:D526-30. 2009
  7. ncbi Genome of the host-cell transforming parasite Theileria annulata compared with T. parva
    Arnab Pain
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 309:131-3. 2005
  8. pmc The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum
    Zbynek Bozdech
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 1:E5. 2003
  9. pmc Genome-wide gene expression profiling analysis of Leishmania major and Leishmania infantum developmental stages reveals substantial differences between the two species
    Annie Rochette
    Research Centre in Infectious Diseases, CHUL Research Centre and Department of Medical Biology, Faculty of Medicine, Laval University, Quebec, Canada
    BMC Genomics 9:255. 2008
  10. pmc Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis
    Jane M Carlton
    Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
    Science 315:207-12. 2007

Research Grants

  1. Genome Evolution, Innovation and Adaptation in the Apicomplexa
    Jessica C Kissinger; Fiscal Year: 2010
  2. Novel Anti-Malarials by Combinatorial Pharmacogenomics
    Joseph DeRisi; Fiscal Year: 2006
  3. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
  4. GENE DISCOVERY IN TOXOPLASMA GONDII
    L Sibley; Fiscal Year: 2003
  5. Microfilaments in Giardia Attachment and Virulence
    HEIDI ELMENDORF; Fiscal Year: 2006
  6. Calcium ATPases in Apicomplexan Parasites as Potential D
    L Sibley; Fiscal Year: 2006
  7. Mucosal Immunity to Toxoplasma gondii
    L Sibley; Fiscal Year: 2007
  8. Confocal Microscope for Microbiology
    L Sibley; Fiscal Year: 2002
  9. Transcriptional Regulation in Giardia lamblia
    HEIDI ELMENDORF; Fiscal Year: 2006
  10. GENETIC DISSECTION OF TOXOPLASMA GONDII
    David Roos; Fiscal Year: 2008

Detail Information

Publications216 found, 100 shown here

  1. ncbi Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena
    Kazufumi Mochizuki
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Cell 110:689-99. 2002
    ..We propose that these small RNAs function to specify sequences to be eliminated by a mechanism similar to RNA-mediated gene silencing...
  2. ncbi Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
    ..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
  3. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  4. pmc Genome sequence of the human malaria parasite Plasmodium falciparum
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:498-511. 2002
    ..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
  5. ncbi Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum
    John C Wootton
    Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894 6075, USA
    Nature 418:320-3. 2002
    ..The presence of linkage disequilibrium provides a basis for mapping genes under drug selection in P. falciparum...
  6. pmc GiardiaDB and TrichDB: integrated genomic resources for the eukaryotic protist pathogens Giardia lamblia and Trichomonas vaginalis
    Cristina Aurrecoechea
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 37:D526-30. 2009
    ..Phylogenetic relationships may also be interrogated. The ability to combine the results from independent queries, and to store queries and query results for future use facilitates complex, genome-wide mining of functional genomic data...
  7. ncbi Genome of the host-cell transforming parasite Theileria annulata compared with T. parva
    Arnab Pain
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 309:131-3. 2005
    ....
  8. pmc The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum
    Zbynek Bozdech
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 1:E5. 2003
    ..falciparum and provide a resource for the identification of new chemotherapeutic and vaccine candidates...
  9. pmc Genome-wide gene expression profiling analysis of Leishmania major and Leishmania infantum developmental stages reveals substantial differences between the two species
    Annie Rochette
    Research Centre in Infectious Diseases, CHUL Research Centre and Department of Medical Biology, Faculty of Medicine, Laval University, Quebec, Canada
    BMC Genomics 9:255. 2008
    ....
  10. pmc Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis
    Jane M Carlton
    Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
    Science 315:207-12. 2007
    ..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
  11. ncbi The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease
    Najib M El-Sayed
    Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
    Science 309:409-15. 2005
    ....
  12. pmc Comparative genomics of the neglected human malaria parasite Plasmodium vivax
    Jane M Carlton
    The Institute for Genomic Research J Craig Venter Institute, 9704 Medical Research Drive, Rockville, Maryland 20850, USA
    Nature 455:757-63. 2008
    ..Completion of the P. vivax genome provides the scientific community with a valuable resource that can be used to advance investigation into this neglected species...
  13. pmc dictyBase: a new Dictyostelium discoideum genome database
    Lisa Kreppel
    Laboratory of Cellular and Developmental Biology 50 3351, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Nucleic Acids Res 32:D332-3. 2004
    ....
  14. ncbi Genomic minimalism in the early diverging intestinal parasite Giardia lamblia
    Hilary G Morrison
    Marine Biological Laboratory, Woods Hole, MA 02543 1015, USA
    Science 317:1921-6. 2007
    ..This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite...
  15. ncbi A three-genome phylogeny of malaria parasites (Plasmodium and closely related genera): evolution of life-history traits and host switches
    Ellen S Martinsen
    Department of Biology, University of Vermont, Burlington, VT 05405, USA
    Mol Phylogenet Evol 47:261-73. 2008
    ..The Plasmodium of birds and squamate reptiles all fall within a single clade, with evidence for repeated switching between birds and squamate hosts...
  16. ncbi The genome of the protist parasite Entamoeba histolytica
    Brendan Loftus
    TIGR, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 433:865-8. 2005
    ..Analysis of the genome provides new insights into the workings and genome evolution of a major human pathogen...
  17. pmc ToxoDB: an integrated Toxoplasma gondii database resource
    Bindu Gajria
    Department of Biology, Department of Genetics, Center for Bioinformatics, University of Pennsylvania, Philadelphia, PA, USA
    Nucleic Acids Res 36:D553-6. 2008
    ..ToxoDB has matured significantly since its initial release. Here we outline the numerous updates with respect to the data and increased functionality available on the website...
