Genomes and Genes
restriction fragment length polymorphism
Summary: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
Publications359 found, 100 shown here
- The role of pfmdr1 in Plasmodium falciparum tolerance to artemether-lumefantrine in AfricaChristin Sisowath
Malaria Research Unit, Division of Infectious Diseases, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden
Trop Med Int Health 12:736-42. 2007..e. pfmdr1 gene amplification, pfmdr1 Y184F, S1034C, N1042D, D1246Y, pfcrt S163R and PfATP6 S769N...
- Sensitivity to antifolates and genetic analysis of Plasmodium vivax isolates from ThailandKanchana Rungsihirunrat
Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand
Am J Trop Med Hyg 76:1057-65. 2007..Results suggest that quadruple mutant alleles confer decreased sensitivity to pyrimethamine but retain sensitivity to WR99210...
- Characterization of the archaeal community in a minerotrophic fen and terminal restriction fragment length polymorphism-directed isolation of a novel hydrogenotrophic methanogenHinsby Cadillo-Quiroz
Department of Microbiology, Cornell University, 270 Wing Hall, Ithaca, NY 14853, USA
Appl Environ Microbiol 74:2059-68. 2008..and Methanosaetaceae were the codominant groups in all libraries and results of terminal restriction fragment length polymorphism (T-RFLP) analysis...
- Comparison of bacterial communities in New England Sphagnum bogs using terminal restriction fragment length polymorphism (T-RFLP)Sergio E Morales
Department of Microbiology and Molecular Genetics, University of Vermont, 95 Carrigan Drive, Burlington, VT 05405, USA
Microb Ecol 52:34-44. 2006..Several other phyla were represented, as well as two Candidate Division-level taxonomic groups. These data suggest that bog microbial communities are complex, possibly stratified, and similar among multiple sites...
- Frequency of embB codon 306 mutations in ethambutol-susceptible and -resistant clinical Mycobacterium tuberculosis isolates in KuwaitS Ahmad
Department of Microbiology, Kuwait University, P O Box 24923, Safat 13110, Kuwait
Tuberculosis (Edinb) 87:123-9. 2007..Recent reports of embB306 mutations in ethambutol-resistant and ethambutol-susceptible drug-resistant strains of Mycobacterium tuberculosis have questioned the significance of these mutations in conferring resistance to ethambutol (EMB)...
- Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosisPhilip Supply
INSERM U629, Institut Pasteur de Lille, 1, Rue du Prof Calmette, F 59019 Lille cedex, France
J Clin Microbiol 44:4498-510. 2006..tuberculosis transmission was found to be equal to that of IS6110 restriction fragment length polymorphism typing, as shown in a companion population-based study...
- Genetic diversity and multiple infections of Plasmodium vivax malaria in Western ThailandLiwang Cui
Department of Entomology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
Am J Trop Med Hyg 68:613-9. 2003..8%, 18.7%, and 6.5%, respectively. The 13 alleles distinguished by restriction fragment length polymorphism analysis did not show a significant seasonal variation in frequency...
- Formation of pseudo-terminal restriction fragments, a PCR-related bias affecting terminal restriction fragment length polymorphism analysis of microbial community structureMarkus Egert
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg Lahn, Germany
Appl Environ Microbiol 69:2555-62. 2003Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified genes is a widely used fingerprinting technique in molecular microbial ecology...
- Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosisMara Cardoso Oelemann
INSERM, U629, Institut Pasteur de Lille, Lille, France
J Clin Microbiol 45:691-7. 2007..Of the 154 isolates investigated, more than 90% had high IS6110 copy numbers (>/=6). IS6110 restriction fragment length polymorphism (RFLP) typing resulted in 13 clusters, 5 of which had a confirmed epidemiological link...
- High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiologyE Mazars
, , U447, Institut Pasteur de Lille, rue du Prof. Calmette, F-59019 Lille Cedex, France
Proc Natl Acad Sci U S A 98:1901-6. 2001..The current standard for Mycobacterium tuberculosis typing based on IS6110 restriction fragment length polymorphism (RFLP) suffers from the difficulty of comparing data between independent laboratories...
- Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes to characterize microbial communitiesUrsel M E Schütte
Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA
Appl Microbiol Biotechnol 80:365-80. 2008Terminal restriction fragment length polymorphism (T-RFLP) analysis is a popular high-throughput fingerprinting technique used to monitor changes in the structure and composition of microbial communities...
- Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysisJudith Rohde
Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany
J Clin Microbiol 40:2598-600. 2002A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B...
- MiCA: a web-based tool for the analysis of microbial communities based on terminal-restriction fragment length polymorphisms of 16S and 18S rRNA genesConrad Shyu
Department of Computer Science, University of Idaho, Moscow, ID 83844, USA
Microb Ecol 53:562-70. 2007..APLAUS estimates the relative abundances of populations and reports a listing of phylotypes that are consistent with the empirical data. MiCA is accessible at http://mica.ibest.uidaho.edu/...
- A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene ampliconsGuo bing Zeng
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
World J Gastroenterol 10:3132-6. 2004Traditional hepatitis B virus (HBV) genotyping methods using restriction fragment length polymorphism (RFLP) can reliably identify genotypes A to F...
- Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphismHidenori Hayashi
Microbe Division Japan Collection of Microorganisms, RIKEN BioResource Center, Saitama 351 0198, Japan
J Med Microbiol 54:1093-101. 2005..three elderly individuals at autopsy were compared using 16S rRNA gene libraries and terminal restriction fragment length polymorphism (T-RFLP)...
- Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase geneA F Norman
Department of Microbiology, University of Georgia, Athens, USA
J Clin Microbiol 33:1797-803. 1995..henselae primers and analyzed for restriction fragment length polymorphism. The resulting patterns for all 28 isolates were similar or identical to that of the recognized B...
- Application of a new PCR primer for terminal restriction fragment length polymorphism analysis of the bacterial communities in plant rootsMasao Sakai
Department of Plant Resources, Faculty of Agriculture, Kyushu University, 6 10 1 Hakozaki, Higashi, Fukuoka 812 8581, Japan
J Microbiol Methods 59:81-9. 2004..To confirm how useful the community analysis of rhizobacteria is using 783r, the terminal restriction fragment length polymorphism (T-RFLP) method was performed with wheat (Triticum aestivum) and spinach (Spinacea oleracea) root ..
- Association of genetic mutations in Plasmodium vivax dhfr with resistance to sulfadoxine-pyrimethamine: geographical and clinical correlatesM Imwong
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Antimicrob Agents Chemother 45:3122-7. 2001..Functional polymorphisms in the dhfr gene of P. vivax (pvdhfr) were assessed by PCR-restriction fragment length polymorphism using blood samples taken from 125 patients with acute vivax malaria from three widely separated ..
- Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpAV Roux
Unite des Rickettsies, Faculte de Medecine, Centre National de la Recherche Scientifique EPJ 0054, Marseille, France
J Clin Microbiol 34:2058-65. 1996Currently, the genotypic identification of the spotted fever group (SFG) rickettsiae is based on restriction fragment length polymorphism analysis of PCR-amplified genes coding for the enzyme citrate synthase and the surface proteins ..