  18. ncbi Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia
    Jean Marc Aury
    Genoscope and CNRS UMR 8030, 2 rue Gaston Crémieux CP5706, 91057 Evry, France
    Nature 444:171-8. 2006
    ..The conclusion of this analysis is that many genes are maintained after whole-genome duplication not because of functional innovation but because of gene dosage constraints...
  19. ncbi Selective upregulation of a single distinctly structured var gene in chondroitin sulphate A-adhering Plasmodium falciparum involved in pregnancy-associated malaria
    Ali Salanti
    Centre for Medical Parasitology at the Institute for Medical Microbiology and Immunology, University of Copenhagen, Panum Institute 24 2, and Department of Infectious Diseases, Copenhagen University Hospital Rigshospitalet, Denmark
    Mol Microbiol 49:179-91. 2003
    ..falciparum malaria. This var gene thus has the properties expected of a gene encoding the parasite adhesion molecule that initiates the pathology associated with PAM...
  20. pmc Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii
    Asis Khan
    Department of Molecular Microbiology, Center for Infectious Diseases, Washington University School of Medicine St Louis, MO 63110, USA
    Nucleic Acids Res 33:2980-92. 2005
    ..The resulting genome map provides a framework for analysis of complex traits such as virulence and transmission, and for comparative population genetic studies...
  21. pmc Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote
    Jonathan A Eisen
    The Institute for Genomic Research, Rockville, Maryland, United States of America
    PLoS Biol 4:e286. 2006
    ..thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance...
  22. pmc Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
    T Nicolai Siegel
    Laboratory of Molecular Parasitology, The Rockefeller University, New York, New York 10065, USA
    Genes Dev 23:1063-76. 2009
    ....
  23. pmc Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila research
    Nicholas A Stover
    Department of Genetics, School of Medicine, Stanford University, Stanford, CA 94305 5120, USA
    Nucleic Acids Res 34:D500-3. 2006
    ..A variety of tools are available at TGD for searching the Tetrahymena genome, its literature and information about members of the research community...
  24. pmc Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophila
    Colin D Malone
    Biology Department, Washington University, St Louis, MO 63130, USA
    Mol Cell Biol 25:9151-64. 2005
    ....
  25. ncbi The Trypanosoma cruzi proteome
    J A Atwood
    Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
    Science 309:473-6. 2005
    ..The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes...
  26. pmc Comparative analysis of the kinomes of three pathogenic trypanosomatids: Leishmania major, Trypanosoma brucei and Trypanosoma cruzi
    Marilyn Parsons
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    BMC Genomics 6:127. 2005
    ..As a part of an effort to understand parasite signaling functions, we report the results of a genome-wide analysis of protein kinases (PKs) of these three trypanosomatids...
  27. ncbi Evidence for multiple hybrid groups in Trypanosoma cruzi
    Nancy R Sturm
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, 609 Charles E Young Drive East, Los Angeles, CA 90095 1489, USA
    Int J Parasitol 33:269-79. 2003
    ..These data demonstrate for the first time that IIa/IIc are hybrid, consistent with the hypothesis that genetic recombination has occurred more than once within the T. cruzi lines...
  28. pmc A functional role for transposases in a large eukaryotic genome
    Mariusz Nowacki
    Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
    Science 324:935-8. 2009
    ..This study suggests a new important role in Oxytricha for this large portion of genomic DNA that was previously thought of as junk...
  29. pmc The genetic map and comparative analysis with the physical map of Trypanosoma brucei
    Annette MacLeod
    Wellcome Centre for Molecular Parasitology, Anderson College Complex, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Nucleic Acids Res 33:6688-93. 2005
    ....
  30. pmc Cryptosporidium parvum genes containing thrombospondin type 1 domains
    Mingqi Deng
    Department of Veterinary Pathobiology, University of Minnesota, St Paul 55108, USA
    Infect Immun 70:6987-95. 2002
    ..Only CpTSP4 was highly expressed solely at an early stage of infection...
  31. ncbi Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum
    Annie Rochette
    Research Centre in Infectious Disease, CHUL Research Centre, Quebec, Canada G1V 4G2
    Mol Biochem Parasitol 165:32-47. 2009
    ..These findings highlight the importance of the host macrophage in driving the parasite to specific adaptations, which consequently result in highly regulated changes in gene expression...
  32. pmc Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Proc Natl Acad Sci U S A 100:9952-7. 2003
    ..The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens...
  33. ncbi Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi
    M D Katinka
    Genoscope, UMR CNRS 8030, CP 5706, 91057 Evry Cedex, France
    Nature 414:450-3. 2001
    ..Because the E. cuniculi genome contains genes related to some mitochondrial functions (for example, Fe-S cluster assembly), we hypothesize that microsporidia have retained a mitochondrion-derived organelle...
  34. pmc The steady-state transcriptome of the four major life-cycle stages of Trypanosoma cruzi
    Todd A Minning
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
    BMC Genomics 10:370. 2009
    ..cruzi and related kinetoplastid parasites have suggested that the transcript abundances of most genes in these organisms do not vary significantly between life-cycle stages...
  35. ncbi From genome to vaccines for leishmaniasis: screening 100 novel vaccine candidates against murine Leishmania major infection
    Carmel B Stober
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, UK
    Vaccine 24:2602-16. 2006
    ..Two ribosomal proteins, a V-ATPase subunit, and a dynein light chain orthologue were the only other protective genes with putative functions...
  36. pmc The genome of the simian and human malaria parasite Plasmodium knowlesi
    A Pain
    Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Nature 455:799-803. 2008
    ..One of these families, the KIRs, contains sequences that collectively match over one-half of the host CD99 extracellular domain, which may represent an unusual form of molecular mimicry...
  37. pmc GOtcha: a new method for prediction of protein function assessed by the annotation of seven genomes
    David M A Martin
    Post Genomics and Molecular Interactions Centre, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    BMC Bioinformatics 5:178. 2004
    ..GOtcha predicts GO term associations with term-specific probability (P-score) measures of confidence. Term-specific probabilities are a novel feature of GOtcha and allow the identification of conflicts or uncertainty in annotation...
  38. ncbi Two families of rep-like genes that probably originated by interspecies recombination are represented in viral, plasmid, bacterial, and parasitic protozoan genomes
    Mark J Gibbs
    Mol Biol Evol 23:1097-100. 2006
    ....
  39. pmc Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruzi
    Bruno Dallagiovanna
    Instituto de Biologia Molecular do Paraná, Rua Professor Algacyr Munhoz Mader 3775, Curitiba, PR, Brazil
    J Biol Chem 283:8266-73. 2008
    ..Our results suggest that TcPUF6 regulates the half-lives of its associated transcripts via differential association with mRNA degradation complexes throughout its life cycle...
  40. pmc JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomes
    Laura J Cliffe
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    Nucleic Acids Res 37:1452-62. 2009
    ..Reintroduction of JBP2 in the J-null trypanosome stimulates HOMedU formation and site-specific synthesis of base J. We conclude that JBP2 and JBP1 are the TH enzymes involved in J biosynthesis...
  41. ncbi A phylogenomic inventory of meiotic genes; evidence for sex in Giardia and an early eukaryotic origin of meiosis
    Marilee A Ramesh
    Department of Biology, Roanoke College, Salem, VA 24153, USA
    Curr Biol 15:185-91. 2005
    ....
  42. ncbi Host switch leads to emergence of Plasmodium vivax malaria in humans
    Jianbing Mu
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA
    Mol Biol Evol 22:1686-93. 2005
    ..Taken together, a haplotype network, parasite migration patterns, demographic history, and cophylogeny mapping support an Asian origin via a host switch from macaque monkeys...
  43. ncbi The LINEs and SINEs of Entamoeba histolytica: comparative analysis and genomic distribution
    Abhijeet A Bakre
    School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Exp Parasitol 110:207-13. 2005
    ..The elements were very frequently located close to protein-coding genes and other EhLINEs/SINEs. The possible influence of these elements on expression of neighboring genes needs to be determined...
  44. ncbi Reassessment of the epidemiology of amebiasis: state of the art
    Cecilia Ximenez
    Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico DF, Mexico
    Infect Genet Evol 9:1023-32. 2009
    ..Finally, we will discuss those areas of the host-parasite relationship that are still not fully understood, and the scientific challenges to approach this important public health problem in the future...
  45. pmc The genome of the kinetoplastid parasite, Leishmania major
    Alasdair C Ivens
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Science 309:436-42. 2005
    ..Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression...
  46. ncbi Detecting selection using a single genome sequence of M. tuberculosis and P. falciparum
    Joshua B Plotkin
    Harvard Society of Fellows and Bauer Center for Genomics Research, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nature 428:942-5. 2004
    ..Our method of estimating selective pressures requires far fewer data than comparative sequence analysis, and it measures selection across an entire genome; the method can readily be applied to a large range of sequenced organisms...
  47. pmc Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites
    Jake Baum
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
    Nucleic Acids Res 37:3788-98. 2009
    ....
  48. ncbi Leishmania: conserved evolution--diverse diseases
    Miriam A Lynn
    Immunity and Infection Research Centre, Vancouver Coastal Health Institute, and the Department of Medical Genetics, University of British Columbia, 2660 Oak Street, Vancouver, V6H 3Z6 BC, Canada
    Trends Parasitol 24:103-5. 2008
    ..Here, we discuss these findings together with recent microarray and proteomics studies and highlight their importance in understanding Leishmania disease phenotypes...
  49. pmc Genome-wide characterization of tetrahymena thermophila chromosome breakage sites. I. Cloning and identification of functional sites
    Eileen Hamilton
    Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, California 93106, USA
    Genetics 170:1611-21. 2005
    ....
  50. ncbi Large-scale genome remodelling by the developmentally programmed elimination of germ line sequences in the ciliate Paramecium
    Mireille Bétermier
    CNRS UMR 8541, Laboratoire de Genetique Moleculaire, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
    Res Microbiol 155:399-408. 2004
    ..Recent data point to a role of non-coding RNAs in the epigenetic programming of these rearrangements...
  51. pmc Kinetoplastids: related protozoan pathogens, different diseases
    Ken Stuart
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    J Clin Invest 118:1301-10. 2008
    ....
  52. ncbi Initiator and upstream elements in the alpha2-tubulin promoter of Giardia lamblia
    H G Elmendorf
    Laboratory of Parasitic Diseases, NIH, Bethesda, MD 20892 0425, USA
    Mol Biochem Parasitol 113:157-69. 2001
    ..In particular, the short and diverse sequences that function in transcription initiation in Giardia suggest the potential for relaxed transcriptional regulation...
  53. pmc Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens
    Karina Picazarri
    Department of Parasitology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Infect Immun 76:278-88. 2008
    ..invadens. These results suggest that the core machinery for autophagy is conserved and plays an important role during proliferation and differentiation in Entamoeba...
  54. ncbi Sequence of Plasmodium falciparum chromosome 12
    Richard W Hyman
    Stanford Genome Technology Center, 855 California Avenue, Palo Alto, California 94304, USA
    Nature 419:534-7. 2002
    ..We discuss the methodology that yielded a finished and fully contiguous sequence for chromosome 12. The biological implications of the sequence data are more thoroughly discussed in an accompanying Article (ref. 3)...
  55. pmc Evolution of tubulin gene arrays in Trypanosomatid parasites: genomic restructuring in Leishmania
    Andrew P Jackson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    BMC Genomics 7:261. 2006
    ..brucei. Polycistronic transcription in these organisms makes the chromosomal arrangement of tubulin genes important with respect to gene expression...