- Single-nucleotide polymorphism-based population genetic analysis of Mycobacterium tuberculosis strains from 4 geographic sitesMichaela M Gutacker
Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
J Infect Dis 193:121-8. 2006..The SNP-based phylogenetic framework provides new insight into the worldwide evolution of M. tuberculosis and a gateway for investigating genotype-disease phenotype relationships in large samples of strains...
- Development of a species-specific PCR-restriction fragment length polymorphism analysis procedure for identification of Madurella mycetomatisA O Ahmed
Institute of Endemic Diseases, University of Khartoum, Sudan, The Netherlands
J Clin Microbiol 37:3175-8. 1999..mycetomatis, ITS amplicons were obtained from 25 different clinical isolates and subjected to restriction fragment length polymorphism (RFLP) analysis with CfoI, HaeIII, MspI, Sau3AI, RsaI, and SpeI restriction enzymes...
- Identification of 5 types of Cryptosporidium parasites in children in Lima, PeruL Xiao
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341 3717, USA
J Infect Dis 183:492-7. 2001..Contrary to common belief, novel Cryptosporidium species and C. parvum genotypes can infect HIV-negative children...
- Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samplesSimone M Caccio
Laboratory of Parasitology, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
Int J Parasitol 32:1023-30. 2002..Finally, a simple PCR-restriction fragment length polymorphism method was developed for the rapid discrimination of Assemblages and applied for the direct ..
- Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphismChiao Tang Chang
Department of Medical Research, Yuan s General Hospital, Kaohsiung, Taiwan, Republic of China
Appl Environ Microbiol 68:3159-61. 2002..The 10 NTM isolates were also identified to the species level using PCR-restriction fragment length polymorphism. Our findings indicate that NTM in hospital tap water are the possible cause of false positives ..
- Terminal restriction fragment length polymorphism data analysis for quantitative comparison of microbial communitiesChristopher B Blackwood
Center for Microbial Ecology and Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824, USA
Appl Environ Microbiol 69:926-32. 2003Terminal restriction fragment length polymorphism (T-RFLP) is a culture-independent method of obtaining a genetic fingerprint of the composition of a microbial community...
- Comparison of PCR-RFLP and Genescan-based genotyping for analyzing infection dynamics of Plasmodium falciparumNicole Falk
Swiss Tropical Institute, Basel, Switzerland
Am J Trop Med Hyg 74:944-50. 2006..protein 2: 1) fluorescence-labeled polymerase chain reaction (PCR) and GeneScan-sizing and 2) restriction fragment length polymorphism (RFLP)...
- characterization of bacterial community diversity in cystic fibrosis lung infections by use of 16s ribosomal DNA terminal restriction fragment length polymorphism profilingG B Rogers
Division of Life Sciences, King s College London, London SE1 9NN, UK
J Clin Microbiol 42:5176-83. 2004..In conclusion, this reveals the enormous complexity of bacteria within the CF lung. Although their significance is yet to be determined, these findings alter our perception of CF lung infections...
- Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and 'M. avium subsp. hominissuis' for the human/porcine type of M. aviumWouter Mijs
Innogenetics N V, Ghent, Belgium
Int J Syst Evol Microbiol 52:1505-18. 2002..For clarity in the epidemiology of M. avium-related disease, isolates from humans and pigs with multibanded IS1245 RFLPs merit a separate designation. The designation 'M. avium subsp. hominissuis' is suggested for this group of bacteria...
- Evaluation of spoligotyping in a study of the transmission of Mycobacterium tuberculosisY O Goguet de la Salmonière
Unite de Genetique Mycobacterienne, Institut Pasteur, Paris, France
J Clin Microbiol 35:2210-4. 1997..G. Torrea et al., J. Clin. Microbiol. 34:1043-1049, 1996) by IS6110-based restriction fragment length polymorphism (RFLP) analysis...
- Targeting the rpoB gene using nested PCR-restriction fragment length polymorphism for identification of nontuberculous mycobacteria in hospital tap waterJi Hyun Shin
Division of Bacterial Respiratory Infection, Center for Infectious Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul, 122 701, Republic of Korea
J Microbiol 46:608-14. 2008..Two-step direct PCR targeting the rpoB gene, restriction fragment length polymorphism (RFLP) using the MspI restriction enzyme, and sequence analysis were performed for identification ..
- DNA repair gene polymorphisms, bulky DNA adducts in white blood cells and bladder cancer in a case-control studyG Matullo
Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Turin, Italy
Int J Cancer 92:562-7. 2001..Our results suggest that bladder-cancer risk can be genetically modulated by XRCC3, which may repair DNA cross-link lesions produced by aromatic amines and other environmental chemicals...
- Community structure of denitrifiers, bacteria, and archaea along redox gradients in Pacific Northwest marine sediments by terminal restriction fragment length polymorphism analysis of amplified nitrite reductase (nirS) and 16S rRNA genesG Braker
Center for Microbial Ecology, 540 Plant and Soil Sciences Building, Michigan State University, East Lansing, MI 48824 1325, USA
Appl Environ Microbiol 67:1893-901. 2001..and bacterial and archaeal 16S rRNA genes (rDNAs) were PCR amplified and analyzed by terminal restriction fragment length polymorphism (T-RFLP) analysis...
- Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NMP I Fields
Foodborne and Diarrheal Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 35:1066-70. 1997..To further characterize nonmotile isolates (designated NM), we developed a PCR-restriction fragment length polymorphism (PCR-RFLP) test to identify and characterize the gene encoding the H antigen (fliC) in E. coli...
- Merozoite surface protein-3alpha is a reliable marker for population genetic analysis of Plasmodium vivaxSedigheh Zakeri
Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
Malar J 5:53. 2006..In this study, msp-3alpha was compared and assessed with msp-1 marker in order to find whether msp-3alpha is a reliable genetic marker for P. vivax population studies...
- Genetic diversity of multidrug-resistant Mycobacterium tuberculosis isolates and identification of 11 novel rpoB alleles in TaiwanRuwen Jou
Reference Laboratory of Mycobacteriology, Center for Disease Control, Department of Health, Taiwan, 161 Kun Yang Street, Nan Kang, Taipei 115, Taiwan
J Clin Microbiol 43:1390-4. 2005..5% were found to belong to the Beijing family on the basis of spoligotyping results. IS6110 restriction fragment length polymorphism fingerprinting showed genetic diversity among the multidrug-resistant isolates. Furthermore, 90...
- Determination of genotypic diversity of Mycobacterium avium subspecies from human and animal origins by mycobacterial interspersed repetitive-unit-variable-number tandem-repeat and IS1311 restriction fragment length polymorphism typing methodsNicolas Radomski
Unité Zoonoses Bactériennes, LERPAZ, AFSSA, 23 Avenue du Général de Gaulle, Maisons Alfort F 94706 Cedex, France
J Clin Microbiol 48:1026-34. 2010..885, which is almost equal to that obtained by IS1311 restriction fragment length polymorphism (RFLP) typing as a reference...