  56. ncbi Functional genomics in Dictyostelium: MidA, a new conserved protein, is required for mitochondrial function and development
    Patricia Torija
    Instituto de Investigaciones Biomédicas Alberto Sols C S I C U A M, Calle Arturo Duperier 4, 28029 Madrid, Spain
    J Cell Sci 119:1154-64. 2006
    ..This work exemplifies the potential of Dictyostelium as a model system for functional genomic studies...
  57. pmc Molecular characterization of serine-, alanine-, and proline-rich proteins of Trypanosoma cruzi and their possible role in host cell infection
    Renata C P Baida
    Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862, CEP 04023 062, Sao Paulo, Brazil
    Infect Immun 74:1537-46. 2006
    ....
  58. ncbi Rapid turnover of Plasmodium falciparum var gene transcripts and genotypes during natural non-symptomatic infections
    Gerhard Wunderlich
    Departamento de Parasitologia, Instituto de Ciências Biomédicas 2, Universidade de Sao Paulo, 05508 900 Sao Paulo, SP, Brazil
    Rev Inst Med Trop Sao Paulo 47:195-201. 2005
    ....
  59. ncbi Gene synteny and evolution of genome architecture in trypanosomatids
    Elodie Ghedin
    Parasity Genomics, The Institute for Genomics Research, 9712 Medical Center Dr Rockville, MD 20850, USA
    Mol Biochem Parasitol 134:183-91. 2004
    ..major by examining the regions near breakage points of the synteny. This is the first such element found in L. major suggesting that retroelements were found in the common ancestor of all three species...
  60. pmc A spliceosomal intron in Giardia lamblia
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115 6018, USA
    Proc Natl Acad Sci U S A 99:3701-5. 2002
    ..If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia...
  61. pmc A novel family of mobile genetic elements is limited to the germline genome in Tetrahymena thermophila
    Jeffrey D Wuitschick
    Department of Biology, Marquette University, Milwaukee, WI 53201 1881, USA
    Nucleic Acids Res 30:2524-37. 2002
    ..Possible mechanisms of developmentally programmed Tlr elimination are discussed...
  62. ncbi Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes
    Malcolm J Gardner
    Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:134-7. 2005
    ..Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated...
  63. ncbi Structure and content of the Entamoeba histolytica genome
    C G Clark
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Adv Parasitol 65:51-190. 2007
    ..In this chapter we summarise the features of the genome as they are currently understood and provide previously unpublished analyses of many of the genes...
  64. pmc Integrated mapping, chromosomal sequencing and sequence analysis of Cryptosporidium parvum
    Alan T Bankier
    Medical Research Council MRC Laboratory of Molecular Biology, Cambridge CB 2 2QH, UK
    Genome Res 13:1787-99. 2003
    ..parvum, whereas the other is shared with (but has previously gone unnoticed in) all known genomes of the Coccidia and Haemosporida. These motifs appear to be unique in their structure, distribution and sequences...
  65. pmc TriTrypDB: a functional genomic resource for the Trypanosomatidae
    Martin Aslett
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Nucleic Acids Res 38:D457-62. 2010
    ....
  66. ncbi The Eimeria genome projects: a sequence of events
    Martin W Shirley
    Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berkshire RG20 7NN, UK
    Trends Parasitol 20:199-201. 2004
  67. pmc Population genomics of the immune evasion (var) genes of Plasmodium falciparum
    Alyssa E Barry
    Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, United Kingdom
    PLoS Pathog 3:e34. 2007
    ..This population genomic framework provides a cost effective molecular epidemiological tool to rapidly explore the geographic diversity of var genes...
  68. pmc Ancestral genomes, sex, and the population structure of Trypanosoma cruzi
    Jorge M de Freitas
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    PLoS Pathog 2:e24. 2006
    ..cruzi II and T. cruzi III produced evolutionarily viable progeny. In both events, the mitochondrial recipient (as identified by the mitochondrial clade of the hybrid strains) was T. cruzi II and the mitochondrial donor was T. cruzi III...
  69. ncbi Evidence for the importance of genetic structuring to the structural and functional specialization of the Plasmodium falciparum var gene family
    Susan M Kraemer
    Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109, USA
    Mol Microbiol 50:1527-38. 2003
    ....
  70. ncbi The genome of Entamoeba histolytica
    A Bhattacharya
    School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi, India
    Int J Parasitol 30:401-10. 2000
    ..The Expressed Sequence Tag approach has also helped identify several important genes which encode proteins that may be involved in different biochemical pathways, signal transduction mechanisms and organellar functions...
  71. ncbi Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14
    Malcolm J Gardner
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:531-4. 2002
    ..We report here the nucleotide sequences of chromosomes 10, 11 and 14, and a re-analysis of the chromosome 2 sequence. These chromosomes represent about 35% of the 23-megabase P. falciparum genome...
  72. ncbi Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13
    N Hall
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 419:527-31. 2002
    ..We identify a highly conserved sequence element found in the intergenic region of internal var genes that is not associated with their telomeric counterparts...
  73. ncbi The Plasmodium vivax genome sequencing project
    Jane Carlton
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Trends Parasitol 19:227-31. 2003
    ..Here, an update on the Plasmodium vivax genome sequencing project is presented, as part of the Trends in Parasitology series of reviews expanding on various aspects of P. vivax research...
  74. pmc Utility of the Trypanosoma cruzi sequence database for identification of potential vaccine candidates by in silico and in vitro screening
    Vandanajay Bhatia
    Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston 77555, USA
    Infect Immun 72:6245-54. 2004
    ..cruzi. Taken together, our results validate the applicability of bioinformatics in genome mining, resulting in the identification of T. cruzi membrane-associated proteins that are potential vaccine candidates...
  75. ncbi Robust method for proteome analysis by MS/MS using an entire translated genome: demonstration on the ciliome of Tetrahymena thermophila
    Jeffrey C Smith
    Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
    J Proteome Res 4:909-19. 2005
    ..This methodology allows identifying the locations of the genes encoding these novel proteins, which is a necessary first step to downstream functional genomic experimentation...