- Stability and change in estuarine biofilm bacterial community diversityJoseph A Moss
Center for Environmental Diagnostics and Bioremediation, University of West Florida, Pensacola, FL 32514, USA
Appl Environ Microbiol 72:5679-88. 2006..Community profiling was achieved by using 16S rRNA genes and terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes in combination with ribotyping, cloning, and sequencing to evaluate ..
- Diversity of Staphylococcus species strains based on partial kat (catalase) gene sequences and design of a PCR-restriction fragment length polymorphism assay for identification and differentiation of coagulase-positive species (S. aureus, S. delphini, S. Giuseppe Blaiotta
Department of Food Science, School of Agriculture, University of Naples Federico II, Via Universita 100, 80055 Portici, Italy
J Clin Microbiol 48:192-201. 2010..gene could be exploited for rapid, low-cost identification of staphylococcal species through PCR-restriction fragment length polymorphism (RFLP) analysis...
- Statistical assessment of variability of terminal restriction fragment length polymorphism analysis applied to complex microbial communitiesPierre Rossi
Ecole Polytechnique Federale de Lausanne, ENAC ISTE Laboratory for Environmental Biotechnology, CH C3 425, Station 6, CH 1015 Lausanne, Switzerland
Appl Environ Microbiol 75:7268-70. 2009..Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a high level of reproducibility...
- Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolatesCaroline Allix-Béguec
Genoscreen, 1, rue du Professeur Calmette, Lille 59019 Cedex, France
J Clin Microbiol 46:2692-9. 2008..pattern, the single-nucleotide- and large-sequence-polymorphism profiles, and the IS6110 restriction fragment length polymorphism fingerprint...
- Epidemiological evidence of the spread of a Mycobacterium tuberculosis strain of the Beijing genotype on Gran Canaria IslandJ A Caminero
Service of Pneumology, Emergency Department, University General Hospital Dr Negrín, Las Palmas de Gran Canaria, Spain
Am J Respir Crit Care Med 164:1165-70. 2001..IS6110 restriction fragment length polymorphism (RFLP) analysis was performed on M. tuberculosis isolates from 566 of the 721 patients (78...
- Effects of genetic variability of Mycobacterium tuberculosis strains on the presentation of diseaseAeesha N J Malik
Hospital for Tropical Diseases, London, UK
Lancet Infect Dis 5:174-83. 2005..This review discusses molecular epidemiology and genetic studies, and their contribution to the understanding of the links between genotypic and phenotypic characteristics of M tuberculosis strains...
- Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 geneF Brunello
Dipartimento di Patologia, Sezione di Microbiologia, , Italy
J Clin Microbiol 39:2799-806. 2001..strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein...
- Frequency of single nucleotide polymorphisms in the P-glycoprotein drug transporter MDR1 gene in white subjectsI Cascorbi
Institute of Clinical Pharmacology, University Medical Center Charite, Humboldt University Berlin
Clin Pharmacol Ther 69:169-74. 2001..Various polymorphisms in the MDR1 gene were recently identified. A silent mutation in exon 26 (C3435T) was correlated with intestinal P-glycoprotein expression and oral bioavailability of digoxin...
- Genetic structure of Plasmodium vivax isolates from two malaria endemic areas in AfghanistanSedigheh Zakeri
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Acta Trop 113:12-9. 2010..PCR/RFLP products with Hha I and Alu I were detected 52 and 38 distinct variants among Nangarhar and Herat isolates, respectively. These results strongly indicate that the P. vivax populations in Afghanistan are highly diverse...
- Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriaeT D Leser
Danish Veterinary Laboratory, Copenhagen V, Denmark
Appl Environ Microbiol 66:3290-6. 2000Bacterial communities in the large intestines of pigs were compared using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting the 16S ribosomal DNA...
- Identification and characterization of transmissible Pseudomonas aeruginosa strains in cystic fibrosis patients in England and WalesFiona W Scott
Laboratory of HealthCare Associated Infection, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK
J Med Microbiol 53:609-15. 2004..These results emphasize the need for continued surveillance of P. aeruginosa genotypes in CF patients to provide informed infection control policy in treatment centres...
- Mitochondrial DNA variations in Russian and Belorussian populationsOlga Belyaeva
Institute of Molecular Genetics of RAS, Moscow, Russia
Hum Biol 75:647-60. 2003..These data should contribute to general studies of mitochondrial DNA variations in Europe...
- A soybean transcript map: gene distribution, haplotype and single-nucleotide polymorphism analysisIk Young Choi
Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville, Maryland 20705, USA
Genetics 176:685-96. 2007....
- Using terminal restriction fragment length polymorphism (T-RFLP) to identify mycorrhizal fungi: a methods reviewI A Dickie
Landcare Research, P O Box 40, Lincoln 7640, New Zealand
Mycorrhiza 17:259-70. 2007Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology...
- Application of new primer-enzyme combinations to terminal restriction fragment length polymorphism profiling of bacterial populations in human fecesKoji Nagashima
Hokkaido Food Processing Research Center, Ebetsu, Hokkaido 069 0836, Japan
Appl Environ Microbiol 69:1251-62. 2003New primer-enzyme combinations for terminal restriction fragment length polymorphism (T-RFLP) targeting of the 16S rRNA gene were constructed by using the T-RFLP analysis program (designated TAP T-RFLP) located at the Ribosomal Database ..
- Comparative genotyping of Campylobacter jejuni by amplified fragment length polymorphism, multilocus sequence typing, and short repeat sequencing: strain diversity, host range, and recombinationLeo M Schouls
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands
J Clin Microbiol 41:15-26. 2003....
- Discordance between mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing and IS6110 restriction fragment length polymorphism genotyping for analysis of Mycobacterium tuberculosis Beijing strains in a setting of high incidence ofM Hanekom
Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, P O Box 19063, Tygerberg, South Africa 7505
J Clin Microbiol 46:3338-45. 2008IS6110 restriction fragment length polymorphism (RFLP) genotyping is the most widely used genotyping method to study the epidemiology of Mycobacterium tuberculosis...
- An integrated restriction fragment length polymorphism--amplified fragment length polymorphism linkage map for cultivated sunflowerM A Gedil
Department of Crop and Soil Science, Oregon State University, Corvallis 97331-3002, USA
Genome 44:213-21. 2001b>Restriction fragment length polymorphism (RFLP) maps have been constructed for cultivated sunflower (Helianthus annuus L.) using three independent sets of RFLP probes...
- Prevalence of Mycobacterium avium in slaughter pigs in The Netherlands and comparison of IS1245 restriction fragment length polymorphism patterns of porcine and human isolatesR E Komijn
National Inspection Service for Livestock and Meat, 2270 JA Voorburg, The Netherlands
J Clin Microbiol 37:1254-9. 1999..2%). Ninety-one of the isolated strains were analyzed by restriction fragment length polymorphism (RFLP) typing with insertion sequence IS1245 as a probe...
- Cyclin D1 overexpression in bronchial epithelia of patients with lung cancer is associated with smoking and predicts survivalD Ratschiller
Institute of Medical Oncology, University Hospital of Bern, 3010 Bern, Switzerland
J Clin Oncol 21:2085-93. 2003..Cyclin D1 is overexpressed in almost 60% of resectable non-small-cell lung cancer (NSCLC). In the absence of cyclin D1 gene amplification, overexpression is characterized by allelic imbalanced transcript levels...
- Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extractsTillmann Lueders
Max Planck Institut fur terrestrische Mikrobiologie, D 35043 Marburg, Germany
Appl Environ Microbiol 69:320-6. 2003Terminal restriction fragment length polymorphism (T-RFLP) analysis is a widely used method for profiling microbial community structure in different habitats by targeting small-subunit (SSU) rRNA and also functional marker genes...
- Erythrocyte CR1 expression level does not correlate with a HindIII restriction fragment length polymorphism in Africans; implications for studies on malaria susceptibilityJ A Rowe
Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, UK
Genes Immun 3:497-500. 2002..populations is linked to high (H) and low (L) expression alleles identified by a HindIII restriction fragment length polymorphism (RFLP)...
- Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimensA Nemec
National Institute of Public Health, Prague, Czech Republic
Int J Syst Evol Microbiol 51:1891-9. 2001..Clinical and epidemiological data indicate that A. ursingii has the capacity to cause bloodstream infections in hospitalized patients...
- Epidemiological study of Mycoplasma pneumoniae infections in japan based on PCR-restriction fragment length polymorphism of the P1 cytadhesin geneT Sasaki
Department of Safety Research on Biologics, National Institute of Health, Tokyo
J Clin Microbiol 34:447-9. 1996..the past 20 years in Japan were classified into two groups (I and II) based upon different PCR-restriction fragment length polymorphism patterns of their P1 cytadhesin genes. Clear shifts between the M...
- PCR restriction fragment length polymorphism analysis (PRA)-algorithm targeting 644 bp Heat Shock Protein 65 (hsp65) gene for differentiation of Mycobacterium sppHong Kim
Department of Microbiology and Liver Research Institute, College of Medicine, Seoul National University, 28 Yongon Dong, Chongno Gu, Seoul 110 799, South Korea
J Microbiol Methods 62:199-209. 2005A method based on PCR-restriction fragment length polymorphism analysis (PRA) using a novel region of the hsp65 gene was developed for the rapid and exact identification of mycobacteria to the species level...
- RHD gene deletion occurred in the Rhesus boxF F Wagner
Abteilung Transfusionsmedizin, Universitatsklinikum Ulm, Ulm, Germany
Blood 95:3662-8. 2000..PCR with sequence-specific priming (PCR-SSP) and PCR with restriction fragment length polymorphism (PCR-RFLP) were used for specific detection of the RHD deletion...
- Selective G to T mutations of p53 gene in hepatocellular carcinoma from southern AfricaB Bressac
Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Charlestown 02129
Nature 350:429-31. 1991..This mutation specificity could reflect exposure to a specific carcinogen, one candidate being aflatoxin B1 (ref. 7), a food contaminant in Africa, which is both a mutagen that induces G to T substitution and a liver-specific carcinogen...
- Vaccine-induced immunity circumvented by typical Mycobacterium tuberculosis Beijing strainsKristin Kremer
National Institute for Public Health and the Environment, Bilthoven, The Netherlands
Emerg Infect Dis 15:335-9. 2009..The strains' associations with drug resistance, M. bovis BCG vaccination, and patient characteristics were assessed. BCG vaccination may have positively selected the prevalent typical Beijing strains...
- Major Mycobacterium tuberculosis lineages associate with patient country of originMichael B Reed
Department of Medicine, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
J Clin Microbiol 47:1119-28. 2009..the LSP analysis, we also demonstrate that "clustered" tuberculosis (TB) cases defined through restriction fragment length polymorphism (RFLP) analysis (for isolates with >or=6 IS6110 copies) or RFLP in combination with ..
- Genotyping of Toxoplasma gondii strains from immunocompromised patients reveals high prevalence of type I strainsA Khan
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
J Clin Microbiol 43:5881-7. 2005..The multiplex nested PCR analysis described here will be useful for analyzing the contribution of parasite genotype to toxoplasmosis...
- Comparison of a variable-number tandem-repeat (VNTR) method for typing Mycobacterium avium with mycobacterial interspersed repetitive-unit-VNTR and IS1245 restriction fragment length polymorphism typingTakayuki Inagaki
Department of Microbiology, Faculty of Pharmacy, Meijo University, Tempaku ku, Nagoya, Aichi, Japan
J Clin Microbiol 47:2156-64. 2009..this study, the usefulness of this MATR-VNTR typing method was compared with that of the IS1245-restriction fragment length polymorphism (IS1245-RFLP) typing method and a mycobacterial interspersed repetitive-unit (MIRU)-VNTR typing ..
- Web-based primer design for single nucleotide polymorphism analysisMichael M Neff
Dept of Biology, Washington University, Campus Box 1137, St Louis, MO 63130 4899, USA
Trends Genet 18:613-5. 2002..Here we describe a web-based program, dCAPS Finder 2.0, that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site relative to the polymorphism being analyzed...
- Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphismS D'Amelio
Institute of Parasitology, University of Rome La Sapienza, Rome, Italy
Int J Parasitol 30:223-6. 2000Polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rDNA, for the identification of the three sibling species of the Anisakis simplex complex and morphologically ..
- Genotypic and phenotypic heterogeneity among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patientsIsdore Chola Shamputa
Mycobacteriology Unit, Department of Microbiology, Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerp, Belgium
J Clin Microbiol 42:5528-36. 2004..were screened for heterogeneity and detectable mixed infections by spoligotyping, IS6110-based restriction fragment length polymorphism analysis, and mycobacterial interspersed repetitive unit-variable number of tandem repeat typing...
- Discrimination of seven Anopheles species from San Pedro de Uraba, Antioquia, Colombia, by polymerase chain reaction-restriction fragment length polymorphism analysis of its sequencesMario A Zapata
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia
Am J Trop Med Hyg 77:67-72. 2007..We describe polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of internal transcribed spacer 2 (ITS2) sequences for differentiation of seven ..
- DNA-repair genetic polymorphisms and breast cancer riskTasha R Smith
Department of Cancer Biology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
Cancer Epidemiol Biomarkers Prev 12:1200-4. 2003..We provide evidence that variants of XRCC1, XRCC3, and ERCC4/XPF genes, particularly in combination, contribute to breast cancer susceptibility...
- Molecular characterization of cryptosporidium oocysts in samples of raw surface water and wastewaterL Xiao
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Appl Environ Microbiol 67:1097-101. 2001..In this study, we used a small-subunit rRNA-based PCR-restriction fragment length polymorphism (RFLP) technique to detect and characterize Cryptosporidium oocysts in 55 samples of raw surface ..