  76. ncbi Trichomonas vaginalis surface proteins: a view from the genome
    R P Hirt
    Institute for Cellular and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
    Trends Parasitol 23:540-7. 2007
    ..Here, we review the existing data on T. vaginalis surface proteins and summarize some of the main findings from the recent in silico characterization of its candidate surface proteins...
  77. ncbi Complete genome sequence of the apicomplexan, Cryptosporidium parvum
    Mitchell S Abrahamsen
    Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
    Science 304:441-5. 2004
    ..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
  78. pmc tRNA gene sequences are required for transcriptional silencing in Entamoeba histolytica
    Henriette Irmer
    Grisebachstr 8, Institute of Microbiology and Genetics, Department of Molecular Microbiology and Genetics, Georg August University, Gottingen, 37077 Gottingen, Germany
    Eukaryot Cell 9:306-14. 2010
    ..histolytica promoter, opening a way toward future silencing of individual genes of interest in E. histolytica. Our results indicate that tRNA gene-mediated silencing is not restricted to Saccharomyces cerevisiae...
  79. pmc A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes
    Shane R Wilkinson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, United Kingdom
    Proc Natl Acad Sci U S A 105:5022-7. 2008
    ..This potential for drug resistance by a simple mechanism has important implications, because nifurtimox is currently undergoing phase III clinical trials against African trypanosomiasis...
  80. pmc An expanded inventory of conserved meiotic genes provides evidence for sex in Trichomonas vaginalis
    Shehre Banoo Malik
    Department of Biology, Roy J Carver Center for Comparative Genomics, University of Iowa, Iowa City, Iowa, United States of America
    PLoS ONE 3:e2879. 2008
    ....
  81. pmc ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic data
    Olivier Arnaiz
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Nucleic Acids Res 35:D439-44. 2007
    ..Future ParameciumDB development plans include coordination of human curation of the near 40 000 gene models by members of the research community...
  82. ncbi Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp
    Russell F Watkins
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5
    Protist 159:269-81. 2008
    ....
  83. ncbi Annexin-like alpha giardins: a new cytoskeletal gene family in Giardia lamblia
    Malin E L Weiland
    Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
    Int J Parasitol 35:617-26. 2005
    ..These localisation experiments suggest alpha-giardins play a role in cell motility, attachment and membrane stability...
  84. ncbi A proteomic view of the Plasmodium falciparum life cycle
    Laurence Florens
    Department of Cell Biology, The Scripps Research Institute, SR 11, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 419:520-6. 2002
    ..The detection of chromosomal clusters encoding co-expressed proteins suggested a potential mechanism for controlling gene expression...
  85. pmc Hemizygous subtelomeres of an African trypanosome chromosome may account for over 75% of chromosome length
    Sergio Callejas
    Department of Pathology, University of Cambridge, CB2 1QP, Cambridge, United Kingdom
    Genome Res 16:1109-18. 2006
    ..Further experimentation is required to determine if these results are applicable to all size-variable chromosomes...
  86. ncbi The Giardia lamblia genome
    R D Adam
    Department of Microbiology Immunology and Medicine, University of Arizona College of Medicine, 1501 N Campbell, Tucson 85724, USA
    Int J Parasitol 30:475-84. 2000
    ..The results will facilitate a better understanding of the biology of Giardia spp. as well as their phylogenetic relationship to other primitive organisms...
  87. ncbi Cryptosporidium parvum appears to lack a plastid genome
    G Zhu
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 2002, USA
    Microbiology 146:315-21. 2000
    ..It also suggests that unlike the situation with other diseases caused by members of the Apicomplexa, drug development against cryptosporidiosis targeting a plastid genome or metabolic pathways associated with it may not be useful...
  88. pmc Gene encoding erythrocyte binding ligand linked to blood stage multiplication rate phenotype in Plasmodium yoelii yoelii
    Sittiporn Pattaradilokrat
    Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, United Kingdom
    Proc Natl Acad Sci U S A 106:7161-6. 2009
    ..Our genome-wide LGS analysis also indicated effects of at least 1 other locus on multiplication rate, as did the findings of Otsuki and colleagues on virulence in P. y. yoelii...
  89. ncbi A novel palmitoyl acyl transferase controls surface protein palmitoylation and cytotoxicity in Giardia lamblia
    Maria C Touz
    Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA
    Mol Microbiol 58:999-1011. 2005
    ..These results show that VSPs are palmitoylated at the cysteine within the conserved tail by gPAT and indicate an essential function of palmitoylation in control of VSP-mediated signalling and processing...
  90. ncbi Numerous, robust genetic markers for Plasmodium chabaudi by the method of amplified fragment length polymorphism
    Katrina Grech
    University of Edinburgh, Division of Biological Sciences, ICAPB, Ashworth Laboratory, West Mains Road, Edinburgh EH9 3JT, UK
    Mol Biochem Parasitol 123:95-104. 2002
    ..6 x 10(-4) per base pair. This is consistent with the sequence diversity found between alleles of candidate drug resistance genes from P. c. chabaudi clones AS and AJ identified and sequenced in this laboratory...
  91. ncbi Fragmentation of mitochondrial large subunit rRNA in the dinoflagellate Alexandrium catenella and the evolution of rRNA structure in alveolate mitochondria
    Ryoma Kamikawa
    Laboratory of Marine Microbiology, Division of Applied Biosciences, Department of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Protist 158:239-45. 2007
    ..However, current sequence data on dinoflagellate mitochondria are insufficient to reject the alternative scenario, in which the rRNA fragmentation evolved independently in apicomplexan and dinoflagellate mitochondria...
  92. ncbi Malaria: new vaccines for old?
    Andrew Waters
    Department of Parasitology, Leiden University Medical Centre, 2333ZA Leiden, The Netherlands
    Cell 124:689-93. 2006
    ..Detailed analyses of the 5500 genes revealed by the complete Plasmodium genome sequence are yielding new candidate parasite antigens and strategies that may contribute to a successful vaccine against malaria in the coming decade...