- Linkage disequilibrium between minisatellite loci supports clonal evolution of Mycobacterium tuberculosis in a high tuberculosis incidence areaPhilip Supply
Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, Cedex, France
Mol Microbiol 47:529-38. 2003..This linkage disequilibrium was also evident when the MIRU-VNTR types were compared with the IS6110 restriction fragment length polymorphism types. These results support a predominant clonal evolution of M. tuberculosis.
- MDR1 pharmacogenetics: frequency of the C3435T mutation in exon 26 is significantly influenced by ethnicityM M Ameyaw
University of Aberdeen, Department of Medicine and Therapeutics, Institute of Medical Sciences, Foresterhill, Aberdeen, UK
Pharmacogenetics 11:217-21. 2001..from 10 different ethnic groups were evaluated for this SNP using the polymerase chain reaction-restriction fragment length polymorphism assay and the genotype and allele frequency for each group were ascertained...
- Phylogenetic reconstruction within Mycobacterium tuberculosis Beijing genotype in northwestern RussiaIgor Mokrousov
Laboratory of Molecular Microbiology Pasteur Institute, St Petersburg, 197101, Russia
Res Microbiol 153:629-37. 2002..The atypical Beijing strains (4%) are evolutionary older; they probably had a common (unknown) ancestor with typical Beijing strains...
- Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markersKristin Kremer
Mycobacteria Reference Unit, Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, P O Box 1, 3720 BA Bilthoven, The Netherlands
J Clin Microbiol 42:4040-9. 2004..strains, including W strains, have been characterized by their highly similar multicopy IS6110 restriction fragment length polymorphism (RFLP) patterns, deletion of spacers 1 to 34 in the direct repeat region (Beijing spoligotype), ..
- Microbial community structure in midgut and hindgut of the humus-feeding larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae)Markus Egert
Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany
Appl Environ Microbiol 69:6659-68. 2003..Clone libraries and terminal restriction fragment length polymorphism analysis of 16S rRNA genes revealed that the intestines of P...
- Distribution of Mycobacterium avium complex isolates in tissue samples of pigs fed peat naturally contaminated with mycobacteria as a supplementLudmila Matlova
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
J Clin Microbiol 43:1261-8. 2005..intracellulare isolates, and 12 environmentally derived mycobacterial isolates). IS1245 restriction fragment length polymorphism (RFLP) types with >20 bands of 45 distinct RFLP types were found in 49 M. avium subsp...
- New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communitiesCatherine A Osborne
Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
Appl Environ Microbiol 72:1270-8. 2006Terminal restriction fragment length polymorphism (T-RFLP) analysis has the potential to be useful for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables...
- Lactobacillus strain diversity based on partial hsp60 gene sequences and design of PCR-restriction fragment length polymorphism assays for species identification and differentiationGiuseppe Blaiotta
Department of Food Science, School of Agriculture, The University of Naples Federico II, Via Universita 100, 80055 Portici, Italy
Appl Environ Microbiol 74:208-15. 2008..endonuclease analysis of all 499-bp hsp60 partial sequences and we showed that the PCR-restriction fragment length polymorphism (RFLP) patterns obtainable by using both endonucleases AluI and TacI (in separate reactions) can ..
- Genetic polymorphism of cytochrome P4501A1 and susceptibility to oral squamous cell carcinoma and oral precancer lesions associated with smoking/betel useShou Yen Kao
Department of Dentistry, Oral and Maxillofacial Surgery, Taipei Veterans General Hospital, Taiwan, ROC
J Oral Pathol Med 31:505-11. 2002..It is also important to evaluate the association between CYP1A1 polymorphisms and susceptibility of oral precancerous lesion (OPL) to confirm the findings in OSCC cases...
- Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism mapS R Wicks
The Hubrecht Laboratory and Center for Biomedical Genetics. Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Nat Genet 28:160-4. 2001..The usefulness of this approach is illustrated by the rapid mapping of the dyf-5 gene...
- A new mtDNA mutation associated with Leber hereditary optic neuroretinopathyK Huoponen
Department of Medical Genetics, University of Turku, Finland
Am J Hum Genet 48:1147-53. 1991..None of the families with the nt 3460 mutation in ND1 had the previously reported nt 11778 mutation in the ND4 gene. The G-to-A change at nt 3460 is the second mutation so far detected in LHON...
- Relapses of Plasmodium vivax infection usually result from activation of heterologous hypnozoitesMallika Imwong
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
J Infect Dis 195:927-33. 2007..Thus, reappearance of parasitemia after treatment can result from relapse, recrudescence, or reinfection. It has been assumed that parasites causing relapse would be a subset of the parasites that caused the primary infection...
- Mycobacterium tuberculosis spoligotypes in Monterrey, MexicoCarmen A Molina-Torres
Servicio de Dermatologia, Hospital Universitario José E González, Madero y Gonzalitos, Col Mitras Centro, Monterrey, N L CP 64460 Mexico
J Clin Microbiol 48:448-55. 2010..in the group of multidrug-resistant isolates, 37 of them were analyzed by IS6110-based restriction fragment length polymorphism assays, and scarce clustering of strains with more than five bands was observed...
- The population structure of Plasmodium falciparum and Plasmodium vivax during an epidemic of malaria in the Eastern Highlands of Papua New GuineaIvo Mueller
PNG Institute of Medical Research, Goroka, Papua New Guinea
Am J Trop Med Hyg 67:459-64. 2002..This striking difference in genetic diversity suggests endemic, low-level local transmission for P. vivax but an outside introduction of P. falciparum as the most likely source of the epidemic...
- Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification productsT L Marsh
Center for Microbial Ecology, Department of Microbiology, Michigan State University, East Lansing, MI 48824, USA
Curr Opin Microbiol 2:323-7. 1999Terminal restriction fragment length polymorphism is a recent molecular approach that can assess subtle genetic differences between strains as well as provide insight into the structure and function of microbial communities...
- Effects on sister chromatid exchange frequency of polymorphisms in DNA repair gene XRCC1 in smokersYu Chen Lei
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, No 1 Ren Ai Road, Sec 1, 10018 Taipei, ROC
Mutat Res 519:93-101. 2002..mEH (exons 3 and 4), XRCC1 (codon 399) genotypes were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR/RFLP). Smokers had higher SCE frequency than non-smokers (8.4 versus 7.1, P<0.05)...
- T-Align, a web-based tool for comparison of multiple terminal restriction fragment length polymorphism profilesCindy J Smith
Department of Zoology, University College Dublin, Dublin 4, Ireland
FEMS Microbiol Ecol 54:375-80. 2005Terminal restriction fragment length polymorphism (tRFLP) is a potentially high-throughput method for the analysis of complex microbial communities...
- TRiFLe, a program for in silico terminal restriction fragment length polymorphism analysis with user-defined sequence setsPilar Junier
Environmental Microbiology Laboratory, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
Appl Environ Microbiol 74:6452-6. 2008..TRiFLE was used for analyzing T-RFLP data generated for the amoA and pmoA genes. The peaks identified in the T-RFLP patterns show an overlap of ammonia- and methane-oxidizing bacteria in the metalimnion of a subtropical lake...
- Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implicationsG Wang
Department of Medical Microbiology, Academic Medical Centre, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Clin Microbiol Rev 12:633-53. 1999..randomly amplified polymorphic DNA fingerprinting analysis, species-specific PCR and PCR-based restriction fragment length polymorphism (RFLP) analysis, and sequence analysis of 16S rRNA and other conserved genes...
- Evaluation of LSU rRNA-gene PCR primers for analysis of arbuscular mycorrhizal fungal communities via terminal restriction fragment length polymorphism analysisDaniel L Mummey
Microbial Ecology Program, Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA
J Microbiol Methods 70:200-4. 2007..Analysis of both public database and site-specific derived DNA sequences suggesting LSU rDNA-based T-RFLP analysis represents a valuable alternative for analysis of AMF communities...
- Comparison of two target genes for detection and genotyping of Giardia lamblia in human feces by PCR and PCR-restriction fragment length polymorphismIsabelle Bertrand
LCPME UMR 7564 CNRS UHP, Equipe Microbiologie et Physique, Faculte de Pharmacie, 5 rue Albert Lebrun, BP 80 403, 54001 Nancy Cedex, France
J Clin Microbiol 43:5940-4. 2005..G. lamblia cysts isolated from human feces were also analyzed with two previously described PCR-restriction fragment length polymorphism (RFLP) assays, which are based on the detection of tpi or gdh genes...
- Genetic diversity of Plasmodium vivax in Kolkata, IndiaJung Ryong Kim
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Malar J 5:71. 2006..There has been limited information on the genotypic polymorphism of P. vivax in this malaria endemic area. Three highly polymorphic and single copy genes were selected for a study of genetic diversity in Kolkata strains...
- Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humansWenlan Zhang
Institut fur Hygiene, Universitatsklinikum Munster, Germany
J Clin Microbiol 40:1441-6. 2002..We designated this variant "Stx1c" and developed stxB(1) restriction fragment length polymorphism and stx(1c)-specific PCR strategies to determine the frequency and distribution of stx(1c) among ..
- T-RFPred: a nucleotide sequence size prediction tool for microbial community description based on terminal-restriction fragment length polymorphism chromatogramsAntonio Fernandez-Guerra
Department of Continental Ecology Biogeodynamics and Biodiversity Interactions, Centre d Estudis Avançats de Blanes, CSIC, E 17300 Blanes, Spain
BMC Microbiol 10:262. 2010Terminal-Restriction Fragment Length Polymorphism (T-RFLP) is a technique used to analyze complex microbial communities...
- Rapid identification of mycobacteria from smear-positive sputum samples by nested PCR-restriction fragment length polymorphism analysisTsu Lan Wu
Department of Medical Biotechnology and Laboratory Science, Chang Gung University, No 259, Wen Hua 1st Road, Kwei Shan, Tao Yuan 333, Taiwan, Republic of China
J Clin Microbiol 46:3591-4. 2008..A nested PCR-restriction fragment length polymorphism analysis (nested-PRA) assay that focuses on the analysis of the hsp65 gene was developed and ..
- The detection of monkeypox in humans in the Western HemisphereKurt D Reed
Department of Pathology, Marshfield Clinic, Marshfield, Wisc, USA
N Engl J Med 350:342-50. 2004..Zoonotic transmission of a bacterial or viral pathogen was suspected...
- Determining cystic fibrosis-affected lung microbiology: comparison of spontaneous and serially induced sputum samples by use of terminal restriction fragment length polymorphism profilingGeraint B Rogers
King s College London, Molecular Microbiology Research Laboratory, Pharmaceutical Science Division, 150 Stamford Street, Franklin Wilkins Building, London SE1 9NH, United Kingdom
J Clin Microbiol 48:78-86. 2010..Terminal restriction fragment length polymorphism (T-RFLP) analysis was performed on a SES sample and 4 subsequent IS samples taken at 5-min ..
- Molecular and geographic patterns of tuberculosis transmission after 15 years of directly observed therapyW R Bishai
Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205 2103, USA
JAMA 280:1679-84. 1998..Improved tuberculosis control measures such as uniform implementation of directly observed therapy might reduce the proportion of cases resulting from recent transmission...
- GENETIC LINKAGE OF AUDITORY GATINGKaren Stevens; Fiscal Year: 2001..proposal would extent these studies as well as assess the relationship between an identified restriction fragment length polymorphism (RFLP), near the alpha7 nicotinic receptor gene in the mouse, and auditory gating (Expt. 1)...
- IMPACT OF SOCIAL NETWORKS ON SYPHILIS TRANSMISSIONAnne Rompalo; Fiscal Year: 2004..The investigators will collect specimens from these patients' syphilis lesions for restriction fragment length polymorphism (RFLP) analysis, and conduct social and sexual network interviews with these syphilis patients ..
- Interleukin-1 Composite Genotype and Alveolar Bone LossMartha Nunn; Fiscal Year: 2005..and ApaI polymorphisms of the IL-1 gene cluster will be determined with polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) from mononuclear cells obtained from a blood draw collected during routine study ..
- Cytochrome-P-450 genes in pathobiology of endometrial cancerRajvir Dahiya; Fiscal Year: 2009..CYP1B1 genes in blood and tissue DNA from benign and endometrial cancer patients using PCR-RFLP (restriction fragment length polymorphism) and direct genomic sequencing, (b) Analyze whether SNPs of CYP1A1 and CYP1B1 genes are ..
- NIH Director's Pioneer AwardKarl Deisseroth; Fiscal Year: 2009..abstract_text> ..
- Intestinal Microbiota, Diet and Risk of Colorectal AdenomasRobert S Sandler; Fiscal Year: 2013..These methods include PCR amplification of the 16S rRNA gene, terminal restriction fragment length polymorphism (TRFLP), generation of bacteria clone libraries and sequencing...
- MOLECULAR MARKERS OF PROGNOSIS IN MEDULLOBLASTOMASANDRA BIGNER; Fiscal Year: 2000..Cytogenetic analysis and restriction fragment length polymorphism analysis using Microsatellite probes have shown that approximately 1/3 of these tumors have loss ..
- GENETIC ANALYSIS OF NORMAL AND MALIGNANT LYMPHOCYTESEdmond Yunis; Fiscal Year: 1991..The new markers will be studied by restriction fragment length polymorphism (RFLP), mixed lymphocyte reaction, T cell clones, antibody blocking and biochemical techniques...
- DIETARY CHOLESTEROL EFFECTS ON PLASMA LIPOPROTEINSPHYLLIS MCPHERSON; Fiscal Year: 1990..The relationship between a specific restriction fragment length polymorphism adjacent to the apolipoprotein AII gene and the metabolic responses to dietary cholesterol will ..
- ASSAY FOR PNEUMOCYSTOSIS IN IMMUNODEFICIENT HOSTSDON GRAVES; Fiscal Year: 1993..Use of monoclonal antibodies and Pneumocystis-specific DNA clones as diagnostic probes for early detection of pneumocystosis would improve therapeutic intervention and prognosis of individuals in a life-threatening situation...