  93. ncbi Differential expression of Leishmania major beta-tubulin genes during the acquisition of promastigote infectivity
    R M Coulson
    Department of Pathology, University of Cambridge, UK
    Mol Biochem Parasitol 82:227-36. 1996
    ..These data demonstrate that the regulation of mRNA half-life contributes to controlling gene expression as promastigotes differentiate into an infectious form...
  94. pmc Inconsistencies of genome annotations in apicomplexan parasites revealed by 5'-end-one-pass and full-length sequences of oligo-capped cDNAs
    Hiroyuki Wakaguri
    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan
    BMC Genomics 10:312. 2009
    ..To improve upon the current genome annotations, as well as to validate the importance for physical cDNA clone resources, we generated a large-scale collection of full-length cDNAs for several apicomplexa parasites...
  95. ncbi A family affair: var genes, PfEMP1 binding, and malaria disease
    Susan M Kraemer
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Curr Opin Microbiol 9:374-80. 2006
    ..Detailed understanding of PfEMP1 expression and receptor binding mechanisms during infection and of the antigenic relatedness of disease variants might lead to new approaches in prevention of malaria disease...
  96. pmc Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure
    Robert S Coyne
    J Craig Venter Institute formerly The Institute for Genomic Research, 9704 Medical Center Dr, Rockville, MD, USA
    BMC Genomics 9:562. 2008
    ....
  97. ncbi Early origin and recent expansion of Plasmodium falciparum
    Deirdre A Joy
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0425, USA
    Science 300:318-21. 2003
    ..However, both the world and some regional populations appear to be older (50,000 to 100,000 years old), suggesting an earlier wave of migration out of Africa, perhaps during the Pleistocene migration of human beings...
  98. ncbi Systems biology in malaria research
    Martin J Fraunholz
    Junior Group Applied Bioinformatics and Cell Biology, Institute of Microbiology, Ernst Moritz Arndt University, Friedrich Ludwig Jahn Strasse 15, D 17487 Greifswald, Germany
    Trends Parasitol 21:393-5. 2005
    ..By integrating genomics, transcriptomics and proteomics, the authors can classify and annotate genes by their expression profiles and can even detect evidence of posttranscriptional gene silencing in the murine malaria species...
  99. pmc Detection of genome-wide polymorphisms in the AT-rich Plasmodium falciparum genome using a high-density microarray
    Hongying Jiang
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Genomics 9:398. 2008
    ..Here we evaluate polymorphism detection accuracy of a high-density 'tiling' microarray with 2.56 million probes by comparing single feature polymorphisms (SFP) calls from the microarray with known SNP among parasite isolates...
  100. ncbi Utilization of genomic sequence information to develop malaria vaccines
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Exp Biol 206:3789-802. 2003
    ..Here, we present our strategy to exploit the genomic sequence of P. falciparum for malaria vaccine development...
  101. ncbi Functional genomics of Plasmodium falciparum through transposon-mediated mutagenesis
    Bharath Balu
    Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Cell Microbiol 8:1529-36. 2006
    ..This technique could hence be employed to set up several biological screens in this lethal protozoan parasite that may lead to identification of novel drug targets and vaccine candidates...

Research Grants66

  1. Genome Evolution, Innovation and Adaptation in the Apicomplexa
    Jessica C Kissinger; Fiscal Year: 2010
    ..The results of these studies will be shared with the larger research community via publication and deposition in community databases and Bioinformatics Resource Centers. ..
  2. Novel Anti-Malarials by Combinatorial Pharmacogenomics
    Joseph DeRisi; Fiscal Year: 2006
    ..The compiled relationships from known anti-malarials, novel quinolines, and toxicology will then be used to guide and optimize additional synthesis efforts toward the ultimate goal of producing effective therapeutics. ..
  3. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    ..abstract_text> ..
  4. GENE DISCOVERY IN TOXOPLASMA GONDII
    L Sibley; Fiscal Year: 2003
    ..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
  5. Microfilaments in Giardia Attachment and Virulence
    HEIDI ELMENDORF; Fiscal Year: 2006
    ..This work will advance our understanding of parasite pathogenesis and develop our knowledge of microfilaments as a target for drug design. ..
  6. Calcium ATPases in Apicomplexan Parasites as Potential D
    L Sibley; Fiscal Year: 2006
    ..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
  7. Mucosal Immunity to Toxoplasma gondii
    L Sibley; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  8. Confocal Microscope for Microbiology
    L Sibley; Fiscal Year: 2002
    ..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
  9. Transcriptional Regulation in Giardia lamblia
    HEIDI ELMENDORF; Fiscal Year: 2006
    ..These studies will build an understanding of transcription in Giardia lamblia and lay foundations for study of the role of the regulation of gene expression in disease. ..
  10. GENETIC DISSECTION OF TOXOPLASMA GONDII
    David Roos; Fiscal Year: 2008
    ..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
  11. Adherence of malaria-infected red cells
    Alan Cowman; Fiscal Year: 2009
    ..These studies will contribute to and increased understanding of the pathophysiology of falciparum malaria. ..
  12. MARINER TRANSPOSONS OF MAMMALS
    Hugh Robertson; Fiscal Year: 2001
    ..It will also reveal how occasionally particular copies of transposons can become integral parts of their host genetic complements. ..
  13. Gustatory and olfactory receptors of Anopheles gambiae
    Hugh Robertson; Fiscal Year: 2006
    ..This work will also contribute to our overall understanding of the molecular basis of chemoreception in insects and other animals. [unreadable] [unreadable]..
  14. HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTION
    David Prescott; Fiscal Year: 2001
    ..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
  15. GALACTOSE LECTIN OF E HISTOLYTICA
    Barbara Mann; Fiscal Year: 2002
    ..Successful completion of these experiments will provide insight into the role and identity of PTK/substrates in lectin-mediated killing and may establish the basis for novel therapeutic or prophylactic measures against amebiasis. ..