- ETIOLOGY AND DEVELOPMENT OF CONGENITAL HEART DISEASESDonald Patterson; Fiscal Year: 1999..gel electrophoresis (1D, 2D PAGE) and antibodies against these proteins, DNA Southern blot restriction fragment length polymorphism (RFLP) studies of linkage of the CTD gene to myosin genes and other genes...
- BIOMEDICAL RESEARCH SUPPORTPeter Henson; Fiscal Year: 1991..Local commitment of funds by the Cancer Center at Duke to support operating costs means that user fees will be modest. No comparable instrumentation is available in North Carolina...
- MOLECULAR GENETIC ANALYSIS OF ANKYLOSING SPONDYLITISJohn Reveille; Fiscal Year: 1992..Having identified by restriction fragment length polymorphism (RFLP) analysis a 9...
- MOLECULAR GENETICS OF IDURONATE SULFATASE DEFICIENCIESJoseph Muenzer; Fiscal Year: 1991..b>Restriction fragment length polymorphism studies at the iduronate sulfatase gene locus should allow improved carrier detection in Hunter ..
- COLLAGEN BIOSYNTHESIS BY CULTURED FIBROBLASTSJouni Uitto; Fiscal Year: 1990..in total genomic DNA by denaturing gradient gel electrophoresis; e) linkage analyses utilizing restriction fragment length polymorphism of the procollagen genes...
- Molecular Pathophysiology of Human Growth DisordersMICHAEL WAJNRAJCH; Fiscal Year: 2002..an automated Single Nucleotide Polymorphism (SNP) analyzer, or alternatively, through the use of restriction fragment length polymorphism (RFLP) analysis (including induced-mutagenesis, when necessary)...
- RECEPTOR MUTANTS IN VITAMIN D RESISTANT RICKETS OF BONEMark Hughes; Fiscal Year: 1991..Screened for receptor containing sequences and sequenced for mutation identification. Restriction fragment length polymorphism (RFlP), polymerase chain reaction (POR) amplification, and ribonuclease A digestion will be ..
- CLONALITY IN ACUTE MYELOID LEUKEMIA AND MYELODYSPLASIADonald Kufe; Fiscal Year: 1992..has identified clonal growth of leukemic cells or their progeny via noting allelic loss of a restriction fragment length polymorphism (RFLP) associated with the X-linked phosphoglycerate kinase (PGK) gene...
- GENETIC DIVERSITY AND VIRULENCE OF CRYPTOSPORIDIUMDouglas Clark; Fiscal Year: 2001..DNA sequence variation of these genes will be detected by restriction fragment length polymorphism (RFLP) analysis of PCR-generated gene fragments. (2) characterize the ribosomal RNA genes from C...
- FAMILIAL PULMONARY FIBROSISDavid Riley; Fiscal Year: 1992..are to (1) recruit and clinically characterize families with pulmonary fibrosis and (2) perform restriction fragment length polymorphism (RFLP) analysis on members of these families followed by linkage analysis to candidate genes...
- GENE EXPRESSION IN FAMILIAL AND SENILE CARDIAC AMYLOIDJoel Buxbaum; Fiscal Year: 1993..It is now possible to identify many of these mutant prealbumins by restriction fragment length polymorphism (RFLP) analysis, allowing both ante-natal and presymptomatic diagnosis...
- Polymorphisms in IL-10 Locus Predispose to Preterm BirthSurendra Sharma; Fiscal Year: 2006..the association of polymorphism in the IL-10 gene promoter with preterm birth analyzed by PCR, restriction fragment length polymorphism (RFLP), and sequencing; Aim 3 tests the hypothesis that polymorphic alterations in the lL-10 gene ..
- GENETICS OF HLA-D AND T-CELL RECEPTOR HAPLOTYPESHugh McDevitt; Fiscal Year: 1991..as DNA probes for the V beta and C beta genes from the human beta-chain receptor gene complex, restriction fragment length polymorphism (RFLP) analysis for association between one maternal or paternal seventh chromosome haplotype and ..
- MOLECULAR MECHANISMS OF GENETIC REGULATION OF BONE MASSJohn Eisman; Fiscal Year: 1993..at least in part through allelic variation in the vitamin D receptor (VDR) gene as detected by restriction fragment length polymorphism (RFLP) analysis (submitted for publication...
- MOLECULAR GENETICS OF BIPOLAR AFFECTIVE DISORDERCharles Richard; Fiscal Year: 1993..In this pedigree, the genetic defect has recently been located by restriction fragment length polymorphism mapping to chromosome 11...
- APOE EXPRESSION LEVEL MODULATES VLDL METABOLISMYadong Huang; Fiscal Year: 2003..determine the allelic frequency of apoE promoter polymorphisms in normolipidemic humans by using restriction fragment length polymorphism analysis and the effect of apoE promoter polymorphisms on plasma levels of apoE and lipoproteins ..
- ALTERED SEROTONIN IN OBESITY--ONTOGENY AND MECHANISMSBarbara Horwitz; Fiscal Year: 1999..allows one to distinguish fafa from Fafa littermates at any age because of the presence of a restriction fragment length polymorphism closely linked to the fa locus...
- ENZYMES INVOLVED IN METHYLASE/DPNI CLEAVAGE STRATEGIESMichael McClelland; Fiscal Year: 1992..to three mapping strategies: (1) The production of a physical map of the human genome using restriction fragment length polymorphism probes that have already been ordered genetically...
- DETECTING AND MAPPING SEQUENCE CHANGES IN DNARosemary Elliott; Fiscal Year: 1990..The single type of genetic marker which could fulfill these requirements is the restriction fragment length polymorphism in mouse DNA. We plan to map these variants using a variety of mapping techniques...
- SINGLE BASE DIFFERENCES IN THE HUMAN FACTOR VIII GENEMark Gray; Fiscal Year: 1991..in the mapping of genes responsible for human diseases is now limited by the scarcity of useful restriction fragment length polymorphism (RFLP) DNA markers...
- Molecular Epidemiology, Virulence, and Genomic Characterization of UreaplasmasKen B Waites; Fiscal Year: 2010..organisms using PCR;(2) Refine and further develop pulsed field gel electrophoresis (PFGE) and restriction fragment length polymorphism for use in determining genetic relatedness between Ureaplasma serovars and within serovars of ..
- ISOLATION OF THE RETINOBLASTOMA SUSCEPTIBILITY GENERobert Bookstein; Fiscal Year: 1990..In addition, this gene can be used as a probe in a restriction fragment length polymorphism assay to diagnose hereditary versus non-hereditary disease, both pre- and post-natally.
- HEMATOLOGICAL DIAGNOSIS OF SICKLE CELL ANEMIAGERALD KROPP; Fiscal Year: 1993..sequence variations that are related to disease severity - alpha-globin genotypes, beta-cluster restriction fragment length polymorphism (RFLP) haplotypes, binding sites for erythroid-specific trans-acting transcription elements, and ..
- HUMAN ALCOHOL DEHYDROGENASE & ALDEHYDE DEHYDROGENASEAkira Yoshida; Fiscal Year: 1993..subjects in Caucasians, Orientals (Japanese and Koreans) and American Indians, and examination of restriction fragment length polymorphism of the loci in these populations; and 4) Exploration and characterization of the cytosolic ALDHl ..
- CYP2E1 POLYMORPHISM--MECHANISM AND IMPACT ON DISEASED McCarver; Fiscal Year: 2000..The PI recently found a restriction fragment length polymorphism (RFLP) in the regulatory region of human CYP2E1 that correlates with increased in vivo CYP2E1 ..
- CYP1A1 and CYP1B1 genes in race-related Prostate CancerRajvir Dahiya; Fiscal Year: 2007..SNPs will be analyzed by PCR-RFLP (restriction fragment length polymorphism) and direct genomic sequencing...
- NOVEL GELS FOR RAPID RESOLUTION OF HIGH MW DNAANIL DIWAN; Fiscal Year: 1992..These and further developments in restriction fragment length polymorphism (RFLP) and coarse gene mapping analyses will require simple and rapid resolution of DNA molecules ..
- EXAMINATION OF THE BACTEROIDES GINGIVALIS GENOMEKenneth Klimpel; Fiscal Year: 1990..2. Make a comparison of restriction fragment length polymorphism, which will allow an estimate of the genetic diversity which exists within the species. 3...
- Genetic Factors in the Epidemiology of HIV-1 InfectionRichard Kaslow; Fiscal Year: 2005..abstract_text> ..
- GENETICS OF THE X-LINKED LYMPHOPROLIFERATIVE SYNDROMEJAMES SKARE; Fiscal Year: 1992..It was found that the mutation which causes XLP is located near Xq26 in 5 families using the restriction fragment length polymorphism (RFLP) approach...
- FOLATE BINDERS IN HEMATOPOIESIS & CELL REPLICATIONSheldon Rothenberg; Fiscal Year: 1993..its chromosomal location, and examine different human cell lines and tissues for expression and restriction fragment length polymorphism of the gene 4) determine if transfection of the functional FBP gene into cells which express ..
- DNA LEVEL STUDY OF ALLOREACTIVE T-CELL CLONE TARGETSMassimo Trucco; Fiscal Year: 1992Using restriction fragment length polymorphism analysis (RFLP), three allelic DQ-alpha and three allelic DQ-beta patterns associated DQw1 have been recognized...
- Molecular Biology of Alcohol MetabolismHelen Thompson; Fiscal Year: 2005..In Phase I, we will research an educational PCR-based system coupled with restriction fragment length polymorphism analysis (RFLP) for the detection of two ADH3 alleles that produce enzymes with altered alcohol ..
- Phospholipid Transfer Protein Mutations In DyslipidemiasMary Engler; Fiscal Year: 2004..genotype polymorphisms revealed by mutation detection methodologies, primarily by development of restriction fragment length polymorphism assays so as to develop a means of studying case-control sample populations...
- XLR GENE STRUCTURE AND FUNCTIONMark Davis; Fiscal Year: 1990..to determine the structural linkage between this locus and the xid defect of the CBA/N mouse by restriction fragment length polymorphism analysis (RFLP's) and the introduction of portions of the XLR locus into mouse embryos carrying ..
- GENETIC ANALYSIS OF HEMOPOIETIC HISTOCOMPATIBILITYIchiro Nakamura; Fiscal Year: 1993..1) to determine the location of the Hh-1 locus in the H-2-b and H-2-d chromosomes by determining restriction fragment length polymorphism (RFLP) in a panel of H-2S/D recombinant haplotypes, 2) to identify and isolate the Hh-1 genes, ..
- BIPOLAR GENETIC LINKAGE--TWO LARGE EXTENDED PEDIGREESTheodore Reich; Fiscal Year: 1992..b>Restriction fragment length polymorphism (RFLP) studies will be conducted to test the hypothesis of genetic linkage with markers shown to ..
- CYSTIC FIBROSIS PANCREATIC ADENOCARCINOMA CELL LINESRAYMOND FRIZZELL; Fiscal Year: 1993..we will determine the structural integrity of chromosome 7 in these cells using karyotype and restriction fragment length polymorphism (RFLP) analyses...
- SOUTHERN BLOTTING FOLLOWING CAPILLARY ELECTROPHORESISEdward Davis; Fiscal Year: 1991..This process will have wide application including restriction fragment length polymorphism analysis and multiplex DNA sequencing.
- GENETIC LINKAGE STUDIES OF WAARDENBURG'S SYNDROMEAmy Liu; Fiscal Year: 1993..The experimental methods include the establishment of lymphoblastoid cell lines, isolation of high molecular weight DNA, Southern blot as well as traditional linkage analysis with restriction fragment length polymorphism.
- Helicobacter induced hepatitis and tumorigenesisJames Fox; Fiscal Year: 2009..A more complete understanding of the etiopathogenesis of hepatobiliary disease and hepatocellular carcinoma is likely to result in new strategies for treatment and prevention of these important human diseases. ..
- IN VIVO PATHOGENESIS OF HELICOBACTER PYLORIJames Fox; Fiscal Year: 2009..pylori pathogenesis and specifically evaluate the role of regulatory T cells in limiting H. pylori-induced gastric pathology. ..
- HUS Pathogenesis & clinical Outcome in an in vivo modelJames Fox; Fiscal Year: 2008..We will use this model to study the pathogenesis of HUS and importantly to develop therapeutic regimens to prevent and treat EHEC disease. [unreadable] [unreadable] [unreadable] [unreadable]..
- Mucosal Immunity to Toxoplasma gondiiL Sibley; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Calcium ATPases in Apicomplexan Parasites as Potential DL Sibley; Fiscal Year: 2006..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
- Confocal Microscope for MicrobiologyL Sibley; Fiscal Year: 2002..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
- HOST FACTORS MEDIATE INVASION BY CAMPYLOBACTER JEJUNILINDA MANSFIELD; Fiscal Year: 2002..jejuni invasion is facilitated due to a direct effect of some secreted/excreted product of the worms, and 4) Determine what host cell genes are differentially expressed during C. jejuni invasion. ..
- GENE DISCOVERY IN TOXOPLASMA GONDIIL Sibley; Fiscal Year: 2003..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
- PATHOGENESIS OF HEREDITARY TRANSTHYRETIN AMYLOIDOSISMerrill Benson; Fiscal Year: 2003....
- Probing Microb Comm - Stable Isotope & RNA/DNA AnalysesJames Tiedje; Fiscal Year: 2004..abstract_text> ..
- Microecology-murine gut-initiation & progression of IBDJames Fox; Fiscal Year: 2005..prior and subsequent to oral vaccination with Helicobacter spp. antigens and mucosal adjuvants, elucidate how these vaccine strategies influence microflora dynamics and impact gut cytokine responses. ..