  16. Regulation of Growth to Differentiation Transitions
    Adam Kuspa; Fiscal Year: 2008
    ..Thus, this work should impact our ability to treat human diseases caused by defects in cellular growth control, such as cancer. ..
  17. TETRAHYMENA GENOME PROJECT
    Eduardo Orias; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  18. Effects of Artemisinin on Leishmania SERCA pump
    JOHN MEADE; Fiscal Year: 2008
    ..cruzi and T. brucei SERCAs. [unreadable] [unreadable] [unreadable]..
  19. Plasmodium Genome Database
    Christian Stoeckert; Fiscal Year: 2008
    ..The overall goal of this project is to develop and maintain a database that expedites biological discovery, including the identification of drug targets and vaccine antigens useful in the fight against malaria. ..
  20. CRYPTOSPORIDIUM PARVUM GENOME SEQUENCING PROJECT (MINN)
    Mitchell Abrahamsen; Fiscal Year: 2002
    ..parvum genome. ..
  21. IMPDH as a Drug Target in Cryptosporidium
    Boris Striepen; Fiscal Year: 2008
    ..This work will provide urgently needed molecular reagents to understand the biology of nutrient uptake and drug resistance in this unique parasite. [unreadable] [unreadable] [unreadable]..
  22. Systems Biology of Plasmodium falciparum: Building and Exploring Network Models
    Yufeng Wang; Fiscal Year: 2007
    ....
  23. Ninth International Congress on Toxoplasmosis
    Boris Striepen; Fiscal Year: 2007
    ..gondii that is not often seen in fields as high paced and[unreadable] competitive and has facilitated rapid and sustained progress in research on this pathogen[unreadable] and related parasites. ..
  24. Redox mechanism of cancer prevention by selenium
    VADIM GLADYSHEV; Fiscal Year: 2002
    ..These approaches should yield insight into the mechanism of the anticancer effect of selenium and may lead to the identification of human cancers most susceptible to selenium chemoprevention. ..
  25. MAPPING DRUG RESISTANCE GENES IN PLASMODIUM FALCIPARUM
    Tim J Anderson; Fiscal Year: 2010
    ..We aim to locate parasite genes underlying resistance. This will allow us to track the spread of resistance, to better understand how antimalarial drugs work, and to redesign drugs to restore their effectiveness. ..
  26. Identification of Transcriptional Regulatory Networks in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2008
    ..We are interested in understanding the molecular mechanisms that the parasite uses to cause disease. [unreadable] [unreadable] [unreadable]..
  27. Comparative Evolutionary Genomics and Infectious Disease
    Jane Carlton; Fiscal Year: 2010
    ....
  28. Innate immunity and dendritic cells in cryptosporidiosis
    SAUL R TZIPORI; Fiscal Year: 2010
    ..The proposed studies will provide the basis for understanding the mechanisms of innate immune recognition and response to parasite invasion necessary for future design of vaccines and other methods of interventions. ..
  29. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2010
    ..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..
  30. Transcription of protein-coding genes in Leishmania
    PETER JOHN MYLER; Fiscal Year: 2010
    ..In this proposal, we will test this hypothesis and build on our current work to further characterize the chromatin-modifying enzymes that are involved in transcription initiation. ..
  31. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2008
    ..Mutations will be mapped to the genome of C. hominis, C. muris and more distantly related apicomplexan to identify those most likely to affect protein function or transcriptional regulation. ..
  32. Intercellular signaling during terminal differentiation
    William Loomis; Fiscal Year: 2009
    ..Together these studies will add to the foundation for improvements in the diagnosis and treatment of neurological diseases. ..
  33. Experimental Annotation of the P. Falciparum Genome
    Elizabeth Winzeler; Fiscal Year: 2009
    ..The basic genomic methods described here should serve as models for determining gene function in other parasites with difficult genetics. ..
  34. PROTEIN PHOSPHORYLATION IN TRYPANOSOMES
    Marilyn Parsons; Fiscal Year: 2010
    ..Together these studies will signficantly expand our knowledge of the network of signaling interactions in trypanosomes, with the goal of identifying new drug and vaccine targets. ..
  35. Trypansoma cruzi-induced host cell signaling responses
    Barbara Burleigh; Fiscal Year: 2009
    ..cruzi infective process. It is the understanding of these basic processes that will guide our efforts toward effective prevention and control of Chagas'disease. ..
  36. Developing Tools to Study Encystation in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2007
    ..We are interested in understanding the life stages of the parasite with the goal of identifying new ways to diagnose, treat, and prevent human infections. [unreadable] [unreadable] [unreadable]..
  37. Specific Human Monoclonal Antibodies for HUS Prophylaxis
    Saul Tzipori; Fiscal Year: 2007
    ..This is not a hypothesis-driven proposal, but an essential segment for the characterization of a promising therapy for HUS, against which currently there is no effective treatment. ..
  38. RNA as the catalyst for screening drugs against trypanosomatids
    Reza Salavati; Fiscal Year: 2006
    ..They will also contribute to development of drugs against trypanosomatids. ..
  39. Functional Genomics of Dictyostelium Development
    William Loomis; Fiscal Year: 2005
    ..Predictions derived from such networks will be tested when microarray analyses are extended to include further developmental mutants. ..
  40. Purine salvage in Cryptosporidium parvum
    Mitchell Abrahamsen; Fiscal Year: 2005
    ..In addition, these studies demonstrate our ability to use heterologous expression of C. parvum genes in yeast as a means to develop a high-throughput screening assay for the identification of specific inhibitors. ..
  41. SEQUENCING THE DICTYOSTELIUM GENOME
    Adam Kuspa; Fiscal Year: 2004
    ..Complete sequence data obtained from experimentally tractable organisms, such as Dictyostelium, will continue to stimulate genomic investigations in many systems, including humans. ..
  42. A Bioinformatic Approach to Inferring Protein Contacts
    MARCELLA MCCLURE; Fiscal Year: 2005
    ..Several experimental laboratories have agreed to test the residue contact predictions resulting from these studies for Ebola, VSV and measles. ..
  43. Chromosome breakage and DNA detection in Tetrahymena
    Meng Chao Yao; Fiscal Year: 2006
    ..abstract_text> ..
  44. Gene Duplication and Genome Evolution
    Austin Hughes; Fiscal Year: 2004
    ..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
  45. RECOMBINANT SHIGA-TOXIN-SPECIFIC HUMAN ANTIBODIES
    Saul Tzipori; Fiscal Year: 2004
    ..abstract_text> ..
  46. Adhesins and Invasins of Francisella Tularensis
    Barbara Mann; Fiscal Year: 2004
    ..Understanding the basic mechanisms of pathogenicity will lead to a greater understanding of why this organism is such an effective pathogen, and the identification of new targets for therapies. ..
  47. IMMUNITY TO CRYPTOSPORIDIOSIS IN SIV INFECTED MACAQUES
    Saul Tzipori; Fiscal Year: 2002
    ..An understanding of this mechanism is important for the derivation of immunotherapeutic strategies that may be necessary to control chronic cryptosporidiosis in patients with AIDS. ..
  48. CHAGAS DISEASE: AUTOIMMUNITY STEMS FROM PARASITE INVASION OF HOST GENOME
    Antonio Teixeira; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  49. 2006 Thiol-based Redox Regulation and Signaling Gordon Research Conference
    VADIM GLADYSHEV; Fiscal Year: 2006
    ..A diverse mix of established and emerging leaders in the field, and of junior and senior investigators will be included in the program. [unreadable] [unreadable] [unreadable]..
  50. TRYPANASOMA CRUZI GENOME-SBRI
    Kenneth Stuart; Fiscal Year: 2004
    ..This information will aid the development on diagnostic, therapeutic, and preventative measures, as well as further our understanding of fundamental molecular phenomena. ..
  51. Host fibrogenic response to Trypanosoma cruzi infection
    Barbara Burleigh; Fiscal Year: 2006
    ..cruzi infection is mediated by a stable soluble parasite factor, which may have important therapeutic potential for a broad range of fibrotic disorders. [unreadable] [unreadable]..
  52. Assessing Genetic Diveristy in Entamoeba Histolytica
    Upinder Singh; Fiscal Year: 2006
    ..This approach will also provide a number of novel genetic targets for the development of improved epidemiological markers, diagnostic assays, and treatment strategies. ..
  53. Forward Genetics in Tetrahymena: Cloning by Complementation
    ROBERT COYNE; Fiscal Year: 2008
    ..Numerous other basic studies with far-reaching relevance to human health would be facilitated by the availability of the proposed freely available resource. [unreadable] [unreadable] [unreadable] [unreadable]..
  54. Genetic Transformation of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
    ..Approach: Transformation by electroporation of sporozoites with GFP expression plasmids; detection of transformants in cell culture by fluorescent microscopy, anti-GFP antibodies, or reverse-transcriptase PCR. ..
  55. Biogenesis of Dense Core Secretory Granules in Tetrahymena
    AARON TURKEWITZ; Fiscal Year: 2009
    ....
  56. MOLECULAR NETWORKS THAT COORDINATE DIFFERENTIATION
    William Loomis; Fiscal Year: 2003
    ..Together these studies will help to establish a signal transduction pathway that can serve as a model for studies in other systems. ..
  57. EGSi, A Tool for Gene Inactivation in Trypanosomatids
    Reza Salavati; Fiscal Year: 2004
    ..abstract_text> ..
  58. Discovery of Allelic Variation in Plasmodium falciparum
    Elizabeth Winzeler; Fiscal Year: 2004
    ..The exploratory work described herein should also be useful in determining whether hybridization-based methods could be used to identify particular isolates of other microbial pathogens, including those that may be used in bioterrorism. ..
  59. A Linkage Map for Schistosoma mansoni
    Tim Anderson; Fiscal Year: 2008
    ..Development of a linkage map will pave the way for finding these genes. [unreadable] [unreadable] [unreadable]..
  60. Comparative Analysis of Editosome in Trypanosomatids
    Reza Salavati; Fiscal Year: 2003
    ..These analyses will be useful for understanding the function of the editosome and its components and also for development of drugs that could be effective for all three species. ..
  61. "Vivax Malaria Research: 2005 and Beyond"
    Jane Carlton; Fiscal Year: 2005
    ..vivax with P. falciparum, epidemiology and diversity studies, vectors and transmission dynamics, and a bioinformatics workshop for researchers and students to learn to manipulate Plasmodium sequence data. ..
  62. Studies on Cryptosporidium parvum Type 1
    Saul Tzipori; Fiscal Year: 2006
    ..The third aim investigates the extent of genetic variation and exchange within and between the two types of C. parvum to determine whether they belong to one or two species. ..
  63. TRYPANOSOMA CRUZI MUCOSAL & SYSTEMIC PROTECTIVE IMMUNITY
    DANIEL HOFT; Fiscal Year: 2008
    ..Chemokine and/or integrin receptor expression will be studied by flow cytometry and RT-Real-Time PCR, and the in vivo effects of defective chemokine and integrin binding and/or signaling will be evaluated. [unreadable] [unreadable]..
  64. Generation of Reagents for E. bieneusi Genome Sequencing
    Saul Tzipori; Fiscal Year: 2003
    ..The outcome of this proposal will lead to a submission of a full proposal to sequence the EB genome. ..
  65. PIG MODEL FOR E BIENEUSI
    Saul Tzipori; Fiscal Year: 2002
    ..Given the cost and nature of the simian SIV model it is unlikely this model would serve the same function. ..